##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140166_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7805400
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.620794578112587	18.0	18.0	32.0	18.0	33.0
2	27.975580239321495	30.0	25.0	32.0	18.0	33.0
3	29.959868936889844	31.0	29.0	33.0	25.0	33.0
4	30.583371768262996	32.0	31.0	33.0	27.0	33.0
5	31.46636187767443	33.0	32.0	33.0	28.0	33.0
6	35.740471468470545	37.0	36.0	38.0	31.0	38.0
7	36.06780421246829	38.0	37.0	38.0	33.0	38.0
8	36.577274194788224	38.0	37.0	38.0	34.0	38.0
9	36.73935480564737	38.0	38.0	38.0	34.0	38.0
10-11	36.81724876367643	38.0	38.0	38.0	35.0	38.0
12-13	36.84561495887462	38.0	38.0	38.0	35.0	38.0
14-15	36.867806134214774	38.0	38.0	38.0	35.0	38.0
16-17	36.85817337996771	38.0	38.0	38.0	35.0	38.0
18-19	36.866550593179085	38.0	38.0	38.0	35.0	38.0
20-21	36.86866720149882	38.0	38.0	38.0	35.0	38.0
22-23	36.876819480102995	38.0	38.0	38.0	35.0	38.0
24-25	36.87237460759374	38.0	38.0	38.0	35.0	38.0
26-27	36.823880244262654	38.0	38.0	38.0	35.0	38.0
28-29	36.80406133949633	38.0	38.0	38.0	35.0	38.0
30-31	36.794141605155176	38.0	38.0	38.0	35.0	38.0
32-33	36.77671621843041	38.0	38.0	38.0	35.0	38.0
34-35	36.74865274979287	38.0	38.0	38.0	35.0	38.0
36-37	36.72060195857312	38.0	38.0	38.0	34.5	38.0
38-39	36.70648872468838	38.0	38.0	38.0	34.0	38.0
40-41	36.69263974751682	38.0	38.0	38.0	34.0	38.0
42-43	36.6478131407666	38.0	38.0	38.0	34.0	38.0
44-45	36.58360822789588	38.0	38.0	38.0	34.0	38.0
46-47	36.535882714625316	38.0	38.0	38.0	34.0	38.0
48-49	36.50116530767707	38.0	38.0	38.0	34.0	38.0
50-51	36.48754843882011	38.0	38.0	38.0	34.0	38.0
52-53	36.45139921947427	38.0	38.0	38.0	34.0	38.0
54-55	36.422871216233055	38.0	38.0	38.0	34.0	38.0
56-57	36.394545407209236	38.0	38.0	38.0	34.0	38.0
58-59	36.37623172254097	38.0	38.0	38.0	34.0	38.0
60-61	36.379631361790985	38.0	38.0	38.0	34.0	38.0
62-63	36.36463508084171	38.0	38.0	38.0	34.0	38.0
64-65	36.35389897659549	38.0	38.0	38.0	34.0	38.0
66-67	36.32733913036909	38.0	38.0	38.0	33.0	38.0
68-69	36.32224137335456	38.0	38.0	38.0	33.0	38.0
70-71	36.31143362565909	38.0	37.5	38.0	33.0	38.0
72-73	36.29233493713103	38.0	37.0	38.0	33.0	38.0
74-75	36.2834856749877	38.0	37.0	38.0	33.0	38.0
76-77	36.26381593300894	38.0	37.0	38.0	33.0	38.0
78-79	36.27292651858616	38.0	37.0	38.0	33.0	38.0
80-81	36.23196159734799	38.0	37.0	38.0	33.0	38.0
82-83	36.234117722462784	38.0	37.0	38.0	33.0	38.0
84-85	36.21990144320556	38.0	37.0	38.0	33.0	38.0
86-87	36.18498489371332	38.0	37.0	38.0	33.0	38.0
88-89	36.176673112738	38.0	37.0	38.0	33.0	38.0
90-91	36.178096551673875	38.0	37.0	38.0	33.0	38.0
92-93	36.158008829601876	38.0	37.0	38.0	33.0	38.0
94-95	36.13746528067822	38.0	37.0	38.0	33.0	38.0
96-97	36.14582388047175	38.0	37.0	38.0	33.0	38.0
98-99	36.18128797858396	38.0	37.0	38.0	33.0	38.0
100-101	35.580526370892315	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	5.0
18	29.0
19	40.0
20	93.0
21	396.0
22	1689.0
23	4940.0
24	10987.0
25	21416.0
26	35415.0
27	54268.0
28	77894.0
29	104361.0
30	135068.0
31	170646.0
32	218134.0
33	287066.0
34	404600.0
35	656961.0
36	1567870.0
37	4053522.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.7765136956466	25.74946831680631	14.370973941117688	23.103044046429392
