##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140165_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9848525
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.557692649406892	18.0	18.0	32.0	18.0	33.0
2	27.979097479064123	30.0	25.0	32.0	18.0	33.0
3	29.96021861141643	31.0	29.0	33.0	25.0	33.0
4	30.585292010732573	32.0	31.0	33.0	27.0	33.0
5	31.489488629007898	33.0	32.0	33.0	28.0	33.0
6	35.75678195465819	37.0	36.0	38.0	31.0	38.0
7	36.082141335885325	38.0	37.0	38.0	33.0	38.0
8	36.58567694147093	38.0	37.0	38.0	34.0	38.0
9	36.74353255944418	38.0	38.0	38.0	34.0	38.0
10-11	36.819430270015054	38.0	38.0	38.0	35.0	38.0
12-13	36.849603163925565	38.0	38.0	38.0	35.0	38.0
14-15	36.872786686331196	38.0	38.0	38.0	35.0	38.0
16-17	36.86420250748209	38.0	38.0	38.0	35.0	38.0
18-19	36.87052705862045	38.0	38.0	38.0	35.0	38.0
20-21	36.87296712152357	38.0	38.0	38.0	35.0	38.0
22-23	36.88066403672504	38.0	38.0	38.0	35.0	38.0
24-25	36.876202265210665	38.0	38.0	38.0	35.0	38.0
26-27	36.8287411208979	38.0	38.0	38.0	35.0	38.0
28-29	36.80802880254723	38.0	38.0	38.0	35.0	38.0
30-31	36.79744837221063	38.0	38.0	38.0	35.0	38.0
32-33	36.777370780575566	38.0	38.0	38.0	35.0	38.0
34-35	36.747963875617515	38.0	38.0	38.0	35.0	38.0
36-37	36.718985162340054	38.0	38.0	38.0	34.5	38.0
38-39	36.70426541597831	38.0	38.0	38.0	34.0	38.0
40-41	36.69284177827956	38.0	38.0	38.0	34.0	38.0
42-43	36.645710679849174	38.0	38.0	38.0	34.0	38.0
44-45	36.58030362492997	38.0	38.0	38.0	34.0	38.0
46-47	36.5314983179553	38.0	38.0	38.0	34.0	38.0
48-49	36.498243164167974	38.0	38.0	38.0	34.0	38.0
50-51	36.48368892521862	38.0	38.0	38.0	34.0	38.0
52-53	36.451290630529584	38.0	38.0	38.0	34.0	38.0
54-55	36.42235200912076	38.0	38.0	38.0	34.0	38.0
56-57	36.39419877672387	38.0	38.0	38.0	34.0	38.0
58-59	36.37578295000485	38.0	38.0	38.0	34.0	38.0
60-61	36.379522582567006	38.0	38.0	38.0	34.0	38.0
62-63	36.36334059655032	38.0	38.0	38.0	34.0	38.0
64-65	36.35614527980539	38.0	38.0	38.0	34.0	38.0
66-67	36.32994674076761	38.0	38.0	38.0	33.0	38.0
68-69	36.326152232351205	38.0	38.0	38.0	33.0	38.0
70-71	36.314123997802604	38.0	38.0	38.0	33.0	38.0
72-73	36.295233167460054	38.0	37.0	38.0	33.0	38.0
74-75	36.28634091590834	38.0	37.0	38.0	33.0	38.0
76-77	36.26524759837053	38.0	37.0	38.0	33.0	38.0
78-79	36.278688643000244	38.0	37.0	38.0	33.0	38.0
80-81	36.23825513388154	38.0	37.0	38.0	33.0	38.0
82-83	36.24025895578774	38.0	37.0	38.0	33.0	38.0
84-85	36.226178638099384	38.0	37.0	38.0	33.0	38.0
86-87	36.1881887822467	38.0	37.0	38.0	33.0	38.0
88-89	36.17912814602546	38.0	37.0	38.0	33.0	38.0
90-91	36.18256193421245	38.0	37.0	38.0	33.0	38.0
92-93	36.16626077550755	38.0	37.0	38.0	33.0	38.0
94-95	36.14418034806425	38.0	37.0	38.0	33.0	38.0
96-97	36.15395793933981	38.0	37.0	38.0	33.0	38.0
98-99	36.187316969061754	38.0	37.0	38.0	33.0	38.0
100-101	35.58686019643685	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	7.0
18	36.0
19	54.0
20	100.0
21	532.0
22	2097.0
23	6357.0
24	14210.0
25	26877.0
26	45115.0
27	68499.0
28	97655.0
29	131746.0
30	170183.0
31	216316.0
32	275341.0
33	362797.0
34	511052.0
35	825703.0
36	1971823.0
