##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140168_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8399720
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.688831056273305	18.0	18.0	32.0	18.0	33.0
2	27.99910889886806	30.0	25.0	32.0	18.0	33.0
3	29.979105493992655	31.0	29.0	33.0	25.0	33.0
4	30.593689432504892	32.0	31.0	33.0	27.0	33.0
5	31.466532098689004	33.0	32.0	33.0	28.0	33.0
6	35.72710257008567	37.0	36.0	38.0	31.0	38.0
7	36.053640597543726	38.0	37.0	38.0	33.0	38.0
8	36.56469096588934	38.0	37.0	38.0	34.0	38.0
9	36.727041972827664	38.0	38.0	38.0	34.0	38.0
10-11	36.80124414861448	38.0	38.0	38.0	34.5	38.0
12-13	36.829269963760694	38.0	38.0	38.0	35.0	38.0
14-15	36.85355380893649	38.0	38.0	38.0	35.0	38.0
16-17	36.842767377960215	38.0	38.0	38.0	35.0	38.0
18-19	36.852333649216874	38.0	38.0	38.0	35.0	38.0
20-21	36.85481894075818	38.0	38.0	38.0	35.0	38.0
22-23	36.860483946449094	38.0	38.0	38.0	35.0	38.0
24-25	36.854448153817046	38.0	38.0	38.0	35.0	38.0
26-27	36.8070335905967	38.0	38.0	38.0	35.0	38.0
28-29	36.78765587055547	38.0	38.0	38.0	35.0	38.0
30-31	36.77991382932774	38.0	38.0	38.0	35.0	38.0
32-33	36.759552251183166	38.0	38.0	38.0	35.0	38.0
34-35	36.726471353135395	38.0	38.0	38.0	34.5	38.0
36-37	36.7024737483684	38.0	38.0	38.0	34.0	38.0
38-39	36.67953272888955	38.0	38.0	38.0	34.0	38.0
40-41	36.68037393460607	38.0	38.0	38.0	34.0	38.0
42-43	36.637297535889	38.0	38.0	38.0	34.0	38.0
44-45	36.56757665720268	38.0	38.0	38.0	34.0	38.0
46-47	36.5149499481995	38.0	38.0	38.0	34.0	38.0
48-49	36.48062090887626	38.0	38.0	38.0	34.0	38.0
50-51	36.46434489137457	38.0	38.0	38.0	34.0	38.0
52-53	36.42502178511694	38.0	38.0	38.0	34.0	38.0
54-55	36.39255334453133	38.0	38.0	38.0	34.0	38.0
56-57	36.36249367587159	38.0	38.0	38.0	34.0	38.0
58-59	36.341045394351355	38.0	38.0	38.0	33.5	38.0
60-61	36.340111295652406	38.0	38.0	38.0	33.5	38.0
62-63	36.32361831564581	38.0	38.0	38.0	33.0	38.0
64-65	36.313545049955714	38.0	38.0	38.0	33.0	38.0
66-67	36.28284922870979	38.0	37.0	38.0	33.0	38.0
68-69	36.27610636337478	38.0	37.0	38.0	33.0	38.0
70-71	36.260845822171916	38.0	37.0	38.0	33.0	38.0
72-73	36.239457811482154	38.0	37.0	38.0	33.0	38.0
74-75	36.23235158647131	38.0	37.0	38.0	33.0	38.0
76-77	36.21221548091438	38.0	37.0	38.0	33.0	38.0
78-79	36.22319571798444	38.0	37.0	38.0	33.0	38.0
80-81	36.17898991340601	38.0	37.0	38.0	33.0	38.0
82-83	36.18475022217331	38.0	37.0	38.0	33.0	38.0
84-85	36.16953564449258	38.0	37.0	38.0	33.0	38.0
86-87	36.13401183941343	38.0	37.0	38.0	33.0	38.0
88-89	36.12951945697537	38.0	37.0	38.0	33.0	38.0
90-91	36.13073116782492	38.0	37.0	38.0	33.0	38.0
92-93	36.11421261895798	38.0	37.0	38.0	33.0	38.0
94-95	36.09284035717546	38.0	37.0	38.0	33.0	38.0
96-97	36.10109143330038	38.0	37.0	38.0	33.0	38.0
98-99	36.13505132042304	38.0	37.0	38.0	33.0	38.0
100-101	35.53696592682761	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	17.0
18	44.0
19	94.0
20	163.0
21	522.0
22	2013.0
23	5839.0
24	12874.0
25	24059.0
26	39517.0
27	59031.0
28	84427.0
29	114382.0
30	147276.0
31	187044.0
32	238100.0
33	313204.0
34	439357.0
35	713860.0
36	1706734.0
