##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2140171_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11186751 Sequences flagged as poor quality 0 Sequence length 19-101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.31179419296988 33.0 31.0 33.0 18.0 34.0 2 31.560919162319784 33.0 32.0 33.0 27.0 34.0 3 31.84269981516528 33.0 31.0 33.0 29.0 34.0 4 31.91156878346537 33.0 32.0 34.0 29.0 34.0 5 32.227727782624285 33.0 33.0 34.0 31.0 34.0 6 35.87586368910866 38.0 36.0 38.0 31.0 38.0 7 36.139608184717794 38.0 37.0 38.0 33.0 38.0 8 36.400261434262724 38.0 37.0 38.0 34.0 38.0 9 36.64624921033819 38.0 38.0 38.0 34.0 38.0 10-11 36.74121060708333 38.0 38.0 38.0 34.0 38.0 12-13 36.78762193777264 38.0 38.0 38.0 35.0 38.0 14-15 36.791682812999056 38.0 38.0 38.0 35.0 38.0 16-17 36.780366435259 38.0 38.0 38.0 35.0 38.0 18-19 36.78848733649296 38.0 38.0 38.0 35.0 38.0 20-21 36.792652759666 38.0 38.0 38.0 35.0 38.0 22-23 36.803067643849374 38.0 38.0 38.0 35.0 38.0 24-25 36.79613607320614 38.0 38.0 38.0 35.0 38.0 26-27 36.75286034421622 38.0 38.0 38.0 35.0 38.0 28-29 36.73397774482935 38.0 38.0 38.0 34.0 38.0 30-31 36.72236256101233 38.0 38.0 38.0 34.0 38.0 32-33 36.70180781894024 38.0 38.0 38.0 34.0 38.0 34-35 36.66770539539742 38.0 38.0 38.0 34.0 38.0 36-37 36.63887000050146 38.0 38.0 38.0 34.0 38.0 38-39 36.62718928793265 38.0 38.0 38.0 34.0 38.0 40-41 36.61336115272859 38.0 38.0 38.0 34.0 38.0 42-43 36.57602423118131 38.0 38.0 38.0 34.0 38.0 44-45 36.50922627097799 38.0 38.0 38.0 34.0 38.0 46-47 36.458865370959145 38.0 38.0 38.0 34.0 38.0 48-49 36.4237629032893 38.0 38.0 38.0 34.0 38.0 50-51 36.41108440490821 38.0 38.0 38.0 34.0 38.0 52-53 36.37852917027918 38.0 38.0 38.0 34.0 38.0 54-55 36.35066011909656 38.0 38.0 38.0 34.0 38.0 56-57 36.323758729773054 38.0 38.0 38.0 33.0 38.0 58-59 36.30485276006419 38.0 38.0 38.0 33.0 38.0 60-61 36.307416617133796 38.0 38.0 38.0 33.0 38.0 62-63 36.295023276940455 38.0 38.0 38.0 33.0 38.0 64-65 36.2872524493047 38.0 38.0 38.0 33.0 38.0 66-67 36.26154680081315 38.0 37.0 38.0 33.0 38.0 68-69 36.25736563500469 38.0 37.0 38.0 33.0 38.0 70-71 36.246353427390176 38.0 37.0 38.0 33.0 38.0 72-73 36.228916410629616 38.0 37.0 38.0 33.0 38.0 74-75 36.222749105562215 38.0 37.0 38.0 33.0 38.0 76-77 36.20323390733326 38.0 37.0 38.0 33.0 38.0 78-79 36.217010302480986 38.0 37.0 38.0 33.0 38.0 80-81 36.175038260288005 38.0 37.0 38.0 33.0 38.0 82-83 36.17928838608156 38.0 37.0 38.0 33.0 38.0 84-85 36.16706464366057 38.0 37.0 38.0 33.0 38.0 86-87 36.12748109046161 38.0 37.0 38.0 33.0 38.0 88-89 36.12246262985758 38.0 37.0 38.0 33.0 38.0 90-91 36.12737862123038 38.0 37.0 38.0 33.0 38.0 92-93 36.10999971564084 38.0 37.0 38.0 33.0 38.0 94-95 36.08850182948207 38.0 37.0 38.0 33.0 38.0 96-97 36.10108911525028 38.0 37.0 38.0 33.0 38.0 98-99 36.13503840268246 38.0 37.0 38.0 33.0 38.0 100-101 35.55097186826609 38.0 36.0 38.0 30.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 6.0 19 17.0 20 84.0 21 574.0 22 2519.0 23 7510.0 24 17453.0 25 32552.0 26 54342.0 27 81786.0 28 116479.0 29 156155.0 30 201394.0 31 252973.0 32 318828.0 33 409087.0 34 554974.0 35 836729.0 36 1671575.0 37 6471713.