##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140162_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8687296
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.416251731263674	18.0	18.0	32.0	18.0	33.0
2	27.808951139687196	29.0	25.0	32.0	18.0	33.0
3	29.837806608638637	31.0	29.0	33.0	25.0	33.0
4	30.499649833504005	32.0	31.0	33.0	27.0	33.0
5	31.43871890632022	33.0	32.0	33.0	28.0	33.0
6	35.717341621604696	37.0	36.0	38.0	31.0	38.0
7	36.050474163652304	38.0	37.0	38.0	33.0	38.0
8	36.56844730512233	38.0	37.0	38.0	34.0	38.0
9	36.730998575391006	38.0	38.0	38.0	34.0	38.0
10-11	36.806936473673744	38.0	38.0	38.0	34.5	38.0
12-13	36.840002976760545	38.0	38.0	38.0	35.0	38.0
14-15	36.865027506832966	38.0	38.0	38.0	35.0	38.0
16-17	36.852394864869346	38.0	38.0	38.0	35.0	38.0
18-19	36.861635024292944	38.0	38.0	38.0	35.0	38.0
20-21	36.86227167691151	38.0	38.0	38.0	35.0	38.0
22-23	36.870176897023214	38.0	38.0	38.0	35.0	38.0
24-25	36.86560054581962	38.0	38.0	38.0	35.0	38.0
26-27	36.8167328997346	38.0	38.0	38.0	35.0	38.0
28-29	36.79622135657974	38.0	38.0	38.0	35.0	38.0
30-31	36.786700271887064	38.0	38.0	38.0	35.0	38.0
32-33	36.7687210502301	38.0	38.0	38.0	35.0	38.0
34-35	36.73860153598312	38.0	38.0	38.0	34.5	38.0
36-37	36.71195960270316	38.0	38.0	38.0	34.0	38.0
38-39	36.70130991481949	38.0	38.0	38.0	34.0	38.0
40-41	36.689196848303446	38.0	38.0	38.0	34.0	38.0
42-43	36.64247928022618	38.0	38.0	38.0	34.0	38.0
44-45	36.57707293687628	38.0	38.0	38.0	34.0	38.0
46-47	36.530387566478666	38.0	38.0	38.0	34.0	38.0
48-49	36.496083902595885	38.0	38.0	38.0	34.0	38.0
50-51	36.48214747726111	38.0	38.0	38.0	34.0	38.0
52-53	36.44785190145956	38.0	38.0	38.0	34.0	38.0
54-55	36.41968013605607	38.0	38.0	38.0	34.0	38.0
56-57	36.390716383147755	38.0	38.0	38.0	34.0	38.0
58-59	36.37208014314122	38.0	38.0	38.0	34.0	38.0
60-61	36.37586716463804	38.0	38.0	38.0	34.0	38.0
62-63	36.3633276940927	38.0	38.0	38.0	34.0	38.0
64-65	36.35227571954864	38.0	38.0	38.0	34.0	38.0
66-67	36.325532136396674	38.0	38.0	38.0	33.0	38.0
68-69	36.3185832018369	38.0	38.0	38.0	33.0	38.0
70-71	36.308055538782966	38.0	37.0	38.0	33.0	38.0
72-73	36.28596258469972	38.0	37.0	38.0	33.0	38.0
74-75	36.280030235490884	38.0	37.0	38.0	33.0	38.0
76-77	36.256440397420604	38.0	37.0	38.0	33.0	38.0
78-79	36.26980750221557	38.0	37.0	38.0	33.0	38.0
80-81	36.22921133467898	38.0	37.0	38.0	33.0	38.0
82-83	36.228891268732255	38.0	37.0	38.0	33.0	38.0
84-85	36.21481704903165	38.0	37.0	38.0	33.0	38.0
86-87	36.175871364452355	38.0	37.0	38.0	33.0	38.0
88-89	36.16922310408029	38.0	37.0	38.0	33.0	38.0
90-91	36.17149073747174	38.0	37.0	38.0	33.0	38.0
92-93	36.15261740503604	38.0	37.0	38.0	33.0	38.0
94-95	36.12938096304099	38.0	37.0	38.0	33.0	38.0
96-97	36.14066103999198	38.0	37.0	38.0	33.0	38.0
98-99	36.17301419439188	38.0	37.0	38.0	33.0	38.0
100-101	35.57993205588292	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	10.0
18	22.0
19	68.0
20	95.0
21	501.0
22	1924.0
23	5577.0
24	12602.0
25	23741.0
26	39743.0
27	60697.0
28	86503.0
29	116325.0
30	149689.0
31	191535.0
32	243998.0
33	321720.0
34	453536.0
35	737632.0
36	1770608.0
37	4470770.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	36.549842436587866	26.266274338988794	14.017537793117674	23.16634543130567
