##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140160_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11665716
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.640104902262323	30.0	18.0	33.0	18.0	33.0
2	30.041405345372713	32.0	28.0	33.0	25.0	33.0
3	30.737675081409492	33.0	30.0	33.0	27.0	33.0
4	31.394294957977717	33.0	31.0	33.0	28.0	33.0
5	31.663910556368766	33.0	32.0	33.0	30.0	34.0
6	34.56355435020019	37.0	34.0	38.0	28.0	38.0
7	35.70060311771691	38.0	36.0	38.0	31.0	38.0
8	35.58920498321749	38.0	36.0	38.0	31.0	38.0
9	36.26497713470823	38.0	37.0	38.0	33.0	38.0
10-11	36.55211343221453	38.0	37.5	38.0	34.0	38.0
12-13	36.66731660534167	38.0	38.0	38.0	34.0	38.0
14-15	36.67321337155816	38.0	38.0	38.0	34.0	38.0
16-17	36.671222366462544	38.0	38.0	38.0	34.0	38.0
18-19	36.700870653802994	38.0	38.0	38.0	34.0	38.0
20-21	36.72447952693351	38.0	38.0	38.0	34.0	38.0
22-23	36.74329501508281	38.0	38.0	38.0	34.0	38.0
24-25	36.74496106405871	38.0	38.0	38.0	34.0	38.0
26-27	36.69867696008467	38.0	38.0	38.0	34.0	38.0
28-29	36.68445837223936	38.0	38.0	38.0	34.0	38.0
30-31	36.67816928958611	38.0	38.0	38.0	34.0	38.0
32-33	36.662705737103785	38.0	38.0	38.0	34.0	38.0
34-35	36.63010490664111	38.0	38.0	38.0	34.0	38.0
36-37	36.60156765332839	38.0	38.0	38.0	34.0	38.0
38-39	36.58942219182126	38.0	38.0	38.0	34.0	38.0
40-41	36.57904628332744	38.0	38.0	38.0	34.0	38.0
42-43	36.54199115190282	38.0	38.0	38.0	34.0	38.0
44-45	36.47624269749157	38.0	38.0	38.0	34.0	38.0
46-47	36.42776271517698	38.0	38.0	38.0	34.0	38.0
48-49	36.39208850815619	38.0	38.0	38.0	34.0	38.0
50-51	36.376430537800786	38.0	38.0	38.0	34.0	38.0
52-53	36.34860770787529	38.0	38.0	38.0	33.5	38.0
54-55	36.31405102848278	38.0	38.0	38.0	33.0	38.0
56-57	36.278550607868226	38.0	37.5	38.0	33.0	38.0
58-59	36.25021133914518	38.0	37.0	38.0	33.0	38.0
60-61	36.24827572454473	38.0	37.0	38.0	33.0	38.0
62-63	36.23089345672245	38.0	37.0	38.0	33.0	38.0
64-65	36.21990489512939	38.0	37.0	38.0	33.0	38.0
66-67	36.19146936491845	38.0	37.0	38.0	33.0	38.0
68-69	36.18023653854315	38.0	37.0	38.0	33.0	38.0
70-71	36.165975740713094	38.0	37.0	38.0	33.0	38.0
72-73	36.144206427195925	38.0	37.0	38.0	33.0	38.0
74-75	36.13477852930145	38.0	37.0	38.0	33.0	38.0
76-77	36.113212883611666	38.0	37.0	38.0	33.0	38.0
78-79	36.12373087897721	38.0	37.0	38.0	33.0	38.0
80-81	36.075756543890975	38.0	37.0	38.0	33.0	38.0
82-83	36.07460599041569	38.0	37.0	38.0	33.0	38.0
84-85	36.06168057393033	38.0	37.0	38.0	33.0	38.0
86-87	36.01944010883669	38.0	37.0	38.0	31.5	38.0
88-89	36.008644380372296	38.0	37.0	38.0	31.5	38.0
90-91	36.00768611520806	38.0	37.0	38.0	31.5	38.0
92-93	35.98752956474972	38.0	37.0	38.0	31.0	38.0
94-95	35.96414984568146	38.0	37.0	38.0	31.0	38.0
96-97	35.97304567364165	38.0	37.0	38.0	31.5	38.0
98-99	36.002579369002405	38.0	37.0	38.0	32.0	38.0
100-101	35.38326080320282	38.0	35.5	38.0	30.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	4.0
17	12.0
18	21.0
19	54.0
20	164.0
21	888.0
22	3351.0
23	9527.0
24	21339.0
25	39038.0
26	62937.0
27	93272.0
28	130116.0
29	171615.0
30	220350.0
31	278903.0
32	353244.0
33	461999.0
34	638064.0
35	996653.0
36	2156371.0
37	6027794.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.91061397345864	18.198377193478738	14.43900228670062	20.452006546362007
