FastQCFastQC Report
Fri 27 May 2016
SRR2140160_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140160_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11665716
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA201350.051.0989571
GTATCAA363200.037.2519571
ATCAACG416200.031.8238123
TCAACGC419450.031.5863044
TATCAAC431300.030.9798182
CAACGCA427350.030.9620085
AACGCAG438500.030.2627546
GTGGTAT97350.027.48611
TGGTATC93950.027.1486422
ACGCAGA492250.026.9144697
CGCAGAG501500.026.4438488
GCAGAGT573500.023.0562539
GAGTACT322200.022.24005112-13
GTACATG436300.020.9308991
CAGAGTA565850.020.59277710-11
TACATGG431100.020.432542
ACATGGG428150.019.9685233
GTACTTT355950.019.94214814-15
AGAGTAC535650.018.25434910-11
CATGGGG287300.017.8602244
Produced by FastQC (version 0.11.5)