##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140172_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10157335
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.028156401260762	33.0	30.0	33.0	18.0	34.0
2	31.461807353995905	33.0	32.0	33.0	27.0	34.0
3	31.77290214411556	33.0	31.0	33.0	29.0	34.0
4	31.833335417213274	33.0	32.0	34.0	29.0	34.0
5	32.182610399282886	33.0	33.0	34.0	31.0	34.0
6	35.802024546793035	38.0	36.0	38.0	31.0	38.0
7	36.06530817384678	38.0	37.0	38.0	33.0	38.0
8	36.33904749621825	38.0	37.0	38.0	33.0	38.0
9	36.61937909894672	38.0	38.0	38.0	34.0	38.0
10-11	36.718258578652765	38.0	38.0	38.0	34.0	38.0
12-13	36.77011312514553	38.0	38.0	38.0	34.0	38.0
14-15	36.775189505908784	38.0	38.0	38.0	34.5	38.0
16-17	36.76014801126476	38.0	38.0	38.0	34.0	38.0
18-19	36.76858885721501	38.0	38.0	38.0	34.5	38.0
20-21	36.772501821526745	38.0	38.0	38.0	35.0	38.0
22-23	36.78334161815781	38.0	38.0	38.0	35.0	38.0
24-25	36.774899051186246	38.0	38.0	38.0	35.0	38.0
26-27	36.73306786861416	38.0	38.0	38.0	34.0	38.0
28-29	36.714599504342885	38.0	38.0	38.0	34.0	38.0
30-31	36.70680434844956	38.0	38.0	38.0	34.0	38.0
32-33	36.689746315850584	38.0	38.0	38.0	34.0	38.0
34-35	36.657885743618195	38.0	38.0	38.0	34.0	38.0
36-37	36.633656529852345	38.0	38.0	38.0	34.0	38.0
38-39	36.624194360557865	38.0	38.0	38.0	34.0	38.0
40-41	36.614345366789166	38.0	38.0	38.0	34.0	38.0
42-43	36.581734041780294	38.0	38.0	38.0	34.0	38.0
44-45	36.52039111632796	38.0	38.0	38.0	34.0	38.0
46-47	36.476350074582484	38.0	38.0	38.0	34.0	38.0
48-49	36.44991818348753	38.0	38.0	38.0	34.0	38.0
50-51	36.44101184545036	38.0	38.0	38.0	34.0	38.0
52-53	36.415850446650005	38.0	38.0	38.0	34.0	38.0
54-55	36.38539573824565	38.0	38.0	38.0	34.0	38.0
56-57	36.35380766756778	38.0	38.0	38.0	34.0	38.0
58-59	36.33242461579732	38.0	38.0	38.0	33.5	38.0
60-61	36.334012682344536	38.0	38.0	38.0	33.0	38.0
62-63	36.318780119807506	38.0	38.0	38.0	33.0	38.0
64-65	36.30771112429489	38.0	38.0	38.0	33.0	38.0
66-67	36.27966605246219	38.0	37.0	38.0	33.0	38.0
68-69	36.27332227847853	38.0	37.0	38.0	33.0	38.0
70-71	36.26122427782901	38.0	37.0	38.0	33.0	38.0
72-73	36.24087511998771	38.0	37.0	38.0	33.0	38.0
74-75	36.231107797308496	38.0	37.0	38.0	33.0	38.0
76-77	36.21269299903027	38.0	37.0	38.0	33.0	38.0
78-79	36.22183078226153	38.0	37.0	38.0	33.0	38.0
80-81	36.17883667028471	38.0	37.0	38.0	33.0	38.0
82-83	36.17980990398189	38.0	37.0	38.0	33.0	38.0
84-85	36.163345498128876	38.0	37.0	38.0	33.0	38.0
86-87	36.122654146428474	38.0	37.0	38.0	33.0	38.0
88-89	36.11667239261059	38.0	37.0	38.0	33.0	38.0
90-91	36.11786663477258	38.0	37.0	38.0	33.0	38.0
92-93	36.09674708873628	38.0	37.0	38.0	33.0	38.0
94-95	36.073671390861364	38.0	37.0	38.0	33.0	38.0
96-97	36.080898077858976	38.0	37.0	38.0	33.0	38.0
98-99	36.11545593856846	38.0	37.0	38.0	33.0	38.0
100-101	35.52377017924212	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	4.0
19	13.0
20	85.0
21	470.0
22	2359.0
23	7123.0
24	15926.0
25	29859.0
26	48983.0
27	73744.0
28	103988.0
29	138720.0
30	179356.0
31	225533.0
32	286922.0
33	371632.0
34	509672.0
35	777859.0
36	1566268.0
37	5818817.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.09341672791141	19.045960382324694	14.675847552532234	25.184775337231663