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.99789116607673 19.33201159121178 14.627339072801387 25.0427581699101 2 14.295026321762236 20.34835673020701 43.177943265207205 22.178673682823547 3 18.50915845649008 26.451976311209357 29.467123788654593 25.571741443645973 4 12.628304679347918 18.677433689191794 38.16715863256454 30.52710299889575 5 14.174038992272159 37.316636838721976 34.94392012525948 13.56540404374638 6 30.10884035945736 37.837170059474815 18.47169924493716 13.58229033613066 7 26.654066046522356 32.95092560833793 22.566793522086975 17.828214823052736 8 24.19142966532463 37.19630480735649 20.529991236955215 18.08227429036366 9 25.263751736317364 17.529611591426324 21.403354736330506 35.80328193592581 10-11 24.361378416430153 27.045580709321293 28.27280935034751 20.320231523901043 12-13 25.14442550534848 24.483490984322863 28.7222340815051 21.649849428823565 14-15 22.192520419914594 25.78398768328713 26.69722871278712 25.326263184011154 16-17 21.5661410538234 28.992671777533978 27.336118413648432 22.10506875499419 18-19 21.54554526153304 27.92417566101185 29.4096293016623 21.12064977579281 20-21 22.378058278507414 26.960667248168953 29.500687991843773 21.160586481479868 22-23 22.152756809694633 26.85140084923251 29.336685445377732 21.659156895695126 24-25 21.858347834257895 27.00611868534765 29.423735656914456 21.71179782347999 26-27 21.781545722213743 27.127550129396038 29.377528199000828 21.713375949389388 28-29 21.728591129301662 27.199841024250375 29.371601655183373 21.69996619126459 30-31 21.992198195104034 27.022946822056927 29.301181650492964 21.683673332346075 32-33 21.768206348429704 27.196191464205977 29.26982658258566 21.76577560477866 34-35 21.93292134035401 27.186852308662786 29.243680834252515 21.63654551673069 36-37 21.930632656023242 27.097399533308913 29.20006751189478 21.77190029877307 38-39 21.768296510737613 27.258120614291325 29.25148848294486 21.722094392026204 40-41 22.069718910221557 27.13937653212245 29.06593815556655 21.724966402089443 42-43 21.91660983791752 27.23293817261704 28.89807333319557 21.952378656269875 44-45 22.006127931642855 27.306500548123267 28.78399269989472 21.90337882033915 46-47 22.09428444911068 27.333299854910237 28.515332433274015 22.057083262705063 48-49 22.01696535259543 27.461842594704972 28.443278777042668 22.077913275656925 50-51 22.021743867242396 27.585889537518586 28.319700344496994 22.072666250742028 52-53 22.194432557265674 27.55722840387373 28.167012005402842 22.08132703345775 54-55 22.162631688649046 27.43306520320359 28.242046801359134 22.162256306788237 56-57 22.125127013094804 27.555251757158967 28.236067649850234 22.083553579895995 58-59 22.144888228152286 27.593695223508252 28.23641909658066 22.0249974517588 60-61 22.18837754632577 27.51894603175104 28.281498430740687 22.0111779911825 62-63 22.1611469118243 27.600312075390736 28.2437416071386 21.994799405646358 64-65 22.11707364728862 27.53946028576945 28.227593577449305 22.11587248949262 66-67 22.1629371036953 27.578767668525455 28.113070488904164 22.145224738875083 68-69 22.099468128374465 27.51052356109957 28.191313744192787 22.198694566333177 70-71 22.10416495339745 27.317682772073614 28.230486927334255 22.34766534719468 72-73 22.157931235262726 