##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140161_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8399343
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.83685854953179	31.0	18.0	33.0	18.0	33.0
2	30.082494785604066	32.0	28.0	33.0	25.0	33.0
3	30.770017369215665	33.0	30.0	33.0	27.0	33.0
4	31.41410524608889	33.0	31.0	33.0	28.0	34.0
5	31.674476920397225	33.0	32.0	33.0	30.0	34.0
6	34.55483065758834	37.0	34.0	38.0	28.0	38.0
7	35.68355048722263	38.0	36.0	38.0	31.0	38.0
8	35.613380713229596	38.0	36.0	38.0	31.0	38.0
9	36.28066290422953	38.0	37.0	38.0	33.0	38.0
10-11	36.56003529085549	38.0	37.5	38.0	34.0	38.0
12-13	36.66649861780856	38.0	38.0	38.0	34.0	38.0
14-15	36.680041879466046	38.0	38.0	38.0	34.0	38.0
16-17	36.685141683105456	38.0	38.0	38.0	34.0	38.0
18-19	36.7107464238572	38.0	38.0	38.0	34.0	38.0
20-21	36.73259724777593	38.0	38.0	38.0	34.0	38.0
22-23	36.751265155848124	38.0	38.0	38.0	34.0	38.0
24-25	36.75271821470833	38.0	38.0	38.0	34.0	38.0
26-27	36.70844488994729	38.0	38.0	38.0	34.0	38.0
28-29	36.693794942844974	38.0	38.0	38.0	34.0	38.0
30-31	36.68556823611328	38.0	38.0	38.0	34.0	38.0
32-33	36.66752437038199	38.0	38.0	38.0	34.0	38.0
34-35	36.634708839003466	38.0	38.0	38.0	34.0	38.0
36-37	36.604725147402796	38.0	38.0	38.0	34.0	38.0
38-39	36.59035285934624	38.0	38.0	38.0	34.0	38.0
40-41	36.5769137354325	38.0	38.0	38.0	34.0	38.0
42-43	36.5386200131335	38.0	38.0	38.0	34.0	38.0
44-45	36.466196407782434	38.0	38.0	38.0	34.0	38.0
46-47	36.41052449980567	38.0	38.0	38.0	34.0	38.0
48-49	36.36800139390114	38.0	38.0	38.0	34.0	38.0
50-51	36.349699206222645	38.0	38.0	38.0	33.5	38.0
52-53	36.3112102801654	38.0	38.0	38.0	33.0	38.0
54-55	36.27628781896572	38.0	38.0	38.0	33.0	38.0
56-57	36.244449159026914	38.0	37.5	38.0	33.0	38.0
58-59	36.21974730391375	38.0	37.0	38.0	33.0	38.0
60-61	36.224826746477156	38.0	37.0	38.0	33.0	38.0
62-63	36.211557705048136	38.0	37.0	38.0	33.0	38.0
64-65	36.20566211103453	38.0	37.0	38.0	33.0	38.0
66-67	36.17884836243543	38.0	37.0	38.0	33.0	38.0
68-69	36.17157148773474	38.0	37.0	38.0	33.0	38.0
70-71	36.16035124175397	38.0	37.0	38.0	33.0	38.0
72-73	36.141877347570755	38.0	37.0	38.0	33.0	38.0
74-75	36.13590705139087	38.0	37.0	38.0	33.0	38.0
76-77	36.11031413269865	38.0	37.0	38.0	33.0	38.0
78-79	36.12490884909356	38.0	37.0	38.0	33.0	38.0
80-81	36.07516986009722	38.0	37.0	38.0	33.0	38.0
82-83	36.07293309376939	38.0	37.0	38.0	33.0	38.0
84-85	36.05968756471922	38.0	37.0	38.0	33.0	38.0
86-87	36.02377080220233	38.0	37.0	38.0	32.0	38.0
88-89	36.017016317882984	38.0	37.0	38.0	32.0	38.0
90-91	36.01798785278142	38.0	37.0	38.0	32.0	38.0
92-93	36.000043787201854	38.0	37.0	38.0	31.5	38.0
94-95	35.97793016663374	38.0	37.0	38.0	31.0	38.0
96-97	35.98859658997365	38.0	37.0	38.0	31.5	38.0
98-99	36.0385225100631	38.0	37.0	38.0	33.0	38.0
100-101	35.4716533507324	38.0	35.5	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	7.0
19	35.0
20	102.0
21	532.0
22	2171.0
23	6616.0
24	14780.0
25	27760.0
26	45509.0
27	67407.0
28	94295.0
29	126150.0
30	162315.0
31	203601.0
32	257298.0
33	331243.0
34	455276.0
35	704089.0
36	1519230.0