37	4311163.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	37.28679051206469	25.51618387279576	14.29866709842709	22.89835851671246
2	14.620784978546904	20.623794602677233	39.63802364840733	25.11739677036854
3	18.38786310719466	25.145664334132434	30.978392865816893	25.488079692856008
4	13.801983875652999	18.141771392379745	37.59356264256428	30.462682089402982
5	14.201976300166294	36.70543275914451	34.505875533653565	14.586715407035634
6	30.310879410266057	37.81996304638726	17.99074254856114	13.87841499478554
7	26.87214573819127	32.87252432223931	22.094665060263914	18.160664879305504
8	24.282237979361216	37.7743424780826	19.98879724562247	17.954622296933707
9	24.998416613887127	17.607265480277913	21.125882767520825	36.26843513831414
10-11	24.545447564701124	27.213745317774634	27.702821231449605	20.537985886074633
12-13	25.231915717100268	24.421119964722596	28.5238623090723	21.823102009104836
14-15	22.33636359307215	25.796681317948693	26.193188582476555	25.6737665065026
16-17	21.715408370755217	29.20341987590063	26.937195525565137	22.14397622777902
18-19	21.7475642045211	28.046089631559145	28.942345697237528	21.264000466682223
20-21	22.639164515853672	26.913798939155814	29.001771169053725	21.445265375936792
22-23	22.3857399755783	26.92496494857537	28.852656383645463	21.836638692200868
24-25	22.061411055180645	27.181699922305935	28.989078179279797	21.76781084323362
26-27	21.945403028613228	27.24322241875224	28.95158464358582	21.859789909048715
28-29	21.758180490956033	27.175047570430895	29.036133275237198	22.030638663375875
30-31	21.972314522779225	27.07892067250024	29.080201963740386	21.868562840980143
32-33	21.83131189510558	27.189304165046014	29.006296357409937	21.97308758243847
34-35	21.913515441789976	27.309959861760696	29.065855203067407	21.710669493381918
36-37	21.99982294808968	27.18869913931631	28.870818409843224	21.94065950275079
38-39	21.90685091353785	27.230885731412574	28.95996453951876	21.902298815530816
40-41	22.063693735334287	27.09188542326758	28.935475354314892	21.908945487083244
42-43	22.054457380914567	27.18116623703468	28.762837037127532	22.001539344923216
44-45	21.97990735585505	27.288014681235946	28.672891047136705	22.059186915772298
46-47	22.154249893186044	27.292072451177184	28.338991837491484	22.214685818145288
48-49	22.124836341446354	27.368560469144	28.286979738935113	22.219623450474533
50-51	22.045412123350975	27.480834884402228	28.26052566814355	22.213227324103247
52-53	22.145523857152657	27.454691805882476	28.255427048440623	22.14435728852425
54-55	22.100767949462067	27.477517972128005	28.174952596125753	22.246761482284167
56-57	22.201175431092686	27.57023568879946	28.067030896212568	22.161557983895285
58-59	22.02676496091078	27.667894352665968	28.2500563852202	22.055284301203056
60-61	22.192152800218455	27.725711057609924	28.110199747899355	21.97193639427226
62-63	22.082412571935357	27.878713573378157	28.03077923599357	22.00809461869292
64-65	22.03512623301704	27.857059573540855	27.996750908526675	22.111063284915435
66-67	22.099077441382768	27.825502288183362	27.95237688539825	22.123043385035622
68-69	22.088175061908917	27.653551867126524	28.016490714184965	22.241782356779595
