##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140169_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10324630
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.963561212363057	18.0	18.0	32.0	18.0	33.0
2	28.35230860573212	31.0	27.0	32.0	18.0	33.0
3	30.171589877797075	31.0	29.0	33.0	27.0	33.0
4	30.71103458429019	32.0	31.0	33.0	27.0	33.0
5	31.553006354707144	33.0	32.0	33.0	30.0	33.0
6	35.78800024795078	37.0	36.0	38.0	31.0	38.0
7	36.09872576547537	38.0	37.0	38.0	33.0	38.0
8	36.59391658587281	38.0	37.0	38.0	34.0	38.0
9	36.75257389368917	38.0	38.0	38.0	34.0	38.0
10-11	36.82615464186126	38.0	38.0	38.0	35.0	38.0
12-13	36.859670903460945	38.0	38.0	38.0	35.0	38.0
14-15	36.88136790374086	38.0	38.0	38.0	35.0	38.0
16-17	36.8736698554815	38.0	38.0	38.0	35.0	38.0
18-19	36.88104794070102	38.0	38.0	38.0	35.0	38.0
20-21	36.88202303813398	38.0	38.0	38.0	35.0	38.0
22-23	36.89116674645385	38.0	38.0	38.0	35.0	38.0
24-25	36.88994323072248	38.0	38.0	38.0	35.0	38.0
26-27	36.842054515159425	38.0	38.0	38.0	35.0	38.0
28-29	36.82176999326738	38.0	38.0	38.0	35.0	38.0
30-31	36.8131821246798	38.0	38.0	38.0	35.0	38.0
32-33	36.794910598083405	38.0	38.0	38.0	35.0	38.0
34-35	36.76702678997644	38.0	38.0	38.0	35.0	38.0
36-37	36.74272611690576	38.0	38.0	38.0	35.0	38.0
38-39	36.73117933738291	38.0	38.0	38.0	34.5	38.0
40-41	36.720465914204354	38.0	38.0	38.0	34.5	38.0
42-43	36.67637143656529	38.0	38.0	38.0	34.0	38.0
44-45	36.615055146518515	38.0	38.0	38.0	34.0	38.0
46-47	36.57234045373784	38.0	38.0	38.0	34.0	38.0
48-49	36.541308088213306	38.0	38.0	38.0	34.0	38.0
50-51	36.52901981609028	38.0	38.0	38.0	34.0	38.0
52-53	36.492971189754	38.0	38.0	38.0	34.0	38.0
54-55	36.464515876568115	38.0	38.0	38.0	34.0	38.0
56-57	36.43698743791144	38.0	38.0	38.0	34.0	38.0
58-59	36.41522111155217	38.0	38.0	38.0	34.0	38.0
60-61	36.41478405580144	38.0	38.0	38.0	34.0	38.0
62-63	36.39990539010479	38.0	38.0	38.0	34.0	38.0
64-65	36.393406364417594	38.0	38.0	38.0	34.0	38.0
66-67	36.3664319009931	38.0	38.0	38.0	34.0	38.0
68-69	36.36306027827018	38.0	38.0	38.0	34.0	38.0
70-71	36.350595880799645	38.0	38.0	38.0	34.0	38.0
72-73	36.332013467029114	38.0	38.0	38.0	33.0	38.0
74-75	36.32374114656286	38.0	38.0	38.0	33.0	38.0
76-77	36.29975051945274	38.0	37.0	38.0	33.0	38.0
78-79	36.310161680907484	38.0	37.0	38.0	33.0	38.0
80-81	36.270275985087295	38.0	37.0	38.0	33.0	38.0
82-83	36.26821741627729	38.0	37.0	38.0	33.0	38.0
84-85	36.254419343109916	38.0	37.0	38.0	33.0	38.0
86-87	36.21927322197125	38.0	37.0	38.0	33.0	38.0
88-89	36.21040396949731	38.0	37.0	38.0	33.0	38.0
90-91	36.213842701624216	38.0	37.0	38.0	33.0	38.0
92-93	36.194684285760566	38.0	37.0	38.0	33.0	38.0
94-95	36.17254993750949	38.0	37.0	38.0	33.0	38.0
96-97	36.176629460478054	38.0	37.0	38.0	33.0	38.0
98-99	36.21002752760445	38.0	37.0	38.0	33.0	38.0
100-101	35.626047577088045	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	6.0
18	24.0
19	50.0
20	110.0
21	475.0
22	2019.0
23	6118.0
24	14408.0
25	27210.0
26	45501.0
27	69626.0
28	99627.0
29	134094.0
30	172759.0
31	220714.0
32	281493.0
33	372225.0
34	523862.0
35	844940.0
36	2009777.0
37	5499592.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.610132275926595	24.823204318217698	14.169650631548055	23.39701277430765
