##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140173_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11093200
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.133809180398803	33.0	30.0	33.0	18.0	34.0
2	31.520328669815743	33.0	32.0	33.0	27.0	34.0
3	31.847240471640283	33.0	31.0	33.0	29.0	34.0
4	31.919705675548986	33.0	32.0	34.0	29.0	34.0
5	32.244996484332745	33.0	33.0	34.0	31.0	34.0
6	35.91539393502326	38.0	36.0	38.0	31.0	38.0
7	36.16557332419861	38.0	37.0	38.0	33.0	38.0
8	36.438558305989254	38.0	37.0	38.0	34.0	38.0
9	36.677352612411205	38.0	38.0	38.0	34.0	38.0
10-11	36.773168788086394	38.0	38.0	38.0	34.5	38.0
12-13	36.819205233836946	38.0	38.0	38.0	35.0	38.0
14-15	36.82192667580139	38.0	38.0	38.0	35.0	38.0
16-17	36.81337233620596	38.0	38.0	38.0	35.0	38.0
18-19	36.824986117621606	38.0	38.0	38.0	35.0	38.0
20-21	36.828856254437525	38.0	38.0	38.0	35.0	38.0
22-23	36.83623237386914	38.0	38.0	38.0	35.0	38.0
24-25	36.83040501749994	38.0	38.0	38.0	35.0	38.0
26-27	36.78445134833768	38.0	38.0	38.0	35.0	38.0
28-29	36.767241472004834	38.0	38.0	38.0	35.0	38.0
30-31	36.75564052403956	38.0	38.0	38.0	35.0	38.0
32-33	36.735805676946065	38.0	38.0	38.0	35.0	38.0
34-35	36.70113355339818	38.0	38.0	38.0	34.0	38.0
36-37	36.67248974734598	38.0	38.0	38.0	34.0	38.0
38-39	36.65818448144127	38.0	38.0	38.0	34.0	38.0
40-41	36.64450175440376	38.0	38.0	38.0	34.0	38.0
42-43	36.605740054677966	38.0	38.0	38.0	34.0	38.0
44-45	36.53468696116291	38.0	38.0	38.0	34.0	38.0
46-47	36.484244816840985	38.0	38.0	38.0	34.0	38.0
48-49	36.44653651236244	38.0	38.0	38.0	34.0	38.0
50-51	36.43290055611816	38.0	38.0	38.0	34.0	38.0
52-53	36.400298135918284	38.0	38.0	38.0	34.0	38.0
54-55	36.37229888654038	38.0	38.0	38.0	34.0	38.0
56-57	36.347623116908636	38.0	38.0	38.0	34.0	38.0
58-59	36.32768118689237	38.0	38.0	38.0	33.5	38.0
60-61	36.32859918959116	38.0	38.0	38.0	33.5	38.0
62-63	36.315047930000844	38.0	38.0	38.0	33.0	38.0
64-65	36.30794010872257	38.0	38.0	38.0	33.0	38.0
66-67	36.284527612901634	38.0	38.0	38.0	33.0	38.0
68-69	36.280623830815536	38.0	38.0	38.0	33.0	38.0
70-71	36.27062559528376	38.0	37.5	38.0	33.0	38.0
72-73	36.25582536399192	38.0	37.0	38.0	33.0	38.0
74-75	36.24961911311807	38.0	37.0	38.0	33.0	38.0
76-77	36.23436641969711	38.0	37.0	38.0	33.0	38.0
78-79	36.25093046896195	38.0	37.0	38.0	33.0	38.0
80-81	36.209549668074416	38.0	37.0	38.0	33.0	38.0
82-83	36.214454942275815	38.0	37.0	38.0	33.0	38.0
84-85	36.20373775810225	38.0	37.0	38.0	33.0	38.0
86-87	36.16695098144077	38.0	37.0	38.0	33.0	38.0
88-89	36.1636528580475	38.0	37.0	38.0	33.0	38.0
90-91	36.16968531399563	38.0	37.0	38.0	33.0	38.0
92-93	36.15094561247026	38.0	37.0	38.0	33.0	38.0
94-95	36.12913559906722	38.0	37.0	38.0	33.0	38.0
96-97	36.142983515545815	38.0	37.0	38.0	33.0	38.0
98-99	36.176995514124485	38.0	37.0	38.0	33.0	38.0
100-101	35.57355490723576	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	6.0
19	19.0
20	68.0
21	498.0
22	2359.0
23	6987.0
24	16619.0
25	31687.0
26	52291.0
27	79700.0
28	113746.0
29	153200.0
30	195846.0
