##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140170_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15014092
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.81322107257635	32.0	30.0	33.0	18.0	33.0
2	31.36309868089259	33.0	32.0	33.0	27.0	34.0
3	31.71789209763734	33.0	31.0	33.0	29.0	34.0
4	31.775891276009233	33.0	32.0	33.0	29.0	34.0
5	32.15418854500159	33.0	33.0	34.0	31.0	34.0
6	35.76808554256894	38.0	36.0	38.0	31.0	38.0
7	36.02938932304398	38.0	36.0	38.0	32.0	38.0
8	36.316506119717396	38.0	37.0	38.0	33.0	38.0
9	36.610400615634966	38.0	38.0	38.0	34.0	38.0
10-11	36.71550484038595	38.0	38.0	38.0	34.0	38.0
12-13	36.76918554248901	38.0	38.0	38.0	34.0	38.0
14-15	36.77517335047634	38.0	38.0	38.0	34.5	38.0
16-17	36.761097174574395	38.0	38.0	38.0	34.0	38.0
18-19	36.771327463558904	38.0	38.0	38.0	35.0	38.0
20-21	36.77418484639853	38.0	38.0	38.0	35.0	38.0
22-23	36.782404763257745	38.0	38.0	38.0	35.0	38.0
24-25	36.77551847262116	38.0	38.0	38.0	35.0	38.0
26-27	36.73041761712021	38.0	38.0	38.0	34.0	38.0
28-29	36.70934448534684	38.0	38.0	38.0	34.0	38.0
30-31	36.69910781323293	38.0	38.0	38.0	34.0	38.0
32-33	36.68110801181811	38.0	38.0	38.0	34.0	38.0
34-35	36.646329821993675	38.0	38.0	38.0	34.0	38.0
36-37	36.61647471795544	38.0	38.0	38.0	34.0	38.0
38-39	36.602942363921535	38.0	38.0	38.0	34.0	38.0
40-41	36.58951845414628	38.0	38.0	38.0	34.0	38.0
42-43	36.54675728402351	38.0	38.0	38.0	34.0	38.0
44-45	36.47614772891852	38.0	38.0	38.0	34.0	38.0
46-47	36.423604881875086	38.0	38.0	38.0	34.0	38.0
48-49	36.3846934901635	38.0	38.0	38.0	34.0	38.0
50-51	36.36971631927687	38.0	38.0	38.0	34.0	38.0
52-53	36.332399699534975	38.0	38.0	38.0	33.5	38.0
54-55	36.30206484826571	38.0	38.0	38.0	33.0	38.0
56-57	36.273699028339436	38.0	38.0	38.0	33.0	38.0
58-59	36.25006258906423	38.0	37.5	38.0	33.0	38.0
60-61	36.25110163668133	38.0	37.0	38.0	33.0	38.0
62-63	36.23653047862476	38.0	37.0	38.0	33.0	38.0
64-65	36.226266451551936	38.0	37.0	38.0	33.0	38.0
66-67	36.19979690526257	38.0	37.0	38.0	33.0	38.0
68-69	36.19315783046137	38.0	37.0	38.0	33.0	38.0
70-71	36.18053768040431	38.0	37.0	38.0	33.0	38.0
72-73	36.16195156207836	38.0	37.0	38.0	33.0	38.0
74-75	36.15087725795281	38.0	37.0	38.0	33.0	38.0
76-77	36.132594133937474	38.0	37.0	38.0	33.0	38.0
78-79	36.145204563689944	38.0	37.0	38.0	33.0	38.0
80-81	36.10138608128038	38.0	37.0	38.0	33.0	38.0
82-83	36.10240972933161	38.0	37.0	38.0	33.0	38.0
84-85	36.08827522517883	38.0	37.0	38.0	33.0	38.0
86-87	36.047740797304826	38.0	37.0	38.0	32.5	38.0
88-89	36.03887764268376	38.0	37.0	38.0	32.5	38.0
90-91	36.04112141035157	38.0	37.0	38.0	32.5	38.0
92-93	36.02430452007215	38.0	37.0	38.0	32.5	38.0
94-95	36.001074951997566	38.0	37.0	38.0	32.0	38.0
96-97	36.012453611190224	38.0	37.0	38.0	32.5	38.0
98-99	36.04774974441534	38.0	37.0	38.0	33.0	38.0
100-101	35.42956183589659	38.0	35.5	38.0	30.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	7.0
19	20.0
20	112.0
21	800.0
22	3693.0
23	10909.0
24	24624.0
25	46339.0
26	75554.0
27	113726.0
28	160802.0
29	215748.0
30	276417.0
31	346509.0
32	437700.0
33	564351.0
34	769372.0
35	1168576.0
36	2369597.0
37	8429236.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.64090589027961	19.182938268927618	14.717193687104087	25.45896215368868
