FastQCFastQC Report
Fri 27 May 2016
SRR2140168_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140168_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8399720
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126600.052.05021
GTATCAA228800.034.6669241
ATCAACG277900.029.6673133
TCAACGC279850.029.5216224
TATCAAC282400.029.1022782
CAACGCA288500.028.5913165
AACGCAG300900.027.4595366
TGGTATC55200.025.653642
GTGGTAT58600.024.0169541
ACGCAGA355250.023.270847
CGCAGAG361650.022.8590248
GAGTACT208500.020.56412512-13
GCAGAGT417650.019.8667769
CAGAGTA408300.018.72129210-11
GTACTTT232600.018.32144514-15
AGAGTAC382300.016.81447610-11
GTACATG317350.016.3705481
GGACCGA14100.016.3204966
TACATGG319000.016.1769372
CTTATAC77700.016.0446931
Produced by FastQC (version 0.11.5)