31	245245.0
32	308081.0
33	396860.0
34	539625.0
35	819342.0
36	1655702.0
37	6475319.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.36844192838856	19.839559369703963	14.870298921862041	24.921699780045433
2	13.779315256192984	20.73208812605921	43.986108607074605	21.502488010673204
3	17.815056263604784	27.399810288042325	30.079180672242394	24.7059527761105
4	11.915804276493708	19.403760862510367	39.54091695813652	29.139517902859406
5	13.36241186144215	37.87003764757023	35.68018368803696	13.087366802950662
6	28.625301986802725	39.27549309486893	19.142393538383875	12.956811379944472
7	25.66716547073883	33.61728806836621	23.42142934410269	17.29411711679227
8	23.603486820755055	37.45209678000938	21.311902787293118	17.632513611942453
9	25.13560559622111	17.520724407745288	21.895755958605275	35.44791403742833
10-11	23.625142671907057	27.52510513989363	29.017880463940216	19.8318717242591
12-13	24.72122526386919	24.96802090941232	29.298165558569018	21.012588268149475
14-15	21.66977968485198	26.330436663902212	27.538956297551653	24.460827353694157
16-17	20.80759834853784	29.417300688710203	28.141870695561245	21.63323026719071
18-19	20.63882471418638	28.39060994080202	30.387243103308474	20.583322241703126
20-21	21.792031175072164	27.459830286139447	30.334674708940547	20.413463829847846
22-23	21.400816919699313	27.339218659107654	30.16774057446724	21.092223846725798
24-25	21.033342632142418	27.4764796331737	30.417645761537297	21.072531973146578
26-27	21.080622863270023	27.63022836576398	30.240301241163376	21.048847529802618
28-29	20.925161660981896	27.870164718542895	30.199197978924214	21.005475641551
30-31	21.366075056277055	27.545873246912162	30.150218318445564	20.937833378365216
32-33	21.097770045698255	27.6784808364476	30.090711340153	21.133037777701144
34-35	21.141629792653564	27.786862153393958	30.09253477315039	20.978973280802084
36-37	21.242243580835567	27.645288056515525	30.047393947954138	21.065074414694767
38-39	21.05491483440262	27.787363177310247	30.115771740392134	21.041950247895
40-41	21.368880726923788	27.731195212840028	29.951260596385698	20.948663463850494
42-43	21.30135872014484	27.778103335581545	29.706758924167264	21.213779020106358
44-45	21.4376311592319	28.01463836379059	29.42226104616031	21.1254694308172
46-47	21.39137452594558	27.92491170360907	29.150067820405862	21.533645950039496
48-49	21.46324500282823	27.93603478475778	29.20613387079738	21.394586341616613
50-51	21.288784178552824	28.143203135669552	28.99381203251957	21.574200653258053
52-53	21.457119731411023	28.125614924907232	28.83950470874868	21.57776063493306
54-55	21.51381361857681	28.045956256670063	28.890936077820438	21.549294046932694
56-57	21.57547928559348	28.216871442710424	28.742236717893583	21.465412553802512
58-59	21.521758303760784	28.255290203306505	28.912642097105177	21.310309395827534
60-61	21.528406178596857	28.22382963039794	28.83301394403655	21.414750246968655
62-63	21.48839809010793	28.17846856454863	28.791954064776203	21.541179280567242
