FastQCFastQC Report
Fri 27 May 2016
SRR2140165_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140165_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9848525
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA135000.049.7390861
GTATCAA244200.035.8726771
TCAACGC298500.030.8474544
ATCAACG301000.030.5491163
TATCAAC303800.029.9810682
CAACGCA307050.029.9034545
AACGCAG317450.028.951976
ACGCAGA370000.024.8635187
CGCAGAG377400.024.3068478
GTGGTAT68450.021.2343351
GCAGAGT435350.021.0313669
TGGTATC66200.020.9042592
GAGTACT257700.020.12787212-13
CAGAGTA429450.018.88948610-11
GTACTTT286700.017.99344314-15
AGAGTAC399250.017.67639510-11
AGTACTT285750.015.61762712-13
ACTTTTT333600.015.43166216-17
TACATGG276750.014.1678122
TATACTG57300.014.0496385
Produced by FastQC (version 0.11.5)