##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140163_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9918365
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.913857576324325	18.0	18.0	32.0	18.0	33.0
2	28.27881762770376	31.0	27.0	32.0	18.0	33.0
3	30.128931532566103	31.0	29.0	33.0	27.0	33.0
4	30.67595818464031	32.0	31.0	33.0	27.0	33.0
5	31.52395863632766	33.0	32.0	33.0	28.0	33.0
6	35.75769353114147	37.0	36.0	38.0	31.0	38.0
7	36.069784384825525	38.0	37.0	38.0	33.0	38.0
8	36.56839166536017	38.0	37.0	38.0	34.0	38.0
9	36.728820526366995	38.0	38.0	38.0	34.0	38.0
10-11	36.79952996285174	38.0	38.0	38.0	34.5	38.0
12-13	36.83299898723227	38.0	38.0	38.0	35.0	38.0
14-15	36.85391115370325	38.0	38.0	38.0	35.0	38.0
16-17	36.84560282869203	38.0	38.0	38.0	35.0	38.0
18-19	36.852873129795086	38.0	38.0	38.0	35.0	38.0
20-21	36.85634720350485	38.0	38.0	38.0	35.0	38.0
22-23	36.864570802067334	38.0	38.0	38.0	35.0	38.0
24-25	36.85982781726584	38.0	38.0	38.0	35.0	38.0
26-27	36.81364213622482	38.0	38.0	38.0	35.0	38.0
28-29	36.79236524029737	38.0	38.0	38.0	35.0	38.0
30-31	36.78174246317954	38.0	38.0	38.0	35.0	38.0
32-33	36.76200235716324	38.0	38.0	38.0	35.0	38.0
34-35	36.73286828576737	38.0	38.0	38.0	34.5	38.0
36-37	36.70420543099747	38.0	38.0	38.0	34.0	38.0
38-39	36.689486970464415	38.0	38.0	38.0	34.0	38.0
40-41	36.67774924310527	38.0	38.0	38.0	34.0	38.0
42-43	36.63213746748412	38.0	38.0	38.0	34.0	38.0
44-45	36.56682561441876	38.0	38.0	38.0	34.0	38.0
46-47	36.51427582119482	38.0	38.0	38.0	34.0	38.0
48-49	36.4794659917592	38.0	38.0	38.0	34.0	38.0
50-51	36.46595072924511	38.0	38.0	38.0	34.0	38.0
52-53	36.4324863563089	38.0	38.0	38.0	34.0	38.0
54-55	36.40548344696953	38.0	38.0	38.0	34.0	38.0
56-57	36.37577419975568	38.0	38.0	38.0	34.0	38.0
58-59	36.35817545083866	38.0	38.0	38.0	34.0	38.0
60-61	36.36194613449285	38.0	38.0	38.0	34.0	38.0
62-63	36.346018296182635	38.0	38.0	38.0	33.5	38.0
64-65	36.3364673118691	38.0	38.0	38.0	33.0	38.0
66-67	36.31020015054184	38.0	38.0	38.0	33.0	38.0
68-69	36.30537756698099	38.0	38.0	38.0	33.0	38.0
70-71	36.29510220910666	38.0	37.0	38.0	33.0	38.0
72-73	36.27336513259141	38.0	37.0	38.0	33.0	38.0
74-75	36.26817511348362	38.0	37.0	38.0	33.0	38.0
76-77	36.247261238967	38.0	37.0	38.0	33.0	38.0
78-79	36.26087395697459	38.0	37.0	38.0	33.0	38.0
80-81	36.21680958903774	38.0	37.0	38.0	33.0	38.0
82-83	36.218911245519664	38.0	37.0	38.0	33.0	38.0
84-85	36.204645136142105	38.0	37.0	38.0	33.0	38.0
86-87	36.169441591975314	38.0	37.0	38.0	33.0	38.0
88-89	36.158789922186585	38.0	37.0	38.0	33.0	38.0
90-91	36.16184295964527	38.0	37.0	38.0	33.0	38.0
92-93	36.14420557964012	38.0	37.0	38.0	33.0	38.0
94-95	36.123541452418294	38.0	37.0	38.0	33.0	38.0
96-97	36.135296732526456	38.0	37.0	38.0	33.0	38.0
98-99	36.172094170288474	38.0	37.0	38.0	33.0	38.0
100-101	35.57524422345803	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	9.0
18	34.0
19	65.0
20	104.0
21	502.0
22	2161.0
23	6458.0
24	14466.0
25	27477.0
26	46165.0
27	70427.0
28	100626.0
29	135380.0
30	173343.0
31	220817.0
32	279716.0
33	368784.0