2	14.372057237454511	20.112421220723743	43.35051467732432	22.165006864497432
3	19.05005043894584	25.924466226385114	28.97049415201627	26.054989182652783
4	12.70618720363166	18.562703701315357	37.86249050562967	30.868618589423306
5	14.481519350950068	37.0667270506123	34.835994649362554	13.61575894907508
6	30.543425022409913	37.47991968365718	18.3826170939523	13.594038199980604
7	27.13282568705275	32.5176830339848	22.2207498325102	18.128741446452242
8	24.147170493047636	37.48264677693509	20.289455846440035	18.08072688357724
9	25.04747554353578	17.84394233329904	21.27663407773791	35.83194804542727
10-11	24.521150162097168	27.03287728024289	28.02292550500582	20.423047052654127
12-13	25.247061958909338	24.28982131655686	28.57461276327748	21.888503961256315
14-15	22.232304154205025	25.824225834051244	26.46998087933404	25.47348913240969
16-17	21.72921342064626	29.010207894098205	27.0937012513617	22.16687743389383
18-19	21.623349037912014	27.995645511347217	29.1405176653128	21.24048778542797
20-21	22.539498047685303	27.02310586182845	29.16092762988868	21.276468460597574
22-23	22.246022031823745	26.96817546657116	28.99281248048422	21.792990021120872
24-25	21.971500187216815	27.089900966960922	29.099537127919206	21.839061717903054
26-27	21.907571656319682	27.20979809524185	29.040311229903576	21.84231901853489
28-29	21.849850238284834	27.251633694704967	29.064375995811147	21.834140071199048
30-31	22.10104626164692	27.119408584780786	28.98739109952304	21.792154054049252
32-33	21.90191654034078	27.280119920501804	28.95067138942366	21.867292149733757
34-35	22.053174106199084	27.318989395410632	28.901867157431703	21.72596934095858
36-37	22.016789141455387	27.184053353435633	28.892202149253926	21.90695535585505
38-39	21.86567304802435	27.33831792087828	28.97588033631046	21.820128694786906
40-41	22.167535756636173	27.259477226772606	28.763744111785154	21.809242904806066
42-43	21.985526850967794	27.343136998053115	28.629032217277086	22.04230393370201
44-45	22.112923487529347	27.43343452188321	28.514876642356995	21.938765348230447
46-47	22.13696811939028	27.430118552740147	28.24289799558674	22.19001533228283
48-49	22.104723224689035	27.500495776542916	28.225864352949625	22.168916645818427
50-51	22.05671865763101	27.683401261029918	28.12800563827431	22.13187444306476
52-53	22.31376895487755	27.71856338589179	27.778795301668403	22.188872357562257
54-55	22.31015806192337	27.630744799891716	27.807654634861727	22.251442503323187
56-57	22.229349813496377	27.768248027507177	27.82257418774463	22.179827971251814
58-59	22.246744098376606	27.777284447805183	27.849872978139693	22.126098475678514
60-61	22.283943133832498	27.68594398608689	27.87861439239047	22.151498487690137
62-63	22.244385994485345	27.77407006774974	27.862332732724337	22.119211205040575
64-65	22.221388316563857	27.76018420114596	27.853484498619352	22.164942983670826
66-67	22.276433080080498	27.75299980496281	27.708817232422273	22.26174988253442
68-69	22.23549552114143	27.710279778672504	27.799244708636785	22.25497999154928
70-71	22.229642065304997	27.478459342380194	27.86666973875962	22.425228853555186
72-73	22.2630881474372	27.56710887853591	27.806494297124733	22.363308676902154