2	15.129830007862354	20.210135408748165	43.43613371009546	21.223900873294017
3	19.31059023600199	26.563813325403252	28.908865734333233	25.216730704261526
4	12.742604054478953	18.772941155090695	38.29009723878071	30.194357551649638
5	14.285705710310156	37.48138494148744	34.71207823375488	13.520831114447516
6	30.036621841299755	37.625611664127604	18.6930146422217	13.64475185235094
7	27.143597529718704	33.00012618171058	22.15610254869911	17.7001737398716
8	24.2274970520455	37.40408218406826	20.37844912391147	17.989971639974776
9	25.465123615215727	18.052668177418344	21.26480706370702	35.217401143658904
10-11	24.50974072313613	27.18501180596303	27.97283749860167	20.33240997229917
12-13	25.35418677100672	24.574778072730723	28.49592641334716	21.575108742915397
14-15	22.35998297746932	25.981166778840098	26.62393061102853	25.03491963266205
16-17	21.548351597107285	29.208005749497072	27.235880763769664	22.007761889625975
18-19	21.46599917227541	28.10630311932847	29.398354117312646	21.029343591083478
20-21	22.53402652139264	27.040722076182522	29.46447951369316	20.96077188873168
22-23	22.171032154884944	26.97596237674424	29.30150158706171	21.551503881309106
24-25	21.849752809258653	27.14109302658748	29.41903098484257	21.590123179311295
26-27	21.762262792391027	27.298590444278915	29.3433942272011	21.59575253612896
28-29	21.627989654460137	27.409992927491423	29.352301415583455	21.60971600246499
30-31	21.994664607190202	27.204199248675554	29.298267228501977	21.50286891563227
32-33	21.722255694904582	27.322008921841718	29.243163395397904	21.712571987855796
34-35	21.88316589758428	27.39875959727014	29.256281549335306	21.461792955810274
36-37	21.87343281564344	27.31717969860664	29.16174620788507	21.647641277864846
38-39	21.69669446212423	27.470668561775184	29.196844265179518	21.635792710921066
40-41	22.003796880863554	27.337568530612195	29.045577261798	21.613057326726253
42-43	21.87299317949764	27.4003101741327	28.861517195699516	21.86517945067014
44-45	21.955599024128365	27.5251399335018	28.729358020425533	21.789903021944298
46-47	21.979633324059105	27.575796180974432	28.42267343640567	22.02189705856079
48-49	21.943143395360487	27.66207441266244	28.386976742669745	22.007805449307323
50-51	21.90230829777217	27.791958122151485	28.259893511630946	22.0458400684454
52-53	22.12712925283479	27.867282858903792	27.918403737651783	22.08718415060963
54-55	22.1115834940586	27.770095385190825	27.976431489214075	22.141889631536504
56-57	22.065970237531662	27.847552387064045	27.960271511513056	22.126205863891236
58-59	22.06337682581089	27.886845408722493	28.01282911022003	22.036948655246587
60-61	22.12309329246238	27.796113371306646	28.03448505788701	22.046308278343965
62-63	22.08779668559235	27.87567409831093	27.985639915657767	22.050889300438943
64-65	22.01281655685485	27.8858528299724	27.98827857022524	22.113052042947505
66-67	22.109672255252917	27.886975974569744	27.82307343023079	22.18027833994655
68-69	22.04174639626001	27.879456953185382	27.912056370979997	22.166740279574608
70-71	22.041271725646666	27.641617163385323	27.95672953442635	22.36038157654166