37	5122025.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.76552580208711	25.810738156221362	14.324632368806498	23.099103672885025
2	14.509198077884761	20.86520570339213	39.53361543987551	25.091980778847596
3	17.932148495584784	25.217196786584207	31.38297591481581	25.467678803015207
4	13.655801249425675	18.27195442972425	38.197983962065386	29.874260358784692
5	14.04629589468615	36.63328772810276	34.96278326734644	14.357633109864656
6	30.06698972688804	38.2904851234068	18.174031136642288	13.46849401306287
7	26.421550435217455	33.02175706514427	22.765571494208523	17.791121005429748
8	23.99883231245288	37.65605509454461	20.45044308665511	17.894669506347398
9	24.960052393632548	17.494040985832903	21.468991549495993	36.076915071038556
10-11	24.170328679217754	27.187095608798618	28.413208335239286	20.229367376744342
12-13	24.84226560788131	24.57082882927157	28.883355851103687	21.70354971174343
14-15	22.020926991605343	25.99160280346549	26.641893075359	25.345577129570163
16-17	21.494051139637662	29.13890658753468	27.444063958816166	21.92297831401149
18-19	21.483577490030235	28.16556286347448	29.321807072632705	21.029052573862582
20-21	22.33193387596957	27.178892363698615	29.366164311329186	21.123009449002627
22-23	21.9884822721682	27.122809645240274	29.22940891995598	21.659299162635545
24-25	21.74511964515687	27.25739689392428	29.334819936782118	21.662663524136732
26-27	21.711139562397857	27.395999168569237	29.21251694334728	21.68034432568563
28-29	21.691495789122193	27.416916959454575	29.276100068617044	21.615487182806184
30-31	21.907393049406938	27.290004328415986	29.17913189964278	21.6234707225343
32-33	21.72798693015122	27.43231419874087	29.11200225980114	21.72769661130677
34-35	21.840700965819963	27.455379477111958	29.109211902508907	21.59470765455917
36-37	21.86006761076957	27.313879058538937	29.1347052942304	21.691348036461093
38-39	21.686865598066742	27.49871960473978	29.18325482422092	21.63115997297255
40-41	21.979489097255883	27.35916211150461	29.005027088635636	21.656321702603872
42-43	21.80915360984863	27.498382763645985	28.834027277907353	21.858436348598033
44-45	21.903801818392097	27.5294513799487	28.703226650728837	21.863520150930373
46-47	21.99558728021786	27.534834284036958	28.491765961320926	21.977812474424262
48-49	21.927719600352606	27.699069024428336	28.44117023135391	21.932041143865142
50-51	21.927930658336443	27.780027850079804	28.3114994011229	21.98054209046085
52-53	22.078098364829874	27.745751855008816	28.19379955799904	21.982350222162278
54-55	22.069605189789062	27.63173169769751	28.301698649512435	21.996964463000992
56-57	22.030411596058077	27.74512629760819	28.25514988251385	21.969312223819877
58-59	22.002611010460164	27.803545426215102	28.254243996299266	21.939599567025468
60-61	22.048899614580534	27.74654066587585	28.320769935434814	21.8837897841088
62-63	22.10967715254704	27.766290842331642	28.270863618925983	21.85316838619534
64-65	21.9993242741663	27.71445756913135	28.274265656220386	22.011952500481954
66-67	22.045908341487454	27.81848042822994	28.130275310995493	22.00533591928711
68-69	22.014692854355506	27.707327713162215	28.22198608829229	22.055993344189986
70-71	22.017580025397034	27.495793923517837	28.292458805043186	22.19416724604195