2	14.085274021232852	20.522626343304676	43.72311692242195	21.66898271304052
3	18.754811553616776	26.52401443995614	29.26472999815764	25.456444008269447
4	12.424227852073905	19.09776495308541	38.47593980375237	30.002067391088318
5	14.194451366835336	37.31227919609053	35.01881039415872	13.474459042915418
6	29.652995332651482	38.16600431114982	18.778658076692217	13.40234227950648
7	26.438382021370323	32.99197846929405	22.654157174473156	17.915482334862475
8	23.712536195995067	37.61580786903397	20.793911479961626	17.877744455009335
9	24.95458266806944	17.886929159618845	21.53164507051109	35.626843101800624
10-11	24.044528180617036	27.36967198306257	28.427305355720083	20.158494480600304
12-13	24.939211192646844	24.676164623300213	28.926370838183367	21.458253345869576
14-15	21.915284653910472	26.092350439840118	27.01886334518265	24.97350156106676
16-17	21.247065756623844	29.29397595272495	27.635457408946206	21.823500881705
18-19	21.134941094006884	28.206474290952794	29.809318472272583	20.849266142767743
20-21	22.052898102007294	27.25925512972757	29.831177418068926	20.856669350196206
22-23	21.693452553323127	27.168959755443534	29.731380014000557	21.406207677232782
24-25	21.439112451660964	27.28063549488559	29.855748480540043	21.424503572913395
26-27	21.427975590156294	27.46972905276036	29.735317058993743	21.366978298089602
28-29	21.313151039280058	27.541549707183332	29.76963911216213	21.375660141374482
30-31	21.64425988128545	27.34529084591481	29.711843379753976	21.298605893045764
32-33	21.421274237179265	27.465389565160958	29.67775592309547	21.435580274564302
34-35	21.557987388431034	27.52838574530232	29.637126775983013	21.276500090283637
36-37	21.537612135819685	27.45716696048631	29.62388856038038	21.38133234331363
38-39	21.36394136544849	27.603794999492216	29.6714460487507	21.3608175863086
40-41	21.70721430849599	27.533307453162603	29.455634692817885	21.30384354552352
42-43	21.579687371621066	27.54528866431134	29.32232013255553	21.552703831512062
44-45	21.66195849743678	27.725984102352324	29.129075683813184	21.482981716397713
46-47	21.678539286178292	27.715980263141184	28.868857390291048	21.73662306038948
48-49	21.660591294418328	27.75327962329345	28.877549379791073	21.708579702497147
50-51	21.62302936610366	27.953485677721552	28.69429727167212	21.729187684502673
52-53	21.769690471433513	27.896987661168428	28.59365194731682	21.73966992008124
54-55	21.744213581926914	27.82053056861244	28.655780070734053	21.779475778726592
56-57	21.79869129717141	27.91263255477423	28.56682117258688	21.721854975467476
58-59	21.75331702155428	27.972503383052572	28.616604356409738	21.657575238983416
60-61	21.790553611923137	27.92255183282215	28.640912198410568	21.645982356844147
62-63	21.727226274989963	27.959607441602486	28.63823410439711	21.67493217901044
64-65	21.688864794974155	27.923269757925716	28.642456189613995	21.74540925748613
66-67	21.781909726506072	27.98275221327602	28.476816107288382	21.758521952929527
68-69	21.790742832902374	27.90886602138437	28.54798424483548	21.752406900877773
70-71	21.743714053579595	27.685588847172056	28.60856311696964	21.962133982278704