37	4380926.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.27805496215597	17.75638880326711	13.888014812587127	21.07754142198979
2	15.701799533606378	20.21359289649202	41.996141841094	22.0884657288076
3	19.53864828197116	25.945292394405772	28.625547162375586	25.890512161247482
4	13.293706424419149	18.308872491574636	37.018121536410646	31.37929954759557
5	15.00805367017255	36.747139053368606	34.09955155754313	14.145255718915713
6	31.141864310101397	36.57183663055551	18.06644876867155	14.219850290671543
7	27.759790259785795	32.43845381716165	21.72628263901117	18.075473284041383
8	25.099105965788038	36.37430927633268	19.8806263775631	18.645958380316173
9	25.91982491963955	17.314568532324493	20.892145969035912	35.87346057900005
10-11	25.22773062296245	26.626357955994767	27.20879044996677	20.93712097107601
12-13	25.898708373475415	24.078748167116146	27.7232225134094	22.29932094599904
14-15	22.881742060063505	25.15083620230773	25.964822486711164	26.0025992509176
16-17	22.294178247036704	28.43724205571793	26.423382162152443	22.84519753509292
18-19	22.29555335458976	27.27063890592395	28.51548627077142	21.918321468714876
20-21	23.29955545692166	26.249966365788303	28.66485514191494	21.785623035375096
22-23	23.07049628872902	26.133789115986445	28.46718674467837	22.32852785060616
24-25	22.720617641337846	26.28932039840753	28.690627823069942	22.299434137184683
26-27	22.52826073286158	26.340380989312635	28.743174501241953	22.388183776583833
28-29	22.61866274255187	26.41540107828565	28.626569948245788	22.339366230916692
30-31	22.941598670585964	26.099493658492552	28.56793608518477	22.390971585736715
32-33	22.683547141032793	26.227457476272026	28.541838877442522	22.547156505252662
34-35	22.896156475790256	26.36741243081577	28.460023052078693	22.276408041315285
36-37	22.965075537009564	26.182367013009678	28.36885110135251	22.483706348628246
38-39	22.598793894558735	26.496923103602732	28.455299093979797	22.44898390785873
40-41	22.90575995776041	26.3183038722544	28.342685471583078	22.43325069840211
42-43	22.73110926183585	26.416793131654696	28.096716606883216	22.755380999626233
44-45	22.61791194543535	26.492277547065058	28.08362539795557	22.806185109544028
46-47	22.82372538244462	26.475735737669297	27.858920442842845	22.841618437043234
48-49	22.926660638759653	26.708333398626955	27.56733835519625	22.797667607417146
50-51	22.85427107741468	26.792925097179783	27.571051954935623	22.78175187046992
52-53	23.16748292823069	26.706779328702318	27.359941259194315	22.76579648387268
54-55	23.102048268426827	26.60014149035267	27.401480572155695	22.89632966906481
56-57	23.0619240687258	26.823875827012944	27.290279932699413	22.82392017156184
58-59	22.903208115548104	26.732444376946844	27.550567223229816	22.813780284275236
60-61	22.99523090066076	26.708114022766328	27.444072657649986	22.852582418922925
62-63	22.919778849627118	26.767852278900538	27.564072442557507	22.748296428914834
64-65	22.968262415339353	26.69104284885738	27.510451560407063	22.83024317539621
66-67	23.014389619034894	26.858764882940566	27.161458202531552	22.965387295492985
68-69	22.955892766811576	26.84106587272111	27.295004506212184	22.90803685425513
70-71	22.99327735264018	26.595868437188376	27.271529000639344	23.139325209532103
