FastQCFastQC Report
Fri 27 May 2016
SRR2140172_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140172_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10157335
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148100.049.0963821
GTATCAA263800.037.5622671
ATCAACG315650.030.718663
TCAACGC316650.030.6204414
TATCAAC322100.030.3459662
CAACGCA323850.029.9664635
GTGGTAT75350.029.5368821
TGGTATC72250.029.303032
AACGCAG334200.029.0761666
ACGCAGA381550.025.467847
CGCAGAG392650.024.7478798
GCAGAGT445450.021.7852659
GAGTACT258450.020.72850212-13
TACATGG313150.019.5481262
GTACATG322750.019.3430561
CAGAGTA441250.019.08877410-11
GTACTTT281050.018.8885214-15
ACATGGG318700.018.7312553
AGAGTAC412000.018.21803710-11
CATGGGG238400.016.7961164
Produced by FastQC (version 0.11.5)