##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2140164_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17858328 Sequences flagged as poor quality 0 Sequence length 19-101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.50639135981823 18.0 18.0 32.0 18.0 33.0 2 27.907980019182087 29.0 25.0 32.0 18.0 33.0 3 29.897944645209787 31.0 29.0 33.0 25.0 33.0 4 30.533098955288537 32.0 31.0 33.0 27.0 33.0 5 31.449730288300227 33.0 32.0 33.0 28.0 33.0 6 35.70763909140878 37.0 36.0 38.0 31.0 38.0 7 36.04758446591416 38.0 36.0 38.0 33.0 38.0 8 36.57118113185064 38.0 37.0 38.0 34.0 38.0 9 36.73854080852362 38.0 38.0 38.0 34.0 38.0 10-11 36.815231302728904 38.0 38.0 38.0 35.0 38.0 12-13 36.846554615863255 38.0 38.0 38.0 35.0 38.0 14-15 36.869614921396895 38.0 38.0 38.0 35.0 38.0 16-17 36.86027644917262 38.0 38.0 38.0 35.0 38.0 18-19 36.86864612969366 38.0 38.0 38.0 35.0 38.0 20-21 36.87034628996162 38.0 38.0 38.0 35.0 38.0 22-23 36.87848703405312 38.0 38.0 38.0 35.0 38.0 24-25 36.873481821013385 38.0 38.0 38.0 35.0 38.0 26-27 36.82717027921379 38.0 38.0 38.0 35.0 38.0 28-29 36.80948653917207 38.0 38.0 38.0 35.0 38.0 30-31 36.7993102042103 38.0 38.0 38.0 35.0 38.0 32-33 36.7808200936268 38.0 38.0 38.0 35.0 38.0 34-35 36.750837418554866 38.0 38.0 38.0 35.0 38.0 36-37 36.724251006256395 38.0 38.0 38.0 34.5 38.0 38-39 36.70971369999103 38.0 38.0 38.0 34.0 38.0 40-41 36.699692676374006 38.0 38.0 38.0 34.0 38.0 42-43 36.65648480729936 38.0 38.0 38.0 34.0 38.0 44-45 36.59227263619799 38.0 38.0 38.0 34.0 38.0 46-47 36.542997534150146 38.0 38.0 38.0 34.0 38.0 48-49 36.50954793339902 38.0 38.0 38.0 34.0 38.0 50-51 36.494992760939844 38.0 38.0 38.0 34.0 38.0 52-53 36.461762952165586 38.0 38.0 38.0 34.0 38.0 54-55 36.43377614661941 38.0 38.0 38.0 34.0 38.0 56-57 36.40636155500044 38.0 38.0 38.0 34.0 38.0 58-59 36.38705638105857 38.0 38.0 38.0 34.0 38.0 60-61 36.394080104047575 38.0 38.0 38.0 34.0 38.0 62-63 36.378951577355906 38.0 38.0 38.0 34.0 38.0 64-65 36.371343676549834 38.0 38.0 38.0 34.0 38.0 66-67 36.34624186849156 38.0 38.0 38.0 33.5 38.0 68-69 36.33834068486419 38.0 38.0 38.0 33.5 38.0 70-71 36.329004277571165 38.0 38.0 38.0 33.0 38.0 72-73 36.30993957747102 38.0 37.0 38.0 33.0 38.0 74-75 36.30272256403852 38.0 37.0 38.0 33.0 38.0 76-77 36.28306203329268 38.0 37.0 38.0 33.0 38.0 78-79 36.29562022569955 38.0 37.0 38.0 33.0 38.0 80-81 36.254488959658666 38.0 37.0 38.0 33.0 38.0 82-83 36.25694682777305 38.0 37.0 38.0 33.0 38.0 84-85 36.24293625441515 38.0 37.0 38.0 33.0 38.0 86-87 36.206277687310106 38.0 37.0 38.0 33.0 38.0 88-89 36.19809565905112 38.0 37.0 38.0 33.0 38.0 90-91 36.20080869100829 38.0 37.0 38.0 33.0 38.0 92-93 36.18269206414215 38.0 37.0 38.0 33.0 38.0 94-95 36.1623906524576 38.0 37.0 38.0 33.0 38.0 96-97 36.17346259044969 38.0 37.0 38.0 33.0 38.0 98-99 36.207248592501344 38.0 37.0 38.0 33.0 38.0 100-101 35.62994621819326 38.0 36.0 38.0 30.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 13.0 18 52.0 19 84.0 20 214.0 21 865.0 22 3581.0 23 10638.0 24 24993.0 25 47084.0 26 79319.0 27 120752.0 28 174759.0 29 234582.0 30 305734.0 31 389162.0 32 497473.0 33 657333.0 34 929167.0 35 1512940.0 36 3655446.0 37 9214137.