72-73	21.78184996743604	27.739471647329694	28.626023596686277	21.85265478854798
74-75	21.65524897196712	27.890376881154715	28.552641234226346	21.90173291265182
76-77	21.77686523026919	27.836973466213564	28.469096068884497	21.91706523463275
78-79	21.72225949793255	27.92504262458739	28.428510720525153	21.9241871569549
80-81	21.75045336901491	27.9035901809361	28.409771942934103	21.936184507114888
82-83	21.820075350322025	27.900329035512478	28.503509552971547	21.776086061193954
84-85	21.990451288587547	27.87219301413159	28.360051925554963	21.777303771725894
86-87	21.73652573873453	27.944292684910053	28.44235918886147	21.87682238749394
88-89	21.868838015960513	27.962313860056405	28.36920445780871	21.79964366617437
90-91	21.923644403970226	27.927772937157247	28.37634755179262	21.77223510707991
92-93	21.880870141469824	27.952865748274036	28.38781902716111	21.77844508309503
94-95	21.75291970426055	28.09909009864866	28.350457446149395	21.797532750941397
96-97	21.75987905255687	28.10718009556824	28.387743693854006	21.745197158020883
98-99	22.04699824437087	28.617594917925608	28.449628844305092	20.88577799339843
100-101	22.626002051800995	31.170637880141218	25.838096859757755	20.365263208300032
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3741.0
1	3513.0
2	4350.5
3	5724.0
4	7181.0
5	10681.0
6	14178.5
7	18431.0
8	23438.0
9	25691.5
10	24329.0
11	22216.0
12	23448.5
13	29351.5
14	41970.5
15	57821.5
16	70800.5
17	79346.0
18	81125.5
19	76496.0
20	69491.5
21	63014.0
22	61108.0
23	66668.5
24	79453.5
25	99654.0
26	127633.0
27	159085.0
28	191661.0
29	226137.5
30	259952.0
31	294752.0
32	332406.5
33	370804.0
34	408010.0
35	443072.0
36	480408.0
37	513741.5
38	534452.5
39	549855.5
40	572457.5
41	594172.0
42	604783.5
43	613727.5
44	623590.0
45	628739.5
46	627461.0
47	623623.5
48	616976.0
49	602628.5
50	582224.5
51	556647.0
52	525444.5
53	493950.5
54	461093.0
55	425136.0
56	388126.0
57	348587.0
58	309010.0
59	267836.5
60	223498.0
61	179211.5
62	137692.5
63	104405.0
64	78854.0
65	58440.5
66	42929.5
67	32358.5
68	24204.0
69	17488.0
70	12784.0
71	9315.5
72	6654.0
73	4641.5
74	3186.0
75	2123.0
76	1361.5
77	832.0
78	512.0
79	314.0
80	201.5
81	133.0
82	91.0
83	63.0
84	46.0
85	34.0
86	24.0
87	16.5
88	11.5
89	8.0
90	7.0
91	6.0
92	4.5
93	3.5
94	2.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.004995307075512791
4	0.0
5	0.042839753479597696
6	0.0
7	0.0
8	0.0
9	0.0
10-11	2.997184245307675E-5
12-13	1.3653839339734965E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	4.4846805382665375E-5
46-47	0.0010197358918843293
48-49	2.8083917835724106E-5
50-51	1.5216509700701871E-4
52-53	0.001372328859897699
54-55	7.646905399702489E-4
56-57	0.0039033454962881316
58-59	0.008787561218034746
60-61	0.010854440374019093
62-63	0.0063159395198967285
64-65	0.008312804525987986
66-67	0.005268934310615269
68-69	0.0026118471335983724
70-71	0.004481818812476602
72-73	0.002917589797230987
74-75	0.0013717178970177333
76-77	0.001918357156669202
78-79	6.983360477179369E-4
80-81	4.1270744659665815E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	1.7448147191888807E-4
94-95	2.710593425055512E-4
96-97	8.713282933671746E-5
98-99	4.94173980778519E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	163.0