2	14.500896815025497	20.791618623004588	39.53912675839803	25.168357803571883
3	17.967753061314436	25.15279666845352	31.37719175969079	25.502258510541253
4	13.664565557178362	18.25310682348118	38.236579803725625	29.845747815614832
5	14.09781126283315	36.587014199434456	34.981357546883615	14.333816990848785
6	30.13900632895175	38.27847387705947	18.149973095549235	13.432546698439543
7	26.575793681297565	32.88593794040023	22.73643887565019	17.801829502652012
8	24.087272913623902	37.67581417992672	20.381095139262563	17.855817767186817
9	24.998744458964307	17.470007943218796	21.48971225049325	36.04153534732365
10-11	24.242711727951914	27.18382960410931	28.35222591745624	20.221232750482535
12-13	24.894230127415813	24.507228275233945	28.871693646530623	21.726847950819618
14-15	22.056211084633716	25.967996515233043	26.626636687421527	25.349155712711713
16-17	21.528480282881084	29.105664027468166	27.389634099469596	21.976221590181154
18-19	21.487649575934608	28.138468239936454	29.279940810208316	21.093941373920618
20-21	22.399260602652653	27.177573680775264	29.290599671978267	21.132566044593815
22-23	22.049460647346184	27.091727619734858	29.168932333894347	21.689879399024615
24-25	21.775728166162818	27.26514636744347	29.26052788611197	21.698597580281735
26-27	21.782810476500423	27.37727190214879	29.157751294318874	21.68216632703191
28-29	21.719651857417862	27.434428739280786	29.21458064832893	21.631338754972422
30-31	21.965845554810546	27.256299856839806	29.160889835934157	21.61696475241549
32-33	21.784972498756897	27.410264157674906	29.073467009882247	21.731296333685947
34-35	21.85638834883427	27.45680557698743	29.076469244107578	21.61033683007072
36-37	21.930374499642078	27.291100688562807	29.07364981711	21.70487499468512
38-39	21.741228492043426	27.47923381467633	29.098021096860894	21.68151659641935
40-41	22.011014810940672	27.377538176823208	28.950087072468435	21.661359939767692
42-43	21.851209753250288	27.49945529393535	28.769833984364094	21.87950096845027
44-45	21.961760397342672	27.550244867050917	28.631356870992597	21.856637864613816
46-47	22.038001772407302	27.50352865030751	28.45263643086895	22.00583314641624
48-49	21.968462603119885	27.6612881828633	28.42108480289211	21.949164411124706
50-51	21.9297126407544	27.74326982089297	28.298230199131684	22.028787339220944
52-53	22.111238783941822	27.742039599005174	28.161262701318773	21.98545891573423
54-55	22.11945539797595	27.636066713809633	28.230076321845132	22.014401566369283
56-57	22.100394590360693	27.733023909174214	28.220307589668554	21.94627391079654
58-59	22.023877414085486	27.7841613403599	28.248833850385672	21.943127395168936
60-61	22.076927695846855	27.713888683947722	28.28202762690955	21.927155993295866
62-63	22.132037102678346	27.762629572550573	28.219465632290337	21.885867692480744
64-65	22.03311888775919	27.715427891950135	28.249017474891815	22.00243574539886
66-67	22.10832449878723	27.764138604180395	28.128893986449448	21.998642910582934
68-69	22.079386960652972	27.632535987156814	28.212972020375048	22.075105031815166
70-71	22.020535157230764	27.446614474865388	28.285466414465194	22.247383953438653
72-73	22.14389336403121	27.53574648408478	28.2120517556817	22.10830839620231