34	516979.0
35	832139.0
36	1947402.0
37	5175311.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	37.752331155387004	24.904407127586047	14.350328910057254	22.992932806969694
2	14.667719931662123	20.641648094217143	39.66367440601349	25.026957568107242
3	18.27963841126162	25.123331217317023	31.025262330903548	25.57176804051781
4	13.728028762805161	18.081881439128324	37.944217620545324	30.245872177521193
5	14.126199451955756	36.682674152516384	34.728329982022345	14.462796413505513
6	30.324100796855124	37.876898057290695	18.107984531724735	13.691016614129445
7	26.767062918132172	32.8507067445088	22.43066271507451	17.95156762228452
8	24.281774264205843	37.590379059451834	20.211143671361157	17.916703004981162
9	25.151655540000796	17.428769761951692	21.326871918910022	36.09270277913749
10-11	24.47291329865053	27.091868532545714	28.009504793809985	20.425713374993766
12-13	25.137922309802263	24.433864061436395	28.63735323864929	21.790860390112048
14-15	22.272602389607563	25.801581208193085	26.426638866385737	25.499177535813615
16-17	21.686038979206753	28.947926397143075	27.209716520817693	22.156318102832472
18-19	21.6471022011744	27.98425083087035	29.149856309475215	21.218790658480035
20-21	22.547342502660896	26.92799194771256	29.220026405058068	21.304639144568473
22-23	22.274589660820425	26.86658316982583	29.050462653262603	21.80836451609114
24-25	21.98147764220346	27.054736780966156	29.14831095893229	21.815474617898094
26-27	21.91547910274259	27.14205631011788	29.12324317362847	21.819221413511062
28-29	21.848532892827656	27.2205503001868	29.127131599709834	21.80378520727571
30-31	22.111121348470604	27.05157735972889	29.06944674083844	21.767854550962067
32-33	21.902409042435266	27.19753261979471	28.984475761101574	21.915582576668452
34-35	22.016246886587794	27.243034052981667	29.02755108479403	21.713167975636512
36-37	22.025488724744584	27.133810287781024	29.02709540395776	21.813605583516633
38-39	21.912433397288154	27.26569592387289	29.00256083379493	21.819309845044025
40-41	22.149145684204573	27.18927596848807	28.837176779679968	21.824401567627387
42-43	21.984499995448598	27.275090428071717	28.71827259419125	22.02213698228843
44-45	22.087742609013407	27.34373238097475	28.58432530262991	21.984199707381926
46-47	22.161363625982254	27.335917662278053	28.320778950829233	22.181939760910456
48-49	22.132409879882182	27.429754510186793	28.300223418889036	22.137612191041992
50-51	22.070382576083354	27.567087486797327	28.177131024087952	22.185398913031364
52-53	22.216683271886236	27.528376189902797	28.059789741898573	22.19515079631239
54-55	22.181212201562698	27.49214919934345	28.11283577194626	22.213802827147592
56-57	22.180589789271536	27.531037636596494	28.10664496275538	22.181727611376587
58-59	22.174039499166405	27.622048676405342	28.111339410825824	22.09257241360243
60-61	22.23823250064406	27.54167039834413	28.17032813330989	22.049768967701915
62-63	22.25458323251848	27.561605115022502	28.115240175509186	22.068571476949824
64-65	22.16394802615583	27.549270840879554	28.133096433238748	22.153684699725876
