FastQCFastQC Report
Fri 27 May 2016
SRR2140166_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140166_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7805400
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107350.052.016791
GTATCAA190650.034.4526061
TCAACGC230500.030.026134
ATCAACG233250.029.6355383
CAACGCA239850.028.8919535
TATCAAC237300.028.8177092
AACGCAG248000.027.947866
ACGCAGA289600.023.9185267
CGCAGAG295650.023.4437968
GTGGTAT49500.023.4063641
TGGTATC48300.022.9057312
GCAGAGT339300.020.4534789
GAGTACT196150.020.43140412-13
CAGAGTA336950.018.88409210-11
GTACTTT215200.018.45092414-15
AGAGTAC314550.017.19766410-11
ACTTTTT252200.015.82265516-17
AGTACTT216700.015.53042412-13
TACATGG233850.013.9322662
GTACATG232400.013.9254821
Produced by FastQC (version 0.11.5)