74-75	21.916524812807666	27.657802613426107	28.218062458320713	22.207610115445508
76-77	22.083207117244424	27.6610952522357	28.12352364481226	22.132173985707613
78-79	21.996056804120506	27.74426629165182	28.051691951389685	22.20798495283799
80-81	22.08014064442886	27.668495878878787	28.059929465074696	22.191434011617662
82-83	22.141199842006614	27.663340075856453	28.133011601719282	22.06244848041765
84-85	22.232017240263076	27.653439834237048	28.04501366416619	22.069529261333685
86-87	22.044987451282896	27.742724686344506	28.036740371031204	22.175547491341394
88-89	22.14759728816668	27.67047177413605	28.05026299571129	22.131667941985985
90-91	22.161317209702027	27.775531679991406	28.03894974925353	22.024201361053038
92-93	22.226272199586028	27.711717323227937	28.022087258111107	22.039923219074932
94-95	22.01259756894065	27.83750505526453	28.063595897890924	22.086301477903895
96-97	22.024860565575977	27.86174082483104	28.067849126914545	22.045549482678435
98-99	22.356207001160517	28.370629103450344	28.059681590763592	21.21348230462555
100-101	22.92013654595206	30.94837763219762	25.45529707199197	20.676188749858344
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2142.0
1	2045.0
2	2190.5
3	2469.0
4	2893.5
5	3834.0
6	5007.0
7	7108.5
8	9365.5
9	10681.0
10	10602.0
11	10125.0
12	10990.0
13	13519.5
14	19229.0
15	26258.5
16	31249.0
17	33700.0
18	33661.0
19	31892.5
20	29728.0
21	27885.5
22	27988.0
23	31637.5
24	39225.0
25	50591.0
26	64718.0
27	80391.5
28	97462.0
29	115082.5
30	131716.5
31	148543.5
32	166307.5
33	185920.5
34	203958.0
35	219641.5
36	239065.0
37	255599.0
38	265855.0
39	275815.5
40	286310.0
41	296157.0
42	302101.0
43	306655.0
44	312198.0
45	316631.5
46	317428.5
47	316741.0
48	315341.0
49	311277.5
50	305414.0
51	296549.0
52	282631.5
53	266035.0
54	250349.5
55	233545.0
56	214660.0
57	193969.5
58	172721.0
59	149956.5
60	125972.5
61	102981.5
62	81100.0
63	62443.5
64	47673.5
65	36613.0
66	28414.5
67	21157.5
68	15180.0
69	11141.5
70	8039.5
71	5452.5
72	3717.5
73	2467.5
74	1585.5
75	1011.5
76	635.5
77	373.5
78	233.5
79	149.5
80	93.0
81	57.0
82	41.0
83	25.5
84	17.5
85	13.0
86	10.0
87	8.5
88	6.5
89	5.0
90	4.5
91	3.5
92	3.0
93	3.5
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0032797806646680506
4	0.0
5	0.039037076895482616
6	0.0
7	0.0
8	0.0
9	0.0
10-11	6.405821610679786E-6
12-13	1.4092807543495528E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	1.9840675408335983E-5
46-47	8.731979294410747E-4
48-49	1.343417292212136E-5
50-51	6.0885745530732585E-5
52-53	0.0013209707718874813
54-55	6.648498673041915E-4
56-57	0.0037346550376458065
58-59	0.008490862239758723
60-61	0.010788900519261376
62-63	0.006371884339079649
64-65	0.008251144073361697
66-67	0.00514934054422442
68-69	0.0025127719940297114
70-71	0.004550895430544198
72-73	0.002701653470655638
74-75	0.0013541421320901273
76-77	0.0019845998039573517
78-79	7.602408202050031E-4
80-81	4.7100042769877544E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	2.1705830684552057E-4
94-95	3.3302294511846844E-4
96-97	1.3973424683364253E-4
98-99	5.506464250170361E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	71.0
20-21	285.0
22-23	583.0
24-25	875.0
26-27	1989.0
