##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140167_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8931297
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.409789977872194	18.0	18.0	32.0	18.0	33.0
2	27.82760588971568	29.0	25.0	32.0	18.0	33.0
3	29.85896426913135	31.0	29.0	33.0	25.0	33.0
4	30.513017762145857	32.0	31.0	33.0	27.0	33.0
5	31.465155956632056	33.0	32.0	33.0	28.0	33.0
6	35.73973119469658	37.0	36.0	38.0	31.0	38.0
7	36.070078287621605	38.0	37.0	38.0	33.0	38.0
8	36.58603996709549	38.0	37.0	38.0	34.0	38.0
9	36.74781187995428	38.0	38.0	38.0	34.0	38.0
10-11	36.82254335512524	38.0	38.0	38.0	35.0	38.0
12-13	36.85499216967032	38.0	38.0	38.0	35.0	38.0
14-15	36.87735784623443	38.0	38.0	38.0	35.0	38.0
16-17	36.86888970325363	38.0	38.0	38.0	35.0	38.0
18-19	36.87735219196047	38.0	38.0	38.0	35.0	38.0
20-21	36.879092159161075	38.0	38.0	38.0	35.0	38.0
22-23	36.885747542036526	38.0	38.0	38.0	35.0	38.0
24-25	36.88263392294735	38.0	38.0	38.0	35.0	38.0
26-27	36.83645560329238	38.0	38.0	38.0	35.0	38.0
28-29	36.81770245062363	38.0	38.0	38.0	35.0	38.0
30-31	36.8076804451728	38.0	38.0	38.0	35.0	38.0
32-33	36.79019510380394	38.0	38.0	38.0	35.0	38.0
34-35	36.76194941216568	38.0	38.0	38.0	35.0	38.0
36-37	36.73723911101543	38.0	38.0	38.0	34.5	38.0
38-39	36.7241211936909	38.0	38.0	38.0	34.5	38.0
40-41	36.7135785183452	38.0	38.0	38.0	34.0	38.0
42-43	36.668775149692365	38.0	38.0	38.0	34.0	38.0
44-45	36.606339421519905	38.0	38.0	38.0	34.0	38.0
46-47	36.56211049718254	38.0	38.0	38.0	34.0	38.0
48-49	36.53077954591083	38.0	38.0	38.0	34.0	38.0
50-51	36.51825687469763	38.0	38.0	38.0	34.0	38.0
52-53	36.48471971264788	38.0	38.0	38.0	34.0	38.0
54-55	36.456775794017496	38.0	38.0	38.0	34.0	38.0
56-57	36.4296566478769	38.0	38.0	38.0	34.0	38.0
58-59	36.4119129102759	38.0	38.0	38.0	34.0	38.0
60-61	36.41626097611112	38.0	38.0	38.0	34.0	38.0
62-63	36.40127430756257	38.0	38.0	38.0	34.0	38.0
64-65	36.39301588147494	38.0	38.0	38.0	34.0	38.0
66-67	36.36769508022763	38.0	38.0	38.0	34.0	38.0
68-69	36.359472179163916	38.0	38.0	38.0	34.0	38.0
70-71	36.348577106029694	38.0	38.0	38.0	34.0	38.0
72-73	36.33322942602106	38.0	38.0	38.0	33.0	38.0
74-75	36.325469355326575	38.0	38.0	38.0	33.0	38.0
76-77	36.30388396679611	38.0	37.0	38.0	33.0	38.0
78-79	36.318327870801696	38.0	37.0	38.0	33.0	38.0
80-81	36.27539845389306	38.0	37.0	38.0	33.0	38.0
82-83	36.27668745834661	38.0	37.0	38.0	33.0	38.0
84-85	36.263060313423736	38.0	37.0	38.0	33.0	38.0
86-87	36.2241389299481	38.0	37.0	38.0	33.0	38.0
88-89	36.21914453601035	38.0	37.0	38.0	33.0	38.0
90-91	36.21917864990358	38.0	37.0	38.0	33.0	38.0
92-93	36.20182737801946	38.0	37.0	38.0	33.0	38.0
94-95	36.18316545091704	38.0	37.0	38.0	33.0	38.0
96-97	36.19088878256427	38.0	37.0	38.0	33.0	38.0
98-99	36.22229549917213	38.0	37.0	38.0	33.0	38.0
100-101	35.62758702653875	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	29.0
19	45.0
20	116.0
21	468.0
22	1912.0
23	5693.0
24	12636.0
25	23828.0
26	39356.0
27	60136.0
