##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140158_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9665229
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.48555766242062	30.0	18.0	33.0	18.0	33.0
2	29.948783934658973	32.0	27.0	33.0	25.0	33.0
3	30.68017571027029	33.0	29.0	33.0	27.0	33.0
4	31.342939520625947	33.0	31.0	33.0	28.0	33.0
5	31.62510769274065	33.0	32.0	33.0	30.0	34.0
6	34.49955805496176	37.0	34.0	38.0	28.0	38.0
7	35.678758361545285	38.0	36.0	38.0	31.0	38.0
8	35.5753192190273	38.0	36.0	38.0	31.0	38.0
9	36.27689545690019	38.0	37.0	38.0	33.0	38.0
10-11	36.55937453732343	38.0	37.5	38.0	34.0	38.0
12-13	36.6681570090062	38.0	38.0	38.0	34.0	38.0
14-15	36.67228815789052	38.0	38.0	38.0	34.0	38.0
16-17	36.67153830498998	38.0	38.0	38.0	34.0	38.0
18-19	36.70019008344241	38.0	38.0	38.0	34.0	38.0
20-21	36.725919398415	38.0	38.0	38.0	34.0	38.0
22-23	36.743881730255495	38.0	38.0	38.0	34.0	38.0
24-25	36.73945908672237	38.0	38.0	38.0	34.0	38.0
26-27	36.69314491833248	38.0	38.0	38.0	34.0	38.0
28-29	36.68062690488564	38.0	38.0	38.0	34.0	38.0
30-31	36.66849708463838	38.0	38.0	38.0	34.0	38.0
32-33	36.650569346127135	38.0	38.0	38.0	34.0	38.0
34-35	36.6172442948228	38.0	38.0	38.0	34.0	38.0
36-37	36.58748780445907	38.0	38.0	38.0	34.0	38.0
38-39	36.55423552145407	38.0	38.0	38.0	34.0	38.0
40-41	36.57573996695551	38.0	38.0	38.0	34.0	38.0
42-43	36.5476124529599	38.0	38.0	38.0	34.0	38.0
44-45	36.47630954663519	38.0	38.0	38.0	34.0	38.0
46-47	36.42413548199957	38.0	38.0	38.0	34.0	38.0
48-49	36.38710515799512	38.0	38.0	38.0	34.0	38.0
50-51	36.37042311766976	38.0	38.0	38.0	34.0	38.0
52-53	36.34337239330583	38.0	38.0	38.0	33.5	38.0
54-55	36.31147683619463	38.0	38.0	38.0	33.0	38.0
56-57	36.27981968678886	38.0	37.5	38.0	33.0	38.0
58-59	36.25065839301314	38.0	37.0	38.0	33.0	38.0
60-61	36.248124248511886	38.0	37.0	38.0	33.0	38.0
62-63	36.23163331718651	38.0	37.0	38.0	33.0	38.0
64-65	36.225778406777394	38.0	37.0	38.0	33.0	38.0
66-67	36.197032273849956	38.0	37.0	38.0	33.0	38.0
68-69	36.18367896075456	38.0	37.0	38.0	33.0	38.0
70-71	36.16974664507865	38.0	37.0	38.0	33.0	38.0
72-73	36.14703833248726	38.0	37.0	38.0	33.0	38.0
74-75	36.141200286258865	38.0	37.0	38.0	33.0	38.0
76-77	36.12407297448505	38.0	37.0	38.0	33.0	38.0
78-79	36.139306060223205	38.0	37.0	38.0	33.0	38.0
80-81	36.092573245683454	38.0	37.0	38.0	33.0	38.0
82-83	36.09434972176603	38.0	37.0	38.0	33.0	38.0
84-85	36.08370740281927	38.0	37.0	38.0	33.0	38.0
86-87	36.0466186779427	38.0	37.0	38.0	33.0	38.0
88-89	36.03646002205398	38.0	37.0	38.0	32.5	38.0
90-91	36.03906282062738	38.0	37.0	38.0	32.5	38.0
92-93	36.01953184140966	38.0	37.0	38.0	32.5	38.0
94-95	35.99883192453747	38.0	37.0	38.0	32.0	38.0
96-97	36.00789803764376	38.0	37.0	38.0	32.5	38.0
98-99	36.05004830059751	38.0	37.0	38.0	33.0	38.0
100-101	35.449715422882576	38.0	35.5	38.0	30.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	3.0
17	11.0
18	20.0
19	46.0
20	170.0
21	708.0
22	2770.0
23	7976.0
24	17241.0
25	32162.0
26	51767.0
27	77370.0