72-73	22.07614382350129	27.7175331281707	27.905495302020267	22.300827746307743
74-75	21.9417494032916	27.80325659340917	27.922605835303287	22.332388167995944
76-77	22.11544252258279	27.747854233121043	27.799130665839815	22.33757257845635
78-79	22.025190067550017	27.778644466383817	27.77300614063997	22.42315932542619
80-81	22.063629886542024	27.80184321686991	27.75565672957866	22.378870167009403
82-83	22.14084046871028	27.78189585273177	27.865729589804104	22.21153408875384
84-85	22.258418361585523	27.82493101997037	27.753384420718756	22.16326619772535
86-87	22.09960038678207	27.90141147474466	27.78777328470916	22.211214853764115
88-89	22.14941373628303	27.849974052492556	27.772668226339935	22.227943984884476
90-91	22.17224368080534	27.87816744936149	27.7836050027035	22.165983867129675
92-93	22.15717696127292	27.814358804020063	27.85157935547883	22.17688487922819
94-95	22.001799603596307	27.97298586368421	27.829893390501148	22.195321142218333
96-97	22.030078140116643	28.009623242214587	27.804514969440813	22.155783648227956
98-99	22.269462383645873	28.54015685926775	27.8533768601032	21.337003896983177
100-101	22.938350261488882	31.050406057920593	25.269913677947514	20.74133000264301
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4011.0
1	3392.5
2	3081.0
3	3934.5
4	5384.5
5	7739.0
6	9589.5
7	10066.5
8	9865.5
9	9496.0
10	9212.0
11	9927.0
12	12163.5
13	18689.5
14	30020.0
15	44000.0
16	57279.5
17	66187.0
18	70283.0
19	66070.0
20	58469.0
21	52126.5
22	48602.0
23	51425.0
24	59331.0
25	73044.0
26	91317.5
27	111821.5
28	135542.5
29	161743.5
30	188412.0
31	215931.0
32	244967.0
33	275046.0
34	305382.0
35	332132.0
36	360305.0
37	388742.5
38	408432.0
39	423445.5
40	439782.0
41	454599.5
42	463392.0
43	471178.0
44	478112.5
45	485183.0
46	489742.0
47	488600.0
48	487468.5
49	480678.0
50	468569.0
51	453450.0
52	429796.0
53	403725.5
54	379086.0
55	351927.0
56	322379.5
57	289984.0
58	257239.5
59	223429.0
60	187162.0
61	152607.5
62	119555.0
63	91852.5
64	71048.5
65	54356.5
66	40718.5
67	29898.5
68	21971.0
69	16381.5
70	12080.0
71	8405.0
72	5770.0
73	3869.5
74	2577.5
75	1625.5
76	1019.0
77	654.5
78	406.0
79	248.5
80	157.0
81	103.5
82	74.5
83	58.0
84	40.5
85	26.5
86	16.0
87	8.5
88	5.5
89	3.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.003711731024482338
4	0.0
5	0.038214542510721164
6	0.0
7	0.0
8	0.0
9	0.0
10-11	8.572127077326415E-6
12-13	1.157237155439066E-4
14-15	4.286063538663207E-6
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	1.7797156112337605E-5
46-47	0.0010144988950042124
48-49	0.0
50-51	8.672055451495961E-5
52-53	0.0012586421574767885
54-55	6.034268798174642E-4
56-57	0.0036374767263438828
58-59	0.008615500798005462
60-61	0.010893470689203393
62-63	0.005997688711914258
64-65	0.008176601865046705
66-67	0.005166031987369587
68-69	0.002571763868298009
70-71	0.004443098031088411
72-73	0.0028588866066110554
74-75	0.0012552307582954874
76-77	0.0020650532463298257
78-79	7.702571467539103E-4
80-81	3.423130518209576E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	1.9856804858077624E-4
94-95	3.1796986616313626E-4
96-97	1.4123559679383896E-4
98-99	4.893853768270314E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1185.0
20-21	4325.0
22-23	2550.0
24-25	3063.0
