##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140157_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6852715
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.763950054832282	30.0	18.0	32.0	18.0	33.0
2	26.018791967855076	28.0	18.0	32.0	18.0	33.0
3	30.944554822431694	31.0	30.0	33.0	27.0	33.0
4	30.710092861004725	32.0	31.0	33.0	27.0	33.0
5	32.494893337896	33.0	33.0	33.0	32.0	33.0
6	36.49995571098462	38.0	37.0	38.0	34.0	38.0
7	36.63062844434651	38.0	37.0	38.0	34.0	38.0
8	37.078149755243	38.0	38.0	38.0	36.0	38.0
9	37.07205640392166	38.0	38.0	38.0	36.0	38.0
10-11	37.18503345024563	38.0	38.0	38.0	36.0	38.0
12-13	37.306745282709116	38.0	38.0	38.0	36.5	38.0
14-15	37.31769554111035	38.0	38.0	38.0	36.5	38.0
16-17	37.41334237597799	38.0	38.0	38.0	37.0	38.0
18-19	37.40130933505917	38.0	38.0	38.0	37.0	38.0
20-21	37.42812560453652	38.0	38.0	38.0	37.0	38.0
22-23	37.43434072399987	38.0	38.0	38.0	37.0	38.0
24-25	37.45391703503145	38.0	38.0	38.0	37.0	38.0
26-27	37.4015183758502	38.0	38.0	38.0	37.0	38.0
28-29	37.36940577817424	38.0	38.0	38.0	37.0	38.0
30-31	37.38487656047518	38.0	38.0	38.0	37.0	38.0
32-33	37.36532293356866	38.0	38.0	38.0	37.0	38.0
34-35	37.33157068413064	38.0	38.0	38.0	37.0	38.0
36-37	37.30259779086334	38.0	38.0	38.0	37.0	38.0
38-39	37.29924648984575	38.0	38.0	38.0	37.0	38.0
40-41	37.3447721411389	38.0	38.0	38.0	37.0	38.0
42-43	37.32201634829154	38.0	38.0	38.0	37.0	38.0
44-45	37.29895625811463	38.0	38.0	38.0	37.0	38.0
46-47	37.29043093545063	38.0	38.0	38.0	37.0	38.0
48-49	37.23938767813077	38.0	38.0	38.0	37.0	38.0
50-51	37.17274064550239	38.0	38.0	38.0	37.0	38.0
52-53	37.17104130463815	38.0	38.0	38.0	36.5	38.0
54-55	37.11771332216489	38.0	38.0	38.0	36.5	38.0
56-57	37.102408313332575	38.0	38.0	38.0	36.0	38.0
58-59	37.107846666476576	38.0	38.0	38.0	36.0	38.0
60-61	37.115957885259874	38.0	38.0	38.0	36.0	38.0
62-63	37.126443038737946	38.0	38.0	38.0	36.0	38.0
64-65	37.13526741930118	38.0	38.0	38.0	36.0	38.0
66-67	37.13112247356507	38.0	38.0	38.0	36.0	38.0
68-69	37.12131214087189	38.0	38.0	38.0	36.0	38.0
70-71	37.117839248924156	38.0	38.0	38.0	36.0	38.0
72-73	37.11954832967679	38.0	38.0	38.0	36.0	38.0
74-75	37.11709755921633	38.0	38.0	38.0	36.0	38.0
76-77	37.1181234203164	38.0	38.0	38.0	36.0	38.0
78-79	37.106009086749964	38.0	38.0	38.0	36.0	38.0
80-81	37.107490554780824	38.0	38.0	38.0	36.0	38.0
82-83	37.06544790913299	38.0	38.0	38.0	36.0	38.0
84-85	37.054620515094086	38.0	38.0	38.0	36.0	38.0
86-87	37.05602566374049	38.0	38.0	38.0	36.0	38.0
88-89	37.039571388117665	38.0	38.0	38.0	36.0	38.0
90-91	37.04346846265797	38.0	38.0	38.0	36.0	38.0
92-93	37.047770458402766	38.0	38.0	38.0	36.0	38.0
94-95	37.057319728538594	38.0	38.0	38.0	36.0	38.0
96-97	37.07126694644165	38.0	38.0	38.0	36.0	38.0
98-99	37.06293947222444	38.0	38.0	38.0	36.0	38.0
100	35.644973424203314	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	5.0
18	17.0
19	31.0
20	51.0
21	146.0
22	387.0
23	1110.0
24	2689.0
25	5607.0
26	10251.0
27	16908.0
28	26381.0
29	38743.0
30	52848.0
31	69531.0
32	91480.0
33	127681.0
34	203400.0
35	402230.0
36	1236696.0
37	4566523.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.687625269692376	21.232022636283578	13.691974640708098	24.38837745331595
