##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140156_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6203646
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.559535473171746	32.0	28.0	33.0	18.0	34.0
2	31.136542929754533	33.0	30.0	33.0	27.0	34.0
3	32.36915581578962	33.0	33.0	33.0	30.0	34.0
4	32.462932926862685	33.0	33.0	34.0	31.0	34.0
5	32.93405926127958	33.0	33.0	34.0	32.0	34.0
6	36.75527649385539	38.0	37.0	38.0	34.0	38.0
7	36.96344278832158	38.0	38.0	38.0	35.0	38.0
8	37.16175681204247	38.0	38.0	38.0	36.0	38.0
9	37.20403356348831	38.0	38.0	38.0	36.0	38.0
10-11	37.28179646291874	38.0	38.0	38.0	36.5	38.0
12-13	37.32524994172782	38.0	38.0	38.0	37.0	38.0
14-15	37.32568726519857	38.0	38.0	38.0	37.0	38.0
16-17	37.37242381335105	38.0	38.0	38.0	37.0	38.0
18-19	37.37175646386012	38.0	38.0	38.0	37.0	38.0
20-21	37.39101053824	38.0	38.0	38.0	37.0	38.0
22-23	37.3284120740574	38.0	38.0	38.0	37.0	38.0
24-25	37.396069888397605	38.0	38.0	38.0	37.0	38.0
26-27	37.36017889917378	38.0	38.0	38.0	37.0	38.0
28-29	37.32707995712325	38.0	38.0	38.0	37.0	38.0
30-31	37.339567248297286	38.0	38.0	38.0	37.0	38.0
32-33	37.318609106446345	38.0	38.0	38.0	37.0	38.0
34-35	37.223527356448784	38.0	38.0	38.0	37.0	38.0
36-37	37.23989867157934	38.0	38.0	38.0	37.0	38.0
38-39	37.21476171233655	38.0	38.0	38.0	37.0	38.0
40-41	37.27156044157003	38.0	38.0	38.0	37.0	38.0
42-43	37.25534563288295	38.0	38.0	38.0	37.0	38.0
44-45	37.22139248782253	38.0	38.0	38.0	37.0	38.0
46-47	37.19208080438598	38.0	38.0	38.0	37.0	38.0
48-49	37.135861561191305	38.0	38.0	38.0	36.5	38.0
50-51	37.09731091727472	38.0	38.0	38.0	36.0	38.0
52-53	37.00869414534329	38.0	38.0	38.0	36.0	38.0
54-55	37.04943463313574	38.0	38.0	38.0	36.0	38.0
56-57	37.01851825942828	38.0	38.0	38.0	36.0	38.0
58-59	36.99528321990071	38.0	38.0	38.0	36.0	38.0
60-61	36.98800422268652	38.0	38.0	38.0	36.0	38.0
62-63	37.00033207110742	38.0	38.0	38.0	36.0	38.0
64-65	37.00987274720998	38.0	38.0	38.0	36.0	38.0
66-67	37.00545854872598	38.0	38.0	38.0	36.0	38.0
68-69	36.988104322434076	38.0	38.0	38.0	36.0	38.0
70-71	36.99056467298452	38.0	38.0	38.0	36.0	38.0
72-73	37.00161468611512	38.0	38.0	38.0	36.0	38.0
74-75	36.953218004164555	38.0	38.0	38.0	36.0	38.0
76-77	36.96763762690006	38.0	38.0	38.0	36.0	38.0
78-79	36.98217193552655	38.0	38.0	38.0	36.0	38.0
80-81	36.97485229505594	38.0	38.0	38.0	36.0	38.0
82-83	36.93546456714401	38.0	38.0	38.0	36.0	38.0
84-85	36.936909728829335	38.0	38.0	38.0	36.0	38.0
86-87	36.9358456477312	38.0	38.0	38.0	36.0	38.0
88-89	36.925822352277706	38.0	38.0	38.0	36.0	38.0
90-91	36.93975120538201	38.0	38.0	38.0	36.0	38.0
92-93	36.92385672519891	38.0	38.0	38.0	35.0	38.0
94-95	36.861564566731225	38.0	38.0	38.0	35.0	38.0
96-97	36.89588210628523	38.0	38.0	38.0	35.0	38.0
98-99	36.923624722398	38.0	38.0	38.0	35.0	38.0
100	35.49955025911577	38.0	36.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	11.0
19	14.0
20	54.0
21	131.0
22	440.0
23	1273.0
24	3169.0
25	6535.0
26	11685.0
27	19039.0
28	28818.0
29	41451.0
30	54971.0
31	70600.0
32	88853.0
33	118274.0
34	172504.0
35	301339.0
36	828145.0
