##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140175_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10873208
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.992782442863227	33.0	30.0	33.0	18.0	34.0
2	31.36600688591628	33.0	32.0	33.0	27.0	34.0
3	31.74406099837325	33.0	31.0	33.0	29.0	34.0
4	31.816042698714124	33.0	32.0	33.0	29.0	34.0
5	32.167779555031046	33.0	33.0	34.0	31.0	34.0
6	35.81796715375996	38.0	36.0	38.0	31.0	38.0
7	36.08491918852283	38.0	37.0	38.0	33.0	38.0
8	36.36362221710465	38.0	37.0	38.0	33.0	38.0
9	36.630090493992206	38.0	38.0	38.0	34.0	38.0
10-11	36.731846847774825	38.0	38.0	38.0	34.0	38.0
12-13	36.78005074491355	38.0	38.0	38.0	34.5	38.0
14-15	36.7897991558701	38.0	38.0	38.0	35.0	38.0
16-17	36.77643552850272	38.0	38.0	38.0	34.5	38.0
18-19	36.78553399327963	38.0	38.0	38.0	35.0	38.0
20-21	36.787526351316174	38.0	38.0	38.0	35.0	38.0
22-23	36.79630470905843	38.0	38.0	38.0	35.0	38.0
24-25	36.789198858001015	38.0	38.0	38.0	35.0	38.0
26-27	36.748457667585996	38.0	38.0	38.0	35.0	38.0
28-29	36.73363852848718	38.0	38.0	38.0	34.0	38.0
30-31	36.72431417454244	38.0	38.0	38.0	34.0	38.0
32-33	36.70488771216961	38.0	38.0	38.0	34.0	38.0
34-35	36.674046596271346	38.0	38.0	38.0	34.0	38.0
36-37	36.64883127159352	38.0	38.0	38.0	34.0	38.0
38-39	36.6394066403695	38.0	38.0	38.0	34.0	38.0
40-41	36.63065857688397	38.0	38.0	38.0	34.0	38.0
42-43	36.5942355768174	38.0	38.0	38.0	34.0	38.0
44-45	36.53270184670843	38.0	38.0	38.0	34.0	38.0
46-47	36.48820392703075	38.0	38.0	38.0	34.0	38.0
48-49	36.45997664946225	38.0	38.0	38.0	34.0	38.0
50-51	36.45053649941344	38.0	38.0	38.0	34.0	38.0
52-53	36.42411814374467	38.0	38.0	38.0	34.0	38.0
54-55	36.39446100883568	38.0	38.0	38.0	34.0	38.0
56-57	36.36766026502434	38.0	38.0	38.0	34.0	38.0
58-59	36.348436022948874	38.0	38.0	38.0	33.5	38.0
60-61	36.35068868735048	38.0	38.0	38.0	33.5	38.0
62-63	36.336037203833556	38.0	38.0	38.0	33.0	38.0
64-65	36.328358953494686	38.0	38.0	38.0	33.0	38.0
66-67	36.298871881922906	38.0	38.0	38.0	33.0	38.0
68-69	36.29255357169036	38.0	37.5	38.0	33.0	38.0
70-71	36.282484099234026	38.0	37.0	38.0	33.0	38.0
72-73	36.26465462725649	38.0	37.0	38.0	33.0	38.0
74-75	36.25502328185938	38.0	37.0	38.0	33.0	38.0
76-77	36.23578974124163	38.0	37.0	38.0	33.0	38.0
78-79	36.24782548647512	38.0	37.0	38.0	33.0	38.0
80-81	36.2008226506332	38.0	37.0	38.0	33.0	38.0
82-83	36.19995918983874	38.0	37.0	38.0	33.0	38.0
84-85	36.18271133750103	38.0	37.0	38.0	33.0	38.0
86-87	36.14789303031599	38.0	37.0	38.0	33.0	38.0
88-89	36.142065872326285	38.0	37.0	38.0	33.0	38.0
90-91	36.140255938181156	38.0	37.0	38.0	33.0	38.0
92-93	36.11700031250859	38.0	37.0	38.0	33.0	38.0
94-95	36.0973023867977	38.0	37.0	38.0	33.0	38.0
96-97	36.10575594139958	38.0	37.0	38.0	33.0	38.0
98-99	36.146551881097	38.0	37.0	38.0	33.0	38.0
100-101	35.57431047027481	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	7.0
19	15.0
20	74.0
21	530.0
22	2462.0
23	7367.0
24	17184.0
25	32079.0
26	52099.0
27	78730.0
28	109648.0
29	146844.0
30	188782.0
31	240455.0
32	304639.0
33	392986.0
34	538256.0
35	823887.0
36	1678736.0
37	6258426.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.2454907512116	18.73429626288764	14.429237443080275	25.59097554282048