72-73	22.05093093056646	27.56466145767385	28.213163674819835	22.17124393693986
74-75	21.910248521378392	27.67012531369603	28.209353053778646	22.21027311114693
76-77	22.080501723514633	27.66773051477959	28.133156894862495	22.118610866843284
78-79	21.979684760972308	27.731342438232215	28.062916806691145	22.226055994104332
80-81	22.08713801010884	27.691122509166792	28.03956551572871	22.182173964995656
82-83	22.06752398627996	27.69321111709014	28.153840428244937	22.085424468384968
84-85	22.183068326202964	27.708067396475805	28.047085323831972	22.06177895348926
86-87	22.079334794618	27.70992068036798	28.075693410603737	22.135051114410288
88-89	22.081909782993296	27.689863062452325	28.075764450375868	22.152462704178514
90-91	22.113394856580467	27.790687833706563	28.063019330217546	22.03289797949542
92-93	22.205126927120634	27.716019102283884	28.041499540688513	22.037354429906966
94-95	22.020466341154766	27.83882375938597	28.055112256799276	22.085597642659984
96-97	22.03043740088501	27.879100967189903	28.04517726199473	22.045284369930357
98-99	22.323553998296752	28.39476339432099	28.11011588616452	21.17156672121774
100-101	22.899693668926872	30.964554280606976	25.483836810999843	20.651915239466312
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2969.0
1	2791.5
2	2906.0
3	3275.0
4	3783.0
5	4935.5
6	6537.5
7	9297.5
8	12183.5
9	13730.0
10	13603.0
11	13078.0
12	14278.5
13	17808.0
14	25303.0
15	34394.0
16	40839.0
17	43890.0
18	43715.0
19	41470.0
20	38529.5
21	36231.5
22	36538.5
23	41102.0
24	50829.5
25	65437.5
26	83167.5
27	102524.0
28	123997.0
29	146183.5
30	166515.5
31	188752.0
32	212362.0
33	236403.0
34	259593.0
35	278735.0
36	301266.0
37	321972.0
38	336479.0
39	349516.0
40	363014.0
41	376057.5
42	383299.5
43	388602.0
44	394582.0
45	400560.5
46	403143.0
47	401889.0
48	399106.5
49	393625.0
50	385345.5
51	373615.0
52	354630.5
53	333011.5
54	312785.5
55	289796.5
56	266758.5
57	242244.0
58	215720.5
59	186859.0
60	156415.0
61	127947.5
62	101396.5
63	78816.0
64	60790.5
65	47398.0
66	37049.0
67	27619.0
68	20311.5
69	15217.0
70	10853.5
71	7259.0
72	4909.0
73	3253.5
74	2042.0
75	1264.0
76	774.0
77	451.5
78	282.0
79	181.0
80	120.0
81	72.5
82	48.5
83	39.0
84	26.5
85	16.0
86	10.5
87	7.0
88	4.0
89	2.0
90	1.5
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.5
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0031781408891179133
4	0.0
5	0.03953891572595896
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.5230707136347827E-5
12-13	7.107663330295654E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	4.196005266616011E-5
46-47	8.723430611215888E-4
48-49	2.131463670240829E-5
50-51	6.441554727207404E-5
52-53	0.0013022745007682068
54-55	6.147196636254001E-4
56-57	0.0036767866703715816
58-59	0.008375425320605704
60-61	0.010964209005748729
62-63	0.0061636756216655215
64-65	0.008196102708004752
66-67	0.005143119240374942
68-69	0.002449628720617435
70-71	0.00450170963706673
72-73	0.0029349467021264246
74-75	0.001258886177512128
76-77	0.0019282629234554376
78-79	6.645692621269521E-4
80-81	4.2912260504256535E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	2.37038631531135E-4
94-95	2.848960236349741E-4