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.08866809927559 25.63434269994369 14.097915549540808 23.179073651239914 2 14.659659067746992 20.510710745149265 39.457540481953295 25.372089705150447 3 18.259904155662547 24.99222464520359 31.00421185117506 25.743659347958804 4 13.877855754469287 17.89765536840851 37.70762862010374 30.516860257018465 5 14.272007562833197 36.48598709655446 34.6029924301646 14.639012910447748 6 30.60354810371945 37.7057135472033 17.955040359881394 13.735697989195852 7 26.916926377430183 32.70067612152717 22.405188212468715 17.97720928857394 8 24.33743517310243 37.545692967449135 20.139858557867232 17.977013301581202 9 25.221762082094134 17.286153552561025 21.179787939834007 36.31229642551083 10-11 24.58310202199243 26.97833492344131 27.972126278801273 20.466436775764983 12-13 25.199170671433375 24.317919475211024 28.597898053241565 21.885011800114032 14-15 22.39521247453849 25.618952121385608 26.32232703979902 25.663508364276883 16-17 21.816625274213802 28.830302590477675 27.085449992854876 22.26762214245365 18-19 21.88269528927904 27.815910313664304 28.980087049582693 21.321307347473965 20-21 22.785649906964895 26.80239709336596 28.99386324185719 21.41808975781195 22-23 22.432470247244325 26.692289434309917 28.893672616886473 21.98156770155929 24-25 22.132691877214576 26.90106364682062 29.015655556366372 21.95058891959843 26-27 22.092892799438264 27.013510596336378 28.91827626191898 21.97532034230638 28-29 22.07063457901288 27.045425994209126 28.956998095670134 21.92694133110786 30-31 22.323629045553766 26.90892766638052 28.837825681437934 21.92961760662778 32-33 22.10080400378454 27.066165313230044 28.769179710695305 22.063850972290115 34-35 22.181545101756868 27.117619825950463 28.846146912648152 21.85468815964452 36-37 22.2194388869627 26.956272763249032 28.78581180589222 22.038476543896042 38-39 22.05608748057688 27.149754961998905 28.832810482845634 21.96134707457858 40-41 22.331100240387926 27.009177550310138 28.670239420740177 21.989482788561755 42-43 22.161409974186974 27.14379284536035 28.472780157351295 22.22201702310138 44-45 22.201472496654077 27.175291552595592 28.407958797964284 22.215277152786044 46-47 22.32488589050567 27.16413535671776 28.225191766948036 22.285786985828537 48-49 22.24418029891791 27.333059106838554 28.154010397052154 22.26875019719138 50-51 22.210852865475534 27.38640751205178 28.056012452851082 22.346727169621605 52-53 22.362627196206862 27.354239363344053 27.929648149206933 22.353485291242155 54-55 22.392050728118313 27.286768050937365 27.976612887253427 22.344568333690894 56-57 22.353074946900893 27.3837805953349 27.93298424212125 22.330160215642955 58-59 22.275725571564 27.467803176386614 28.02238288973853 22.23408836231086 60-61 22.346949716410535 27.405000583234145 28.03758173572343 22.21046796463189 62-63 22.430487370773186 27.412416766454484 27.958577264034034 22.198518598738293 64-65 22.285210584558214 27.394095566558924 28.007759796943805 22.312934051939056 66-67 22.38536634352373 27.48855239093314 27.835209887513596 22.290871378029532 68-69 22.34411192927226 27.350659914430075 27.934400498933233 22.370827657364433 70-71 22.319248501933366 