FastQCFastQC Report
Fri 27 May 2016
SRR2140161_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140161_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8399343
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132650.055.9824141
GTATCAA255100.040.3943141
TCAACGC301050.033.7090264
ATCAACG302150.033.6020553
TATCAAC307950.033.1803442
CAACGCA310450.032.6883625
AACGCAG318350.032.001736
GTGGTAT59700.029.626981
TGGTATC56500.029.2333132
ACGCAGA360550.028.2801747
CGCAGAG393150.025.8910968
GCAGAGT425900.023.9001739
GAGTACT254000.022.34073612-13
GTACATG300850.021.6864261
TACATGG300050.021.2530162
CAGAGTA412600.021.24149110-11
ACATGGG300350.020.6400133
GTACTTT282100.020.0850714-15
CATGGGG233100.019.2478184
AGAGTAC388100.018.858710-11
Produced by FastQC (version 0.11.5)