96-97	9.176926087660752E-5
98-99	4.5964098659234E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	107.0
20-21	404.0
22-23	745.0
24-25	1139.0
26-27	2599.0
28-29	5871.0
30-31	16131.0
32-33	20781.0
34-35	29084.0
36-37	47038.0
38-39	55233.0
40-41	54978.0
42-43	63292.0
44-45	74572.0
46-47	75259.0
48-49	70575.0
50-51	62680.0
52-53	60828.0
54-55	65482.0
56-57	68310.0
58-59	68389.0
60-61	65788.0
62-63	64562.0
64-65	68353.0
66-67	72362.0
68-69	74233.0
70-71	73257.0
72-73	69449.0
74-75	71184.0
76-77	75135.0
78-79	78243.0
80-81	78407.0
82-83	74794.0
84-85	74419.0
86-87	76548.0
88-89	80277.0
90-91	83275.0
92-93	81426.0
94-95	88412.0
96-97	145031.0
98-99	833487.0
100-101	6676386.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.60238452186877
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.48322384004203	75.70494111571689
2	7.283699220194658	12.324366443370884
3	1.808496850353069	4.590094378204767
4	0.6339998919476632	2.1455161058151777
5	0.30563624225271596	1.2928777445441653
6	0.16076000346718486	0.8160407777440638
7	0.09628108732787095	0.5701926700607316
8	0.05648014422364703	0.38226839035676724
9	0.03891016408616939	0.29627033974443995
>10	0.1297040260956661	1.6374886147815384
>50	0.0022991309465058567	0.1273247796004985
>100	4.497342904085569E-4	0.07486143494336836
>500	3.9777018199860214E-5	0.020309146802367888
>1k	1.9887754205480673E-5	0.017448058314255225
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.0769023787826094E-5	0.0	0.0	0.0
3	0.0	5.0769023787826094E-5	0.0	0.0	0.0
4	0.0	1.8276848563617394E-4	0.0	0.0	0.0
5	0.0	2.0307609515130438E-4	0.0	0.0	0.0
6	4.061521903026088E-5	2.0307609515130438E-4	0.0	0.0	0.0
7	5.0769023787826094E-5	2.0307609515130438E-4	0.0	0.0	0.0
8	5.0769023787826094E-5	2.0307609515130438E-4	0.0	0.0	0.0
9	5.0769023787826094E-5	3.046141427269566E-4	0.0	0.0	1.015380475756522E-5
10-11	5.58459261666087E-5	3.046141427269566E-4	0.0	2.030760951513044E-5	1.015380475756522E-5
12-13	7.107663330295654E-5	3.046141427269566E-4	0.0	2.030760951513044E-5	1.015380475756522E-5
14-15	7.107663330295654E-5	4.264597998177392E-4	0.0	2.030760951513044E-5	1.015380475756522E-5
16-17	1.1169185233321742E-4	4.366136045753044E-4	0.0	3.046141427269566E-5	1.523070713634783E-5
18-19	1.1676875471200003E-4	4.7722882360556526E-4	0.0	3.046141427269566E-5	3.046141427269566E-5
20-21	1.2184565709078263E-4	5.178440426358261E-4	0.0	3.046141427269566E-5	3.046141427269566E-5
22-23	1.2184565709078263E-4	5.381516521509567E-4	0.0	5.0769023787826094E-5	4.061521903026088E-5
24-25	1.472301689846957E-4	6.193820902114784E-4	0.0	6.599973092417393E-5	5.0769023787826094E-5
26-27	1.624608761210435E-4	6.549204068629567E-4	0.0	1.0153804757565219E-4	5.0769023787826094E-5
28-29	1.624608761210435E-4	6.599973092417393E-4	0.0	2.538451189391305E-4	5.0769023787826094E-5
30-31	1.6753777849982612E-4	7.41227747302261E-4	0.0	7.513815520598262E-4	5.0769023787826094E-5
32-33	1.8784538801495655E-4	7.41227747302261E-4	0.0	0.0019952226348615657	5.0769023787826094E-5
34-35	2.183068022876522E-4	8.732272091506088E-4	0.0	0.0037518308579203487	5.0769023787826094E-5
36-37	2.2338370466643483E-4	0.0010052266709989566	0.0	0.006376589387750958	5.0769023787826094E-5