66-67	22.228249655887648	27.566544225111468	28.014744343299697	22.19046177570119
68-69	22.16653441827209	27.51580706731162	28.07371429692601	22.24394421749028
70-71	22.13840132089696	27.329073685735544	28.131665768236253	22.400859225131242
72-73	22.227768558170975	27.39617671113781	28.08364705078132	22.2924076799099
74-75	22.06957645411368	27.512568870201083	28.075751838878833	22.342102836806408
76-77	22.18574267118582	27.466889320161048	28.038273791106462	22.309094217546672
78-79	22.11949130541444	27.49249748129729	27.985543956423463	22.402467256864806
80-81	22.194926217524618	27.467565270592655	27.98575985390011	22.35174865798262
82-83	22.191552930466507	27.51751300861868	28.082464630280995	22.20846943063382
84-85	22.355371711002814	27.521433587346287	27.917388775798013	22.205805925852882
86-87	22.17907777318518	27.590219956953216	27.957164114128563	22.273538155733043
88-89	22.24624782808412	27.56391549000419	27.90538685067205	22.284449831239638
90-91	22.283648736190937	27.600885686966425	27.938570711401766	22.176894865440875
92-93	22.335549275321767	27.516963100400766	27.924597593428746	22.22289003084872
94-95	22.140085087009446	27.679101636186076	27.95439412559915	22.226419151205324
96-97	22.15764942288418	27.7284231895326	27.925971225681334	22.187956161901887
98-99	22.47439822862257	28.207845078694422	27.96808309812493	21.34967359455808
100-101	23.07436948845686	30.71088040813146	25.350940296986373	20.863809806425312
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3191.0
1	2884.0
2	2912.5
3	3399.0
4	3979.5
5	5269.5
6	7260.5
7	10305.5
8	13209.5
9	14938.0
10	14617.0
11	13733.5
12	14790.0
13	18324.0
14	26053.5
15	35307.0
16	41792.0
17	44882.5
18	44945.5
19	42290.5
20	38405.0
21	35319.0
22	34953.5
23	38716.0
24	46942.5
25	60032.0
26	76706.0
27	95231.5
28	115826.5
29	137637.0
30	158668.0
31	179767.5
32	201518.0
33	224546.0
34	247265.5
35	268128.5
36	290268.0
37	311538.0
38	328815.5
39	343606.5
40	357817.5
41	369914.0
42	376897.0
43	383920.5
44	392127.5
45	399132.5
46	404375.5
47	406201.5
48	406680.5
49	404797.0
50	398535.5
51	388104.0
52	372781.5
53	354378.5
54	335445.0
55	312690.5
56	287538.0
57	260148.5
58	230694.5
59	200296.5
60	168185.0
61	137778.0
62	109603.5
63	84882.0
64	64493.0
65	48835.0
66	37065.0
67	27814.0
68	20536.0
69	14937.5
70	10612.5
71	7211.5
72	4923.0
73	3247.0
74	2128.0
75	1329.5
76	817.0
77	511.0
78	339.0
79	223.0
80	137.5
81	81.5
82	55.0
83	36.0
84	23.5
85	16.5
86	10.5
87	7.5
88	6.0
89	3.0
90	2.0
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.003599383567755371
4	0.0
5	0.03868581162318588
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.5123460368720046E-5
12-13	9.578191566856029E-5
14-15	0.0
16-17	0.0
18-19	5.041153456240016E-6
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	3.1250362959944795E-5
46-47	9.923069435339548E-4
48-49	1.0584558716516409E-5
50-51	1.1197164579337455E-4
52-53	0.0014762760823089946
54-55	7.296355475844524E-4
56-57	0.003914172592909065
58-59	0.008485548923687379
60-61	0.010707385614082937