27.151371322852235 27.969904379608117 22.559475795606275 72-73 22.392705453589844 27.240073740187032 27.896717813271575 22.470502992951545 74-75 22.199731517417657 27.34762778507282 27.930425804979947 22.522214892529576 76-77 22.349521505145862 27.303757551726004 27.83068664633631 22.51603429679182 78-79 22.27394150690973 27.379389664523046 27.811428896290337 22.535239932276895 80-81 22.374088243613517 27.35110429087941 27.762797151228437 22.512010314278644 82-83 22.395878481884917 27.341462556862272 27.895090553083296 22.36756840816951 84-85 22.488030394567062 27.343435396595783 27.756793378447362 22.411740830389796 86-87 22.341776158166446 27.41526871383973 27.785173952004342 22.45778117598948 88-89 22.41379481230488 27.35049515072147 27.77500259465463 22.460707442319023 90-91 22.416995824601027 27.430252224165052 27.775201387385035 22.37755056384888 92-93 22.512542979078006 27.374096455467473 27.734149307341966 22.37921125811256 94-95 22.309445967073852 27.542410463665863 27.750647867196214 22.39749570206407 96-97 22.361538498351337 27.523822359802303 27.777919369412935 22.33671977243343 98-99 22.624300661314763 28.092674041409467 27.77130320836727 21.5117220889085 100-101 23.223149772495404 30.618560661105636 25.124374548762646 21.03391501763631 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5553.0 1 5207.0 2 5555.0 3 6478.0 4 7589.5 5 9971.5 6 13269.0 7 18391.5 8 23659.0 9 26710.5 10 26338.5 11 25063.5 12 26856.0 13 32989.5 14 46579.5 15 62702.0 16 74116.0 17 79448.5 18 78949.5 19 73880.0 20 66743.5 21 61322.5 22 61274.0 23 69158.5 24 84853.5 25 109208.0 26 140523.0 27 174506.0 28 210983.0 29 249337.5 30 284569.0 31 320908.5 32 359861.5 33 400360.0 34 440589.5 35 478220.5 36 522459.5 37 558545.0 38 580985.0 39 601890.5 40 624787.5 41 646307.0 42 660153.0 43 676043.5 44 694662.5 45 710374.0 46 719919.0 47 726164.0 48 731269.5 49 731701.0 50 724518.5 51 710544.5 52 684467.5 53 651601.0 54 623989.5 55 589494.0 56 547505.0 57 499837.5 58 446888.0 59 392280.0 60 334787.0 61 277327.5 62 220879.5 63 173652.0 64 136789.0 65 106526.0 66 81846.5 67 59387.0 68 42646.5 69 32284.5 70 23341.0 71 15453.0 72 10477.5 73 6836.5 74 4287.5 75 2698.5 76 1699.0 77 1027.5 78 657.5 79 439.0 80 291.0 81 193.0 82 133.5 83 91.0 84 57.5 85 42.0 86 31.5 87 21.5 88 15.0 89 8.5 90 7.5 91 7.0 92 4.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0034829688423238725 4 0.0 5 0.03817266655646598 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.1199256727729494E-5 12-13 1.4559033746048343E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 3.774142207878417E-5 46-47 0.0010221257122078242 48-49 2.3633418267899685E-5 50-51 8.042008114445757E-5 52-53 0.0015186256282000495 54-55 8.10366081970404E-4 56-57 0.0040023730139709945 58-59 0.00880993271037334 60-61 0.01106813107445076 62-63 0.00642735843883715 64-65 0.008472355504575734 66-67 0.005548679679746107 68-69 0.002808217878937952 70-71 0.004565704456603886 72-73 0.002992878323370592 74-75 0.0014619569432229385 76-77 0.0020984788127086677 78-79 7.837949420198624E-4 80-81 4.112697007108814E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 3.5794246225255125E-6 92-93 