FastQCFastQC Report
Fri 27 May 2016
SRR2140159_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140159_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10166136
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA168900.056.018661
GTATCAA317650.039.4099161
TCAACGC378550.032.4617084
ATCAACG379800.032.344413
CAACGCA386950.031.8018465
TATCAAC388100.031.7979322
AACGCAG398300.030.9705186
GTGGTAT74650.028.8110771
TGGTATC69850.028.3698752
ACGCAGA445800.027.6318537
CGCAGAG455150.027.0451748
GCAGAGT514950.023.904489
GAGTACT320650.022.71607412-13
CAGAGTA510400.021.23450910-11
GTACATG344200.020.4463271
GTACTTT356000.020.38756814-15
TACATGG334550.020.1806532
AGAGTAC479300.019.22116510-11
ACATGGG345000.019.1671853
ACTTTTT402100.018.0727116-17
Produced by FastQC (version 0.11.5)