28	108225.0
29	143810.0
30	184893.0
31	232092.0
32	294008.0
33	383077.0
34	530652.0
35	828527.0
36	1809573.0
37	4960128.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.06141985875348	17.942254653252395	14.232844353713709	20.76348113428042
2	15.070299938056305	20.090812126644906	43.47847319499621	21.360414740302584
3	19.11513614173582	26.793379393004795	29.149172869036615	24.94231159622277
4	12.850124916854014	18.822895970700745	37.761019423337	30.565959689108247
5	14.302286659301364	37.57377463872654	34.550212502838576	13.57372619913351
6	30.107967436674286	37.34871672466322	18.50537633407341	14.037939504589078
7	27.006964863429516	33.08093372645387	21.860878826564793	18.051222583551823
8	24.5433398422324	37.030503881491065	20.379299859320458	18.046856416956082
9	25.82954837386677	17.639468242294104	20.79632050104555	35.734662882793565
10-11	24.728339324828173	26.988420397924372	27.755572462668628	20.52766781457883
12-13	25.555719373175158	24.196924643205225	28.25551306643822	21.991842917181398
14-15	22.625045924933595	25.578969727463257	26.298895763359564	25.49708858424358
16-17	21.911193206079236	28.925636423099753	26.77882231243564	22.384348058385374
18-19	21.899248326138988	27.768581582495354	28.87067652509837	21.461493566267286
20-21	22.957532370708623	26.676308851270882	28.859983723056835	21.506175054963663
22-23	22.62096441299835	26.70922914196731	28.74349599776603	21.926310447268314
24-25	22.36606747634414	26.886672937965393	28.875313155075894	21.87194643061457
26-27	22.25789477472771	27.04061359460169	28.869128241957405	21.832363388713194
28-29	22.070429128523017	27.138392763464168	28.808192785691695	21.982985322321124
30-31	22.542433850083967	26.832073551055867	28.834010879643134	21.79148171921703
32-33	22.022869939983288	26.862566265302597	28.974001489983365	22.140562304730757
34-35	22.031461530014894	27.060718125289014	28.866243287649475	22.04157705704662
36-37	22.153038264666986	27.039345203470084	29.016736387445413	21.790880144417514
38-39	22.056347135090935	27.122627495904332	28.872418916741953	21.94860645226278
40-41	22.396812127959258	26.94529484303941	28.745945401275673	21.911947627725652
42-43	22.344672539183044	26.964192349258827	28.576998281489203	22.11413683006893
44-45	22.246267732795005	27.298079219695463	28.43315442735186	22.02249862015767
46-47	22.433078618685865	26.979950151341974	28.080365596604057	22.506605633368103
48-49	22.366654845489037	27.12920868168735	28.065837739644916	22.438298733178694
50-51	22.113984690339507	27.276222111491315	28.224240128450795	22.385553069718384
52-53	22.44378328940961	27.292509936136838	27.975753635642043	22.28795313881151
54-55	22.31630054467005	27.174776451140982	27.910200329985656	22.598722674203316
56-57	22.576618822701153	27.500891344283	27.498486129551093	22.424003703464752
58-59	22.26736060728496	27.816975967342877	27.892185898682794	22.02347752668937
60-61	22.560867642459446	27.945336729646936	27.46180801620931	22.031987611684308