74-75	22.08899332278182	27.709224750069406	27.817903416457497	22.383878510691275
76-77	22.253711694052985	27.700834397906394	27.70138262634083	22.344071281699797
78-79	22.20759384499823	27.697304239802005	27.661265925677945	22.43383598952182
80-81	22.222863223058734	27.70491843727808	27.66410065284956	22.408117686813622
82-83	22.276077604158793	27.71606403952715	27.755263927832363	22.252594428481697
84-85	22.39720881481788	27.679458362923437	27.67718005431105	22.24615276794763
86-87	22.217271218592728	27.734315244630114	27.734037768590774	22.314375768186384
88-89	22.299014690593363	27.74710442635752	27.67014282648173	22.283738056567383
90-91	22.306287108538704	27.78123765713835	27.667291249036403	22.24518398528654
92-93	22.343850631732234	27.749846728188395	27.670747719664163	22.23555492041521
94-95	22.183159372156812	27.876019086858978	27.694551461075385	22.24627007990882
96-97	22.21698323983996	27.88551485852173	27.69295153398188	22.20455036765643
98-99	22.4897115674896	28.469371603716624	27.732265425835234	21.30865140295854
100-101	23.109481403876377	30.96694732269481	25.106681536021373	20.81688973740744
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2731.0
1	2360.5
2	2159.0
3	2797.5
4	3962.0
5	5647.0
6	6954.0
7	7276.5
8	7061.5
9	6859.0
10	6864.5
11	7803.5
12	9909.0
13	15516.5
14	24979.0
15	35933.0
16	45853.5
17	51471.0
18	53281.5
19	49911.0
20	44960.5
21	40945.5
22	38944.0
23	42499.0
24	50806.0
25	63829.0
26	81135.0
27	99964.0
28	120126.0
29	142114.5
30	162305.0
31	183786.5
32	206735.0
33	229277.0
34	253445.0
35	275660.0
36	298235.0
37	320339.0
38	337835.0
39	352362.5
40	369048.5
41	385508.0
42	394334.0
43	400393.5
44	408502.0
45	417015.5
46	421240.5
47	421976.5
48	423993.0
49	422784.5
50	414719.0
51	401194.5
52	382030.5
53	359445.0
54	336319.5
55	312122.5
56	287475.0
57	260741.5
58	232406.0
59	203204.5
60	170853.0
61	138907.5
62	109495.0
63	84736.5
64	65017.5
65	49068.5
66	37219.5
67	28329.5
68	21190.0
69	15841.5
70	11784.5
71	8509.0
72	6098.0
73	4241.5
74	2878.0
75	1866.0
76	1182.5
77	737.0
78	447.0
79	296.5
80	198.5
81	129.0
82	88.0
83	61.0
84	47.0
85	31.5
86	20.5
87	17.0
88	12.5
89	8.5
90	6.5
91	4.0
92	2.5
93	2.5
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.004932396145248729
4	0.0
5	0.044558932042706084
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.4767653129487213E-5
12-13	1.427539802517097E-4
14-15	4.922551043162405E-6
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	5.093979074647119E-5
46-47	0.0010065978379716436
48-49	2.070588108622845E-5
50-51	9.907434319707065E-5
52-53	0.0014056197414291415
54-55	7.790522495504683E-4
56-57	0.0036938502730886995
58-59	0.008728578948625188
60-61	0.010685909106155675
62-63	0.006331758835969456
64-65	0.008136790890356798
66-67	0.0053964764665423446
68-69	0.002742721495847905
70-71	0.00452906638197391
72-73	0.002993009080460149
74-75	0.0013623285698389155
76-77	0.002129389311919871
78-79	8.033027618422107E-4
80-81	3.9360690736649424E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	2.7972066224426226E-4
94-95	3.0151337098732815E-4
96-97	1.4619409108908876E-4
98-99	5.241150579304373E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1057.0