2	14.552540519780363	20.33534169492573	42.50757458148506	22.60454320380885
3	18.776152181356924	26.020755364442127	29.088496199503727	26.11459625469722
4	12.891715122160818	18.643062838492558	37.219659552176324	31.245562487170297
5	14.787152957577806	36.80893763624165	34.475886217083485	13.928023189097061
6	30.826183036321943	37.1003571347113	18.201592391132408	13.871867437834354
7	27.364132094226466	32.44888720973608	22.13115945174598	18.055821244291472
8	24.89698532392648	36.58124630743751	20.047671303629986	18.474097065006024
9	25.628728890314616	17.273733749965974	20.93581765381477	36.161719705904645
10-11	24.820823332067533	26.80901320098109	27.63504824289651	20.735115224054873
12-13	25.579916782532607	24.270955058484173	27.994675531522557	22.15445262746066
14-15	22.605822614899974	25.415320180360762	26.08871393284824	25.89014327189102
16-17	22.089580186454633	28.68073065465132	26.58868017608051	22.641008982813535
18-19	22.09003543388483	27.62268044536626	28.533478804047522	21.75380531670138
20-21	23.14508897513296	26.568012649130374	28.601068276555985	21.68583009918068
22-23	22.92632517080889	26.484330948369806	28.37334997079472	22.215993910026576
24-25	22.60184014422939	26.605903786481598	28.639372459504514	22.152883609784496
26-27	22.366225552763414	26.679067504729836	28.643721709763398	22.31098523274335
28-29	22.467747728295826	26.75386652815106	28.50772154199264	22.270664201560475
30-31	22.810115889718947	26.407510930077933	28.509387279335073	22.272985900868044
32-33	22.667030761383042	26.534899495072708	28.38162208816637	22.416447655377883
34-35	22.762593742526242	26.70262688025966	28.33929473392472	22.195484643289376
36-37	22.862796845432296	26.516730633633223	28.245269039186592	22.375203481747892
38-39	22.50078501778126	26.82648094212613	28.330805183100953	22.341928856991654
40-41	22.750966786173652	26.610512226465687	28.266707083022936	22.371813904337724
42-43	22.58515658414006	26.76461493440562	27.99691961741648	22.653308864037836
44-45	22.527521151849207	26.82291886803167	27.950213558970887	22.699346421148235
46-47	22.67794658216527	26.803619585874483	27.796554670067437	22.721879161892808
48-49	22.78239361075306	27.060948838158595	27.536303367742516	22.620354183345825
50-51	22.747005390026093	27.071399562545622	27.508741274583908	22.672853772844373
52-53	23.097189447766066	27.005340089338574	27.19086689940057	22.70660356349478
54-55	23.06200849839105	27.01969912945415	27.11241827803748	22.805874094117314
56-57	23.050564480575908	27.263591958893656	26.97886043974615	22.706983120784287
58-59	22.87189255307033	27.17429587097679	27.251393100015846	22.70241847593703
60-61	22.962645669059675	27.19184214445941	27.099026867241516	22.7464853192394
62-63	22.912293118674302	27.138652741464895	27.309802207949097	22.6392519319117
64-65	23.04072193459543	26.99771593542355	27.22488748309782	22.736674646883202
66-67	23.061153634328818	27.211997608641337	26.91157074850311	22.815278008526736
68-69	23.002063113971737	27.12935745719619	27.047540312022235	22.82103911680984
70-71	22.999071008100067	26.954591285122508	27.057123517987257	22.989214188790164
