FastQCFastQC Report
Fri 27 May 2016
SRR2140164_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140164_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17858328
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA247400.053.3183331
GTATCAA455600.035.517241
TCAACGC576200.029.6304554
ATCAACG575750.029.6172713
TATCAAC577200.029.3487512
CAACGCA597600.028.547765
AACGCAG617250.027.6291966
TGGTATC110600.024.469072
ACGCAGA724800.023.5115877
CGCAGAG748250.022.774748
GTGGTAT119600.022.4115621
GCAGAGT849850.020.1077659
GAGTACT463500.020.06070712-13
CAGAGTA835100.018.63430610-11
GTACTTT506200.018.34320414-15
GGACCGA31450.016.9841926
AGAGTAC789950.016.85283710-11
TACATGG611400.015.851742
ACTTTTT588750.015.75731316-17
ACCGAAC29000.015.7453298
Produced by FastQC (version 0.11.5)