20-21	3731.0
22-23	2063.0
24-25	2253.0
26-27	3745.0
28-29	6676.0
30-31	17089.0
32-33	21967.0
34-35	30913.0
36-37	49373.0
38-39	59220.0
40-41	57700.0
42-43	66827.0
44-45	79049.0
46-47	77889.0
48-49	72600.0
50-51	92243.0
52-53	62261.0
54-55	66452.0
56-57	69562.0
58-59	70179.0
60-61	68189.0
62-63	64996.0
64-65	68823.0
66-67	73039.0
68-69	74852.0
70-71	73464.0
72-73	69077.0
74-75	69304.0
76-77	73857.0
78-79	77842.0
80-81	78859.0
82-83	72929.0
84-85	71696.0
86-87	76097.0
88-89	80430.0
90-91	86838.0
92-93	82662.0
94-95	88105.0
96-97	146488.0
98-99	859509.0
100-101	6887430.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.59358537270269
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.9647469771615	72.65323839246379
2	8.245680205065922	13.620805839462339
3	2.0844984364188424	5.164985987028748
4	0.7816551572999729	2.5823880786587465
5	0.3556092139539746	1.468551998601365
6	0.1904828456104749	0.9439596702578462
7	0.10706978889025029	0.61902944246809
8	0.06723878756330226	0.44427940327732995
9	0.04970617786599753	0.3694870300613411
>10	0.1504235217556148	1.8722936368381453
>50	0.002143675168706823	0.11687508956060157
>100	6.625846617115576E-4	0.08782038209607897
>500	6.197224624396648E-5	0.030881503414007125
>1k	2.065633757878384E-5	0.02540354581156667
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.84510208632481E-6
2	0.0	2.9535306258974426E-5	0.0	0.0	9.84510208632481E-6
3	0.0	2.9535306258974426E-5	0.0	0.0	9.84510208632481E-6
4	0.0	6.891571460427366E-5	0.0	2.9535306258974426E-5	9.84510208632481E-6
5	0.0	7.876081669059847E-5	0.0	3.938040834529924E-5	9.84510208632481E-6
6	4.922551043162404E-5	7.876081669059847E-5	0.0	3.938040834529924E-5	3.938040834529924E-5
7	4.922551043162404E-5	7.876081669059847E-5	0.0	4.922551043162404E-5	3.938040834529924E-5
8	4.922551043162404E-5	7.876081669059847E-5	0.0	4.922551043162404E-5	4.922551043162404E-5
9	4.922551043162404E-5	1.3783142920854733E-4	0.0	5.907061251794885E-5	4.922551043162404E-5
10-11	7.383826564743608E-5	1.3783142920854733E-4	0.0	7.383826564743608E-5	4.922551043162404E-5
12-13	7.876081669059847E-5	1.3783142920854733E-4	0.0	8.368336773376087E-5	4.922551043162404E-5
14-15	7.876081669059847E-5	1.9690204172649617E-4	0.0	9.845102086324808E-5	7.876081669059846E-5
16-17	9.845102086324808E-5	2.06747143812821E-4	0.0	9.845102086324808E-5	9.845102086324808E-5
18-19	1.279863271222225E-4	2.4120500111495782E-4	0.0	1.082961229495729E-4	9.845102086324808E-5
20-21	1.7228928651068416E-4	2.7566285841709465E-4	0.0	1.181412250358977E-4	1.033735719064105E-4
22-23	2.06747143812821E-4	3.1504326676239384E-4	0.0	1.181412250358977E-4	1.427539802517097E-4
24-25	2.215147969423082E-4	3.445785730213683E-4	0.0	1.4767653129487212E-4	1.7721183755384654E-4
26-27	2.362824500717954E-4	3.642687771940179E-4	0.0	1.8213438859700896E-4	1.8705693964017137E-4
28-29	2.362824500717954E-4	3.642687771940179E-4	0.0	4.824100022299156E-4	1.9197949068333375E-4
30-31	2.608952052876074E-4	4.13494287625642E-4	0.0	0.0012355603118337634	2.0182459276965858E-4
32-33	2.7566285841709465E-4	4.13494287625642E-4	0.0	0.0030667492998901775	2.2643734798547059E-4
34-35	3.0027561363290666E-4	4.8241000222991564E-4	0.0	0.005798765128845312	2.4612755215812023E-4
