FastQCFastQC Report
Fri 27 May 2016
SRR2140162_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140162_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8687296
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113050.053.3655661
GTATCAA211550.036.2077561
TCAACGC262550.031.045444
ATCAACG265100.030.6494273
TATCAAC263350.030.5392822
CAACGCA272700.029.8580155
AACGCAG283600.028.7316866
ACGCAGA337000.024.2047567
CGCAGAG346250.023.545578
TGGTATC55000.023.092742
GTGGTAT55250.022.2797031
GCAGAGT397150.020.5826389
GAGTACT220100.019.93282712-13
CAGAGTA389800.018.73423410-11
GTACTTT239400.018.1625214-15
AGAGTAC364250.017.01034410-11
GTACATG283450.015.91321
TACATGG280600.015.9043152
ACATGGG284450.015.4902613
ACTTTTT282250.015.46754116-17
Produced by FastQC (version 0.11.5)