26-27	4708.0
28-29	9317.0
30-31	22135.0
32-33	27235.0
34-35	37735.0
36-37	60869.0
38-39	73688.0
40-41	72912.0
42-43	84396.0
44-45	98091.0
46-47	97143.0
48-49	90981.0
50-51	112479.0
52-53	72473.0
54-55	76374.0
56-57	78473.0
58-59	84562.0
60-61	85599.0
62-63	79831.0
64-65	84483.0
66-67	90672.0
68-69	92277.0
70-71	92821.0
72-73	85638.0
74-75	86851.0
76-77	91806.0
78-79	95919.0
80-81	98459.0
82-83	91227.0
84-85	89109.0
86-87	92341.0
88-89	98604.0
90-91	105651.0
92-93	100210.0
94-95	107071.0
96-97	169967.0
98-99	975609.0
100-101	7736877.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.26808224459108
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.3762400856912	71.00899465507088
2	8.368368280430067	13.60162483334036
3	2.28359507118014	5.5675017617403135
4	0.8931282041782458	2.9033126540848646
5	0.4097013571210227	1.6647821793115927
6	0.22430790233234324	1.0937443832913947
7	0.1401136942532717	0.797073985971786
8	0.07978960263411264	0.5187478391305846
9	0.05475503022128881	0.4004852669395892
>10	0.16606836329427235	2.0500198450494898
>50	0.0028931973427430415	0.1542955545777884
>100	9.254465130865948E-4	0.15174136126861604
>500	9.48051063012487E-5	0.05309971051473141
>1k	1.8959701913500985E-5	0.03457596970801854
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.572127077326415E-6
2	0.0	2.5716381231979246E-5	0.0	0.0	8.572127077326415E-6
3	0.0	3.428850830930566E-5	0.0	0.0	8.572127077326415E-6
4	0.0	6.857701661861132E-5	0.0	2.5716381231979246E-5	8.572127077326415E-6
5	0.0	6.857701661861132E-5	0.0	2.5716381231979246E-5	8.572127077326415E-6
6	1.628704144692019E-4	7.714914369593773E-5	0.0	2.5716381231979246E-5	8.572127077326415E-6
7	1.714425415465283E-4	7.714914369593773E-5	0.0	4.2860635386632075E-5	8.572127077326415E-6
8	1.714425415465283E-4	8.572127077326415E-5	0.0	4.2860635386632075E-5	8.572127077326415E-6
9	1.714425415465283E-4	1.628704144692019E-4	0.0	5.143276246395849E-5	2.5716381231979246E-5
10-11	1.8858679570118113E-4	1.628704144692019E-4	0.0	6.00048895412849E-5	2.5716381231979246E-5
12-13	1.9715892277850756E-4	1.628704144692019E-4	0.0	6.857701661861132E-5	3.000244477064245E-5
14-15	2.0573104985583397E-4	2.6573593939711887E-4	0.0	8.572127077326415E-5	5.57188260026217E-5
16-17	2.6573593939711887E-4	2.7002200293578207E-4	0.0	9.429339785059057E-5	7.714914369593773E-5
18-19	2.7430806647444527E-4	2.8716625709043493E-4	0.0	1.0286552492791698E-4	8.143520723460093E-5
20-21	2.7430806647444527E-4	3.0002444770642453E-4	0.0	1.157237155439066E-4	8.572127077326415E-5
22-23	3.2145476539974054E-4	3.214547653997406E-4	0.0	1.5858435093053867E-4	9.857946138925377E-5
24-25	3.9003178201835187E-4	3.7288752786369906E-4	0.0	2.5716381231979246E-4	1.3715403323722263E-4
26-27	3.943178455570151E-4	3.986039090956783E-4	0.0	3.6860146432503586E-4	1.4144009677588586E-4
28-29	3.943178455570151E-4	4.0717603617300473E-4	0.0	8.357823900393255E-4	1.671564780078651E-4
30-31	4.243202903276576E-4	4.371784809436472E-4	0.0	0.002023021990249034	1.8430073216251793E-4
32-33	4.414645444823104E-4	4.371784809436472E-4	0.0	0.004654665002988244	1.9715892277850756E-4
34-35	4.7146698925295286E-4	4.928973069462689E-4	0.0	0.008006366690222873	1.9715892277850756E-4