28	86334.0
29	116194.0
30	150039.0
31	192727.0
32	244232.0
33	323209.0
34	459834.0
35	746831.0
36	1812249.0
37	4655425.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.33905579447196	26.36023636880511	14.191813350289436	23.108894486433492
2	14.405802427127885	20.858806957153032	39.60014989984097	25.13524071587811
3	17.86941792382395	25.39670827307503	31.364642868499775	25.369230934601244
4	13.66371535959447	18.23934418483676	38.302499625754244	29.794440829814526
5	14.020028756517512	36.6840041339314	35.01158089946274	14.284386210088348
6	30.00978469308545	38.32526227713623	18.203134438368807	13.46181859140951
7	26.369316796877317	32.94018774652774	22.963831568919947	17.72666388767499
8	23.9351686546758	37.73362368310001	20.52748889662946	17.803718765594738
9	24.92242728015875	17.542043445649607	21.50267760662309	36.03285166756855
10-11	24.132393551842867	27.198353967889986	28.47934946955067	20.189903010716474
12-13	24.82030583277705	24.587287877691967	28.967720885883626	21.62468540364736
14-15	22.014311023359763	25.986208945912338	26.66164835857547	25.337831672152433
16-17	21.481947134889815	29.14840924000176	27.427617735699528	21.942025889408896
18-19	21.495388631684737	28.25731245976928	29.220817536355582	21.0264813721904
20-21	22.330485604235914	27.27139383986488	29.268028876231693	21.13009167966751
22-23	22.060856233244436	27.156714080285617	29.11666672731818	21.665762959151767
24-25	21.793753357653546	27.351639654731734	29.20652295326258	21.648084034352138
26-27	21.69936021064952	27.483010468918838	29.122872112294896	21.694757208136743
28-29	21.67739286452501	27.50843186142899	29.15117861206043	21.662996661985567
30-31	21.898559468629724	27.345662098843736	29.131638196757553	21.624140235768984
32-33	21.791658437759015	27.455916084689107	29.00775325993934	21.74467221761254
34-35	21.856668069645956	27.49027087823219	29.044199478316933	21.608861573804923
36-37	21.87381857706299	27.395070994724062	29.03316387337079	21.697946554842154
38-39	21.738221587345997	27.527732921225656	29.078187863350145	21.655857628078202
40-41	21.991708052189114	27.409066700568125	28.913321998676224	21.685903248566536
42-43	21.80641637326916	27.559780011834263	28.7479744887538	21.88582912614278
44-45	21.894256451910003	27.618706527899356	28.651534774001135	21.83550224618951
46-47	21.977596192091127	27.604488239800297	28.436609478548498	21.98130608956008
48-49	21.960606617310326	27.7079188293909	28.407268791396767	21.924205761902005
50-51	21.969460293871524	27.757185062618923	28.295407471064493	21.977947172445063
52-53	22.046416383150046	27.75283251170438	28.1967673397456	22.00398376539998
54-55	22.038010177688545	27.731513946067075	28.205989646325598	22.02448622991879
56-57	22.03035608242381	27.78552358098772	28.21043765816398	21.973682678424492
58-59	21.961650535373842	27.8302835064858	28.260180336258745	21.947885621881618
60-61	22.06971537979136	27.747655065096353	28.28706093688974	21.895568618222544
