FastQCFastQC Report
Fri 27 May 2016
SRR2140171_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140171_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11186751
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA167000.052.9537961
GTATCAA305350.038.189581
TCAACGC371650.031.0142214
ATCAACG372800.030.8968493
TATCAAC383950.030.1912542
CAACGCA382350.030.1349745
AACGCAG396350.029.1217376
GTGGTAT75400.026.1207281
ACGCAGA446250.025.8362397
CGCAGAG453600.025.3889838
TGGTATC74400.025.3082182
GCAGAGT517800.022.1909719
GAGTACT320350.020.98633412-13
GTACATG345150.020.1034641
CAGAGTA512450.019.72956310-11
TACATGG341550.019.7070352
ACATGGG347150.018.915323
AGAGTAC479550.018.88189710-11
GTACTTT356000.018.86095414-15
AGTACTT349100.017.26281212-13
Produced by FastQC (version 0.11.5)