62-63	22.26954813259206	28.164784536191412	27.44724592660257	22.11842140461396
64-65	22.174583607524397	28.108639059418095	27.540081149826385	22.176696183231122
66-67	22.387465905921616	28.05546167045621	27.278506667549056	22.278565756073117
68-69	22.30139706803584	27.898023695682266	27.518578426273137	22.282000810008757
70-71	22.316260391570104	27.446044543162373	27.630264712815283	22.60743035245224
72-73	22.445732615341512	27.47039297435941	27.595029065673877	22.488845344625204
74-75	22.20465237765809	27.565217498439903	27.680242518476263	22.54988760542574
76-77	22.344919014294	27.50368837449104	27.555867426538228	22.595525184676728
78-79	22.356756065902953	27.588809421831595	27.453060551654534	22.601373960610918
80-81	22.41132480292551	27.536495129384686	27.410901464181258	22.641278603508542
82-83	22.48643574974522	27.486189686248537	27.60547281100888	22.42190175299736
84-85	22.682507978666784	27.493313553370534	27.424509462681655	22.39966900528102
86-87	22.299615524658932	27.62175248507933	27.473539057743967	22.605092932517767
88-89	22.378238235572017	27.57376296032088	27.50314638284987	22.544852421257232
90-91	22.554417733737676	27.534080961299672	27.48585386301194	22.425647441950712
92-93	22.620528604325845	27.527188698873022	27.377883764013887	22.474398932787242
94-95	22.38473494260593	27.762908581177896	27.340739899759203	22.511616576456966
96-97	22.310327850803546	27.785499282283233	27.472875291778003	22.43129757513522
98-99	22.551165332054502	28.324779494847874	27.450667046525055	21.67338812657257
100-101	23.258272769197948	30.849958928382087	24.832895044450602	21.058873257969363
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2408.0
1	2049.0
2	1975.0
3	2757.0
4	3956.0
5	5844.0
6	7416.0
7	7940.0
8	7894.0
9	7744.5
10	7693.5
11	8414.0
12	10444.5
13	15991.0
14	25272.0
15	36793.0
16	47556.0
17	54022.5
18	56404.0
19	52734.5
20	46934.5
21	41832.0
22	38872.5
23	41489.5
24	48486.5
25	60373.0
26	77514.5
27	95789.5
28	113116.0
29	131332.5
30	147901.5
31	164581.5
32	184621.0
33	207561.0
34	231375.5
35	252116.0
36	274741.0
37	295975.5
38	310383.0
39	322824.0
40	339862.0
41	354343.5
42	362540.5
43	371538.0
44	383855.0
45	409185.0
46	425304.5
47	422972.0
48	420198.0
49	413117.0
50	403924.0
51	393857.5
52	376195.5
53	358064.5
54	342595.0
55	320670.0
56	295165.5
57	266520.0
58	235685.5
59	205764.5
60	174115.0
61	141767.5
62	110603.0
63	84843.5
64	65009.5
65	49080.5
66	36720.5
67	26929.5
68	19330.5
69	14037.5
70	10012.5
71	6697.5
72	4375.0
73	2783.5
74	1789.5
75	1153.0
76	713.5
77	414.5
78	234.0
79	148.5
80	100.0
81	64.5
82	45.0
83	35.0
84	29.5
85	19.0
86	10.5
87	9.0
88	8.0
89	7.5
90	5.5
91	2.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0038798873777331094
4	0.0
5	0.038395365490046844
6	0.0
7	0.0
8	0.0
9	0.0
10-11	5.1731831703108116E-6
12-13	9.311729706559463E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	2.6879165464272002E-5
46-47	0.0010251909485945391
48-49	2.1887687163664907E-5
50-51	1.0480994922233777E-4
52-53	0.0013655435136292946
54-55	7.243124281793694E-4