62-63	0.006265723833961325
64-65	0.008192033382199372
66-67	0.005508661550209753
68-69	0.002731968820421986
70-71	0.004476129199959796
72-73	0.0028027489686841254
74-75	0.0013982116814092195
76-77	0.001964759298400385
78-79	8.038348280195386E-4
80-81	3.967737484869243E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	2.485686588063733E-4
94-95	2.576552764354475E-4
96-97	1.1084638386493955E-4
98-99	6.117711628956352E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	109.0
20-21	368.0
22-23	689.0
24-25	1154.0
26-27	2607.0
28-29	5901.0
30-31	15696.0
32-33	20002.0
34-35	28140.0
36-37	47125.0
38-39	57902.0
40-41	56216.0
42-43	64157.0
44-45	75410.0
46-47	76770.0
48-49	72386.0
50-51	64434.0
52-53	61819.0
54-55	65749.0
56-57	69040.0
58-59	69920.0
60-61	68823.0
62-63	66123.0
64-65	68909.0
66-67	73656.0
68-69	75455.0
70-71	75577.0
72-73	70205.0
74-75	71134.0
76-77	75864.0
78-79	79470.0
80-81	81008.0
82-83	75059.0
84-85	73631.0
86-87	76427.0
88-89	81522.0
90-91	84512.0
92-93	82118.0
94-95	88032.0
96-97	145225.0
98-99	838280.0
100-101	6711741.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.95730265561859
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.67858917631582	71.8590066954096
2	8.27913202000036	13.57070657377985
3	2.1706438803605463	5.337003523808269
4	0.8508401887961651	2.789302674589239
5	0.3957864031390073	1.6218793014521147
6	0.2086469639042143	1.0260085421324174
7	0.13305168775546258	0.7633190209550716
8	0.07642838760014155	0.5011091595220583
9	0.04877180626575252	0.3597485118465127
>10	0.15476524783469064	1.8821403079023733
>50	0.0025665363695678844	0.14064138319336683
>100	6.906373728310149E-4	0.0926922359373229
>500	6.529844939391268E-5	0.036694637331035215
>1k	2.1765836001711033E-5	0.019747432140804832
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.0246920737440092E-5	0.0	1.008230691248003E-5	0.0
3	0.0	3.0246920737440092E-5	0.0	2.016461382496006E-5	0.0
4	0.0	1.008230691248003E-4	0.0	2.016461382496006E-5	0.0
5	0.0	1.008230691248003E-4	0.0	3.0246920737440092E-5	0.0
6	8.065845529984024E-5	1.1090537603728034E-4	0.0	4.032922764992012E-5	0.0
7	8.065845529984024E-5	1.1090537603728034E-4	0.0	4.032922764992012E-5	0.0
8	8.065845529984024E-5	1.1090537603728034E-4	0.0	4.032922764992012E-5	0.0
9	8.065845529984024E-5	1.6131691059968048E-4	0.0	7.057614838736022E-5	0.0
10-11	8.065845529984024E-5	1.6131691059968048E-4	0.0	9.074076221232028E-5	0.0
12-13	8.065845529984024E-5	1.6131691059968048E-4	0.0	9.074076221232028E-5	0.0
14-15	8.065845529984024E-5	1.9156383133712057E-4	0.0	1.008230691248003E-4	0.0
16-17	1.2098768294976037E-4	1.9156383133712057E-4	0.0	1.1090537603728034E-4	0.0
18-19	1.2098768294976037E-4	2.318930589870407E-4	0.0	1.1090537603728034E-4	0.0
20-21	1.2098768294976037E-4	2.7726344009320085E-4	0.0	1.2098768294976037E-4	0.0
22-23	1.2098768294976037E-4	3.528807419368011E-4	0.0	1.361111433184804E-4	0.0
24-25	1.2602883640600037E-4	3.730453557617611E-4	0.0	1.6131691059968048E-4	0.0
26-27	1.310699898622404E-4	3.8312766267424114E-4	0.0	1.8148152442464053E-4	0.0