2	14.627168237505847	20.749779895260172	39.435863561212365	25.187188306021618
3	18.00608587994877	25.17942749412886	31.170162295943545	25.64432432997883
4	13.787041278961087	18.007889871114024	38.0779456503526	30.127123199572285
5	14.195153566825097	36.548585593832	34.86479156632144	14.391469273021459
6	30.38080783524446	37.928923360934	18.13465470433323	13.555614099488311
7	26.68611853402979	32.74366248475732	22.742325875116105	17.82789310609678
8	24.248433115762985	37.30751610469334	20.46895627252502	17.975094507018653
9	25.169880179725567	17.279437616650668	21.41948912454974	36.13119307907402
10-11	24.37871251251316	26.96469417761617	28.323798769127624	20.33279454074304
12-13	24.98706005394098	24.29816289571198	28.769262940285056	21.94551411006199
14-15	22.188589052449245	25.708647739303757	26.408934403357254	25.693828804889744
16-17	21.802011306942717	28.81302768234794	27.185589217240715	22.19937179346863
18-19	21.832884083981703	27.964561441911233	28.878628096115794	21.32392637799127
20-21	22.688888427202762	27.041599066373788	28.83519156350282	21.43432094292063
22-23	22.3655089613876	26.871638601842402	28.727762406389374	22.035090030380623
24-25	22.08539604260115	27.067497029747088	28.842451392228856	22.0046555354229
26-27	22.07456838136357	27.178302807127565	28.72853411577974	22.018594695729128
28-29	22.050681381990792	27.2255221240842	28.768444772639022	21.95535172128598
30-31	22.286555450323757	27.079656309762747	28.698602339818862	21.93518590009463
32-33	22.099537062454736	27.207956333973083	28.628238150519756	22.064268453052424
34-35	22.18496973236552	27.249939293300972	28.614707706375746	21.95038326795776
36-37	22.25033893605627	27.115563864526454	28.61508374075144	22.01901345866583
38-39	22.040395927594584	27.27528722989688	28.682143384822538	22.002173457686
40-41	22.345771683928746	27.171046552023192	28.500874922848162	21.982306841199897
42-43	22.19486736740507	27.265318838856768	28.328603122888563	22.2112106708496
44-45	22.225676550296097	27.320413723448944	28.25617717658225	22.197732549672715
46-47	22.306512265046162	27.28199520015003	28.07948589525877	22.33200663954504
48-49	22.262031202010053	27.406813036437566	28.061871190135907	22.269284571416474
50-51	22.24378311384202	27.470919721784494	27.961701825177688	22.323595339195805
52-53	22.41526240810689	27.441921570842997	27.818717280258277	22.32409874079184
54-55	22.4007759910966	27.317723487785166	27.897367464134533	22.3841330569837
56-57	22.351576554556633	27.412931032136544	27.917150886187148	22.318341527119678
58-59	22.29640809373639	27.422528643730598	27.956986356624157	22.324076905908854
60-61	22.352289728571535	27.362575082659795	27.99955667539059	22.28557851337808
62-63	22.351021973728155	27.460216005515946	27.97051487550305	22.218247145252846
64-65	22.244832162472132	27.4243274911231	27.97204178246452	22.358798563940244
66-67	22.29838247165783	27.5535811568624	27.76907349016069	22.37896288131908
68-69	22.300636179039195	27.461915168442797	27.836722280868464	22.400726371649547
70-71	22.265349675563552	27.251575758593066	27.858772630902624	22.624301934940753