2	15.36835293604274	20.63470542812939	41.38787211787924	22.609069517948623
3	18.061177212243614	26.38577556486735	30.662095242542552	24.890951980346475
4	12.53119384068942	18.272524101761125	38.023469529960025	31.17281252758943
5	13.276154049891176	37.65886951376206	35.827434819629886	13.237541616716877
6	29.81745191504389	37.71214766701957	18.514048811310555	13.956351606625987
7	26.235265876371628	32.642376050952066	22.51116528266534	18.611192790010968
8	24.189157144285147	37.00204955262257	21.34943595348705	17.459357349605227
9	25.10539253419995	16.96752309121275	20.627328000653755	37.29975637393355
10-11	24.20580747922539	26.52435567508644	28.85810952301387	20.4117273226743
12-13	25.20964318521929	23.84901458764884	28.723951017954196	22.217391209177677
14-15	22.25210737641942	25.451656752104824	25.9160274431375	26.380208428338257
16-17	22.12149491114106	28.86245816439178	26.57549598954575	22.440550934921415
18-19	22.27298961068715	27.603614042025683	28.255676764610815	21.867719582676358
20-21	22.810378142241092	26.843871545433707	28.21922609893161	22.126524213393598
22-23	22.450549877929355	27.32637084214991	28.137752631948775	22.08532664797196
24-25	22.699429659417813	27.30160289207823	28.355425678658154	21.643541769845804
26-27	22.801239555246575	27.470590046921934	28.30708987242611	21.42108052540538
28-29	22.635287067786336	27.328946999545607	28.223409244621166	21.81235668804689
30-31	22.463199763525573	27.471773423007516	28.594646952669674	21.47037986079724
32-33	22.490812030671908	27.556513387660925	28.394606718012987	21.55806786365418
34-35	22.799166796081106	27.06185824260378	28.638933421481937	21.500041539833177
36-37	22.089631016634385	27.507682580704845	28.459952840201584	21.942733562459185
38-39	22.075866742249666	26.90118273275475	28.78017585410478	22.242774670890807
40-41	22.280972148366057	26.734906411925998	28.869587149690073	22.114534290017875
42-43	22.514727526720193	26.628202204985733	28.788656155199664	22.06841411309441
44-45	22.07620337916623	27.05011434996801	28.67939987473409	22.194282396131673
46-47	22.634980261764497	26.624250638748435	28.103322554283466	22.6374465452036
48-49	22.382473928618165	26.69047396286227	28.201915468428773	22.725136640090795
50-51	22.067689833905	26.987994306264078	28.53475716745718	22.40955869237374
52-53	22.29407346947876	27.09984323903806	28.72964939008458	21.876433901398602
54-55	22.21446308213087	27.172724948903127	28.189490766580022	22.423321202385978
56-57	22.57021514050321	27.806239374931728	27.569152192578088	22.054393291986973
58-59	21.87903539236221	28.45394849808109	28.13318945096677	21.533826658589927
60-61	22.316127960799363	29.026158853815748	27.18845407797118	21.469259107413713
62-63	21.848493812433816	29.39660307145703	27.18334066846718	21.571562447641977
64-65	21.844128318061937	29.202592682427948	27.24594095703695	21.707338042473165
66-67	22.017475502875097	29.054844763444443	27.17986944514555	21.74781028853491
68-69	22.06153031632762	28.586278594885904	27.42366630755255	21.928524781233925
70-71	22.232566643936895	27.942293224096236	27.636280067669293	22.188860064297568