37	4456338.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.91368946583992	21.532756704686243	14.108574215872407	24.44497961360142
2	14.431588033649517	20.479253130095707	42.951065420765666	22.13809341548911
3	17.68063490405481	26.947717519665048	30.945221568090766	24.426426008189377
4	12.444697843816362	18.66454017524533	38.48446542565453	30.40629655528378
5	13.164323044867485	37.53550734519668	36.08402220242741	13.216147407508425
6	29.128193323732525	38.41771435700876	18.75933926597359	13.694753053285117
7	25.707398520160563	33.11373666389088	22.923084263673328	18.255780552275226
8	23.78307853156031	37.44236856841928	21.494247092758034	17.280305807262376
9	24.98285685546854	17.25461123990634	21.009467658212603	36.753064246412514
10-11	23.866545576585125	26.91917785121846	29.11283138979884	20.101445182397576
12-13	24.968188062310453	24.198302095251726	29.06053150034673	21.772978342091086
14-15	22.11928275726887	25.734116679127084	26.292248139239412	25.85435242436464
16-17	21.80493535575692	29.25802020295807	26.901551120099377	22.03549332118564
18-19	21.88045546119169	27.920403582022573	28.73423951012034	21.464901446665397
20-21	22.52806851538711	27.10955143570557	28.588167780810082	21.77421226809724
22-23	22.152419893182852	27.60599944126612	28.482144454791964	21.759436210759063
24-25	22.142201285029937	27.875447744886035	28.617932236591958	21.364418733492073
26-27	22.064584095653125	28.192704124955227	28.59931230189258	21.143399477499063
28-29	21.912521586063374	27.864775238013213	28.661082636135944	21.56162053978747
30-31	22.659106727306966	27.45141166103992	28.594095521303352	21.295386090349762
32-33	22.09495565085679	27.75393090635398	28.774844204692734	21.3762692380965
34-35	22.056926759013187	27.324536342497474	29.003534519719476	21.615002378769866
36-37	21.78768478286415	27.771599633827392	28.8706948309794	21.570020752329057
38-39	21.767265440024453	27.117714989984123	29.248747708525226	21.8662718614662
40-41	21.958212348554834	26.98927247066512	29.305950688260285	21.746564492519756
42-43	22.240237898385747	26.932841739259217	29.212982262084257	21.613938100270776
44-45	21.799438068675947	27.38983449031112	29.046803152498953	21.763924288513984
46-47	22.440893466412028	26.912257384418957	28.41515965151336	22.231689497655655
48-49	22.16170191061632	26.99665375718062	28.567984395797374	22.27365993640568
50-51	21.795376019729908	27.333689410931942	28.836332908687694	22.034601660650452
52-53	22.004105367739655	27.422911833338738	29.06131962013754	21.51166317878407
54-55	21.896304638219082	27.470691223488863	28.569239226686538	22.063764911605514
56-57	22.309770557151968	27.968749945819877	27.953570842806908	21.767908654221245
58-59	21.610570138793804	28.551596394211288	28.613377031818903	21.224456435176002
60-61	22.073769963788763	29.095500577119864	27.66807494004395	21.16265451904742
62-63	21.55817639888839	29.573460438891786	27.64208743233203	21.226275729887796
64-65	21.552393365575682	29.43632270803495	27.695862411178062	21.31542151521131
66-67	21.707252125271104	29.36246118088968	27.591484841484526	21.33880185235469
