FastQCFastQC Report
Fri 27 May 2016
SRR2140156_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140156_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6203646
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78900.051.0255851
CTTATAC99400.046.2450561
TATACAC128250.037.7445643
GTATCAA147150.037.5388031
TTATACA133500.035.42932
TCAACGC177200.030.5389444
ATCAACG178300.030.350543
TATCAAC182600.029.898442
CAACGCA181900.029.7026845
AACGCAG188400.028.7918036
TACACAT176950.028.084115
ACACATC183850.025.69966
ACGCAGA216600.024.9640357
ACATCTC189200.024.9275328
ATACACA206200.024.8703524
CGCAGAG218650.024.7496058
CACATCT201650.023.3246427
GTGGTAT38350.023.166861
CATCTCC209050.022.293719
TGGTATC36800.022.159912
Produced by FastQC (version 0.11.5)