27.395381226603423 28.163986634715847 22.282700903418007 74-75 22.012127349345334 27.537663503188313 28.160593018529685 22.289616128936668 76-77 22.16295520412152 27.4971924831052 28.056296612993272 22.283555699780013 78-79 22.095224813447235 27.505156515893248 28.02501848361439 22.37460018704513 80-81 22.150160853338964 27.53208613828404 28.00955825819435 22.308194750182643 82-83 22.19646374190162 27.502566512845107 28.10259492921452 22.198374816038754 84-85 22.299573498596235 27.528655220509602 27.99146207943441 22.18030920145976 86-87 22.130168923091496 27.57706875341142 28.037021067687434 22.25574125580965 88-89 22.20072085837618 27.55417477299758 27.968672252027883 22.276432116598354 90-91 22.234312431226343 27.633748925793405 27.966212724515604 22.16572591846465 92-93 22.256104892338843 27.57205136663054 27.985908876065796 22.18593486496482 94-95 22.09758090325213 27.68107329509845 28.016319181067605 22.205026620581812 96-97 22.185453356488047 27.682888809990764 28.001408468004442 22.13024936551675 98-99 22.439986073231168 28.25228164300868 28.031038178574658 21.27669410518549 100-101 23.00995877999779 30.737270395392823 25.448472910555413 20.804297914053972 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2944.0 1 2798.0 2 3461.0 3 4414.5 4 5286.0 5 7593.0 6 10115.5 7 13378.0 8 17208.0 9 18846.5 10 17687.5 11 16222.5 12 17275.0 13 21282.0 14 29635.0 15 40414.0 16 49383.5 17 54959.5 18 56101.0 19 53011.0 20 48255.5 21 43956.0 22 42685.5 23 47024.5 24 56471.0 25 71478.5 26 90883.5 27 111757.0 28 135271.5 29 161110.0 30 186031.5 31 211080.0 32 235991.5 33 261411.0 34 285638.5 35 307218.0 36 333225.5 37 357865.5 38 375455.5 39 389209.0 40 404215.5 41 420001.0 42 428855.0 43 434887.0 44 442644.5 45 450733.0 46 456433.5 47 457909.5 48 454678.0 49 448232.5 50 440596.0 51 430990.5 52 414925.0 53 394581.0 54 373398.0 55 346900.0 56 318591.0 57 289964.0 58 257875.0 59 223102.5 60 187461.0 61 153139.5 62 121622.0 63 94187.0 64 71813.5 65 54421.0 66 41057.0 67 31241.0 68 23637.5 69 17698.0 70 13242.5 71 9640.0 72 6974.0 73 4890.0 74 3268.0 75 2110.5 76 1327.5 77 818.5 78 531.5 79 332.0 80 209.0 81 139.0 82 102.5 83 76.0 84 50.5 85 33.5 86 22.5 87 14.0 88 9.0 89 7.5 90 5.0 91 2.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.004755625650378738 4 0.0 5 0.043292283881173364 6 0.0 7 0.0 8 0.0 9 0.0 10-11 8.93914595935853E-6 12-13 1.3408718939037797E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 5.0895647728087685E-5 46-47 0.0010127431557814125 48-49 1.4120298352490657E-5 50-51 1.0911249697687223E-4 52-53 0.0012471029344905428 54-55 6.208199925212848E-4 56-57 0.003671247497659277 58-59 0.008911328033268436 60-61 0.01138721676269262 62-63 0.006490389282822908 64-65 0.008332337873348165 66-67 0.005515255560190007 68-69 0.0025654769267418904 70-71 0.004632996085118308 72-73 0.0030884423130277234 74-75 0.0012542889496046038 76-77 0.0020353654770271833 78-79 6.972891579892758E-4 80-81 3.4940533362403856E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 2.113378531455526E-4 94-95 2.3102826232265767E-4 96-97 1.4622777666807143E-4 98-99 4.164253109821976E-4 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 120.0 20-21 442.0 22-23 983.0 24-25 1522.0 