2.3171700344440082E-4 94-95 2.709891337751775E-4 96-97 1.335535037038282E-4 98-99 6.240766154740065E-4 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 169.0 20-21 597.0 22-23 1332.0 24-25 2148.0 26-27 4909.0 28-29 11873.0 30-31 32363.0 32-33 41077.0 34-35 57595.0 36-37 94456.0 38-39 115931.0 40-41 111875.0 42-43 125477.0 44-45 148217.0 46-47 148920.0 48-49 141764.0 50-51 129550.0 52-53 122301.0 54-55 130899.0 56-57 136497.0 58-59 138407.0 60-61 135381.0 62-63 130015.0 64-65 136303.0 66-67 144196.0 68-69 147555.0 70-71 148346.0 72-73 135669.0 74-75 137796.0 76-77 145355.0 78-79 151691.0 80-81 154377.0 82-83 144778.0 84-85 140993.0 86-87 146278.0 88-89 154989.0 90-91 159430.0 92-93 155668.0 94-95 164953.0 96-97 265261.0 98-99 1491518.0 100-101 1.1771419E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.73181356159861 #Duplication Level Percentage of deduplicated Percentage of total 1 87.28277317901684 70.46496571427686 2 8.457363284339696 13.655565517880433 3 2.2374860409902095 5.41908917723703 4 0.8795039274418649 2.8401578838772155 5 0.41880504223950593 1.6905445294368615 6 0.2275422855494484 1.102194082461486 7 0.14438981834673778 0.8159796326473348 8 0.08902371813904297 0.5749636972289217 9 0.05657979975137838 0.41110108603927764 >10 0.20023400947675896 2.4767589173836155 >50 0.004476403193307559 0.24883934460909896 >100 0.0017377987574160596 0.2297497288794009 >500 5.646684067391203E-5 0.03353987380946464 >1k 2.8225917078403853E-5 0.036550814232852016 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.599628363864747E-6 2 0.0 3.919739854705323E-5 0.0 5.599628363864747E-6 5.599628363864747E-6 3 0.0 3.919739854705323E-5 0.0 5.599628363864747E-6 5.599628363864747E-6 4 0.0 1.2879145236888918E-4 0.0 1.1199256727729494E-5 5.599628363864747E-6 5 0.0 1.3439108073275393E-4 0.0 1.1199256727729494E-5 5.599628363864747E-6 6 0.0 1.4559033746048343E-4 0.0 1.1199256727729494E-5 5.599628363864747E-6 7 0.0 1.4559033746048343E-4 0.0 1.1199256727729494E-5 5.599628363864747E-6 8 0.0 1.567895941882129E-4 0.0 1.1199256727729494E-5 5.599628363864747E-6 9 0.0 3.135791883764258E-4 0.0 1.679888509159424E-5 1.1199256727729494E-5 10-11 0.0 3.135791883764258E-4 0.0 2.239851345545899E-5 1.1199256727729494E-5 12-13 0.0 3.135791883764258E-4 0.0 2.239851345545899E-5 1.1199256727729494E-5 14-15 0.0 4.0317324219826177E-4 0.0 2.239851345545899E-5 2.5198327637391364E-5 16-17 0.0 4.2557175565372077E-4 0.0 2.239851345545899E-5 2.7998141819323736E-5 18-19 0.0 4.7316859674657115E-4 0.0 2.5198327637391364E-5 3.919739854705323E-5 20-21 0.0 5.235652520213538E-4 0.0 3.919739854705323E-5 4.19972127289856E-5 22-23 0.0 5.963604207515956E-4 0.0 3.919739854705323E-5 5.5996283638647465E-5 24-25 0.0 6.607561469360401E-4 0.0 6.719554036637697E-5 6.439572618444459E-5 26-27 0.0 6.775550320276344E-4 0.0 1.2039200982309206E-4 6.719554036637697E-5 28-29 0.0 6.887542887553639E-4 0.0 4.535698974730445E-4 6.719554036637697E-5 30-31 0.0 7.811481567591321E-4 0.0 0.0014223056044216458 6.999535454830934E-5 32-33 0.0 7.923474134868616E-4 0.0 0.003524966055052858 7.839479709410645E-5 34-35 0.0 9.267384942196156E-4 0.0 0.0063555781929864885 8.39944254579712E-5 36-37 0.0 