FastQCFastQC Report
Fri 27 May 2016
SRR2140157_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140157_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6852715
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76400.047.4764631
CTTATAC105100.045.8111841
TATACAC137500.038.6415523
GTATCAA143300.037.83281
TTATACA138500.035.602382
TCAACGC167800.031.4332144
ATCAACG168250.031.4002863
CAACGCA172050.030.8067825
TATCAAC175600.030.0407032
AACGCAG180200.029.4850886
TACACAT189150.027.9534785
ACGCAGA205400.025.7629137
ACACATC197750.025.7370386
CGTATAC6900.025.566951
CGCAGAG208400.025.3714038
ATACACA219200.025.2597064
ACATCTC210350.024.2158378
CACATCT219200.023.1792647
GTGGTAT39300.021.4589141
TGGTATC38650.021.263172
Produced by FastQC (version 0.11.5)