FastQCFastQC Report
Fri 27 May 2016
SRR2140174_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140174_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10719565
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA157200.050.9737131
GTATCAA290300.039.0615731
ATCAACG349050.032.2015533
TCAACGC351000.032.0333484
TATCAAC362350.031.330392
CAACGCA360000.031.2445495
AACGCAG369850.030.445496
ACGCAGA420350.026.788087
CGCAGAG430000.026.166778
GTGGTAT73600.024.952561
GCAGAGT491050.022.9664739
TGGTATC75550.022.5885752
GAGTACT297200.021.18009612-13
CAGAGTA482150.019.85715910-11
GTACATG323150.019.5693231
TACATGG316150.019.4545972
AGAGTAC450400.019.0894410-11
GTACTTT331800.018.97179614-15
ACATGGG320000.018.679113
AGTACTT323300.017.42143412-13
Produced by FastQC (version 0.11.5)