72-73	22.96014929599869	26.67970382985188	27.1388919544241	23.22125491972533
74-75	22.79604503942983	26.72096535190403	27.263639935486655	23.219349673179483
76-77	23.05571768332917	26.779042685597716	27.021440822366838	23.143798808706283
78-79	22.939236805684164	26.758035763512396	27.114129966113865	23.188597464689575
80-81	23.0007806073545	26.676453172625596	27.136328997942606	23.186437222077295
82-83	23.165591803536753	26.622720265094685	27.192879791642916	23.018808139725643
84-85	23.180984673878974	26.72948462600646	27.08405430308361	23.005476397030957
86-87	23.035878901703548	26.871774390522756	27.041542342950674	23.050804364823023
88-89	23.186318234707546	26.78942099814709	26.906795272998256	23.11746549414711
90-91	23.14823869427314	26.852662594344263	26.94801893006526	23.051079781317338
92-93	23.249305592109334	26.88561108767871	26.985994414354515	22.87908890585744
94-95	23.027212049972793	27.085883993993825	27.009085640882446	22.877818315150936
96-97	23.040783388410592	27.01104158978834	27.046097716578455	22.90207730522261
98-99	23.359541288878322	27.54580664394531	27.130602316703	21.96404975047337
100-101	24.015370452442124	29.99668579923202	24.464353924502127	21.523589823823734
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2613.0
1	2313.0
2	2612.5
3	3351.5
4	3856.5
5	5537.0
6	7508.0
7	10219.0
8	13690.0
9	15235.5
10	14126.5
11	12554.5
12	12986.5
13	15793.0
14	21976.0
15	29817.0
16	36658.5
17	41711.0
18	43194.5
19	40725.5
20	36199.0
21	31377.0
22	28640.0
23	29860.0
24	34653.5
25	43601.5
26	55929.0
27	69420.5
28	84062.5
29	99206.0
30	112946.0
31	127149.5
32	143336.5
33	160175.5
34	176046.5
35	190978.5
36	209514.0
37	226197.0
38	238240.0
39	250291.0
40	263162.0
41	275240.0
42	284110.5
43	292499.5
44	302540.0
45	316631.5
46	331794.0
47	347292.5
48	357122.5
49	360325.5
50	359314.5
51	357497.0
52	343079.5
53	321403.0
54	309759.0
55	296692.5
56	281360.0
57	261239.0
58	237510.0
59	210197.5
60	180181.5
61	152743.5
62	125519.5
63	104261.0
64	87172.0
65	68528.5
66	53154.0
67	37464.5
68	24473.0
69	18667.0
70	13936.5
71	9389.5
72	6612.0
73	4442.0
74	2869.0
75	1855.5
76	1158.5
77	675.0
78	398.0
79	242.5
80	152.0
81	98.5
82	67.0
83	47.5
84	32.0
85	23.0
86	17.0
87	11.5
88	7.0
89	4.0
90	3.0
91	2.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0039050673368143203
4	0.0
5	0.03775295282023844
6	0.0
7	0.0
8	0.0
9	0.0
10-11	5.952846550021829E-6
12-13	1.3096262410048024E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	2.4645497621863514E-5
46-47	9.943939796405292E-4
48-49	6.26818714500122E-6
50-51	9.476322618244764E-5
52-53	0.0014059018742834909
54-55	8.258123592998187E-4
56-57	0.003996006056398341
58-59	0.008910187387795237
60-61	0.011575889997914379
62-63	0.006583384328751148
64-65	0.008619648322332878
66-67	0.005718954631345403
68-69	0.0028173589307170224
70-71	0.004582111436950147
72-73	0.0028689834338566874
74-75	0.0014225375891679522
76-77	0.0018274048981032729
78-79	7.351130420080348E-4
80-81	4.7118934186842854E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	1.8229384446096736E-4
94-95	2.2282614082566E-4
96-97	1.1680170760981812E-4
