FastQCFastQC Report
Fri 27 May 2016
SRR2140175_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140175_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10873208
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA160900.052.7845151
GTATCAA301150.039.133661
TCAACGC369700.031.4682314
ATCAACG371400.031.337093
TATCAAC379050.030.8524272
GTGGTAT72700.030.6430571
CAACGCA380800.030.5735875
AACGCAG392600.029.688466
TGGTATC72500.029.36062
ACGCAGA444300.026.2047447
CGCAGAG490700.023.753198
GCAGAGT518050.022.4742169
GAGTACT287200.020.93684212-13
GTACATG383600.020.5789341
TACATGG384150.020.2690452
ATACAAC114800.020.0734546
CAGAGTA506850.019.79247910-11
ACATGGG388450.019.312553
TATATAC144250.019.2373833
TATACAA133950.018.8822335
Produced by FastQC (version 0.11.5)