72-73	22.46718724012284	27.56514169119255	27.686515485405046	22.281155583279567
74-75	22.27841019370033	27.654678045123816	27.737003944236143	22.32990781693971
76-77	22.494968880071408	27.52853416193077	27.615452213217523	22.361044744780294
78-79	22.434928839108554	27.577505404213948	27.596189433584723	22.39137632309277
80-81	22.492535104442663	27.551053122300438	27.570025529306	22.3863862439509
82-83	22.516610564242665	27.52712079832063	27.659129976236574	22.297138661200126
84-85	22.635622687507695	27.47759841789566	27.551510669820185	22.33526822477646
86-87	22.4367250578777	27.51137217666233	27.685959114154407	22.36594365130556
88-89	22.531822438635135	27.567982867761216	27.57784410196135	22.3223505916423
90-91	22.627004579949265	27.507110675259533	27.591606992019884	22.27427775277132
92-93	22.641297630870596	27.502982251977354	27.571701904413214	22.284018212738836
94-95	22.48634861012711	27.57824366944888	27.656004072942185	22.279403647481825
96-97	22.61270515604806	27.64583098433542	27.71414314883005	22.027320710786466
98-99	23.62118222263393	29.615156945788286	27.881243310125654	18.882417521452133
100	22.647178250126636	30.471003468499525	21.813325454251473	25.068492827122363
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	987.0
1	888.0
2	1202.0
3	1803.0
4	2375.0
5	3333.5
6	4210.5
7	5412.0
8	6760.0
9	7384.0
10	7321.0
11	7322.5
12	8247.0
13	10289.5
14	14058.0
15	18507.0
16	21758.5
17	23310.5
18	23318.0
19	21984.5
20	20073.5
21	18904.0
22	19504.5
23	22458.5
24	28032.0
25	36368.0
26	47817.0
27	60518.0
28	72876.5
29	86856.0
30	100907.0
31	115576.5
32	131659.0
33	147161.0
34	163031.0
35	178389.0
36	195061.5
37	211451.0
38	223155.0
39	232705.0
40	244043.5
41	254072.0
42	265790.0
43	294189.0
44	322705.0
45	337388.5
46	334450.5
47	313231.5
48	292855.0
49	280709.0
50	274093.0
51	266784.5
52	256560.0
53	243745.0
54	230238.0
55	215220.5
56	197850.5
57	178461.5
58	158505.5
59	137751.5
60	114519.0
61	92299.0
62	72586.5
63	55402.5
64	41350.5
65	30177.0
66	21978.0
67	16557.0
68	12488.5
69	9124.0
70	6522.5
71	4541.5
72	3164.0
73	2089.0
74	1327.5
75	855.5
76	547.0
77	350.5
78	220.5
79	139.5
80	88.5
81	59.0
82	45.0
83	31.0
84	16.5
85	8.5
86	5.5
87	4.5
88	5.0
89	4.5
90	2.5
91	1.5
92	1.0
93	0.5
94	0.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.010010630822965789
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.3133620580208335E-4
22-23	7.296729532856079E-6
24-25	4.2323486433680735E-4
26-27	0.002816958968730296
28-29	0.007022741564424456
30-31	0.007146336884897138
32-33	0.0023208244798516194
34-35	7.999656528508684E-4
36-37	1.1784779868414094E-4
38-39	4.8121371278441214E-4
40-41	1.0422362518802127E-4
42-43	9.731259297563269E-5
44-45	1.1300135594093705E-4
46-47	1.517712655257262E-5
48-49	9.172443777505866E-4
50-51	3.847520011721085E-4
52-53	0.0
54-55	0.008185110279758658
56-57	0.06641461243723525
58-59	0.07994816424500377
60-61	0.047192937984871876
62-63	0.009691057270300903
64-65	7.279084634078798E-5
66-67	0.0
68-69	6.541348776436571E-4
70-71	0.003547731200735915
72-73	0.013101619167983419
74-75	0.01164926206339376
76-77	0.017373678626340726
78-79	0.0075253084222398296