72-73	22.9691727085486	27.06745727295015	26.954145950036697	23.00922406846455
74-75	22.813815766433457	27.119920537777354	27.00377068239951	23.06249301338968
76-77	22.930513013473767	27.159729257870268	26.897196341430195	23.01256138722577
78-79	22.797331362159472	27.20635133774212	26.9961724214696	23.000144878628813
80-81	22.87307810361646	27.098756431876403	27.06073598201514	22.967429482492
82-83	23.145813533845764	26.962602913696248	27.145357885204696	22.746225667253288
84-85	23.074502623077226	27.015691855623587	27.05795142326643	22.85185409803276
86-87	22.838132523096615	27.227665164992853	27.012426909309244	22.921775402601288
88-89	23.11885707462714	27.065161886026257	26.815929452829344	23.000051586517255
90-91	23.124855977190478	27.106643935890496	26.90135448966519	22.867145597253838
92-93	23.141885005863568	27.134560526497907	26.990436273901018	22.733118193737504
94-95	22.76349500955752	27.423991475615672	26.975669207489055	22.836844307337753
96-97	22.860610735445487	27.354595618786608	26.958751604725883	22.826042041042026
98-99	23.20100701633458	27.94836705241862	27.005688985943593	21.844936945303203
100-101	23.87383098839211	30.366514190318366	24.381160746780324	21.3784940745092
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3500.0
1	2951.5
2	2588.5
3	3254.5
4	4446.5
5	6286.0
6	7730.5
7	8058.5
8	7731.5
9	7417.5
10	7415.0
11	8579.0
12	11010.0
13	17296.0
14	28037.5
15	40684.5
16	51766.0
17	57461.0
18	58800.0
19	54008.0
20	47495.5
21	42803.5
22	40228.0
23	43443.0
24	51381.0
25	64019.0
26	80553.0
27	98759.5
28	118446.0
29	138419.5
30	157415.5
31	177052.0
32	198799.5
33	221070.0
34	242105.0
35	262892.0
36	287666.5
37	308065.5
38	322812.0
39	339003.5
40	356557.0
41	373711.5
42	384443.0
43	393985.0
44	405609.5
45	422583.5
46	443638.0
47	464484.0
48	477496.5
49	477618.0
50	471049.0
51	468279.5
52	445347.0
53	410120.5
54	390942.0
55	372257.0
56	348903.0
57	320175.0
58	289718.5
59	254811.5
60	218188.5
61	185150.0
62	150977.0
63	125541.0
64	106613.5
65	83122.5
66	62892.5
67	44027.0
68	28518.0
69	20995.5
70	15530.0
71	10828.0
72	7773.5
73	5212.0
74	3388.5
75	2182.5
76	1333.0
77	799.5
78	504.0
79	318.0
80	199.0
81	122.5
82	88.5
83	66.0
84	42.5
85	29.0
86	24.5
87	18.5
88	11.0
89	4.5
90	2.0
91	1.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.005049107862187497
4	0.0
5	0.04278406152075818
6	0.0
7	0.0
8	0.0
9	0.0
10-11	9.196917781762292E-6
12-13	9.196917781762292E-5
14-15	4.598458890881146E-6
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	5.731007976846728E-5
46-47	0.0011804513024328764
48-49	2.4297404113939274E-5
50-51	8.817069179753442E-5
52-53	0.0014255478670017296
54-55	7.08003112621008E-4
56-57	0.004050128124245469
58-59	0.009022064443881922
60-61	0.011230965390597807
62-63	0.006393564347261351
64-65	0.008628059206022437
66-67	0.005708585052632526
68-69	0.0027926223457615936
70-71	0.004800306963913466
72-73	0.0030423272104616924
74-75	0.0013552891623272115
76-77	0.002302771327860541
78-79	8.502713083235046E-4
80-81	4.404754815735167E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	2.725578667014277E-4
94-95	2.696217530351021E-4