72-73	22.348695898700125	27.30231664841472	27.775365549474557	22.573621903410604
74-75	22.191903078467416	27.374322999046917	27.81082282554186	22.6229510969438
76-77	22.403165380708	27.35987653067256	27.67670247507006	22.56025561354938
78-79	22.369147698714727	27.42759819056509	27.584611186903135	22.618642923817045
80-81	22.412712823857472	27.378132382912973	27.569071675543494	22.640083117686057
82-83	22.408398754840537	27.409923674322208	27.655639658596197	22.52603791224106
84-85	22.540086694757775	27.426748740654883	27.568082037220886	22.465082527366462
86-87	22.436285146755427	27.450598515315573	27.5689348717976	22.544181466131402
88-89	22.442185896230992	27.465670078560773	27.58430011448792	22.507843910720315
90-91	22.477983663859664	27.547396207131484	27.57060920602022	22.404010922988633
92-93	22.577666161818975	27.49484525774205	27.535736316185343	22.391752264253636
94-95	22.454629584834755	27.58352697723559	27.598034273800252	22.363809164129396
96-97	22.426380466648943	27.605045088620194	27.6085740602121	22.36000038451877
98-99	22.717406866946234	28.097419419157614	27.715682292600757	21.469491421295395
100-101	23.280988554440224	30.62915556881115	25.02805941664839	21.061796460100236
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2532.0
1	2434.0
2	2674.5
3	3154.0
4	3756.5
5	4925.0
6	6431.5
7	8571.5
8	10721.5
9	12146.0
10	12215.5
11	11874.5
12	12981.5
13	16116.5
14	22898.0
15	31002.5
16	36811.5
17	39792.5
18	39778.0
19	37348.5
20	34127.5
21	31691.0
22	31939.5
23	36804.5
24	46130.0
25	60325.5
26	78864.0
27	100086.5
28	122096.0
29	144637.5
30	165799.5
31	187354.5
32	210424.0
33	234135.5
34	257364.0
35	278886.5
36	305233.5
37	327771.5
38	342479.5
39	356285.5
40	372169.5
41	386607.0
42	394909.0
43	402088.5
44	410231.5
45	417710.0
46	422751.0
47	425638.5
48	424538.5
49	421328.5
50	417655.0
51	408601.5
52	391240.0
53	369974.0
54	350421.5
55	328427.5
56	303015.5
57	275844.0
58	247055.5
59	216364.0
60	182643.5
61	150070.0
62	119342.5
63	92280.0
64	71080.5
65	55040.5
66	42975.5
67	32085.0
68	23383.5
69	17759.0
70	13110.0
71	9138.0
72	6378.0
73	4328.0
74	2807.0
75	1742.5
76	1076.0
77	686.5
78	450.5
79	269.5
80	162.0
81	105.5
82	72.0
83	48.5
84	35.0
85	25.5
86	18.0
87	15.0
88	12.5
89	9.0
90	6.0
91	3.0
92	2.0
93	2.0
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.003912973152548808
4	0.0
5	0.038829478635069734
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.452836566540399E-5
12-13	1.1622692532323192E-4
14-15	9.685577110269325E-6
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	5.007882908486249E-5
46-47	9.240299906809303E-4
48-49	2.545041249265056E-5
50-51	1.1283041875266177E-4
52-53	0.0014356276936093703
54-55	8.160272746461418E-4
56-57	0.003946176662478695
58-59	0.008681001651604047
60-61	0.010816290459686502
62-63	0.006140706335415445
64-65	0.007904340751004093
66-67	0.005397491515818024
68-69	0.002623915795797431
70-71	0.004287632324559807
72-73	0.00284503178917789
74-75	0.0014038067631237893
76-77	0.001894468221063619
78-79	6.778154673813369E-4
80-81	5.22180759135944E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	1.9751113746787512E-4
94-95	2.809064163395147E-4
96-97	1.0751335584663003E-4