20-21	661.0
22-23	1427.0
24-25	2205.0
26-27	4292.0
28-29	9284.0
30-31	26122.0
32-33	34200.0
34-35	47343.0
36-37	75692.0
38-39	91892.0
40-41	90807.0
42-43	105855.0
44-45	125503.0
46-47	125680.0
48-49	116859.0
50-51	104262.0
52-53	99096.0
54-55	104123.0
56-57	108670.0
58-59	109940.0
60-61	106867.0
62-63	102066.0
64-65	107571.0
66-67	113718.0
68-69	117518.0
70-71	116270.0
72-73	108622.0
74-75	109490.0
76-77	116361.0
78-79	121686.0
80-81	123901.0
82-83	115837.0
84-85	114861.0
86-87	118969.0
88-89	125646.0
90-91	130099.0
92-93	127196.0
94-95	134932.0
96-97	221995.0
98-99	1248026.0
100-101	1.0048385E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.46371788838933
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.79117781461963	71.51795742579597
2	8.121079917418896	13.23146726683354
3	2.172463178270292	5.309307824325741
4	0.8353296332418507	2.721962303449034
5	0.41247459982125395	1.6800857217982454
6	0.21406622102067563	1.0463178143197147
7	0.12634187151893614	0.720459500523682
8	0.08073070162950709	0.5261298481982318
9	0.055863338713678705	0.40957517387471937
>10	0.18480829001301857	2.2785057247437357
>50	0.004238465076802787	0.23247032356127922
>100	0.0013029745967317332	0.17632463599557038
>500	6.150091354787728E-5	0.037512583641038225
>1k	6.149314529469685E-5	0.11192385293942425
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.660409434017056E-6	0.0	0.0	0.0
3	0.0	6.660409434017056E-6	0.0	0.0	0.0
4	0.0	3.9962456604102335E-5	0.0	0.0	0.0
5	6.660409434017056E-6	4.662286603811939E-5	0.0	6.660409434017056E-6	0.0
6	5.3283275472136446E-5	4.662286603811939E-5	0.0	6.660409434017056E-6	0.0
7	5.3283275472136446E-5	4.662286603811939E-5	0.0	6.660409434017056E-6	0.0
8	5.99436849061535E-5	5.99436849061535E-5	0.0	6.660409434017056E-6	0.0
9	6.660409434017055E-5	1.5318941698239227E-4	0.0	3.3302047170085275E-5	0.0
10-11	6.993429905717907E-5	1.5318941698239227E-4	0.0	4.329266132111086E-5	6.660409434017056E-6
12-13	7.32645037741876E-5	1.5318941698239227E-4	0.0	5.3283275472136446E-5	6.660409434017056E-6
14-15	7.992491320820467E-5	2.2312371603957137E-4	0.0	6.993429905717908E-5	6.660409434017056E-6
16-17	9.657593679324731E-5	2.264539207565799E-4	0.0	8.658532264222172E-5	6.660409434017056E-6
18-19	9.990614151025583E-5	2.3311433019059696E-4	0.0	9.324573207623877E-5	6.660409434017056E-6
20-21	1.0989675566128141E-4	2.4310494434162251E-4	0.0	1.0656655094427289E-4	6.660409434017056E-6
22-23	1.1655716509529848E-4	2.830674009457249E-4	0.0	1.3653839339734965E-4	6.660409434017056E-6
24-25	1.19887369812307E-4	3.1636944811581013E-4	0.0	1.6651023585042638E-4	9.990614151025584E-6
26-27	1.2321757452931552E-4	3.3968088113486985E-4	0.0	2.0647269245452872E-4	1.3320818868034112E-5
28-29	1.2654777924632406E-4	3.3968088113486985E-4	0.0	5.095213217023047E-4	1.3320818868034112E-5
30-31	1.2654777924632406E-4	3.6632251887093806E-4	0.0	0.0014619598707667437	1.3320818868034112E-5
32-33	1.2654777924632406E-4	3.6632251887093806E-4	0.0	0.0034933847481419455	1.3320818868034112E-5
34-35	1.2654777924632406E-4	4.529078415131597E-4	0.0	0.0060942746321256056	1.3320818868034112E-5
36-37	1.2654777924632406E-4	5.328327547213644E-4	0.0	0.010440191787821735	1.3320818868034112E-5