80-81	0.008413905850150092
82-83	0.04246410852958261
84-85	0.026725554530708996
86-87	0.00982528625593611
88-89	0.0024312295426321996
90-91	9.150564113976498E-6
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	41.0
20-21	197.0
22-23	411.0
24-25	482.0
26-27	1540.0
28-29	4340.0
30-31	12670.0
32-33	15560.0
34-35	21370.0
36-37	32863.0
38-39	37841.0
40-41	36506.0
42-43	40217.0
44-45	47394.0
46-47	48547.0
48-49	44748.0
50-51	40068.0
52-53	37838.0
54-55	40093.0
56-57	40685.0
58-59	43964.0
60-61	53516.0
62-63	55871.0
64-65	63817.0
66-67	74883.0
68-69	71332.0
70-71	90900.0
72-73	50969.0
74-75	44980.0
76-77	46591.0
78-79	47525.0
80-81	47669.0
82-83	44989.0
84-85	44030.0
86-87	45036.0
88-89	46936.0
90-91	48272.0
92-93	47791.0
94-95	57811.0
96-97	203146.0
98-99	462219.0
100-101	4657057.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.07824315892897
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.02106206426545	77.48854869792878
2	7.191073291455307	12.379899107111392
3	1.6271185691132295	4.201785235216113
4	0.5468259826036989	1.8827927958472581
5	0.24178168187624413	1.0406071201959075
6	0.13730579843226012	0.7091425142749785
7	0.07305097669675709	0.44016698145704974
8	0.04484794893253234	0.30883461227149983
9	0.0300871973540557	0.23308677808317815
>10	0.08495281310543129	1.104664891446669
>50	0.0011677044529026666	0.06717500161883036
>100	6.745084052979587E-4	0.09955128811985907
>500	2.5731653585573854E-5	0.017719484273762822
>1k	2.573165321741661E-5	0.026025492154727783
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	8.75565378101964E-5	0.0	0.0	0.0
3	0.0	8.75565378101964E-5	0.0	0.0	0.0
4	0.0	1.6052031931869338E-4	0.0	1.45927563016994E-5	0.0
5	0.0	1.751130756203928E-4	0.0	1.45927563016994E-5	0.0
6	0.0	1.751130756203928E-4	0.0	2.91855126033988E-5	1.45927563016994E-5
7	0.0	1.751130756203928E-4	0.0	2.91855126033988E-5	1.45927563016994E-5
8	0.0	1.897058319220922E-4	0.0	2.91855126033988E-5	1.45927563016994E-5
9	0.0	2.91855126033988E-4	0.0	5.83710252067976E-5	1.45927563016994E-5
10-11	0.0	2.91855126033988E-4	0.0	7.2963781508497E-5	1.45927563016994E-5
12-13	0.0	2.91855126033988E-4	0.0	1.021492941118958E-4	1.45927563016994E-5
14-15	1.45927563016994E-5	3.356333949390862E-4	0.0	1.021492941118958E-4	2.91855126033988E-5
16-17	2.91855126033988E-5	3.502261512407856E-4	0.0	1.167420504135952E-4	3.64818907542485E-5
18-19	2.91855126033988E-5	3.64818907542485E-4	0.0	1.167420504135952E-4	5.1074647055947904E-5
20-21	2.91855126033988E-5	4.085971764475832E-4	0.0	1.167420504135952E-4	5.83710252067976E-5
22-23	2.91855126033988E-5	4.742645798052305E-4	0.0	1.167420504135952E-4	6.56674033576473E-5
24-25	2.91855126033988E-5	5.545247394645773E-4	0.0	1.313348067152946E-4	7.2963781508497E-5
26-27	2.91855126033988E-5	5.691174957662766E-4	0.0	1.970022100729419E-4	8.75565378101964E-5
28-29	2.91855126033988E-5	5.691174957662766E-4	0.0	5.83710252067976E-4	8.75565378101964E-5
30-31	2.91855126033988E-5	6.274885209730742E-4	0.0	0.0019846148570311184	8.75565378101964E-5
32-33	2.91855126033988E-5	6.420812772747735E-4	0.0	0.005267985024913484	8.75565378101964E-5
34-35	2.91855126033988E-5	7.734160839900682E-4	0.0	0.009208029226372321	8.75565378101964E-5