98-99	5.549422899190881E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	94.0
20-21	356.0
22-23	772.0
24-25	1112.0
26-27	2649.0
28-29	6026.0
30-31	17357.0
32-33	22743.0
34-35	31620.0
36-37	50615.0
38-39	58415.0
40-41	60662.0
42-43	68159.0
44-45	81004.0
46-47	80748.0
48-49	75419.0
50-51	69519.0
52-53	61445.0
54-55	65492.0
56-57	65900.0
58-59	74120.0
60-61	73550.0
62-63	71149.0
64-65	76173.0
66-67	80567.0
68-69	82356.0
70-71	81749.0
72-73	76958.0
74-75	78578.0
76-77	83463.0
78-79	86172.0
80-81	87226.0
82-83	82524.0
84-85	82084.0
86-87	85133.0
88-89	88507.0
90-91	91005.0
92-93	90099.0
94-95	97318.0
96-97	156803.0
98-99	878167.0
100-101	6900822.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.75264656158168
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.1165173532748	74.63744180687884
2	7.4500406510781465	12.479212430383276
3	1.8644827707352996	4.684660995526562
4	0.6853661070973319	2.296049013320399
5	0.3368502551059732	1.41060501800346
6	0.1737552789355136	0.8731478678937064
7	0.10729962512946205	0.6290639305760628
8	0.06704068441079365	0.44918677973629917
9	0.04249874483043845	0.32034441195851016
>10	0.1529208117593635	1.9396101562586765
>50	0.002681716967958877	0.1494608584270065
>100	4.8597044858346227E-4	0.07231015257480602
>500	2.001156483832105E-5	0.008949954901036736
>1k	4.001866142940017E-5	0.049956623561387054
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.874230844107731E-5	0.0	1.9371154220538654E-5	0.0
3	0.0	3.874230844107731E-5	0.0	1.9371154220538654E-5	0.0
4	0.0	7.748461688215462E-5	0.0	1.9371154220538654E-5	0.0
5	0.0	8.717019399242394E-5	0.0	1.9371154220538654E-5	0.0
6	7.748461688215462E-5	9.685577110269327E-5	0.0	1.9371154220538654E-5	0.0
7	7.748461688215462E-5	9.685577110269327E-5	0.0	1.9371154220538654E-5	0.0
8	7.748461688215462E-5	9.685577110269327E-5	0.0	1.9371154220538654E-5	0.0
9	7.748461688215462E-5	2.0339711931565587E-4	0.0	1.9371154220538654E-5	0.0
10-11	8.232740543728928E-5	2.130826964259252E-4	9.685577110269327E-6	2.905673133080798E-5	0.0
12-13	8.717019399242394E-5	2.372966392015985E-4	9.685577110269327E-6	2.905673133080798E-5	0.0
14-15	8.717019399242394E-5	3.0993846752861847E-4	9.685577110269327E-6	3.874230844107731E-5	0.0
16-17	1.1622692532323192E-4	3.341524103042918E-4	9.685577110269327E-6	3.874230844107731E-5	0.0
18-19	1.1622692532323192E-4	3.486807759696958E-4	9.685577110269327E-6	3.874230844107731E-5	0.0
20-21	1.2591250243350124E-4	4.0195145007617707E-4	9.685577110269327E-6	4.3585096996211976E-5	0.0
22-23	1.2591250243350124E-4	4.7943606695833164E-4	9.685577110269327E-6	4.842788555134664E-5	0.0
24-25	1.2591250243350124E-4	5.375495296199476E-4	9.685577110269327E-6	8.717019399242395E-5	0.0
26-27	1.2591250243350124E-4	5.61763472395621E-4	9.685577110269327E-6	1.452836566540399E-4	0.0
28-29	1.2591250243350124E-4	5.61763472395621E-4	9.685577110269327E-6	4.939644326237357E-4	0.0
30-31	1.2591250243350124E-4	6.876759748291222E-4	9.685577110269327E-6	0.0016804476286317281	0.0
32-33	1.307552909886359E-4	6.973615519393915E-4	9.685577110269327E-6	0.0045522212418265835	0.0
34-35	1.3559807954377056E-4	7.942173230420848E-4	9.685577110269327E-6	0.008334439103386755	0.0