2	14.662352934676107	20.792096873411474	39.20895523762515	25.33659495428727
3	18.14157957528476	25.174409154598504	31.19022995624288	25.49378131387386
4	13.835156532021012	18.073713615836272	38.00807523998262	30.083054612160105
5	14.221899639558783	36.52530107107501	34.736130336907344	14.516668952458861
6	30.256745021696048	38.043437221432306	18.081449049278394	13.618368707593248
7	26.556974690398484	32.83081409911669	22.685551407480535	17.926659803004295
8	24.09146643558594	37.60431324085193	20.371114326022735	17.933105997539396
9	25.085676832008485	17.2800374247637	21.446466196155857	36.18781954707195
10-11	24.408194702252274	27.1048279640079	28.183748325356262	20.303229008383564
12-13	25.043550710569978	24.450413831752872	28.721229580721207	21.78480587695594
14-15	22.2266514229514	25.78960127524146	26.431694050714977	25.552053251092172
16-17	21.745028602686038	28.905893157088236	27.176753272825056	22.172324967400673
18-19	21.730858485770487	27.987914766574086	29.027369390889866	21.253857356765558
20-21	22.646303871447834	27.017265327681383	29.0109340226119	21.32549677825888
22-23	22.2785822573869	26.912257026011677	28.911040353768552	21.898120362832866
24-25	21.997078578703672	27.107482078146404	29.01904092971562	21.876398413434302
26-27	21.981090402306354	27.16462942313413	28.942043639189386	21.912236535370134
28-29	21.983630872512595	27.22519770359537	28.97636402293272	21.814807400959317
30-31	22.185916302749373	27.098690266596552	28.882470947515916	21.83292248313816
32-33	22.019124807698415	27.228432637439713	28.811785453485385	21.94065710137649
34-35	22.100498817141688	27.229795233093146	28.823243700977212	21.846462248787958
36-37	22.127498551238563	27.093315906602548	28.841446445739134	21.937739096419755
38-39	21.990553243765238	27.29160771995065	28.886046031311913	21.831793004972198
40-41	22.236179077051148	27.204958866936185	28.67338683849885	21.885475217513818
42-43	22.072734141860163	27.28090769777514	28.54793770910685	22.098420451257837
44-45	22.13005213826768	27.343890785112674	28.444720856364302	22.081336220255345
46-47	22.237175450233106	27.3111607046648	28.22630544254664	22.22535840255545
48-49	22.213656302621185	27.43971051200215	28.19230956711935	22.15432361825731
50-51	22.13776785892257	27.53554112400476	28.09167791734507	22.235013099727603
52-53	22.28814076747532	27.537689973212352	27.939168194193492	22.235001065118833
54-55	22.32666381837148	27.44682034362709	27.98113128643271	22.24538455156873
56-57	22.2536670208627	27.515801554181664	28.03242894535386	22.19810247960178
58-59	22.208148515648716	27.601415708882204	28.037044890879837	22.153390884589243
60-61	22.31096250761966	27.487429923698826	28.055316651517643	22.14629091716387
62-63	22.294613568452032	27.533054998170115	28.066894630858087	22.10543680251977
64-65	22.229133340256528	27.481946826780085	28.07170598650181	22.217213846461572
66-67	22.285432128302475	27.57637712663926	27.90180100751852	22.236389737539742
68-69	22.25876156018315	27.474980086439967	27.973360190234214	22.292898163142674
70-71	22.264140130224412	27.267621012714187	28.030254596538605	22.437984260522793
