FastQCFastQC Report
Fri 27 May 2016
SRR2140170_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140170_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15014092
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA218050.051.551151
GTATCAA411300.039.022981
ATCAACG497000.031.8241463
TCAACGC499550.031.6603264
TATCAAC514550.031.0581132
CAACGCA513650.030.7744925
AACGCAG528400.029.9434976
GTGGTAT107500.028.0422291
TGGTATC104400.027.1763782
ACGCAGA600300.026.3211427
CGCAGAG609300.025.9110648
GCAGAGT696250.022.6876249
GAGTACT446650.020.63498112-13
TACATGG436600.019.6835022
CAGAGTA685850.019.61535310-11
GTACATG447500.019.5133341
AGAGTAC641950.019.06108710-11
ACATGGG437350.018.8685023
GTACTTT498000.018.51203214-15
AGTACTT482450.017.58944712-13
Produced by FastQC (version 0.11.5)