62-63	22.058381436833706	27.750291886079108	28.305230808346753	21.886095868740433
64-65	21.993417422568832	27.681360066560135	28.321346425260874	22.00387608561016
66-67	22.070960139533014	27.765110768643613	28.139281522028803	22.02464756979457
68-69	22.012216231611905	27.725902429539584	28.205460089324667	22.05642124952384
70-71	21.986687824807515	27.554027372542627	28.268185210305546	22.19109959234431
72-73	22.03763354218564	27.625076774419444	28.19669913682577	22.140590546569147
74-75	21.9320489198281	27.72654188880367	28.18179112691139	22.159618064456843
76-77	22.022234881954507	27.694576943326876	28.11497939596749	22.16820877875112
78-79	21.977612565094358	27.718514106468096	28.08681400257228	22.217059325865275
80-81	22.018343884536563	27.734020450211656	28.059476033350496	22.18815963190129
82-83	22.057461176651795	27.7084123706117	28.178975010368408	22.0551514423681
84-85	22.14091978427111	27.7172844763902	28.08449917104923	22.057296568289456
86-87	22.041828868499476	27.773046567463783	28.076382370965124	22.108742193071617
88-89	22.099119739135908	27.744880561717416	28.03718089837898	22.11881880076769
90-91	22.132997078502058	27.796569865442894	28.040717131704213	22.029715924350835
92-93	22.14164019643349	27.74619132281349	28.04320944454977	22.068959036203246
94-95	21.986065864245614	27.862292026817727	28.043921489617755	22.107720619318908
96-97	22.03409474768265	27.88498936091707	28.049829765202954	22.03108612619732
98-99	22.343117454522073	28.407712243842088	28.086791887073293	21.162378414562554
100-101	22.87642258748484	30.963190267726386	25.5004258756684	20.65996126912037
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2081.0
1	2023.5
2	2337.5
3	2804.0
4	3325.5
5	4392.5
6	5716.5
7	7880.5
8	10253.0
9	11692.0
10	11727.0
11	11266.0
12	12021.0
13	15111.5
14	21831.0
15	29528.5
16	34849.0
17	37516.5
18	37523.5
19	35198.5
20	32260.0
21	30491.0
22	31229.0
23	35342.5
24	43694.0
25	56460.5
26	71695.5
27	88642.0
28	107758.0
29	128750.5
30	149686.0
31	170308.5
32	191669.5
33	214179.5
34	234387.0
35	252341.0
36	274333.5
37	294322.0
38	308753.5
39	323011.5
40	337050.0
41	349098.0
42	356385.0
43	361896.0
44	367948.5
45	373878.0
46	377360.0
47	376731.5
48	374302.5
49	369837.0
50	362459.5
51	350323.5
52	331292.0
53	310056.0
54	288107.5
55	263875.5
56	240074.0
57	214666.0
58	187948.0
59	161301.5
60	134574.5
61	110064.5
62	87966.0
63	68474.0
64	52498.0
65	39310.0
66	29415.0
67	22396.5
68	16492.5
69	12154.0
70	9185.5
71	6520.5
72	4483.0
73	2966.0
74	1959.5
75	1279.0
76	798.5
77	482.0
78	307.0
79	211.0
80	142.0
81	88.0
82	55.5
83	41.5
84	31.0
85	23.5
86	16.5
87	10.5
88	9.5
89	9.0
90	5.5
91	1.5
92	0.0
93	0.0
94	0.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0033701712080563442
4	0.0
5	0.036209746467954204
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.1196582086565927E-5
12-13	1.231624029522252E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	4.6354381448003104E-5
46-47	0.0010516134203633022
48-49	1.1780210718519227E-5