68-69	21.835119301082443	28.875852884973042	27.843373530392622	21.445654283551892
70-71	21.970046795267777	28.211253999173007	28.080809149836767	21.737890055722445
72-73	22.20726877096002	27.918526666956694	28.08526518175506	21.788939380328227
74-75	21.971432447107432	28.009664119231083	28.081658653763945	21.93724477989754
76-77	22.189184805658144	27.828399419715772	27.973154334125955	22.009261440500126
78-79	22.177578316847324	27.858799113639687	27.968482431818174	21.99514013769481
80-81	22.183454608175644	27.84938791701493	27.909305642366323	22.057851832443106
82-83	22.22937894863048	27.755276999300428	28.012948705154113	22.00239534691498
84-85	22.346252532636495	27.728284557900114	27.978082925705255	21.94737998375814
86-87	22.149797203599768	27.78538713238924	28.04995211368827	22.014863550322726
88-89	22.275531282819006	27.802017803866114	27.949366632718604	21.97308428059628
90-91	22.32804984132157	27.75015796331778	28.014218486381953	21.9075737089787
92-93	22.371095896658385	27.757468223716426	27.911239578928775	21.960196300696413
94-95	22.181127315562875	27.904979828411186	27.91464342677244	21.999249429253496
96-97	22.251084312110493	27.90751435301785	28.076391434688148	21.76500990018351
98-99	23.28553412766935	29.87751161018059	28.280825670174636	18.556128591975426
100	22.20116072884273	30.80805733371448	22.299971269564935	24.69081066787786
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1070.0
1	993.5
2	1271.0
3	1793.0
4	2373.0
5	3372.5
6	4244.5
7	5347.5
8	6675.0
9	7354.0
10	7338.0
11	7411.5
12	8258.0
13	10210.5
14	14042.5
15	18462.5
16	21746.0
17	23566.0
18	23641.0
19	22200.0
20	20299.0
21	18927.0
22	19325.0
23	22281.0
24	27751.0
25	35963.0
26	47120.5
27	59882.0
28	72131.5
29	85837.5
30	99648.5
31	113150.0
32	128539.5
33	143382.0
34	158146.5
35	172281.5
36	187886.0
37	203462.0
38	214428.5
39	222855.0
40	233692.0
41	242963.0
42	250819.0
43	273289.0
44	299992.0
45	308522.5
46	296628.0
47	278858.5
48	264283.5
49	252534.0
50	244650.5
51	235738.5
52	222945.5
53	207992.0
54	193311.5
55	177455.5
56	160854.5
57	143905.0
58	126619.5
59	109694.0
60	91204.5
61	72970.0
62	56853.0
63	43517.0
64	32537.0
65	23496.0
66	17095.0
67	12891.0
68	9743.5
69	7060.0
70	5207.5
71	3770.0
72	2589.0
73	1802.0
74	1243.5
75	818.0
76	561.0
77	363.5
78	229.0
79	148.5
80	105.0
81	70.5
82	44.0
83	30.5
84	23.5
85	17.0
86	12.0
87	8.0
88	3.5
89	1.0
90	0.0
91	0.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.018053899271492924
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	3.7079349889370533E-4
26-27	2.4184574739647007E-4
28-29	0.0036610494019103258
30-31	0.006206563137857533
32-33	0.004253119129219947
34-35	0.0021720493236740894
36-37	0.0
38-39	0.0
40-41	2.876423583123053E-4
42-43	8.264492179600307E-6
44-45	0.0
46-47	2.5146659509368553E-4
48-49	0.0015284426714509455
50-51	0.002780627377563959
52-53	0.0
54-55	0.0029616775285903046
56-57	0.03987799862705995
58-59	0.06296348301246973
60-61	0.04340154841665276
62-63	0.01407859832162172
64-65	3.7584608995231854E-4
66-67	0.0
68-69	1.8318710575272542E-5
70-71	0.0029892524306706957
72-73	0.005065837049535977