64-65	21.392736179644334	28.16886768082039	28.866636233691455	21.571759905843823
66-67	21.5140217767814	28.20993389680297	28.64085100601311	21.635193320402514
68-69	21.541145383490537	28.14526606429577	28.765862710577267	21.547725841636424
70-71	21.50900163321438	27.865459358655354	28.85913499659352	21.76640401153675
72-73	21.570932318263736	27.95273024969126	28.932518544874615	21.54381888717039
74-75	21.423992892088393	28.13999097135594	28.795801380129515	21.64021475642615
76-77	21.44070876933198	28.111149572111742	28.72183829815993	21.72630336039635
78-79	21.496125386247	28.10027083786444	28.669784139290044	21.733819636598515
80-81	21.454883353965236	28.058765571844436	28.719255511227935	21.76709556296239
82-83	21.593739906564785	28.000170518120875	28.85319691585685	21.55289265945749
84-85	21.868774934126048	28.062399428219514	28.56075461117663	21.508071026477808
86-87	21.517960504104845	28.188477057792266	28.612586131386404	21.680976306716484
88-89	21.686675328188713	28.13347119995892	28.581921955356183	21.59793151649619
90-91	21.746341541328302	28.131735582388895	28.64253446155301	21.479388414729797
92-93	21.724469509866207	28.19052538381165	28.561497505345184	21.52350760097696
94-95	21.4916254871404	28.33848920215123	28.546412545163825	21.623472765544545
96-97	21.480949870371088	28.321093961614363	28.669229820454284	21.528726347560266
98-99	21.799894760861516	28.878947558738776	28.584807907399533	20.736349773000175
100-101	22.388652491711305	31.34905314471113	26.03813214981276	20.224162213764806
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2915.0
1	2939.0
2	3798.0
3	4946.0
4	6691.5
5	10386.0
6	13421.0
7	15947.5
8	18359.0
9	19040.0
10	18001.0
11	16933.5
12	18321.5
13	23527.5
14	35284.0
15	50106.0
16	61728.0
17	68917.0
18	70134.5
19	65407.5
20	58112.5
21	51141.0
22	48525.5
23	53431.0
24	64465.0
25	80698.5
26	103970.0
27	130104.0
28	155305.5
29	181407.0
30	206104.5
31	231088.0
32	261328.5
33	292558.5
34	323195.5
35	354353.0
36	385529.0
37	411233.0
38	422465.5
39	428637.0
40	443701.0
41	454731.0
42	456828.0
43	461845.0
44	467914.0
45	469885.5
46	468579.5
47	465444.0
48	457124.0
49	442410.5
50	424223.5
51	402571.0
52	376221.5
53	352125.5
54	329523.5
55	301271.5
56	271289.0
57	239571.5
58	206713.0
59	175828.0
60	143843.0
61	111974.5
62	84123.0
63	62413.5
64	46197.0
65	33950.5
66	24875.0
67	18572.0
68	13663.5
69	9784.0
70	7263.0
71	5411.5
72	3892.0
73	2777.0
74	1922.0
75	1221.0
76	753.5
77	395.5
78	231.5
79	165.0
80	118.5
81	78.0
82	47.0
83	26.5
84	18.0
85	15.0
86	13.0
87	9.0
88	5.0
89	3.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.005354631666245988
4	0.0
5	0.0435852594382144
6	0.0
7	0.0
8	0.0
9	0.0
10-11	3.1550859986297914E-5
12-13	1.2620343994519166E-4
14-15	0.0
16-17	0.0
18-19	4.507265712328273E-6
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	7.03785040401484E-5
46-47	0.0012370038761350577
48-49	4.307316915819425E-5
50-51	1.2073031797613103E-4
52-53	0.0015675623256938848
54-55	9.617061373436087E-4
56-57	0.004136485414386281
58-59	0.008908780673905088
60-61	0.011191638662817173