50-51	7.715338205114403E-5
52-53	0.0015060364270767898
54-55	7.824207628951517E-4
56-57	0.003869045778088757
58-59	0.008379269577054076
60-61	0.010459169876999424
62-63	0.006225605000942221
64-65	0.007912178199515988
66-67	0.004905965628981368
68-69	0.002486867938026488
70-71	0.004132211784323697
72-73	0.0026016871358608786
74-75	0.0014879977319781938
76-77	0.001770985153614199
78-79	7.64129400463408E-4
80-81	4.1595024269019444E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	2.064599172309311E-4
94-95	2.5184276952213195E-4
96-97	1.0927184737607697E-4
98-99	6.685013649070284E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	81.0
20-21	292.0
22-23	587.0
24-25	996.0
26-27	2244.0
28-29	5495.0
30-31	15477.0
32-33	20047.0
34-35	27889.0
36-37	44790.0
38-39	53496.0
40-41	53135.0
42-43	60564.0
44-45	69885.0
46-47	70929.0
48-49	65387.0
50-51	59553.0
52-53	57761.0
54-55	61762.0
56-57	64032.0
58-59	63689.0
60-61	62561.0
62-63	61125.0
64-65	63512.0
66-67	67588.0
68-69	68664.0
70-71	67893.0
72-73	64536.0
74-75	65644.0
76-77	68840.0
78-79	71759.0
80-81	71875.0
82-83	69089.0
84-85	68132.0
86-87	70786.0
88-89	73703.0
90-91	76096.0
92-93	73579.0
94-95	79666.0
96-97	130965.0
98-99	749438.0
100-101	6007755.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.54883509005035
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.77813459711518	74.17309727056669
2	7.6774174146898995	12.82878562994814
3	1.9241703431853014	4.822865720638629
4	0.7327233525137666	2.44872730183206
5	0.3474088423472447	1.4512802039047623
6	0.18232039495177066	0.9139593966827002
7	0.1005013786750683	0.5877741179272189
8	0.06245386638791923	0.41743582268642415
9	0.04408897516803221	0.33152242640429225
>10	0.14867024663046458	1.8259740519033711
>50	0.0015646164585305352	0.08550700561290187
>100	5.008124662454585E-4	0.08162296324491075
>500	4.5159410597357654E-5	0.03144808864792359
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.1196582086565927E-5	0.0	0.0	0.0
3	0.0	1.1196582086565927E-5	0.0	0.0	0.0
4	0.0	3.358974625969778E-5	0.0	0.0	0.0
5	0.0	4.4786328346263706E-5	0.0	0.0	0.0
6	0.0	4.4786328346263706E-5	0.0	0.0	0.0
7	0.0	5.598291043282963E-5	0.0	0.0	0.0
8	0.0	5.598291043282963E-5	0.0	0.0	1.1196582086565927E-5
9	0.0	7.837607460596149E-5	0.0	0.0	2.2393164173131853E-5
10-11	0.0	7.837607460596149E-5	0.0	0.0	2.2393164173131853E-5
12-13	0.0	1.0076923877909334E-4	0.0	0.0	3.358974625969778E-5
14-15	0.0	1.5115385816864E-4	0.0	0.0	6.717949251939556E-5
16-17	0.0	1.5675214921192297E-4	0.0	0.0	8.957265669252741E-5
18-19	0.0	1.959401865149037E-4	0.0	0.0	1.0076923877909334E-4
20-21	0.0	2.3512822381788445E-4	0.0	0.0	1.1756411190894222E-4
22-23	0.0	2.631196790342993E-4	0.0	0.0	1.3435898503879113E-4
24-25	0.0	3.07906007380563E-4	0.0	5.598291043282963E-6	1.5675214921192297E-4
26-27	0.0	3.358974625969778E-4	0.0	3.9188037302980744E-5	1.5675214921192297E-4
28-29	0.0	3.4149575364026075E-4	0.0	2.631196790342993E-4	1.6235044025520595E-4