56-57	0.003644474908129805
58-59	0.008484005737858031
60-61	0.010615949471532857
62-63	0.006144730867170646
64-65	0.007794270123070472
66-67	0.0050577218413699904
68-69	0.002693188663120854
70-71	0.004107708465298502
72-73	0.0027662218301067157
74-75	0.0013614065633718432
76-77	0.001970741513564297
78-79	6.716777593934762E-4
80-81	4.6931715432207163E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	2.3645522007664256E-4
94-95	2.0505528256242004E-4
96-97	1.5233714259282804E-4
98-99	6.000735947401549E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1088.0
20-21	3725.0
22-23	2134.0
24-25	2424.0
26-27	4039.0
28-29	7665.0
30-31	19321.0
32-33	23563.0
34-35	31897.0
36-37	52283.0
38-39	64154.0
40-41	61975.0
42-43	69973.0
44-45	82025.0
46-47	81927.0
48-49	75757.0
50-51	94467.0
52-53	64859.0
54-55	68883.0
56-57	71335.0
58-59	73188.0
60-61	73318.0
62-63	71585.0
64-65	81566.0
66-67	88053.0
68-69	87154.0
70-71	100421.0
72-73	72221.0
74-75	72716.0
76-77	76425.0
78-79	80334.0
80-81	82459.0
82-83	76769.0
84-85	75086.0
86-87	77584.0
88-89	82665.0
90-91	88436.0
92-93	84657.0
94-95	89378.0
96-97	140758.0
98-99	803383.0
100-101	6303579.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.31241898460388
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.95578787137309	71.5189787551803
2	8.03150478142436	13.06122163728054
3	2.1235892031960373	5.180225251243719
4	0.8157789396258313	2.653318357506859
5	0.3878888847351982	1.577009175752958
6	0.21028904618711464	1.025946661886363
7	0.13000749530284217	0.7399856750442538
8	0.07653803840733903	0.4978794437789804
9	0.06170619097232111	0.4515731688856821
>10	0.1987847135342898	2.5226634935928245
>50	0.0058261422174625795	0.32579343929303284
>100	0.0021768144988168693	0.33706660503311464
>500	7.312743807085686E-5	0.042019463983407127
>1k	4.875108722905606E-5	0.06631887153797257
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	3.103909902186487E-5	0.0	1.0346366340621625E-5	0.0
5	0.0	3.103909902186487E-5	0.0	1.0346366340621625E-5	0.0
6	0.0	3.103909902186487E-5	0.0	5.173183170310812E-5	0.0
7	0.0	3.103909902186487E-5	0.0	5.173183170310812E-5	1.0346366340621625E-5
8	0.0	4.13854653624865E-5	0.0	5.173183170310812E-5	1.0346366340621625E-5
9	0.0	1.4484912876870274E-4	0.0	5.173183170310812E-5	1.0346366340621625E-5
10-11	0.0	1.4484912876870274E-4	0.0	5.173183170310812E-5	1.0346366340621625E-5
12-13	0.0	1.4484912876870274E-4	0.0	6.207819804372974E-5	1.0346366340621625E-5
14-15	0.0	1.8623459413118923E-4	0.0	6.207819804372974E-5	1.0346366340621625E-5
16-17	0.0	1.8623459413118923E-4	0.0	8.2770930724973E-5	1.0346366340621625E-5
18-19	0.0	2.0692732681243248E-4	0.0	8.2770930724973E-5	2.069273268124325E-5
20-21	0.0	2.2762005949367574E-4	0.0	9.829048023590543E-5	2.069273268124325E-5
22-23	0.0	2.7417870802647304E-4	0.0	1.0346366340621624E-4	2.069273268124325E-5
24-25	0.0	3.3625690607020275E-4	0.0	1.1898321291714867E-4	2.069273268124325E-5
26-27	0.0	3.621228219217568E-4	0.0	1.6036867827963518E-4	3.103909902186487E-5