36-37	5.057554975622585E-4	5.486161329488905E-4	0.0	0.01349681408325044	2.0144498631717076E-4
38-39	5.057554975622585E-4	5.786185777195331E-4	0.0	0.023881946037431392	2.185892404718236E-4
40-41	5.100415611009217E-4	6.514816578768076E-4	0.0	0.051878513071979465	2.228753040104868E-4
42-43	5.400440058715641E-4	6.771980391087868E-4	0.0	0.062019339404456614	2.2716136754915E-4
44-45	5.400440058715641E-4	7.500611192660613E-4	0.0	0.07550758136062974	2.314474310878132E-4
46-47	5.400440058715641E-4	7.972078181913567E-4	0.0	0.09048737342825763	2.357334946264764E-4
48-49	5.657603871035435E-4	7.972078181913567E-4	0.0	0.10389846624073482	2.5716381231979246E-4
50-51	5.914767683355227E-4	8.786430254259576E-4	0.0	0.11837678887433914	2.5716381231979246E-4
52-53	6.129070860288387E-4	8.829290889646208E-4	0.0	0.14072432416492908	2.6573593939711887E-4
54-55	6.343374037221547E-4	8.829290889646208E-4	0.0	0.18961545095046028	2.7430806647444527E-4
56-57	6.771980391087868E-4	8.915012160419472E-4	0.0	0.24187970974091944	2.7430806647444527E-4
58-59	6.943422932634396E-4	8.915012160419472E-4	0.0	0.3024932203046945	2.785941300131085E-4
60-61	6.986283568021028E-4	9.215036608125896E-4	0.0	0.322607716491641	2.828801935517717E-4
62-63	7.157726109567556E-4	9.343618514285793E-4	0.0	0.3330271369541312	3.0002444770642453E-4
64-65	7.329168651114085E-4	0.0010886601388204547	0.0	0.34278221756812866	3.0002444770642453E-4
66-67	7.543471828047245E-4	0.001097232265897781	0.0	0.348619836107788	3.0002444770642453E-4
68-69	7.543471828047245E-4	0.0011272347106684236	0.0	0.3542860121059007	3.0002444770642453E-4
70-71	7.586332463433877E-4	0.0011443789648230766	0.0	0.3596093030209204	3.1288263832241414E-4
72-73	7.629193098820509E-4	0.0011486650283617397	0.0	0.3638096452888104	3.343129560157302E-4
74-75	7.757775004980406E-4	0.001174381409593719	0.0	0.36697276018034386	3.343129560157302E-4
76-77	7.886356911140302E-4	0.0012043838543643612	0.0	0.36977584573462957	3.385990195543934E-4
78-79	8.014938817300199E-4	0.0012258141720576773	0.0	0.37272894351276853	3.428850830930566E-4
80-81	8.057799452686831E-4	0.0012258141720576773	0.0	0.3741304862899114	3.471711466317198E-4
82-83	8.057799452686831E-4	0.0012258141720576773	0.0	0.37467481635932165	3.7288752786369906E-4
84-85	8.143520723460095E-4	0.0012301002355963406	0.0	0.3751762857933452	3.7717359140236226E-4
86-87	8.229241994233359E-4	0.0012686748074443096	0.0	0.3753563004619691	3.7717359140236226E-4
88-89	8.272102629619991E-4	0.0013372518240629208	0.0	0.37540773322443305	3.8145965494102547E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	20135	0.0	51.098957	1
GTATCAA	36320	0.0	37.251957	1
ATCAACG	41620	0.0	31.823812	3
TCAACGC	41945	0.0	31.586304	4
TATCAAC	43130	0.0	30.979818	2
CAACGCA	42735	0.0	30.962008	5
AACGCAG	43850	0.0	30.262754	6
GTGGTAT	9735	0.0	27.4861	1
TGGTATC	9395	0.0	27.148642	2
ACGCAGA	49225	0.0	26.914469	7
CGCAGAG	50150	0.0	26.443848	8
GCAGAGT	57350	0.0	23.056253	9
GAGTACT	32220	0.0	22.240051	12-13
GTACATG	43630	0.0	20.930899	1
CAGAGTA	56585	0.0	20.592777	10-11
TACATGG	43110	0.0	20.43254	2
ACATGGG	42815	0.0	19.968523	3
GTACTTT	35595	0.0	19.942148	14-15
AGAGTAC	53565	0.0	18.254349	10-11
CATGGGG	28730	0.0	17.860224	4
>>END_MODULE