70-71	22.05211358458829	27.484867001080538	28.112484996284792	22.350534418046372
72-73	22.089431375573295	27.504962603130327	28.057918759855333	22.347687261441042
74-75	21.997185247361898	27.526544427273024	28.066703323438034	22.40956700192704
76-77	22.175528151853243	27.462080957821865	27.997452559826858	22.364938330498035
78-79	22.084639771221084	27.49215996345039	27.972345687780848	22.45085457754768
80-81	22.131753987551207	27.555005747469057	27.934976127265855	22.37826413771388
82-83	22.164968410122583	27.500945803637876	28.011518153647643	22.322567632591902
84-85	22.23696396892219	27.601319826922776	27.905715712366113	22.25600049178892
86-87	22.22387561456264	27.60189999653958	27.899744726464892	22.274479662432885
88-89	22.211628743638556	27.549404837097562	27.895391984563368	22.343574434700514
90-91	22.222660974564167	27.55892869019562	27.92483570238448	22.29357463285573
92-93	22.206963648323256	27.510343670981523	27.961223087651515	22.321469593043698
94-95	22.105355616971373	27.622325716014988	28.003429268627794	22.268889398385845
96-97	22.119014771876675	27.675398765647643	27.937043738296058	22.26854272417963
98-99	22.393395135146797	28.217606759277853	27.995681848409575	21.39331625716578
100-101	23.011267459111544	30.70085694042568	25.36238136094659	20.92549423951619
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3368.0
1	3045.5
2	2690.5
3	2742.5
4	3154.5
5	4095.5
6	5586.0
7	8194.0
8	10771.0
9	12264.0
10	12178.0
11	11475.0
12	12340.0
13	15325.0
14	21675.5
15	29236.5
16	34338.5
17	36520.5
18	36257.0
19	33855.5
20	30487.0
21	27744.0
22	27259.5
23	30349.0
24	37218.0
25	47909.0
26	61414.0
27	76123.5
28	93193.5
29	112207.0
30	131161.0
31	151840.0
32	171436.0
33	190137.0
34	208749.0
35	225562.5
36	243893.5
37	261579.5
38	277321.5
39	290893.5
40	302310.5
41	313866.0
42	323069.0
43	331818.0
44	346325.5
45	357661.0
46	359300.5
47	358099.5
48	353780.0
49	346372.5
50	339139.5
51	331135.5
52	316624.0
53	297726.5
54	280438.5
55	261827.0
56	240914.5
57	216709.5
58	191072.5
59	165343.5
60	138993.0
61	114520.0
62	91600.0
63	71503.5
64	54775.5
65	41123.0
66	31030.0
67	23048.0
68	16604.5
69	12001.0
70	8534.5
71	5847.0
72	4055.0
73	2766.0
74	1821.5
75	1201.0
76	760.5
77	464.5
78	270.0
79	157.0
80	100.5
81	64.5
82	45.0
83	33.0
84	22.5
85	13.0
86	8.5
87	7.0
88	5.0
89	3.0
90	1.5
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.003238203178201178
4	0.0
5	0.03715600043810984
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.7857738115080028E-5
12-13	1.0714642869048015E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	3.690853382510264E-5
46-47	9.301564151027637E-4
48-49	6.250385961333112E-6
50-51	7.556968204811976E-5
52-53	0.0013504117519551014
54-55	7.209662734529371E-4
56-57	0.0037322649162922356
58-59	0.009128202334788433
60-61	0.011278836170145351
62-63	0.006497717257976458
64-65	0.008361712176758252
66-67	0.005488694457934332
68-69	0.0027224558060851335
70-71	0.004769661090744605
72-73	0.003076424003193987
74-75	0.0013292633873092732
76-77	0.002094660793423994
78-79	7.821127020175972E-4
80-81	3.9838715804687284E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	7.46459505243654E-6