26-27 3179.0 28-29 7044.0 30-31 19058.0 32-33 24560.0 34-35 34560.0 36-37 55115.0 38-39 67441.0 40-41 66517.0 42-43 77464.0 44-45 91784.0 46-47 91918.0 48-49 85859.0 50-51 76842.0 52-53 73578.0 54-55 78510.0 56-57 81308.0 58-59 81435.0 60-61 79589.0 62-63 76942.0 64-65 80677.0 66-67 85712.0 68-69 88305.0 70-71 86835.0 72-73 81750.0 74-75 83760.0 76-77 87565.0 78-79 91452.0 80-81 93270.0 82-83 87401.0 84-85 86356.0 86-87 89531.0 88-89 94141.0 90-91 96897.0 92-93 95968.0 94-95 101756.0 96-97 167452.0 98-99 940051.0 100-101 7472102.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.03696773662533 #Duplication Level Percentage of deduplicated Percentage of total 1 87.93394535943602 72.13834238406228 2 8.179330499946452 13.420149446626054 3 2.0609803690548123 5.072297401259034 4 0.7835562481784587 2.571223146065896 5 0.3923703536688041 1.6094437022367978 6 0.21200731037132092 1.0435462128516462 7 0.1253967763006671 0.7201019904158256 8 0.08216160999014657 0.5392231478360678 9 0.04956065039011311 0.3659224929353889 >10 0.1765879274471403 2.1526411510550054 >50 0.0032815769351287306 0.17764513893301073 >100 7.420653922213688E-4 0.11419310827818258 >500 1.9816554755408218E-5 0.008258505520652106 >1k 5.943633403389061E-5 0.06701217192417859 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.93914595935853E-6 2 0.0 1.787829191871706E-5 0.0 1.787829191871706E-5 8.93914595935853E-6 3 0.0 1.787829191871706E-5 0.0 1.787829191871706E-5 8.93914595935853E-6 4 0.0 7.151316767486824E-5 0.0 4.469572979679265E-5 8.93914595935853E-6 5 0.0 7.151316767486824E-5 0.0 6.257402171550971E-5 8.93914595935853E-6 6 4.469572979679265E-5 7.151316767486824E-5 0.0 6.257402171550971E-5 8.93914595935853E-6 7 4.469572979679265E-5 7.151316767486824E-5 0.0 6.257402171550971E-5 8.93914595935853E-6 8 4.469572979679265E-5 8.045231363422678E-5 0.0 7.151316767486824E-5 1.787829191871706E-5 9 4.469572979679265E-5 1.3408718939037797E-4 0.0 8.045231363422678E-5 1.787829191871706E-5 10-11 5.810444873583045E-5 1.3408718939037797E-4 0.0 1.028001785326231E-4 1.787829191871706E-5 12-13 7.151316767486824E-5 1.430263353497365E-4 0.0 1.0726975151230236E-4 1.787829191871706E-5 14-15 7.151316767486824E-5 2.056003570652462E-4 0.0 1.0726975151230236E-4 3.575658383743412E-5 16-17 1.430263353497365E-4 2.056003570652462E-4 0.0 1.162088974716609E-4 7.151316767486824E-5 18-19 1.430263353497365E-4 2.2794822196364251E-4 0.0 1.2067847045134016E-4 8.93914595935853E-5 20-21 1.430263353497365E-4 2.5029608686203883E-4 0.0 1.2514804343101942E-4 8.93914595935853E-5 22-23 1.4749590832941573E-4 2.6817437878075593E-4 0.0 1.385567623700572E-4 1.028001785326231E-4 24-25 1.51965481309095E-4 2.994613896385108E-4 0.0 1.6090462726845356E-4 1.162088974716609E-4 26-27 1.51965481309095E-4 3.1287010857754857E-4 0.0 2.458265138823596E-4 1.162088974716609E-4 28-29 1.51965481309095E-4 3.218092545369071E-4 0.0 3.575658383743412E-4 1.162088974716609E-4 30-31 1.51965481309095E-4 3.6650498433369975E-4 0.0 0.001296176164106987 1.162088974716609E-4 32-33 1.51965481309095E-4 3.7097455731337903E-4 0.0 0.0030169617612835044 1.2514804343101942E-4 34-35 1.51965481309095E-4 4.0673114115081317E-4 0.0 0.0053009135538996085 1.2514804343101942E-4 