28-29	4590.0
30-31	12464.0
32-33	15979.0
34-35	22435.0
36-37	36666.0
38-39	43211.0
40-41	42961.0
42-43	48881.0
44-45	58301.0
46-47	58479.0
48-49	54284.0
50-51	48532.0
52-53	47360.0
54-55	51239.0
56-57	53113.0
58-59	52824.0
60-61	51919.0
62-63	50115.0
64-65	52615.0
66-67	56737.0
68-69	57608.0
70-71	57241.0
72-73	53509.0
74-75	54633.0
76-77	58175.0
78-79	60680.0
80-81	60645.0
82-83	58042.0
84-85	57224.0
86-87	59024.0
88-89	62215.0
90-91	64734.0
92-93	63226.0
94-95	67965.0
96-97	112973.0
98-99	662338.0
100-101	5328660.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.19687889517526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.1406397322733	75.05363648176663
2	7.501871594971413	12.632683483379267
3	1.8521220382403087	4.678286848584133
4	0.6995074504418042	2.355853763644856
5	0.30769491517555314	1.2953475754848638
6	0.17205827194256298	0.8692061691396665
7	0.09851803388115155	0.5806437677377463
8	0.058282732721549414	0.3925779350908932
9	0.04352866705376937	0.3298480117555162
>10	0.1236484316166252	1.5744787834000566
>50	0.0015327244960586935	0.08522969645045038
>100	5.205171806939243E-4	0.08711366081157579
>500	2.4963344068986184E-5	0.014145353974268492
>1k	4.9926661135813755E-5	0.050948468780151454
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.281164322135957E-5	1.281164322135957E-5
2	0.0	1.281164322135957E-5	0.0	1.281164322135957E-5	1.281164322135957E-5
3	0.0	1.281164322135957E-5	0.0	1.281164322135957E-5	1.281164322135957E-5
4	0.0	1.4092807543495528E-4	0.0	1.281164322135957E-5	1.281164322135957E-5
5	0.0	1.6655136187767442E-4	0.0	1.281164322135957E-5	1.281164322135957E-5
6	0.0	1.6655136187767442E-4	0.0	1.281164322135957E-5	2.562328644271914E-5
7	1.281164322135957E-5	1.79363005099034E-4	0.0	1.281164322135957E-5	2.562328644271914E-5
8	1.281164322135957E-5	2.0498629154175313E-4	0.0	1.281164322135957E-5	5.124657288543828E-5
9	3.8434929664078716E-5	2.9466779409127015E-4	0.0	3.8434929664078716E-5	7.686985932815743E-5
10-11	5.7652394496118074E-5	2.9466779409127015E-4	0.0	3.8434929664078716E-5	7.686985932815743E-5
12-13	7.686985932815743E-5	3.0747943731262973E-4	0.0	4.48407512747585E-5	7.686985932815743E-5
14-15	7.686985932815743E-5	3.971609398621467E-4	0.0	7.686985932815743E-5	1.4092807543495528E-4
16-17	7.686985932815743E-5	4.0997258308350627E-4	0.0	1.1530478899223615E-4	1.79363005099034E-4
18-19	7.686985932815743E-5	4.548133343582648E-4	0.0	1.345222538242755E-4	1.9217464832039355E-4
20-21	7.686985932815743E-5	4.996540856330233E-4	0.0	1.729571834883542E-4	2.1139211315243292E-4
22-23	7.686985932815743E-5	5.509006585184615E-4	0.0	2.1139211315243292E-4	2.4342122120583185E-4
24-25	7.686985932815743E-5	5.829297665718605E-4	0.0	2.1779793476311271E-4	2.8185615086991057E-4
26-27	8.327568093883722E-5	6.021472314038999E-4	0.0	2.626386860378712E-4	2.8185615086991057E-4
28-29	8.9681502549517E-5	6.085530530145797E-4	0.0	4.3559586952622543E-4	2.8185615086991057E-4
30-31	8.9681502549517E-5	7.238578420068158E-4	0.0	0.001050554744151485	2.8185615086991057E-4
32-33	8.9681502549517E-5	7.49481128449535E-4	0.0	0.0023189074230660824	3.3310272375534883E-4
34-35	8.9681502549517E-5	8.9681502549517E-4	0.0	0.004195813154995259	3.715376534194276E-4