92-93	2.187814686965966E-4
94-95	2.821117513268783E-4
96-97	1.1577539295905002E-4
98-99	5.014417644637296E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	111.0
20-21	356.0
22-23	743.0
24-25	1032.0
26-27	2286.0
28-29	5118.0
30-31	13746.0
32-33	17334.0
34-35	24498.0
36-37	40272.0
38-39	48171.0
40-41	48037.0
42-43	54201.0
44-45	64278.0
46-47	64327.0
48-49	61480.0
50-51	55139.0
52-53	49017.0
54-55	52418.0
56-57	54013.0
58-59	58404.0
60-61	60023.0
62-63	57571.0
64-65	62967.0
66-67	67844.0
68-69	67773.0
70-71	72393.0
72-73	59502.0
74-75	60067.0
76-77	63646.0
78-79	67519.0
80-81	68082.0
82-83	64457.0
84-85	62665.0
86-87	64928.0
88-89	69301.0
90-91	71140.0
92-93	69364.0
94-95	74336.0
96-97	122887.0
98-99	711875.0
100-101	5666399.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.10646673346561
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.76113548906791	74.65385489235923
2	7.8459136721962945	13.197841545284414
3	1.901018975241325	4.7966396760246415
4	0.6990861733180819	2.3519067192001213
5	0.327415166279515	1.3768866395360069
6	0.16460564223345278	0.8306639383589184
7	0.09134209874352693	0.5377722832536165
8	0.05780102068656962	0.38891516988282604
9	0.03515083707581883	0.2660771438253743
>10	0.11411607040744695	1.439175442007823
>50	0.002065340437816051	0.10922514940888087
>100	3.495143121910149E-4	0.05104140085815062
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.1905158743386685E-5	0.0	1.1905158743386685E-5	0.0
5	0.0	1.1905158743386685E-5	0.0	1.1905158743386685E-5	0.0
6	0.0	1.1905158743386685E-5	0.0	1.1905158743386685E-5	0.0
7	0.0	1.1905158743386685E-5	0.0	1.1905158743386685E-5	0.0
8	0.0	1.1905158743386685E-5	0.0	1.1905158743386685E-5	1.1905158743386685E-5
9	1.1905158743386685E-5	7.143095246032011E-5	0.0	3.5715476230160056E-5	1.1905158743386685E-5
10-11	1.1905158743386685E-5	7.143095246032011E-5	0.0	3.5715476230160056E-5	1.1905158743386685E-5
12-13	1.1905158743386685E-5	7.143095246032011E-5	0.0	3.5715476230160056E-5	1.1905158743386685E-5
14-15	1.1905158743386685E-5	1.3690932554894688E-4	0.0	3.5715476230160056E-5	1.1905158743386685E-5
16-17	1.7857738115080028E-5	1.4286190492064022E-4	0.0	3.5715476230160056E-5	1.1905158743386685E-5
18-19	2.381031748677337E-5	1.7857738115080026E-4	0.0	4.16680556018534E-5	2.381031748677337E-5
20-21	2.381031748677337E-5	2.0834027800926698E-4	0.0	5.357321434524008E-5	2.381031748677337E-5
22-23	3.5715476230160056E-5	2.5000833361112036E-4	0.0	7.143095246032011E-5	2.9762896858466715E-5
24-25	4.762063497354674E-5	3.1548670669974714E-4	0.0	9.524126994709348E-5	4.762063497354674E-5
26-27	5.952579371693342E-5	3.333444448148272E-4	0.0	1.428619049206402E-4	4.762063497354674E-5
28-29	7.143095246032011E-5	3.333444448148272E-4	0.0	4.881115084788541E-4	4.762063497354674E-5
30-31	7.143095246032011E-5	3.9287023853176056E-4	0.0	0.0016786273828175227	5.357321434524008E-5
32-33	7.143095246032011E-5	4.1668055601853395E-4	0.0	0.004160852980813646	5.952579371693342E-5
34-35	7.143095246032011E-5	4.5239603224869404E-4	0.0	0.007678827389484412	5.952579371693342E-5
36-37	7.143095246032011E-5	4.762063497354674E-4	0.0	0.01312543751458382	5.952579371693342E-5