62-63	0.006580079054369529
64-65	0.008658054760890743
66-67	0.005889039644859914
68-69	0.0030450638159863763
70-71	0.004764631168848263
72-73	0.002848928648688297
74-75	0.001593075432121711
76-77	0.0021823373743037303
78-79	8.697456355042641E-4
80-81	5.6939416792219E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	2.4180954219378933E-4
94-95	2.386694369823782E-4
96-97	1.0883012572660736E-4
98-99	6.234507249485029E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	132.0
20-21	545.0
22-23	1158.0
24-25	1833.0
26-27	3791.0
28-29	8650.0
30-31	22316.0
32-33	28449.0
34-35	38418.0
36-37	61655.0
38-39	77955.0
40-41	76456.0
42-43	89492.0
44-45	105664.0
46-47	104514.0
48-49	96584.0
50-51	84717.0
52-53	80089.0
54-55	83916.0
56-57	87230.0
58-59	86159.0
60-61	84658.0
62-63	82538.0
64-65	85080.0
66-67	89631.0
68-69	91283.0
70-71	91802.0
72-73	85725.0
74-75	86744.0
76-77	91696.0
78-79	95442.0
80-81	96755.0
82-83	90482.0
84-85	89737.0
86-87	92627.0
88-89	96892.0
90-91	100396.0
92-93	97469.0
94-95	103800.0
96-97	162593.0
98-99	888979.0
100-101	7249148.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.56972722035799
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.07991349413207	69.2892496310046
2	8.14286803255402	12.958515762833928
3	2.3838820284110405	5.690545281785405
4	1.012879955207345	3.2237832697126745
5	0.5134967374139866	2.042939766228735
6	0.2768200476819349	1.321589740990683
7	0.1779140523374108	0.990960082920939
8	0.10380286581136217	0.660765257384121
9	0.06736173598683418	0.4823959461805951
>10	0.23667253658938994	2.7762841570370496
>50	0.0029019541384520207	0.15179121082129807
>100	0.0012481750162930364	0.17225020822154183
>500	1.0835745669580637E-4	0.060301957661197454
>1k	1.3002726318341758E-4	0.17862772721727901
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	9.014531424656546E-6	0.0
5	0.0	0.0	0.0	1.802906284931309E-5	0.0
6	0.0	0.0	0.0	1.802906284931309E-5	0.0
7	0.0	0.0	0.0	1.802906284931309E-5	0.0
8	0.0	9.014531424656546E-6	0.0	1.802906284931309E-5	0.0
9	0.0	2.704359427396964E-5	0.0	1.802906284931309E-5	0.0
10-11	0.0	2.704359427396964E-5	0.0	7.662351710958063E-5	0.0
12-13	0.0	2.704359427396964E-5	0.0	9.9159845671222E-5	0.0
14-15	0.0	7.211625139725237E-5	0.0	1.0366711138355027E-4	0.0
16-17	0.0	7.211625139725237E-5	0.0	1.0817437709587856E-4	0.0
18-19	0.0	7.662351710958063E-5	0.0	1.4873976850683301E-4	0.0
20-21	0.0	8.113078282190892E-5	0.0	1.6226156564381783E-4	0.0
22-23	0.0	8.56380485342372E-5	0.0	1.9831969134244404E-4	0.0
24-25	0.0	9.9159845671222E-5	0.0	2.524068798903833E-4	0.0
26-27	0.0	9.9159845671222E-5	0.0	2.9297227130133775E-4	0.0
28-29	0.0	9.9159845671222E-5	0.0	4.867846969314535E-4	0.0
30-31	0.0	1.0817437709587856E-4	0.0	0.0013116143222875274	0.0
32-33	0.0	1.0817437709587856E-4	0.0	0.0029387372444380343	0.0
34-35	4.507265712328273E-6	1.307107056575199E-4	0.0	0.004962499549273429	0.0
36-37	9.014531424656546E-6	1.532470342191613E-4	0.0	0.007946309450834747	0.0
38-39	9.014531424656546E-6	1.5775429993148955E-4	0.0	0.012769083763025997	9.014531424656546E-6