72-73	22.332044106490937	27.33684241643828	27.955480836553313	22.37563264051747
74-75	22.091876081602845	27.465187674305003	28.009328005660706	22.433608238431443
76-77	22.28035328610065	27.467902860445587	27.898844057756893	22.35289979569687
78-79	22.226988014703043	27.54578209154389	27.79237113204757	22.4348587617055
80-81	22.25882650902002	27.461750971952082	27.825636839670114	22.453785679357775
82-83	22.303109492612354	27.457662578893398	27.950142237462604	22.289085691031644
84-85	22.393277202492204	27.449514641200924	27.886436710084002	22.27077144622287
86-87	22.25538309832712	27.52833591694991	27.876412355917214	22.33986862880576
88-89	22.3222874929427	27.50298892232332	27.79639757908501	22.378326005648972
90-91	22.348865318484545	27.561284481713294	27.834172189317023	22.255678010485134
92-93	22.426865857823326	27.495425049834864	27.796178864377207	22.281530227964602
94-95	22.225567896341513	27.620702541600373	27.826358631479188	22.327370930578926
96-97	22.28204874341122	27.62541210439336	27.846150767742834	22.24638838445259
98-99	22.572148898427173	28.187428063607133	27.864404267510594	21.3760187704551
100-101	23.156121712872277	30.710894627117785	25.23497205488003	20.89801160512991
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2170.0
1	2066.0
2	2401.0
3	2947.5
4	3436.0
5	4435.0
6	5851.5
7	8157.5
8	10451.5
9	11900.5
10	11901.0
11	11303.0
12	12215.5
13	15210.0
14	21433.5
15	28854.0
16	34178.5
17	36436.5
18	35668.5
19	33103.5
20	30319.5
21	28183.0
22	28540.5
23	32775.5
24	40901.5
25	53651.5
26	69833.0
27	87573.0
28	105687.0
29	123553.5
30	140843.5
31	158817.5
32	177726.5
33	197961.0
34	218202.5
35	235039.5
36	254854.5
37	273116.0
38	284921.5
39	296152.0
40	309162.0
41	321060.0
42	327242.0
43	332100.0
44	339226.0
45	347155.5
46	351964.5
47	353671.5
48	353820.0
49	352035.0
50	347157.5
51	339904.5
52	326567.5
53	309198.5
54	293433.0
55	274776.0
56	253303.5
57	230142.0
58	205282.5
59	178937.5
60	151428.0
61	124114.5
62	98080.5
63	76245.0
64	58950.5
65	44930.0
66	33763.0
67	24960.0
68	18392.0
69	13591.5
70	9727.5
71	6652.5
72	4623.0
73	3046.0
74	1890.5
75	1156.0
76	709.5
77	417.5
78	251.0
79	163.0
80	109.0
81	69.5
82	45.5
83	33.0
84	25.0
85	21.0
86	17.5
87	11.0
88	6.5
89	4.5
90	3.5
91	2.5
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0029007875407952026
4	0.0
5	0.037031085391818125
6	0.0
7	0.0
8	0.0
9	0.0
10-11	5.755530834911116E-6
12-13	1.0935508586331121E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	1.7818547670762417E-5
46-47	9.697787024863808E-4
48-49	1.2065618140319037E-5
50-51	8.507887845133105E-5
52-53	0.0014927347255001549
54-55	7.329061045042808E-4
56-57	0.004057362167405647
58-59	0.00888271287177607
60-61	0.011552576886052664
62-63	0.006556174707521937
64-65	0.00835701527313277
66-67	0.005748889165734997
68-69	0.0027883081365171264
70-71	0.004668498877625989
72-73	0.0030486574995321203
74-75	0.001360215639519385
76-77	0.0021453453303196274
78-79	8.008277029377142E-4
80-81	4.865218709054645E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	1.9627141021226317E-4
94-95	2.939090148801726E-4
96-97	1.1901649233849927E-4
98-99	5.830443491917049E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	95.0