38-39	2.2846060704521744E-4	0.0010610725971655654	0.0	0.00996088246717148	5.0769023787826094E-5
40-41	2.5384511893913046E-4	0.0012286103756653916	0.0	0.01410363480825809	5.0769023787826094E-5
42-43	2.6907582607547833E-4	0.0012895332042107829	0.0	0.01821084883269322	5.0769023787826094E-5
44-45	2.7922963083304355E-4	0.0014418402755742611	0.0	0.023130367237733568	5.0769023787826094E-5
46-47	2.8430653321182616E-4	0.001533224518392348	0.0	0.028516960661621918	5.0769023787826094E-5
48-49	2.8430653321182616E-4	0.0015383014207711308	0.0	0.03407616876638887	5.0769023787826094E-5
50-51	2.8430653321182616E-4	0.001675377784998261	0.0	0.039097225219004876	5.0769023787826094E-5
52-53	3.046141427269566E-4	0.0016956853945133915	0.0	0.04395582079549983	5.58459261666087E-5
54-55	3.046141427269566E-4	0.0017109161016497392	0.0	0.04912918431947931	6.092282854539132E-5
56-57	3.046141427269566E-4	0.0017363006135436524	0.0	0.054632546498079665	6.092282854539132E-5
58-59	3.046141427269566E-4	0.0017616851254375655	0.0	0.06000390921483166	6.092282854539132E-5
60-61	3.4015245937843485E-4	0.0018073772468466091	0.0	0.06514173442215968	7.107663330295654E-5
62-63	3.5538316651478267E-4	0.0018276848563617395	0.0	0.07004094521768488	7.107663330295654E-5
64-65	3.5538316651478267E-4	0.0019596843182100874	0.0	0.07511784759646749	7.107663330295654E-5
66-67	3.5538316651478267E-4	0.00196476122058887	0.0	0.08036228775374993	7.107663330295654E-5
68-69	3.655369712723479E-4	0.0019952226348615657	0.0	0.08540872871825983	7.107663330295654E-5
70-71	3.807676784086957E-4	0.002040914756270609	0.0	0.09049578490180002	7.107663330295654E-5
72-73	4.0615219030260875E-4	0.0020561454634069566	0.0	0.09436946141681116	8.123043806052176E-5
74-75	4.1630599506017397E-4	0.0020866068776796524	0.0	0.09739529523456558	8.123043806052176E-5
76-77	4.1630599506017397E-4	0.0021729142181189567	0.0	0.10024343746906263	8.123043806052176E-5
78-79	4.1630599506017397E-4	0.002213529437149218	0.0	0.10297988785122644	8.123043806052176E-5
80-81	4.1630599506017397E-4	0.002238913949043131	0.0	0.10403080664363445	9.646114519686958E-5
82-83	4.1630599506017397E-4	0.0022744522656946093	0.0	0.10443188193155828	1.1676875471200003E-4
84-85	4.1630599506017397E-4	0.002299836777588522	0.0	0.1048329572194821	1.2184565709078263E-4
86-87	4.1630599506017397E-4	0.0023455288989975656	0.0	0.10505634092414853	1.2184565709078263E-4
88-89	4.264597998177392E-4	0.002492759067982261	0.0	0.10511218685031515	1.2184565709078263E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13500	0.0	49.739086	1
GTATCAA	24420	0.0	35.872677	1
TCAACGC	29850	0.0	30.847454	4
ATCAACG	30100	0.0	30.549116	3
TATCAAC	30380	0.0	29.981068	2
CAACGCA	30705	0.0	29.903454	5
AACGCAG	31745	0.0	28.95197	6
ACGCAGA	37000	0.0	24.863518	7
CGCAGAG	37740	0.0	24.306847	8
GTGGTAT	6845	0.0	21.234335	1
GCAGAGT	43535	0.0	21.031366	9
TGGTATC	6620	0.0	20.904259	2
GAGTACT	25770	0.0	20.127872	12-13
CAGAGTA	42945	0.0	18.889486	10-11
GTACTTT	28670	0.0	17.993443	14-15
AGAGTAC	39925	0.0	17.676395	10-11
AGTACTT	28575	0.0	15.617627	12-13
ACTTTTT	33360	0.0	15.431662	16-17
TACATGG	27675	0.0	14.167812	2
TATACTG	5730	0.0	14.049638	5
>>END_MODULE