0.001063929389134302 0.0 0.01058049779352244 8.39944254579712E-5 38-39 2.7998141819323736E-6 0.001125525301136814 0.0 0.01776762079854284 9.239386800376833E-5 40-41 5.599628363864747E-6 0.0012543167535057033 0.0 0.0255819021803161 9.51936821857007E-5 42-43 5.599628363864747E-6 0.0013299117364178772 0.0 0.03257303819260123 9.799349636763308E-5 44-45 5.599628363864747E-6 0.0015762953844279263 0.0 0.040922084083123575 1.0079331054956544E-4 46-47 5.599628363864747E-6 0.0017526836778896657 0.0 0.04977509652639374 1.0079331054956544E-4 48-49 5.599628363864747E-6 0.0017582833062535306 0.0 0.05898928499913318 1.0079331054956544E-4 50-51 5.599628363864747E-6 0.001985068254990053 0.0 0.06812787848896044 1.0079331054956544E-4 52-53 5.599628363864747E-6 0.0020046669542635796 0.0 0.07680730245295081 1.0359312473149782E-4 54-55 5.599628363864747E-6 0.0020186660251732417 0.0 0.08562111749767393 1.0919275309536257E-4 56-57 5.599628363864747E-6 0.0020550636095383623 0.0 0.092365869861949 1.1199256727729494E-4 58-59 5.599628363864747E-6 0.0020662628662660915 0.0 0.09815308577600322 1.1199256727729494E-4 60-61 5.599628363864747E-6 0.002119459335722807 0.0 0.1037219161838667 1.1199256727729494E-4 62-63 5.599628363864747E-6 0.002192254504453048 0.0 0.10933554361864112 1.1199256727729494E-4 64-65 5.599628363864747E-6 0.0023994407539160443 0.0 0.11458239539558238 1.1199256727729494E-4 66-67 5.599628363864747E-6 0.002416239639007638 0.0 0.12006443156380597 1.1199256727729494E-4 68-69 5.599628363864747E-6 0.0024638364801004885 0.0 0.12591884301822656 1.1199256727729494E-4 70-71 5.599628363864747E-6 0.0025086335070114065 0.0 0.13152407101045516 1.175921956411597E-4 72-73 5.599628363864747E-6 0.0025366316488307306 0.0 0.13572939191171762 1.3439108073275393E-4 74-75 5.599628363864747E-6 0.0026150264459248364 0.0 0.13920116149731376 1.3439108073275393E-4 76-77 5.599628363864747E-6 0.00271301994229247 0.0 0.14240134910726243 1.371908949146863E-4 78-79 5.599628363864747E-6 0.002738218269929861 0.0 0.14515076663392004 1.3999070909661868E-4 80-81 5.599628363864747E-6 0.002738218269929861 0.0 0.1463854846881522 1.3999070909661868E-4 82-83 5.599628363864747E-6 0.002743817898293726 0.0 0.14682505551471559 1.4559033746048343E-4 84-85 5.599628363864747E-6 0.002766216411749185 0.0 0.14720023061509455 1.4559033746048343E-4 86-87 5.599628363864747E-6 0.0028054138102962386 0.0 0.14740181723619367 1.4559033746048343E-4 88-89 5.599628363864747E-6 0.003015399873941166 0.0 0.14746061333401425 1.4559033746048343E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 24740 0.0 53.318333 1 GTATCAA 45560 0.0 35.51724 1 TCAACGC 57620 0.0 29.630455 4 ATCAACG 57575 0.0 29.617271 3 TATCAAC 57720 0.0 29.348751 2 CAACGCA 59760 0.0 28.54776 5 AACGCAG 61725 0.0 27.629196 6 TGGTATC 11060 0.0 24.46907 2 ACGCAGA 72480 0.0 23.511587 7 CGCAGAG 74825 0.0 22.77474 8 GTGGTAT 11960 0.0 22.411562 1 GCAGAGT 84985 0.0 20.107765 9 GAGTACT 46350 0.0 20.060707 12-13 CAGAGTA 83510 0.0 18.634306 10-11 GTACTTT 50620 0.0 18.343204 14-15 GGACCGA 3145 0.0 16.984192 6 AGAGTAC 78995 0.0 16.852837 10-11 TACATGG 61140 0.0 15.85174 2 ACTTTTT 58875 0.0 15.757313 16-17 ACCGAAC 2900 0.0 15.745329 8 >>END_MODULE