30-31	0.0	3.806837909432415E-4	0.0	8.789316937954253E-4	1.679487312984889E-4
32-33	0.0	3.806837909432415E-4	0.0	0.0024856412232176356	1.7914531338505482E-4
34-35	0.0	4.142735372029393E-4	0.0	0.004338675558544296	2.0153847755818667E-4
36-37	0.0	4.5346157450592E-4	0.0	0.007076239878709666	2.0153847755818667E-4
38-39	0.0	4.702564476357689E-4	0.0	0.011398120564124113	2.0153847755818667E-4
40-41	0.0	5.318376491118815E-4	0.0	0.016347009846386252	2.0153847755818667E-4
42-43	0.0	5.430342311984475E-4	0.0	0.020825642681012623	2.0713676860146965E-4
44-45	0.0	6.550000520641067E-4	0.0	0.02623359182882397	2.1833335068803555E-4
46-47	0.0	7.725641639730489E-4	0.0	0.03178149825271738	2.2393164173131852E-4
48-49	0.0	7.725641639730489E-4	0.0	0.037698891885467475	2.2393164173131852E-4
50-51	0.0	8.509402385790104E-4	0.0	0.043537909443611605	2.2393164173131852E-4
52-53	0.0	8.509402385790104E-4	0.0	0.04893466200933638	2.407265148611674E-4
54-55	0.0	8.845299848387082E-4	0.0	0.05471769665704768	2.575213879910163E-4
56-57	0.0	9.069231490118401E-4	0.0	0.060590303961451514	2.575213879910163E-4
58-59	0.0	9.349146042282549E-4	0.0	0.06645731297481206	2.631196790342993E-4
60-61	0.0	9.629060594446697E-4	0.0	0.07189885186888309	2.6871797007758225E-4
62-63	0.0	0.0010188889698774994	0.0	0.07759231385990187	2.7991455216414815E-4
64-65	0.0	0.001063675298223763	0.0	0.08317940832109827	2.7991455216414815E-4
66-67	0.0	0.001074871880310329	0.0	0.08890646005837674	2.7991455216414815E-4
68-69	0.0	0.0010916667534401777	0.0	0.09429201604201495	2.7991455216414815E-4
70-71	0.0	0.0011252564996998755	0.0	0.09954321304061436	2.7991455216414815E-4
72-73	0.0	0.0011588462459595733	0.0	0.10386509372602881	3.1350429842384595E-4
74-75	0.0	0.0011700428280461393	0.0	0.10729124784451799	3.1350429842384595E-4
76-77	0.0	0.001214829156392403	0.0	0.11033671817206392	3.1350429842384595E-4
78-79	0.0	0.0012428206116088178	0.0	0.11299590641762333	3.1350429842384595E-4
80-81	0.0	0.0012428206116088178	0.0	0.11422753044714559	3.191025894671289E-4
82-83	0.0	0.001248418902652101	0.0	0.11460821423808883	3.5829062677010965E-4
84-85	0.0	0.0012540171936953838	0.0	0.1149441117006858	3.5829062677010965E-4
86-87	0.0	0.0012540171936953838	0.0	0.11512885530511413	3.5829062677010965E-4
88-89	0.0	0.0012708120668252327	0.0	0.11517923992450368	3.5829062677010965E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11460	0.0	50.871544	1
GTATCAA	20630	0.0	34.49937	1
TCAACGC	25450	0.0	30.161705	4
ATCAACG	25600	0.0	29.985815	3
TATCAAC	25885	0.0	29.42123	2
CAACGCA	26425	0.0	28.999624	5
AACGCAG	27400	0.0	28.024073	6
ACGCAGA	32645	0.0	23.548054	7
CGCAGAG	33755	0.0	22.786537	8
TGGTATC	5725	0.0	22.334888	2
GTGGTAT	5970	0.0	22.28955	1
GCAGAGT	38210	0.0	20.039072	9
GAGTACT	21860	0.0	19.287716	12-13
CAGAGTA	37805	0.0	18.190548	10-11
GTACTTT	24010	0.0	17.443363	14-15
AGAGTAC	35255	0.0	16.937395	10-11
TACATGG	27010	0.0	15.951409	2
GTACATG	26680	0.0	15.645077	1
ACATGGG	27130	0.0	15.417529	3
AGTACTT	23940	0.0	15.358394	12-13
>>END_MODULE