36-37 1.6090462726845356E-4 4.38018152008568E-4 0.0 0.008836345780825906 1.2514804343101942E-4 38-39 1.6090462726845356E-4 4.648355898866436E-4 0.0 0.013994232999375779 1.2514804343101942E-4 40-41 1.6984377322781208E-4 5.095313196834362E-4 0.0 0.019679529829527805 1.2514804343101942E-4 42-43 1.787829191871706E-4 5.318791845818325E-4 0.0 0.025212861178370738 1.2514804343101942E-4 44-45 1.787829191871706E-4 5.899836333176629E-4 0.0 0.03154177651759657 1.2514804343101942E-4 46-47 1.787829191871706E-4 6.257402171550971E-4 0.0 0.038415979760343284 1.296176164106987E-4 48-49 1.787829191871706E-4 6.257402171550971E-4 0.0 0.045254426419252564 1.51965481309095E-4 50-51 1.9666121110588767E-4 6.883142388706069E-4 0.0 0.0519274988779137 1.51965481309095E-4 52-53 2.056003570652462E-4 7.061925307893239E-4 0.0 0.05857375389869677 1.6984377322781208E-4 54-55 2.056003570652462E-4 7.106621037690031E-4 0.0 0.06561780091467129 1.8772206514652915E-4 56-57 2.056003570652462E-4 7.330099686673995E-4 0.0 0.07270207408746293 1.9219163812620843E-4 58-59 2.056003570652462E-4 7.59827406545475E-4 0.0 0.07867342358831443 2.0113078408556692E-4 60-61 2.1006993004492547E-4 8.134622823016262E-4 0.0 0.08452856419169427 2.1453950302460472E-4 62-63 2.413569409026803E-4 8.224014282609848E-4 0.0 0.09000826066478104 2.413569409026803E-4 64-65 2.413569409026803E-4 9.296711797732872E-4 0.0 0.09524660019696514 2.413569409026803E-4 66-67 2.4582651388235956E-4 9.475494716920043E-4 0.0 0.10120901055185727 2.547656598417181E-4 68-69 2.5029608686203883E-4 9.60958190631042E-4 0.0 0.10695688140372481 2.6817437878075593E-4 70-71 2.547656598417181E-4 9.743669095700798E-4 0.0 0.11232930812529929 2.86052670699473E-4 72-73 2.592352328213974E-4 9.833060555294384E-4 0.0 0.1165083588612994 3.218092545369071E-4 74-75 2.592352328213974E-4 0.0010056539204278347 0.0 0.11980243414732303 3.218092545369071E-4 76-77 2.6370480580107666E-4 0.0010324713583059102 0.0 0.12253781281088674 3.218092545369071E-4 78-79 2.6817437878075593E-4 0.0010369409312855895 0.0 0.12504971282546648 3.218092545369071E-4 80-81 2.6817437878075593E-4 0.0010414105042652689 0.0 0.12593915784842263 3.3074840049626567E-4 82-83 2.6817437878075593E-4 0.001045880077244948 0.0 0.12633248027063443 3.486266924149827E-4 84-85 2.7264395176043515E-4 0.0010592887961839857 0.0 0.12670345482794781 3.486266924149827E-4 86-87 2.771135247401144E-4 0.0011039845259807786 0.0 0.12692693347693176 3.486266924149827E-4 88-89 2.771135247401144E-4 0.0011263323908791748 0.0 0.127007385790566 3.53096265394662E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 16700 0.0 52.953796 1 GTATCAA 30535 0.0 38.18958 1 TCAACGC 37165 0.0 31.014221 4 ATCAACG 37280 0.0 30.896849 3 TATCAAC 38395 0.0 30.191254 2 CAACGCA 38235 0.0 30.134974 5 AACGCAG 39635 0.0 29.121737 6 GTGGTAT 7540 0.0 26.120728 1 ACGCAGA 44625 0.0 25.836239 7 CGCAGAG 45360 0.0 25.388983 8 TGGTATC 7440 0.0 25.308218 2 GCAGAGT 51780 0.0 22.190971 9 GAGTACT 32035 0.0 20.986334 12-13 GTACATG 34515 0.0 20.103464 1 CAGAGTA 51245 0.0 19.729563 10-11 TACATGG 34155 0.0 19.707035 2 ACATGGG 34715 0.0 18.91532 3 AGAGTAC 47955 0.0 18.881897 10-11 GTACTTT 35600 0.0 18.860954 14-15 AGTACTT 34910 0.0 17.262812 12-13 >>END_MODULE