40-41	9.014531424656546E-6	1.7127609706847437E-4	0.0	0.017911873940792557	1.352179713698482E-5
42-43	9.014531424656546E-6	1.7127609706847437E-4	0.0	0.023347636389860454	1.802906284931309E-5
44-45	9.014531424656546E-6	2.0733422276710057E-4	0.0	0.029234125410161182	1.802906284931309E-5
46-47	9.014531424656546E-6	2.343778170410702E-4	0.0	0.035629935455955	1.802906284931309E-5
48-49	9.014531424656546E-6	2.343778170410702E-4	0.0	0.04193560018750225	1.802906284931309E-5
50-51	9.014531424656546E-6	2.4339234846572675E-4	0.0	0.04841704828183031	1.802906284931309E-5
52-53	9.014531424656546E-6	2.4339234846572675E-4	0.0	0.05445678433635019	1.802906284931309E-5
54-55	9.014531424656546E-6	2.4339234846572675E-4	0.0	0.060600187502253634	1.802906284931309E-5
56-57	9.014531424656546E-6	2.524068798903833E-4	0.0	0.06706360653373238	1.802906284931309E-5
58-59	9.014531424656546E-6	2.614214113150398E-4	0.0	0.07332419860815635	1.802906284931309E-5
60-61	9.014531424656546E-6	2.7494320845202467E-4	0.0	0.08002199545667615	1.802906284931309E-5
62-63	9.014531424656546E-6	2.9297227130133775E-4	0.0	0.08616990588829193	1.802906284931309E-5
64-65	9.014531424656546E-6	3.3353766271229223E-4	0.0	0.0919031478743735	1.802906284931309E-5
66-67	9.014531424656546E-6	3.425521941369488E-4	0.0	0.09775357876897559	1.802906284931309E-5
68-69	9.014531424656546E-6	3.921321169725598E-4	0.0	0.10352738614646811	1.802906284931309E-5
70-71	9.014531424656546E-6	4.1016117982187287E-4	0.0	0.10910738109833051	1.802906284931309E-5
72-73	9.014531424656546E-6	4.191757112465294E-4	0.0	0.11342083438502866	1.802906284931309E-5
74-75	9.014531424656546E-6	4.326975083835142E-4	0.0	0.11680579093498719	1.802906284931309E-5
76-77	9.014531424656546E-6	4.5523383694515563E-4	0.0	0.11978960083654852	1.802906284931309E-5
78-79	9.014531424656546E-6	4.687556340821404E-4	0.0	0.12269678722100025	1.802906284931309E-5
80-81	9.014531424656546E-6	4.687556340821404E-4	0.0	0.12402643060613709	1.802906284931309E-5
82-83	1.802906284931309E-5	4.687556340821404E-4	0.0	0.12463941874301374	1.802906284931309E-5
84-85	1.802906284931309E-5	4.7326289979446865E-4	0.0	0.1250946525799589	1.802906284931309E-5
86-87	1.802906284931309E-5	4.7777016550679694E-4	0.0	0.12533804492842462	1.802906284931309E-5
88-89	1.802906284931309E-5	4.957992283561101E-4	0.0	0.12542819024267118	1.802906284931309E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17495	0.0	52.03202	1
GTATCAA	33020	0.0	39.119766	1
ATCAACG	40425	0.0	31.508581	3
TCAACGC	40975	0.0	31.104324	4
CAACGCA	41485	0.0	30.72194	5
TATCAAC	41930	0.0	30.530958	2
AACGCAG	42970	0.0	29.82938	6
ACGCAGA	47895	0.0	26.610235	7
CGCAGAG	48655	0.0	26.17697	8
ATATGCG	1065	0.0	22.936335	5
GCAGAGT	55545	0.0	22.891321	9
GTGGTAT	9245	0.0	22.622625	1
TGGTATC	9085	0.0	22.124731	2
GAGTACT	35100	0.0	20.687809	12-13
CAGAGTA	54880	0.0	19.730085	10-11
AGAGTAC	51750	0.0	19.135307	10-11
TACATGG	35845	0.0	18.85529	2
ACGGTAC	1060	0.0	18.598679	3
GTACTTT	39150	0.0	18.48212	14-15
GTACATG	37555	0.0	18.361933	1
>>END_MODULE