20-21	265.0
22-23	583.0
24-25	908.0
26-27	2021.0
28-29	4747.0
30-31	13298.0
32-33	17057.0
34-35	24138.0
36-37	39764.0
38-39	48517.0
40-41	47728.0
42-43	54319.0
44-45	64703.0
46-47	65391.0
48-49	62024.0
50-51	55498.0
52-53	53717.0
54-55	57814.0
56-57	60761.0
58-59	61040.0
60-61	60322.0
62-63	58085.0
64-65	60263.0
66-67	64853.0
68-69	67097.0
70-71	66213.0
72-73	61688.0
74-75	63432.0
76-77	66008.0
78-79	70584.0
80-81	70564.0
82-83	66585.0
84-85	65960.0
86-87	67867.0
88-89	72632.0
90-91	74548.0
92-93	72721.0
94-95	77757.0
96-97	128315.0
98-99	738166.0
100-101	5879248.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.38229025402495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.82798575810618	74.06680891162806
2	7.660699586123489	12.775333528780752
3	1.8842146814096656	4.713304063985876
4	0.715118661880616	2.3851292732399783
5	0.3362889700517888	1.402027225504268
6	0.19876737377709552	0.9944207311987228
7	0.10667209848311623	0.6226194714407594
8	0.06879233705164421	0.45888500922343306
9	0.047033117649902256	0.3529556160692268
>10	0.15116392431634942	1.918372724554313
>50	0.002609848053440389	0.14467981560301538
>100	5.576811501852489E-4	0.08872928332764715
>500	4.798136831510477E-5	0.025064911080372215
>1k	4.798057818320226E-5	0.051669434363682924
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.1511061669822232E-5	0.0	1.1511061669822232E-5	0.0
3	0.0	1.1511061669822232E-5	0.0	1.1511061669822232E-5	0.0
4	0.0	9.208849335857786E-5	0.0	1.1511061669822232E-5	0.0
5	0.0	9.208849335857786E-5	0.0	1.1511061669822232E-5	0.0
6	1.2662167836804456E-4	9.208849335857786E-5	0.0	1.1511061669822232E-5	1.1511061669822232E-5
7	1.2662167836804456E-4	9.208849335857786E-5	0.0	1.1511061669822232E-5	1.1511061669822232E-5
8	1.2662167836804456E-4	9.208849335857786E-5	0.0	1.1511061669822232E-5	1.1511061669822232E-5
9	1.2662167836804456E-4	1.6115486337751124E-4	0.0	5.7555308349111164E-5	1.1511061669822232E-5
10-11	1.49643801707689E-4	1.6115486337751124E-4	0.0	6.331083918402228E-5	1.1511061669822232E-5
12-13	1.6115486337751124E-4	1.7266592504733348E-4	0.0	6.90663700189334E-5	1.1511061669822232E-5
14-15	1.6691039421242235E-4	2.1871017172662242E-4	0.0	6.90663700189334E-5	1.7266592504733347E-5
16-17	1.9568804838697795E-4	2.2446570256153355E-4	0.0	9.208849335857786E-5	4.604424667928893E-5
18-19	2.071991100568002E-4	2.705099492408225E-4	0.0	1.035995550284001E-4	4.604424667928893E-5
20-21	2.071991100568002E-4	3.223097267550225E-4	0.0	1.0935508586331121E-4	4.604424667928893E-5
22-23	2.1871017172662242E-4	3.5108738092957806E-4	0.0	1.2662167836804456E-4	5.179977751420005E-5
24-25	2.5324335673608913E-4	3.913760967739559E-4	0.0	1.6115486337751124E-4	6.90663700189334E-5
26-27	2.935320725804669E-4	4.259092817834226E-4	0.0	2.1871017172662242E-4	6.90663700189334E-5
28-29	3.107986650852003E-4	4.259092817834226E-4	0.0	3.741095042692226E-4	6.90663700189334E-5
30-31	3.165541959201114E-4	4.4317587428815595E-4	0.0	0.0010705287352934675	7.482190085384452E-5
32-33	3.223097267550225E-4	4.4893140512306705E-4	0.0	0.003079208996677447	8.057743168875562E-5
34-35	3.338207884248447E-4	4.719535284627115E-4	0.0	0.005703731057396916	8.057743168875562E-5
