FastQCFastQC Report
Fri 27 May 2016
SRR2140167_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140167_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8931297
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114600.050.8715441
GTATCAA206300.034.499371
TCAACGC254500.030.1617054
ATCAACG256000.029.9858153
TATCAAC258850.029.421232
CAACGCA264250.028.9996245
AACGCAG274000.028.0240736
ACGCAGA326450.023.5480547
CGCAGAG337550.022.7865378
TGGTATC57250.022.3348882
GTGGTAT59700.022.289551
GCAGAGT382100.020.0390729
GAGTACT218600.019.28771612-13
CAGAGTA378050.018.19054810-11
GTACTTT240100.017.44336314-15
AGAGTAC352550.016.93739510-11
TACATGG270100.015.9514092
GTACATG266800.015.6450771
ACATGGG271300.015.4175293
AGTACTT239400.015.35839412-13
Produced by FastQC (version 0.11.5)