36-37	3.150432667623939E-4	5.513257168341893E-4	0.0	0.009741728514418398	2.4612755215812023E-4
38-39	3.150432667623939E-4	5.513257168341893E-4	0.0	0.016234573340349612	2.5105010320128265E-4
40-41	3.396560219782059E-4	6.005512272658133E-4	0.0	0.023569174394661593	2.55972654244445E-4
42-43	3.445785730213683E-4	6.20241431438463E-4	0.0	0.030367217385268874	2.55972654244445E-4
44-45	3.445785730213683E-4	7.088473502153863E-4	0.0	0.03800701660425693	2.55972654244445E-4
46-47	3.544236751076931E-4	7.580728606470103E-4	0.0	0.04656241031727318	2.6581775633076983E-4
48-49	3.544236751076931E-4	7.629954116901727E-4	0.0	0.055112881479246284	2.6581775633076983E-4
50-51	3.691913282371803E-4	8.614464325534207E-4	0.0	0.06380610662147108	2.6581775633076983E-4
52-53	3.7903643032350515E-4	8.663689835965832E-4	0.0	0.0719480060468617	2.6581775633076983E-4
54-55	3.8395898136666756E-4	8.712915346397456E-4	0.0	0.08020312414624506	2.6581775633076983E-4
56-57	3.8395898136666756E-4	8.860591877692328E-4	0.0	0.08672058172739208	2.7074030737393224E-4
58-59	3.9380408345299233E-4	8.860591877692328E-4	0.0	0.0922289163446908	2.7566285841709465E-4
60-61	4.0857173658247957E-4	9.0082684089872E-4	0.0	0.09752065871609039	2.7566285841709465E-4
62-63	4.331844917982916E-4	9.25439596114532E-4	0.0	0.10255642843324553	2.855079605034194E-4
64-65	4.331844917982916E-4	0.0010435808211504297	0.0	0.10744452161910581	2.9043051154658184E-4
66-67	4.5287469597094117E-4	0.001048503372193592	0.0	0.11274610909259171	2.9535306258974425E-4
68-69	4.62719798057266E-4	0.0010632710253230793	0.0	0.11811168972963873	2.9535306258974425E-4
70-71	4.676423491004284E-4	0.0010878837805388913	0.0	0.12317207220200968	2.9535306258974425E-4
72-73	5.1194530848889E-4	0.0011026514336683786	0.0	0.12710026793445328	3.150432667623939E-4
74-75	5.1194530848889E-4	0.001132186739927353	0.0	0.1302014750916456	3.150432667623939E-4
76-77	5.1194530848889E-4	0.0011961799034884643	0.0	0.1328596526549533	3.150432667623939E-4
78-79	5.1194530848889E-4	0.0012207926587042763	0.0	0.13551783021826097	3.150432667623939E-4
80-81	5.316355126615397E-4	0.0012207926587042763	0.0	0.13657125614149773	3.2488836884871867E-4
82-83	5.414806147478645E-4	0.0012207926587042763	0.0	0.13695029257182126	3.445785730213683E-4
84-85	5.414806147478645E-4	0.0012355603118337634	0.0	0.13727518094066998	3.445785730213683E-4
86-87	5.414806147478645E-4	0.0012503279649632507	0.0	0.13737855451257636	3.445785730213683E-4
88-89	5.414806147478645E-4	0.0012995534753948747	0.0	0.13740808981883534	3.445785730213683E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14810	0.0	49.096382	1
GTATCAA	26380	0.0	37.562267	1
ATCAACG	31565	0.0	30.71866	3
TCAACGC	31665	0.0	30.620441	4
TATCAAC	32210	0.0	30.345966	2
CAACGCA	32385	0.0	29.966463	5
GTGGTAT	7535	0.0	29.536882	1
TGGTATC	7225	0.0	29.30303	2
AACGCAG	33420	0.0	29.076166	6
ACGCAGA	38155	0.0	25.46784	7
CGCAGAG	39265	0.0	24.747879	8
GCAGAGT	44545	0.0	21.785265	9
GAGTACT	25845	0.0	20.728502	12-13
TACATGG	31315	0.0	19.548126	2
GTACATG	32275	0.0	19.343056	1
CAGAGTA	44125	0.0	19.088774	10-11
GTACTTT	28105	0.0	18.88852	14-15
ACATGGG	31870	0.0	18.731255	3
AGAGTAC	41200	0.0	18.218037	10-11
CATGGGG	23840	0.0	16.796116	4
>>END_MODULE