36-37	8.9681502549517E-5	0.001044148922540805	0.0	0.0067645476208778536	3.715376534194276E-4
38-39	8.9681502549517E-5	0.0010633663873728444	0.0	0.010742562841110002	3.715376534194276E-4
40-41	8.9681502549517E-5	0.0011466420683116816	0.0	0.01527147871986061	3.8434929664078716E-4
42-43	8.9681502549517E-5	0.0011914828195864402	0.0	0.019204653188817998	3.9075511825146695E-4
44-45	8.9681502549517E-5	0.0013260050734107158	0.0	0.023836062213339483	3.9716093986214674E-4
46-47	8.9681502549517E-5	0.001434904040792272	0.0	0.02933866297691342	4.2919004791554564E-4
48-49	8.9681502549517E-5	0.001434904040792272	0.0	0.03495016270786891	4.868424424116637E-4
50-51	8.9681502549517E-5	0.0015758321162272274	0.0	0.04038229943372537	4.996540856330233E-4
52-53	8.9681502549517E-5	0.001639890332334025	0.0	0.045609449868040074	5.573064801291413E-4
54-55	8.9681502549517E-5	0.0016462961539447049	0.0	0.05090706434007226	6.27770517846619E-4
56-57	1.1530478899223615E-4	0.0016655136187767442	0.0	0.05646731749814231	6.405821610679786E-4
58-59	1.4092807543495528E-4	0.0016847310836087836	0.0	0.06207241140748712	6.469879826786584E-4
60-61	1.4092807543495528E-4	0.0017423834781049017	0.0	0.06785046250032029	6.533938042893382E-4
62-63	1.5373971865631486E-4	0.0017872242293796602	0.0	0.0724114074871243	6.662054475106977E-4
64-65	1.5373971865631486E-4	0.0019986163425320933	0.0	0.07724139698157685	6.662054475106977E-4
66-67	1.5373971865631486E-4	0.002037051272196172	0.0	0.08227637276757117	6.662054475106977E-4
68-69	1.5373971865631486E-4	0.00208189202347093	0.0	0.08727931944551208	6.662054475106977E-4
70-71	1.5373971865631486E-4	0.002113921131524329	0.0	0.09213493222640737	7.17452020396136E-4
72-73	1.5373971865631486E-4	0.002145950239577728	0.0	0.0957990621877162	7.943218797242935E-4
74-75	1.5373971865631486E-4	0.002235631742127245	0.0	0.0989955671714454	7.943218797242935E-4
76-77	1.5373971865631486E-4	0.002331719066287442	0.0	0.10189099853947267	7.943218797242935E-4
78-79	1.6014554026699465E-4	0.0023701539959515208	0.0	0.1046711251185077	7.943218797242935E-4
80-81	1.6655136187767442E-4	0.0023765598175622005	0.0	0.10580495554359802	8.07133522945653E-4
82-83	1.6655136187767442E-4	0.00238937146078356	0.0	0.10623414559151356	8.583800958310913E-4
84-85	1.6655136187767442E-4	0.002421400568836959	0.0	0.1065736541368796	8.583800958310913E-4
86-87	1.6655136187767442E-4	0.002479052963333077	0.0	0.10672098803392523	8.583800958310913E-4
88-89	1.6655136187767442E-4	0.0025687344658825943	0.0	0.10677223460681066	8.775975606631306E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10735	0.0	52.01679	1
GTATCAA	19065	0.0	34.452606	1
TCAACGC	23050	0.0	30.02613	4
ATCAACG	23325	0.0	29.635538	3
CAACGCA	23985	0.0	28.891953	5
TATCAAC	23730	0.0	28.817709	2
AACGCAG	24800	0.0	27.94786	6
ACGCAGA	28960	0.0	23.918526	7
CGCAGAG	29565	0.0	23.443796	8
GTGGTAT	4950	0.0	23.406364	1
TGGTATC	4830	0.0	22.905731	2
GCAGAGT	33930	0.0	20.453478	9
GAGTACT	19615	0.0	20.431404	12-13
CAGAGTA	33695	0.0	18.884092	10-11
GTACTTT	21520	0.0	18.450924	14-15
AGAGTAC	31455	0.0	17.197664	10-11
ACTTTTT	25220	0.0	15.822655	16-17
AGTACTT	21670	0.0	15.530424	12-13
TACATGG	23385	0.0	13.932266	2
GTACATG	23240	0.0	13.925482	1
>>END_MODULE