FastQCFastQC Report
Fri 27 May 2016
SRR2140158_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140158_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9665229
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA191300.046.2005231
CTTATAC83100.036.7260361
GTATCAA332100.035.9320031
ATCAACG375850.030.9499743
TCAACGC377350.030.8256664
CAACGCA382850.030.4164625
TATCAAC392650.029.9646342
AACGCAG396050.029.4440986
TATACAC124250.029.226683
ACGCAGA443800.026.256777
CGCAGAG456950.025.5011588
TTATACA142250.024.7136652
GCAGAGT507950.022.8816229
ACATCTC149650.022.6490088
ACACATC150500.022.4925796
TACACAT166600.022.3631135
TACATGG383900.022.284082
GTGGTAT100250.022.265091
GTACATG400100.021.9504091
GAGTACT275650.021.89953412-13
Produced by FastQC (version 0.11.5)