74-75	0.004692390974575409
76-77	0.0034487076111923204
78-79	0.00841936601110641
80-81	0.0164502714441143
82-83	0.044057171121932505
84-85	0.020087145627534053
86-87	0.002215100046346709
88-89	0.0014770072959102174
90-91	9.194299452612275E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	39.0
20-21	209.0
22-23	399.0
24-25	499.0
26-27	1387.0
28-29	3475.0
30-31	10899.0
32-33	13431.0
34-35	18219.0
36-37	28900.0
38-39	33873.0
40-41	33524.0
42-43	37623.0
44-45	44624.0
46-47	44700.0
48-49	42098.0
50-51	37710.0
52-53	35435.0
54-55	37166.0
56-57	39349.0
58-59	43235.0
60-61	48734.0
62-63	48142.0
64-65	57277.0
66-67	66458.0
68-69	66215.0
70-71	87236.0
72-73	48062.0
74-75	44131.0
76-77	45929.0
78-79	48085.0
80-81	47742.0
82-83	44851.0
84-85	44219.0
86-87	45705.0
88-89	47267.0
90-91	48990.0
92-93	48398.0
94-95	57157.0
96-97	186901.0
98-99	412962.0
100-101	4152391.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.20077300123556
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.16547097035445	77.7233329566502
2	7.062943185126147	12.176623244433653
3	1.6118873405640204	4.168378042425732
4	0.5685094246117459	1.9602380744008059
5	0.24275534706586066	1.0462849283630204
6	0.12530610897549743	0.6480890073278964
7	0.0678789086102028	0.40958500748757937
8	0.04070202154151161	0.28068365756729935
9	0.02492711418510119	0.1933862860301208
>10	0.08733522125344853	1.1215364766792084
>50	0.001411002261782571	0.08071667384037554
>100	7.886498629207199E-4	0.13224536933212327
>500	5.647039288166567E-5	0.03409887938830678
>1k	2.8235194493082243E-5	0.024801396073756535
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.6119552920975826E-5	0.0	0.0	0.0
3	0.0	1.6119552920975826E-5	0.0	0.0	0.0
4	0.0	1.6119552920975826E-5	0.0	0.0	0.0
5	0.0	1.6119552920975826E-5	0.0	0.0	0.0
6	0.0	1.6119552920975826E-5	0.0	0.0	0.0
7	1.6119552920975826E-5	1.6119552920975826E-5	0.0	0.0	0.0
8	1.6119552920975826E-5	6.44782116839033E-5	0.0	0.0	0.0
9	3.223910584195165E-5	1.773150821307341E-4	0.0	3.223910584195165E-5	0.0
10-11	4.0298882302439564E-5	1.773150821307341E-4	0.0	6.44782116839033E-5	0.0
12-13	4.835865876292748E-5	1.773150821307341E-4	0.0	6.44782116839033E-5	0.0
14-15	6.44782116839033E-5	3.1433128195902866E-4	0.0	7.253798814439122E-5	8.059776460487913E-6
16-17	6.44782116839033E-5	3.3851061134049237E-4	0.0	8.059776460487913E-5	2.417932938146374E-5
18-19	6.44782116839033E-5	3.7074971718244404E-4	0.0	1.0477709398634287E-4	3.223910584195165E-5
20-21	6.44782116839033E-5	4.0298882302439566E-4	0.0	1.208966469073187E-4	3.223910584195165E-5
22-23	8.059776460487913E-5	4.271681524058594E-4	0.0	1.4507597628878245E-4	4.835865876292748E-5
24-25	9.671731752585496E-5	4.997061405502506E-4	0.0	1.6925530567024619E-4	7.253798814439122E-5
26-27	9.671731752585496E-5	5.48064799313178E-4	0.0	2.4179329381463738E-4	9.671731752585496E-5
28-29	9.671731752585496E-5	5.48064799313178E-4	0.0	5.964234580761056E-4	9.671731752585496E-5
30-31	9.671731752585496E-5	6.286625639180572E-4	0.0	0.0018215094800702685	9.671731752585496E-5
32-33	9.671731752585496E-5	6.286625639180572E-4	0.0	0.004320040182821522	9.671731752585496E-5