96-97	1.395609727326985E-4
98-99	5.448650663583023E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1312.0
20-21	4255.0
22-23	2403.0
24-25	2699.0
26-27	4580.0
28-29	8845.0
30-31	21468.0
32-33	27360.0
34-35	37358.0
36-37	57972.0
38-39	69176.0
40-41	67090.0
42-43	77127.0
44-45	91285.0
46-47	90041.0
48-49	83750.0
50-51	107081.0
52-53	72143.0
54-55	77046.0
56-57	79852.0
58-59	80746.0
60-61	78042.0
62-63	75013.0
64-65	79927.0
66-67	83777.0
68-69	86387.0
70-71	84831.0
72-73	78827.0
74-75	80863.0
76-77	84107.0
78-79	87650.0
80-81	89427.0
82-83	83469.0
84-85	82491.0
86-87	86120.0
88-89	91120.0
90-91	96487.0
92-93	91497.0
94-95	99423.0
96-97	160140.0
98-99	924874.0
100-101	7185147.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.96841994051867
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.0820221895544	73.90994626173551
2	7.484375419701649	12.419336056286042
3	1.810094239654374	4.505419770226738
4	0.657694907781378	2.1827162920618424
5	0.34504621756534565	1.4313969738924581
6	0.1756171446988303	0.8742406206076432
7	0.11647318468088949	0.6764517268889537
8	0.07410565444876939	0.49187432466179715
9	0.05450597686459803	0.4070047299993147
>10	0.19278024379558747	2.5567086397296315
>50	0.005660236732709379	0.31424987841624685
>100	0.0016030129644183779	0.2087480875468512
>500	0.0	0.0
>1k	2.157155702716062E-5	0.02190663794693178
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.839383556352458E-5	0.0	0.0	0.0
3	0.0	1.839383556352458E-5	0.0	0.0	0.0
4	0.0	4.598458890881146E-5	0.0	0.0	9.19691778176229E-6
5	0.0	5.518150669057375E-5	0.0	0.0	9.19691778176229E-6
6	0.0	5.518150669057375E-5	0.0	2.7590753345286874E-5	2.7590753345286874E-5
7	0.0	5.518150669057375E-5	0.0	2.7590753345286874E-5	2.7590753345286874E-5
8	0.0	5.518150669057375E-5	0.0	3.678767112704916E-5	2.7590753345286874E-5
9	0.0	1.1955993116290978E-4	0.0	6.437842447233604E-5	4.598458890881146E-5
10-11	0.0	1.1955993116290978E-4	0.0	8.277226003586063E-5	4.598458890881146E-5
12-13	9.19691778176229E-6	1.2415839005379094E-4	0.0	1.1496147227202864E-4	4.598458890881146E-5
14-15	9.19691778176229E-6	2.023321911987704E-4	0.0	1.1955993116290978E-4	5.977996558145489E-5
16-17	9.19691778176229E-6	2.20726026762295E-4	0.0	1.3795376672643436E-4	6.437842447233604E-5
18-19	9.19691778176229E-6	2.52915238998463E-4	0.0	1.7474143785348355E-4	6.437842447233604E-5
20-21	9.19691778176229E-6	2.8510445123463104E-4	0.0	1.9313527341700812E-4	6.437842447233604E-5
22-23	9.19691778176229E-6	3.264905812525613E-4	0.0	2.0693065008965157E-4	8.277226003586063E-5
24-25	9.19691778176229E-6	3.8627054683401625E-4	0.0	2.4371832121670073E-4	9.196917781762292E-5
26-27	9.19691778176229E-6	4.322551357428277E-4	0.0	3.080967456890368E-4	9.196917781762292E-5
28-29	9.19691778176229E-6	4.368535946337089E-4	0.0	5.748073613601433E-4	9.196917781762292E-5
30-31	9.19691778176229E-6	4.7823972465163914E-4	0.0	0.001236985441647028	1.011660955993852E-4
32-33	9.19691778176229E-6	4.7823972465163914E-4	0.0	0.002786666087873974	1.103630133811475E-4
34-35	9.19691778176229E-6	5.334212313422129E-4	0.0	0.004777798787625511	1.1955993116290978E-4
36-37	9.19691778176229E-6	5.794058202510244E-4	0.0	0.007978326175678788	1.1955993116290978E-4