28-29	1.310699898622404E-4	3.8312766267424114E-4	0.0	3.932099695867212E-4	0.0
30-31	1.310699898622404E-4	4.032922764992012E-4	0.0	0.0011090537603728034	0.0
32-33	1.310699898622404E-4	4.1337458341168126E-4	0.0	0.002873457470056809	0.0
34-35	1.4115229677472043E-4	4.688272714303214E-4	0.0	0.005066359223521216	0.0
36-37	1.4115229677472043E-4	5.242799594489616E-4	0.0	0.008358232430445946	0.0
38-39	1.4115229677472043E-4	5.444445732739217E-4	0.0	0.01322294551571756	0.0
40-41	1.4115229677472043E-4	5.646091870988817E-4	0.0	0.018314510506519978	0.0
42-43	1.4619345023096045E-4	5.898149543800818E-4	0.0	0.02303303014156063	0.0
44-45	1.5123460368720047E-4	6.755145631361622E-4	0.0	0.028573257789968408	0.0
46-47	1.5123460368720047E-4	7.360084046110423E-4	0.0	0.03488982307063715	0.0
48-49	1.5123460368720047E-4	7.511318649797622E-4	0.0	0.04095433067849388	0.0
50-51	1.613169105996805E-4	9.074076221232028E-4	0.0	0.046978509058700706	0.0
52-53	1.7139921751216052E-4	9.275722359481628E-4	0.0	0.05310855166148856	0.0
54-55	1.7139921751216052E-4	9.376545428606428E-4	0.0	0.0593999111748761	0.0
56-57	1.7139921751216052E-4	9.578191566856029E-4	0.0	0.0655854064656826	0.0
58-59	1.7139921751216052E-4	9.578191566856029E-4	0.0	0.07140289755418358	0.0
60-61	2.016461382496006E-4	9.83024923966803E-4	0.0	0.07770938052793984	0.0
62-63	2.4197536589952074E-4	9.88066077423043E-4	0.0	0.08322440240906641	0.0
64-65	2.4197536589952074E-4	0.0011342595276540035	0.0	0.08856802507268083	0.0
66-67	2.4701651935576073E-4	0.0011594652949352036	0.0	0.09421411694366964	0.0
68-69	2.5205767281200075E-4	0.0011796299087601637	0.0	0.09980475612663983	0.0
70-71	2.5205767281200075E-4	0.0012098768294976038	0.0	0.1050677203349544	0.0
72-73	2.621399797244808E-4	0.0012149179829538438	0.0	0.10903006695155906	0.0
74-75	2.621399797244808E-4	0.0012653295175162438	0.0	0.11212029402023418	0.0
76-77	2.621399797244808E-4	0.001345987972816084	0.0	0.11479210535204139	0.0
78-79	2.621399797244808E-4	0.0013611114331848041	0.0	0.11755969859951715	0.0
80-81	2.621399797244808E-4	0.0013611114331848041	0.0	0.11876957542901476	0.0
82-83	2.621399797244808E-4	0.0013611114331848041	0.0	0.11917286770551397	0.0
84-85	2.621399797244808E-4	0.0013611114331848041	0.0	0.11954591306127572	0.0
86-87	2.621399797244808E-4	0.0013812760470097642	0.0	0.11976268265989404	0.0
88-89	2.621399797244808E-4	0.0014720168092220846	0.0	0.11983829996173764	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14570	0.0	53.296124	1
GTATCAA	25780	0.0	35.467457	1
ATCAACG	32240	0.0	29.399176	3
TCAACGC	32430	0.0	29.225605	4
TATCAAC	32360	0.0	29.196106	2
CAACGCA	33365	0.0	28.406605	5
AACGCAG	34540	0.0	27.45546	6
TGGTATC	6415	0.0	24.331646	2
GTGGTAT	6790	0.0	24.012379	1
ACGCAGA	40475	0.0	23.418825	7
CGCAGAG	41270	0.0	22.936108	8
GAGTACT	26710	0.0	19.891672	12-13
GCAGAGT	47750	0.0	19.823524	9
CAGAGTA	47010	0.0	18.725712	10-11
GTACTTT	28945	0.0	18.288576	14-15
AGAGTAC	44300	0.0	16.825064	10-11
GTACATG	33440	0.0	16.616451	1
TACATGG	33365	0.0	16.484398	2
ACATGGG	34000	0.0	15.971924	3
ACTTTTT	33840	0.0	15.508569	16-17
>>END_MODULE