FastQCFastQC Report
Fri 27 May 2016
SRR2140169_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140169_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10324630
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127800.050.6346471
GTATCAA228800.035.069171
TCAACGC278900.030.3701324
ATCAACG282050.029.9706083
TATCAAC286700.029.3029772
CAACGCA289300.029.2183955
AACGCAG299150.028.3175726
ACGCAGA354500.023.835057
CGCAGAG363950.023.1923488
GTGGTAT66100.020.078431
GCAGAGT421350.020.0534619
GAGTACT236500.019.95344212-13
TGGTATC64950.018.7644982
CAGAGTA414350.018.32581510-11
GTACTTT260800.017.99467314-15
TATACCG7200.017.4686155
AGAGTAC384550.017.0289710-11
TACATGG295000.016.157922
GTACATG297450.015.9956711
ACATGGG297650.015.7663513
Produced by FastQC (version 0.11.5)