38-39	1.2654777924632406E-4	5.394931641553815E-4	0.0	0.01712391265485785	1.3320818868034112E-5
40-41	1.332081886803411E-4	6.127576679295691E-4	0.0	0.02441373078038952	1.3320818868034112E-5
42-43	1.5318941698239227E-4	6.327388962316203E-4	0.0	0.03134388679648426	1.3320818868034112E-5
44-45	1.598498264164093E-4	7.359752424588846E-4	0.0	0.03929641566070063	1.3320818868034112E-5
46-47	1.6651023585042638E-4	7.925887226480296E-4	0.0	0.047641908681524	1.6651023585042638E-5
48-49	1.6651023585042638E-4	7.925887226480296E-4	0.0	0.05627046910329309	1.9981228302051167E-5
50-51	1.8649146415247755E-4	8.891646594412769E-4	0.0	0.06512215324110177	1.9981228302051167E-5
52-53	1.8649146415247755E-4	8.991552735923025E-4	0.0	0.07376070427702189	1.9981228302051167E-5
54-55	1.8649146415247755E-4	9.158062971773451E-4	0.0	0.08236262306105491	1.9981228302051167E-5
56-57	1.8982166886948607E-4	9.457781396304219E-4	0.0	0.08839029359884035	1.9981228302051167E-5
58-59	1.9981228302051165E-4	9.59098958498456E-4	0.0	0.09363869623284579	1.9981228302051167E-5
60-61	2.0980289717153724E-4	9.724197773664901E-4	0.0	0.09864066371779259	1.9981228302051167E-5
62-63	2.1313310188854578E-4	9.857405962345241E-4	0.0	0.10350609280934206	1.9981228302051167E-5
64-65	2.2312371603957137E-4	0.0011122883754808484	0.0	0.1084114843574956	1.9981228302051167E-5
66-67	2.3311433019059693E-4	0.0011122883754808484	0.0	0.11349337675565063	1.9981228302051167E-5
68-69	2.3311433019059693E-4	0.0011222789896318738	0.0	0.11879506266512821	1.9981228302051167E-5
70-71	2.3311433019059693E-4	0.0011289393990658908	0.0	0.12384032281139612	1.9981228302051167E-5
72-73	2.3311433019059693E-4	0.0011322696037828995	0.0	0.12784322888124036	1.9981228302051167E-5
74-75	2.39774739624614E-4	0.0011389300132169165	0.0	0.1309702911105114	1.9981228302051167E-5
76-77	2.4643514905863103E-4	0.0011788924698210189	0.0	0.13374768184449648	1.9981228302051167E-5
78-79	2.497653537756396E-4	0.001205534107557087	0.0	0.13630527906715906	1.9981228302051167E-5
80-81	2.530955584926481E-4	0.001205534107557087	0.0	0.13764069115867947	1.9981228302051167E-5
82-83	2.564257632096566E-4	0.001205534107557087	0.0	0.1381235708426457	1.9981228302051167E-5
84-85	2.664163773606822E-4	0.0012088643122740955	0.0	0.1385564974558568	1.9981228302051167E-5
86-87	2.664163773606822E-4	0.0012188549264251212	0.0	0.1387196774869902	1.9981228302051167E-5
88-89	2.730767867946993E-4	0.0012721382018972575	0.0	0.13880959301434945	1.9981228302051167E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	21805	0.0	51.55115	1
GTATCAA	41130	0.0	39.02298	1
ATCAACG	49700	0.0	31.824146	3
TCAACGC	49955	0.0	31.660326	4
TATCAAC	51455	0.0	31.058113	2
CAACGCA	51365	0.0	30.774492	5
AACGCAG	52840	0.0	29.943497	6
GTGGTAT	10750	0.0	28.042229	1
TGGTATC	10440	0.0	27.176378	2
ACGCAGA	60030	0.0	26.321142	7
CGCAGAG	60930	0.0	25.911064	8
GCAGAGT	69625	0.0	22.687624	9
GAGTACT	44665	0.0	20.634981	12-13
TACATGG	43660	0.0	19.683502	2
CAGAGTA	68585	0.0	19.615353	10-11
GTACATG	44750	0.0	19.513334	1
AGAGTAC	64195	0.0	19.061087	10-11
ACATGGG	43735	0.0	18.868502	3
GTACTTT	49800	0.0	18.512032	14-15
AGTACTT	48245	0.0	17.589447	12-13
>>END_MODULE