FastQCFastQC Report
Fri 27 May 2016
SRR2140173_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140173_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11093200
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA174950.052.032021
GTATCAA330200.039.1197661
ATCAACG404250.031.5085813
TCAACGC409750.031.1043244
CAACGCA414850.030.721945
TATCAAC419300.030.5309582
AACGCAG429700.029.829386
ACGCAGA478950.026.6102357
CGCAGAG486550.026.176978
ATATGCG10650.022.9363355
GCAGAGT555450.022.8913219
GTGGTAT92450.022.6226251
TGGTATC90850.022.1247312
GAGTACT351000.020.68780912-13
CAGAGTA548800.019.73008510-11
AGAGTAC517500.019.13530710-11
TACATGG358450.018.855292
ACGGTAC10600.018.5986793
GTACTTT391500.018.4821214-15
GTACATG375550.018.3619331
Produced by FastQC (version 0.11.5)