FastQCFastQC Report
Fri 27 May 2016
SRR2140163_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140163_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9918365
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145700.053.2961241
GTATCAA257800.035.4674571
ATCAACG322400.029.3991763
TCAACGC324300.029.2256054
TATCAAC323600.029.1961062
CAACGCA333650.028.4066055
AACGCAG345400.027.455466
TGGTATC64150.024.3316462
GTGGTAT67900.024.0123791
ACGCAGA404750.023.4188257
CGCAGAG412700.022.9361088
GAGTACT267100.019.89167212-13
GCAGAGT477500.019.8235249
CAGAGTA470100.018.72571210-11
GTACTTT289450.018.28857614-15
AGAGTAC443000.016.82506410-11
GTACATG334400.016.6164511
TACATGG333650.016.4843982
ACATGGG340000.015.9719243
ACTTTTT338400.015.50856916-17
Produced by FastQC (version 0.11.5)