38-39	9.19691778176229E-6	6.345873269415981E-4	0.0	0.012585981984341695	1.1955993116290978E-4
40-41	9.19691778176229E-6	6.897688336321719E-4	0.0	0.017727059024346816	1.1955993116290978E-4
42-43	9.19691778176229E-6	7.26556504759221E-4	0.0	0.022449676305281753	1.2875684894467207E-4
44-45	9.19691778176229E-6	8.231241414677251E-4	0.0	0.027816077830940047	1.3795376672643436E-4
46-47	9.19691778176229E-6	8.921010248309423E-4	0.0	0.03376648363574025	1.4255222561731552E-4
48-49	9.19691778176229E-6	8.921010248309423E-4	0.0	0.039923820090630106	1.4715068450819665E-4
50-51	9.19691778176229E-6	0.0010576455449026636	0.0	0.0461685272644467	1.4715068450819665E-4
52-53	1.839383556352458E-5	0.0010668424626844258	0.0	0.05202696389142928	1.5634760228995894E-4
54-55	2.7590753345286874E-5	0.0010714409215753068	0.0	0.0578762036006301	1.6554452007172126E-4
56-57	2.7590753345286874E-5	0.001076039380466188	0.0	0.06405193389108348	1.7014297896260241E-4
58-59	2.7590753345286874E-5	0.0010806378393570693	0.0	0.07004372582590161	1.7474143785348355E-4
60-61	3.218921223616802E-5	0.0010944332160297127	0.0	0.07573661793281247	1.7474143785348355E-4
62-63	3.678767112704916E-5	0.0011266224282658805	0.0	0.08122257938963368	1.9313527341700812E-4
64-65	3.678767112704916E-5	0.001259977736101434	0.0	0.08658898091529196	1.9313527341700812E-4
66-67	3.678767112704916E-5	0.0012829700305558396	0.0	0.092042753159877	1.9313527341700812E-4
68-69	3.678767112704916E-5	0.0013105607839011264	0.0	0.09766666838342465	1.9313527341700812E-4
70-71	3.678767112704916E-5	0.001328954619464651	0.0	0.10296409302571974	2.023321911987704E-4
72-73	3.678767112704916E-5	0.0013427499961372945	0.0	0.10706591835638571	2.023321911987704E-4
74-75	3.678767112704916E-5	0.0013703407494825813	0.0	0.11017447656662137	2.023321911987704E-4
76-77	3.678767112704916E-5	0.0014117268795005118	0.0	0.11337040549578375	2.0693065008965157E-4
78-79	3.678767112704916E-5	0.0014439160917366798	0.0	0.11602831473471306	2.2532448565317615E-4
80-81	3.678767112704916E-5	0.0014439160917366798	0.0	0.11715033870408806	2.4371832121670073E-4
82-83	3.678767112704916E-5	0.0014439160917366798	0.0	0.11756879846315825	2.6671061567110646E-4
84-85	3.678767112704916E-5	0.001453113009518442	0.0	0.11796426592777404	2.6671061567110646E-4
86-87	3.678767112704916E-5	0.001489900680645491	0.0	0.11817579503675456	2.6671061567110646E-4
88-89	3.678767112704916E-5	0.0015772713995722328	0.0	0.11823557500233602	2.6671061567110646E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16090	0.0	52.784515	1
GTATCAA	30115	0.0	39.13366	1
TCAACGC	36970	0.0	31.468231	4
ATCAACG	37140	0.0	31.33709	3
TATCAAC	37905	0.0	30.852427	2
GTGGTAT	7270	0.0	30.643057	1
CAACGCA	38080	0.0	30.573587	5
AACGCAG	39260	0.0	29.68846	6
TGGTATC	7250	0.0	29.3606	2
ACGCAGA	44430	0.0	26.204744	7
CGCAGAG	49070	0.0	23.75319	8
GCAGAGT	51805	0.0	22.474216	9
GAGTACT	28720	0.0	20.936842	12-13
GTACATG	38360	0.0	20.578934	1
TACATGG	38415	0.0	20.269045	2
ATACAAC	11480	0.0	20.073454	6
CAGAGTA	50685	0.0	19.792479	10-11
ACATGGG	38845	0.0	19.31255	3
TATATAC	14425	0.0	19.237383	3
TATACAA	13395	0.0	18.882233	5
>>END_MODULE