36-37	1.3559807954377056E-4	8.862303055896434E-4	9.685577110269327E-6	0.013869746421905676	0.0
38-39	1.3559807954377056E-4	9.104442483653166E-4	9.685577110269327E-6	0.024218785564228452	0.0
40-41	1.3559807954377056E-4	0.0010557279050193566	9.685577110269327E-6	0.05812799102728136	0.0
42-43	1.3559807954377056E-4	0.00107994184779503	9.685577110269327E-6	0.06853030084371062	0.0
44-45	1.3559807954377056E-4	0.0012155399273388004	9.685577110269327E-6	0.0831409939145519	0.0
46-47	1.3559807954377056E-4	0.0013269240641068977	9.685577110269327E-6	0.10010528222318862	0.0
48-49	1.3559807954377056E-4	0.001336609641217167	9.685577110269327E-6	0.11601868541536113	0.0
50-51	1.5012644520917459E-4	0.0014431509894301297	9.685577110269327E-6	0.13170932033399746	0.0
52-53	1.5496923376430923E-4	0.0014528365665403991	9.685577110269327E-6	0.16092586368712486	0.0
54-55	1.5496923376430923E-4	0.001467364932205803	9.685577110269327E-6	0.23293812950197731	0.0
56-57	1.5496923376430923E-4	0.0014722077207609376	9.685577110269327E-6	0.3126068440224976	0.0
58-59	1.5496923376430923E-4	0.0014867360864263416	9.685577110269327E-6	0.3989828206918795	0.0
60-61	1.5496923376430923E-4	0.0015496923376430924	9.685577110269327E-6	0.41954045810842616	0.0
62-63	1.7434038798484788E-4	0.0015835918575290348	9.685577110269327E-6	0.43285328384649135	0.0
64-65	1.7434038798484788E-4	0.0016804476286317281	9.685577110269327E-6	0.44299408308094335	0.0
66-67	1.7434038798484788E-4	0.00172403272562794	9.685577110269327E-6	0.4497933582123524	0.0
68-69	1.7434038798484788E-4	0.0017385610912933442	9.685577110269327E-6	0.45630690881900854	0.0
70-71	1.7918317653998252E-4	0.0017627750340690174	9.685577110269327E-6	0.4626461190376798	0.0
72-73	1.9371154220538655E-4	0.0017918317653998254	9.685577110269327E-6	0.4669465152746394	0.0
74-75	1.9371154220538655E-4	0.0018596308051717107	9.685577110269327E-6	0.47051080765121844	0.0
76-77	2.130826964259252E-4	0.0019758577304949424	9.685577110269327E-6	0.47393950194825385	0.0
78-79	2.130826964259252E-4	0.00201460003893602	9.685577110269327E-6	0.476893602966886	0.0
80-81	2.130826964259252E-4	0.00201460003893602	9.685577110269327E-6	0.47830285443643017	0.0
82-83	2.130826964259252E-4	0.0020388139817116933	9.685577110269327E-6	0.4789324169485977	0.0
84-85	2.130826964259252E-4	0.0020630279244873667	9.685577110269327E-6	0.47944575253544197	0.0
86-87	2.2276827353619451E-4	0.002116298598593848	9.685577110269327E-6	0.47955229388365495	0.0
88-89	2.3245385064646384E-4	0.0022373683124722148	9.685577110269327E-6	0.47958135061498575	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12780	0.0	50.634647	1
GTATCAA	22880	0.0	35.06917	1
TCAACGC	27890	0.0	30.370132	4
ATCAACG	28205	0.0	29.970608	3
TATCAAC	28670	0.0	29.302977	2
CAACGCA	28930	0.0	29.218395	5
AACGCAG	29915	0.0	28.317572	6
ACGCAGA	35450	0.0	23.83505	7
CGCAGAG	36395	0.0	23.192348	8
GTGGTAT	6610	0.0	20.07843	1
GCAGAGT	42135	0.0	20.053461	9
GAGTACT	23650	0.0	19.953442	12-13
TGGTATC	6495	0.0	18.764498	2
CAGAGTA	41435	0.0	18.325815	10-11
GTACTTT	26080	0.0	17.994673	14-15
TATACCG	720	0.0	17.468615	5
AGAGTAC	38455	0.0	17.02897	10-11
TACATGG	29500	0.0	16.15792	2
GTACATG	29745	0.0	15.995671	1
ACATGGG	29765	0.0	15.766351	3
>>END_MODULE