98-99	4.90954090400101E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	85.0
20-21	353.0
22-23	790.0
24-25	1211.0
26-27	2526.0
28-29	5488.0
30-31	14854.0
32-33	18971.0
34-35	25948.0
36-37	41581.0
38-39	48149.0
40-41	49743.0
42-43	57610.0
44-45	69152.0
46-47	69765.0
48-49	64178.0
50-51	57065.0
52-53	48536.0
54-55	52087.0
56-57	51857.0
58-59	59617.0
60-61	58426.0
62-63	57192.0
64-65	61313.0
66-67	65718.0
68-69	67171.0
70-71	67159.0
72-73	61923.0
74-75	63279.0
76-77	66506.0
78-79	69594.0
80-81	70997.0
82-83	66871.0
84-85	66172.0
86-87	68603.0
88-89	73133.0
90-91	74805.0
92-93	74233.0
94-95	80656.0
96-97	130819.0
98-99	734768.0
100-101	5580439.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.58842820996098
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.02250415072261	76.14862129634315
2	6.976137036550865	11.802009337982659
3	1.6330978723570821	4.144235464071512
4	0.5896699047315688	1.9951700161584336
5	0.278305837164124	1.1770726663685298
6	0.15512059394582933	0.7872844334923976
7	0.08722108498544487	0.5164526139980345
8	0.057301651422072296	0.38776453021025376
9	0.037194942705521365	0.28316355667376447
>10	0.15700843849084997	2.1760197850292515
>50	0.004659913353136001	0.26831726237422315
>100	0.0017025683470931789	0.2391607394565137
>500	5.06701492938885E-5	0.03979651542628375
>1k	2.5335074563275703E-5	0.03493178241500138
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.952846550021829E-5	0.0	0.0	0.0
3	0.0	5.952846550021829E-5	0.0	0.0	0.0
4	0.0	1.666797034006112E-4	0.0	0.0	0.0
5	0.0	1.666797034006112E-4	0.0	0.0	0.0
6	2.3811386200087318E-5	1.666797034006112E-4	0.0	0.0	0.0
7	2.3811386200087318E-5	1.666797034006112E-4	0.0	0.0	0.0
8	2.3811386200087318E-5	2.262081689008295E-4	0.0	1.1905693100043659E-5	1.1905693100043659E-5
9	3.571707930013097E-5	3.4526509990126606E-4	0.0	1.1905693100043659E-5	1.1905693100043659E-5
10-11	3.571707930013097E-5	3.4526509990126606E-4	0.0	1.1905693100043659E-5	1.1905693100043659E-5
12-13	5.952846550021829E-5	3.4526509990126606E-4	0.0	1.1905693100043659E-5	1.1905693100043659E-5
14-15	6.548131205024012E-5	3.9288787230144074E-4	0.0	1.1905693100043659E-5	1.785853965006549E-5
16-17	1.1905693100043658E-4	3.9288787230144074E-4	0.0	1.1905693100043659E-5	3.571707930013097E-5
18-19	1.250097775504584E-4	4.047935654014844E-4	0.0	1.1905693100043659E-5	5.952846550021829E-5
20-21	1.3096262410048024E-4	4.3455779815159356E-4	0.0	1.1905693100043659E-5	7.143415860026195E-5
22-23	1.3096262410048024E-4	4.8813341710179E-4	0.0	1.785853965006549E-5	7.143415860026195E-5
24-25	2.262081689008295E-4	5.476618826020083E-4	0.0	6.548131205024012E-5	7.143415860026195E-5
26-27	3.095480206011351E-4	5.714732688020956E-4	0.0	1.7263254995063303E-4	7.143415860026195E-5
28-29	3.095480206011351E-4	5.714732688020956E-4	0.0	5.833789619021393E-4	7.143415860026195E-5
30-31	3.095480206011351E-4	6.905301998025321E-4	0.0	0.0019942035942573126	7.143415860026195E-5
32-33	3.095480206011351E-4	6.905301998025321E-4	0.0	0.004839664245167748	7.143415860026195E-5
34-35	3.155008671511569E-4	8.33398517003056E-4	0.0	0.008012531456329382	8.33398517003056E-5