36-37	3.4533185009466696E-4	4.949756518023559E-4	0.0	0.009749869234339431	8.057743168875562E-5
38-39	3.4533185009466696E-4	5.064867134721783E-4	0.0	0.015643532809288416	8.057743168875562E-5
40-41	3.568429117644892E-4	5.295088368118227E-4	0.0	0.02238325941696933	8.057743168875562E-5
42-43	3.568429117644892E-4	5.46775429316556E-4	0.0	0.02892154244542836	8.057743168875562E-5
44-45	3.6835397343431143E-4	6.388639226751339E-4	0.0	0.03625408872910512	8.057743168875562E-5
46-47	3.798650351041337E-4	6.906637001893339E-4	0.0	0.04448449782302802	8.633296252366674E-5
48-49	3.798650351041337E-4	6.964192310242451E-4	0.0	0.05269188479361127	9.208849335857786E-5
50-51	4.028871584437781E-4	7.712411318780896E-4	0.0	0.06172806820442173	9.208849335857786E-5
52-53	4.143982201136004E-4	7.712411318780896E-4	0.0	0.069998766014189	1.2662167836804456E-4
54-55	4.143982201136004E-4	7.712411318780896E-4	0.0	0.07835004125564501	1.6115486337751124E-4
56-57	4.143982201136004E-4	7.712411318780896E-4	0.0	0.08453723690317447	1.6115486337751124E-4
58-59	4.143982201136004E-4	7.827521935479118E-4	0.0	0.08939490492783946	1.6115486337751124E-4
60-61	4.374203434532448E-4	8.115298477224674E-4	0.0	0.09459790480259911	1.6115486337751124E-4
62-63	4.4893140512306705E-4	8.40307501897023E-4	0.0	0.09917930734718836	1.6115486337751124E-4
64-65	4.4893140512306705E-4	9.43907056925423E-4	0.0	0.10382402073096163	1.6115486337751124E-4
66-67	4.4893140512306705E-4	9.496625877603341E-4	0.0	0.10840542327555087	1.6691039421242235E-4
68-69	4.604424667928893E-4	9.726847110999785E-4	0.0	0.1131307140910129	1.7266592504733348E-4
70-71	4.8346459013253373E-4	9.957068344396231E-4	0.0	0.11828191418825834	1.8417698671715572E-4
72-73	5.410198984816449E-4	0.0010072178961094452	0.0	0.12200574263844584	1.9568804838697795E-4
74-75	5.410198984816449E-4	0.001035995550284001	0.0	0.12506192951178363	1.9568804838697795E-4
76-77	5.410198984816449E-4	0.0010993063894680231	0.0	0.1280317834225978	1.9568804838697795E-4
78-79	5.410198984816449E-4	0.001139595105312401	0.0	0.13074263844584091	1.9568804838697795E-4
80-81	5.525309601514672E-4	0.001139595105312401	0.0	0.13194554439033734	2.0144357922188908E-4
82-83	5.640420218212894E-4	0.001139595105312401	0.0	0.13247505322714917	2.1871017172662242E-4
84-85	5.697975526562005E-4	0.0011683727594869566	0.0	0.1329182291014373	2.1871017172662242E-4
86-87	5.870641451609338E-4	0.0011741282903218677	0.0	0.13313693927316395	2.1871017172662242E-4
88-89	5.98575206830756E-4	0.0012547057220106234	0.0	0.1332290277665225	2.1871017172662242E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11305	0.0	53.365566	1
GTATCAA	21155	0.0	36.207756	1
TCAACGC	26255	0.0	31.04544	4
ATCAACG	26510	0.0	30.649427	3
TATCAAC	26335	0.0	30.539282	2
CAACGCA	27270	0.0	29.858015	5
AACGCAG	28360	0.0	28.731686	6
ACGCAGA	33700	0.0	24.204756	7
CGCAGAG	34625	0.0	23.54557	8
TGGTATC	5500	0.0	23.09274	2
GTGGTAT	5525	0.0	22.279703	1
GCAGAGT	39715	0.0	20.582638	9
GAGTACT	22010	0.0	19.932827	12-13
CAGAGTA	38980	0.0	18.734234	10-11
GTACTTT	23940	0.0	18.16252	14-15
AGAGTAC	36425	0.0	17.010344	10-11
GTACATG	28345	0.0	15.9132	1
TACATGG	28060	0.0	15.904315	2
ACATGGG	28445	0.0	15.490261	3
ACTTTTT	28225	0.0	15.467541	16-17
>>END_MODULE