38-39	7.143095246032011E-5	4.881115084788541E-4	0.0	0.022893620263532595	6.547837308862676E-5
40-41	7.143095246032011E-5	5.476373021957875E-4	0.0	0.047799212354697536	7.143095246032011E-5
42-43	7.143095246032011E-5	5.654950403108676E-4	0.0	0.057341197087521965	7.738353183201345E-5
44-45	7.143095246032011E-5	6.42878572142881E-4	0.0	0.06953207964074992	8.33361112037068E-5
46-47	7.143095246032011E-5	7.024043658598143E-4	0.0	0.08372898144223856	8.33361112037068E-5
48-49	8.928869057540014E-5	7.083569452315077E-4	0.0	0.09657464772635278	8.33361112037068E-5
50-51	1.0714642869048016E-4	7.916930564352145E-4	0.0	0.10973580071716676	8.33361112037068E-5
52-53	1.0714642869048016E-4	8.035982151786012E-4	0.0	0.12958765292176405	8.928869057540014E-5
54-55	1.250041668055602E-4	8.095507945502945E-4	0.0	0.17135690237293624	9.524126994709348E-5
56-57	1.4881448429233355E-4	8.214559532936812E-4	0.0	0.21847156809988905	9.524126994709348E-5
58-59	1.6071964303572024E-4	8.214559532936812E-4	0.0	0.27058044791969255	9.524126994709348E-5
60-61	1.666722224074136E-4	8.750291676389213E-4	0.0	0.28956917611539434	9.524126994709348E-5
62-63	1.666722224074136E-4	8.869343263823079E-4	0.0	0.29985523326968044	1.0714642869048016E-4
64-65	1.666722224074136E-4	9.643178582143215E-4	0.0	0.3093912654231331	1.0714642869048016E-4
66-67	1.666722224074136E-4	9.821755963294015E-4	0.0	0.3150700261437286	1.0714642869048016E-4
68-69	1.666722224074136E-4	0.0010000333344444814	0.0	0.32032020114956217	1.0714642869048016E-4
70-71	1.666722224074136E-4	0.0010119384931878681	0.0	0.3260584876638745	1.0714642869048016E-4
72-73	1.7262480177910694E-4	0.001029796231302948	0.0	0.33004671584290907	1.3095674617725353E-4
74-75	1.7857738115080026E-4	0.001041701390046335	0.0	0.3333027767592253	1.3095674617725353E-4
76-77	1.7857738115080026E-4	0.0010536065487897215	0.0	0.3362373983894701	1.3095674617725353E-4
78-79	1.7857738115080026E-4	0.001059559128161415	0.0	0.3389220116861038	1.3095674617725353E-4
80-81	1.7857738115080026E-4	0.001059559128161415	0.0	0.34011848013981416	1.4881448429233355E-4
82-83	1.7857738115080026E-4	0.001059559128161415	0.0	0.3405768287514346	1.666722224074136E-4
84-85	1.7857738115080026E-4	0.0010774168662764949	0.0	0.3409161257756211	1.666722224074136E-4
86-87	1.7857738115080026E-4	0.0011428952393651218	0.0	0.3410827979980285	1.666722224074136E-4
88-89	1.7857738115080026E-4	0.0012500416680556019	0.0	0.34110660831551526	1.666722224074136E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12660	0.0	52.0502	1
GTATCAA	22880	0.0	34.666924	1
ATCAACG	27790	0.0	29.667313	3
TCAACGC	27985	0.0	29.521622	4
TATCAAC	28240	0.0	29.102278	2
CAACGCA	28850	0.0	28.591316	5
AACGCAG	30090	0.0	27.459536	6
TGGTATC	5520	0.0	25.65364	2
GTGGTAT	5860	0.0	24.016954	1
ACGCAGA	35525	0.0	23.27084	7
CGCAGAG	36165	0.0	22.859024	8
GAGTACT	20850	0.0	20.564125	12-13
GCAGAGT	41765	0.0	19.866776	9
CAGAGTA	40830	0.0	18.721292	10-11
GTACTTT	23260	0.0	18.321445	14-15
AGAGTAC	38230	0.0	16.814476	10-11
GTACATG	31735	0.0	16.370548	1
GGACCGA	1410	0.0	16.320496	6
TACATGG	31900	0.0	16.176937	2
CTTATAC	7770	0.0	16.044693	1
>>END_MODULE