36-37	3.2145371370117875E-4	9.7626683420358E-4	0.0	0.013876085308100885	8.33398517003056E-5
38-39	3.2145371370117875E-4	9.7626683420358E-4	0.0	0.025115059594542098	8.33398517003056E-5
40-41	3.333594068012224E-4	0.001119135151404104	0.0	0.06474911192458743	9.524554480034927E-5
42-43	3.393122533512442E-4	0.0011369936910541694	0.0	0.07557733979907715	9.524554480034927E-5
44-45	3.4526509990126606E-4	0.0012679563151546495	0.0	0.0920071962771374	9.524554480034927E-5
46-47	3.4526509990126606E-4	0.0013810603996050642	0.0	0.10991931154615307	9.524554480034927E-5
48-49	3.4526509990126606E-4	0.0013810603996050642	0.0	0.12724804785326663	9.524554480034927E-5
50-51	3.5717079300130977E-4	0.001500117330605501	0.0	0.14405888651052828	1.0715123790039293E-4
52-53	3.5717079300130977E-4	0.0015120230237055446	0.0	0.17460294215869027	1.1310408445041475E-4
54-55	3.690764861013534E-4	0.0015179758702555664	0.0	0.25238283518127547	1.250097775504584E-4
56-57	3.809821792013971E-4	0.0015239287168055883	0.0	0.3394491688218948	1.428683172005239E-4
58-59	3.809821792013971E-4	0.00152988156335561	0.0	0.43350414431223966	1.428683172005239E-4
60-61	3.9884071885146254E-4	0.001565598642655741	0.0	0.45444625847521647	1.4882116375054572E-4
62-63	4.047935654014844E-4	0.001631079954705981	0.0	0.467691342049015	1.5477401030056755E-4
64-65	4.047935654014844E-4	0.001976345054607247	0.0	0.4782874089080539	1.5477401030056755E-4
66-67	4.1669925850152805E-4	0.0019942035942573126	0.0	0.48511532390092893	1.6072685685058937E-4
68-69	4.1669925850152805E-4	0.0020477792132075094	0.0	0.4918420405024536	1.666797034006112E-4
70-71	4.1669925850152805E-4	0.0020894491390576623	0.0	0.4978067927455755	1.666797034006112E-4
72-73	4.2265210505154985E-4	0.002119213371807771	0.0	0.5017356714685899	1.666797034006112E-4
74-75	4.286049516015717E-4	0.002172788990757968	0.0	0.505039501303852	1.666797034006112E-4
76-77	4.286049516015717E-4	0.002268034535558317	0.0	0.5078254334892622	1.7263254995063303E-4
78-79	4.286049516015717E-4	0.002333515847608557	0.0	0.5103434875799214	1.7858539650065489E-4
80-81	4.286049516015717E-4	0.002333515847608557	0.0	0.5115161983502757	1.9049108960069854E-4
82-83	4.286049516015717E-4	0.002333515847608557	0.0	0.5120519545397777	2.0834962925076402E-4
84-85	4.286049516015717E-4	0.002333515847608557	0.0	0.5124924651844793	2.262081689008295E-4
86-87	4.286049516015717E-4	0.002369232926908688	0.0	0.51268890912063	2.3811386200087316E-4
88-89	4.4051064470161536E-4	0.0024644784717090373	0.0	0.5127424847395802	2.3811386200087316E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13265	0.0	55.982414	1
GTATCAA	25510	0.0	40.394314	1
TCAACGC	30105	0.0	33.709026	4
ATCAACG	30215	0.0	33.602055	3
TATCAAC	30795	0.0	33.180344	2
CAACGCA	31045	0.0	32.688362	5
AACGCAG	31835	0.0	32.00173	6
GTGGTAT	5970	0.0	29.62698	1
TGGTATC	5650	0.0	29.233313	2
ACGCAGA	36055	0.0	28.280174	7
CGCAGAG	39315	0.0	25.891096	8
GCAGAGT	42590	0.0	23.900173	9
GAGTACT	25400	0.0	22.340736	12-13
GTACATG	30085	0.0	21.686426	1
TACATGG	30005	0.0	21.253016	2
CAGAGTA	41260	0.0	21.241491	10-11
ACATGGG	30035	0.0	20.640013	3
GTACTTT	28210	0.0	20.08507	14-15
CATGGGG	23310	0.0	19.247818	4
AGAGTAC	38810	0.0	18.8587	10-11
>>END_MODULE