36-37	2.91855126033988E-5	9.193436470070622E-4	0.0	0.015336986873086069	8.75565378101964E-5
38-39	2.91855126033988E-5	9.777146722138598E-4	0.0	0.02962329529244978	8.75565378101964E-5
40-41	2.91855126033988E-5	0.0010214929411189579	0.0	0.04509161697225114	8.75565378101964E-5
42-43	2.91855126033988E-5	0.001043382075571507	0.0	0.05489065282884229	1.0214929411189581E-4
44-45	2.91855126033988E-5	0.0011601241259851023	0.0	0.06503261845852337	1.167420504135952E-4
46-47	2.91855126033988E-5	0.001262273420096998	0.0	0.07645874664275401	1.167420504135952E-4
48-49	2.91855126033988E-5	0.0012695697982478477	0.0	0.08790676396143718	1.167420504135952E-4
50-51	2.91855126033988E-5	0.0013571263360580442	0.0	0.09861055070873369	1.167420504135952E-4
52-53	2.91855126033988E-5	0.0013571263360580442	0.0	0.12323582696785143	1.313348067152946E-4
54-55	2.91855126033988E-5	0.001408200983113992	0.0	0.15218055909227218	1.45927563016994E-4
56-57	2.91855126033988E-5	0.00145927563016994	0.0	0.1916904467791233	1.45927563016994E-4
58-59	2.91855126033988E-5	0.0014957575209241885	0.0	0.21925616343303347	1.45927563016994E-4
60-61	2.91855126033988E-5	0.0015614249242818358	0.0	0.23260123907093758	1.45927563016994E-4
62-63	2.91855126033988E-5	0.0016124995713377838	0.0	0.24312261636446283	1.45927563016994E-4
64-65	2.91855126033988E-5	0.0017803162688073268	0.0	0.2481206353977949	1.45927563016994E-4
66-67	2.91855126033988E-5	0.0017803162688073268	0.0	0.2531551363218812	1.45927563016994E-4
68-69	2.91855126033988E-5	0.001824094537712425	0.0	0.2580510060611013	1.5322394116784369E-4
70-71	3.64818907542485E-5	0.0018751691847683728	0.0	0.2617794552961855	1.6052031931869338E-4
72-73	5.83710252067976E-5	0.001897058319220922	0.0	0.264960676169956	1.6052031931869338E-4
74-75	5.83710252067976E-5	0.0019846148570311184	0.0	0.2673830737160381	1.6052031931869338E-4
76-77	5.83710252067976E-5	0.002094060529293864	0.0	0.2697325074806117	1.6052031931869338E-4
78-79	5.83710252067976E-5	0.002130542420048112	0.0	0.27132311791749697	1.6052031931869338E-4
80-81	5.83710252067976E-5	0.002137838798198962	0.0	0.2720819412451853	1.678166974695431E-4
82-83	5.83710252067976E-5	0.0021524315545006613	0.0	0.2725635022031414	1.751130756203928E-4
84-85	5.83710252067976E-5	0.0021743206889532104	0.0	0.2727532080350635	1.751130756203928E-4
86-87	6.56674033576473E-5	0.0022326917141600082	0.0	0.27284076457287365	1.970022100729419E-4
88	7.2963781508497E-5	0.002334841008271904	0.0	0.2728553573291754	2.042985882237916E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7640	0.0	47.476463	1
CTTATAC	10510	0.0	45.811184	1
TATACAC	13750	0.0	38.641552	3
GTATCAA	14330	0.0	37.8328	1
TTATACA	13850	0.0	35.60238	2
TCAACGC	16780	0.0	31.433214	4
ATCAACG	16825	0.0	31.400286	3
CAACGCA	17205	0.0	30.806782	5
TATCAAC	17560	0.0	30.040703	2
AACGCAG	18020	0.0	29.485088	6
TACACAT	18915	0.0	27.953478	5
ACGCAGA	20540	0.0	25.762913	7
ACACATC	19775	0.0	25.737038	6
CGTATAC	690	0.0	25.56695	1
CGCAGAG	20840	0.0	25.371403	8
ATACACA	21920	0.0	25.259706	4
ACATCTC	21035	0.0	24.215837	8
CACATCT	21920	0.0	23.179264	7
GTGGTAT	3930	0.0	21.458914	1
TGGTATC	3865	0.0	21.26317	2
>>END_MODULE