28-29	0.0	3.621228219217568E-4	0.0	3.6729600509206764E-4	3.6212282192175685E-5
30-31	0.0	4.242010199654866E-4	0.0	0.0010087707182106084	4.13854653624865E-5
32-33	0.0	4.242010199654866E-4	0.0	0.002364144708832041	4.13854653624865E-5
34-35	0.0	5.173183170310812E-4	0.0	0.004381686145253257	4.13854653624865E-5
36-37	0.0	6.104356140966758E-4	0.0	0.007128646408688299	4.13854653624865E-5
38-39	0.0	6.311283467779191E-4	0.0	0.011251673395426017	4.13854653624865E-5
40-41	0.0	7.13899277502892E-4	0.0	0.016264487887457192	4.13854653624865E-5
42-43	0.0	7.294188270138245E-4	0.0	0.02069790586441356	4.13854653624865E-5
44-45	0.0	7.96670208227865E-4	0.0	0.02598489906447121	4.13854653624865E-5
46-47	0.0	8.380556735903516E-4	0.0	0.031752998299367764	4.13854653624865E-5
48-49	0.0	8.380556735903516E-4	0.0	0.03732968975696282	4.13854653624865E-5
50-51	0.0	9.156534211450138E-4	0.0	0.0429632862294313	4.13854653624865E-5
52-53	0.0	9.208266043153246E-4	0.0	0.04830201126119205	4.13854653624865E-5
54-55	0.0	9.208266043153246E-4	0.0	0.05432876965460415	4.13854653624865E-5
56-57	0.0	9.208266043153246E-4	0.0	0.06005031024096791	4.13854653624865E-5
58-59	0.0	9.259997874856353E-4	0.0	0.06532695707468494	4.13854653624865E-5
60-61	0.0	9.466925201668786E-4	0.0	0.07086743625008782	4.13854653624865E-5
62-63	0.0	9.829048023590544E-4	0.0	0.07609752443527204	4.13854653624865E-5
64-65	0.0	0.0011484466638090002	0.0	0.08126553442241255	4.13854653624865E-5
66-67	0.0	0.0011484466638090002	0.0	0.08661460582051392	4.13854653624865E-5
68-69	0.0	0.0011898321291714867	0.0	0.09193263811959343	4.13854653624865E-5
70-71	0.0	0.00121052486185273	0.0	0.09694027942845432	4.13854653624865E-5
72-73	0.0	0.00121052486185273	0.0	0.10115642371225761	4.13854653624865E-5
74-75	0.0	0.0012363907777042842	0.0	0.10450864640661903	4.13854653624865E-5
76-77	0.0	0.001324334891599568	0.0	0.10728147258590562	5.173183170310812E-5
78-79	0.0	0.0013450276242808112	0.0	0.10977494687399544	6.207819804372974E-5
80-81	0.0	0.0013450276242808112	0.0	0.11102685720121065	6.207819804372974E-5
82-83	0.0	0.0013450276242808112	0.0	0.11147692413702769	6.207819804372974E-5
84-85	0.0	0.0013553739906214328	0.0	0.11187008605797132	6.207819804372974E-5
86-87	0.0	0.0013657203569620545	0.0	0.11209253293429466	6.207819804372974E-5
88-89	0.0	0.0013967594559839193	0.0	0.11214426476599779	6.207819804372974E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	19130	0.0	46.200523	1
CTTATAC	8310	0.0	36.726036	1
GTATCAA	33210	0.0	35.932003	1
ATCAACG	37585	0.0	30.949974	3
TCAACGC	37735	0.0	30.825666	4
CAACGCA	38285	0.0	30.416462	5
TATCAAC	39265	0.0	29.964634	2
AACGCAG	39605	0.0	29.444098	6
TATACAC	12425	0.0	29.22668	3
ACGCAGA	44380	0.0	26.25677	7
CGCAGAG	45695	0.0	25.501158	8
TTATACA	14225	0.0	24.713665	2
GCAGAGT	50795	0.0	22.881622	9
ACATCTC	14965	0.0	22.649008	8
ACACATC	15050	0.0	22.492579	6
TACACAT	16660	0.0	22.363113	5
TACATGG	38390	0.0	22.28408	2
GTGGTAT	10025	0.0	22.26509	1
GTACATG	40010	0.0	21.950409	1
GAGTACT	27565	0.0	21.899534	12-13
>>END_MODULE