34-35	9.671731752585496E-5	7.41499434364888E-4	0.0	0.007576189872858638	9.671731752585496E-5
36-37	9.671731752585496E-5	8.543363048117188E-4	0.0	0.01347594624193579	9.671731752585496E-5
38-39	9.671731752585496E-5	8.704558577326946E-4	0.0	0.028362353364456964	1.0477709398634287E-4
40-41	1.289564233678066E-4	0.0010477709398634288	0.0	0.04488489510845719	1.289564233678066E-4
42-43	1.289564233678066E-4	0.0010719502692448923	0.0	0.0548145397077783	1.289564233678066E-4
44-45	1.4507597628878245E-4	0.0011525480338497715	0.0	0.06534060776517552	1.3701619982829454E-4
46-47	1.773150821307341E-4	0.0012089664690731869	0.0	0.07680966966844982	1.4507597628878245E-4
48-49	1.9343463505170993E-4	0.001217026245533675	0.0	0.08779514498409484	1.773150821307341E-4
50-51	2.0955418797268574E-4	0.0014346402099668484	0.0	0.09929644599321108	1.773150821307341E-4
52-53	2.0955418797268574E-4	0.0014346402099668484	0.0	0.12919015688516075	1.9343463505170993E-4
54-55	2.1761396443317364E-4	0.0014426999864273365	0.0	0.16589437888622272	2.0955418797268574E-4
56-57	2.2567374089366157E-4	0.0014507597628878243	0.0	0.21656619349331024	2.0955418797268574E-4
58-59	2.3373351735414948E-4	0.0014588195393483122	0.0	0.2513118898144736	2.0955418797268574E-4
60-61	2.4179329381463738E-4	0.001482998868729776	0.0	0.26562605280830015	2.2567374089366157E-4
62-63	2.4179329381463738E-4	0.001482998868729776	0.0	0.27594256667772465	2.2567374089366157E-4
64-65	2.498530702751253E-4	0.0016441943979395343	0.0	0.2809154487538457	2.2567374089366157E-4
66-67	2.579128467356132E-4	0.0016603139508605102	0.0	0.28577549395951996	2.2567374089366157E-4
68-69	2.579128467356132E-4	0.0016844932802419737	0.0	0.2904985229653658	2.2567374089366157E-4
70-71	2.74032399656589E-4	0.0017167323860839256	0.0	0.295044236889081	2.2567374089366157E-4
72-73	2.74032399656589E-4	0.0017247921625444134	0.0	0.2983890441201835	2.4179329381463738E-4
74-75	2.74032399656589E-4	0.0017328519390049013	0.0	0.30121802565781475	2.4179329381463738E-4
76-77	2.74032399656589E-4	0.0018376290329912442	0.0	0.3040228278660646	2.4179329381463738E-4
78-79	2.74032399656589E-4	0.0018859876917541717	0.0	0.30571538092276707	2.4179329381463738E-4
80-81	2.74032399656589E-4	0.0018940474682146596	0.0	0.30631180438084316	2.4179329381463738E-4
82-83	2.74032399656589E-4	0.0019021072446751474	0.0	0.30677927141555145	2.4179329381463738E-4
84-85	2.901519525775649E-4	0.0019424061269775871	0.0	0.30698882560352414	2.4179329381463738E-4
86-87	3.062715054985407E-4	0.0019988245622010025	0.0	0.3070774831445895	2.4179329381463738E-4
88	3.062715054985407E-4	0.0020955418797268576	0.0	0.3070774831445895	2.4179329381463738E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7890	0.0	51.025585	1
CTTATAC	9940	0.0	46.245056	1
TATACAC	12825	0.0	37.744564	3
GTATCAA	14715	0.0	37.538803	1
TTATACA	13350	0.0	35.4293	2
TCAACGC	17720	0.0	30.538944	4
ATCAACG	17830	0.0	30.35054	3
TATCAAC	18260	0.0	29.89844	2
CAACGCA	18190	0.0	29.702684	5
AACGCAG	18840	0.0	28.791803	6
TACACAT	17695	0.0	28.08411	5
ACACATC	18385	0.0	25.6996	6
ACGCAGA	21660	0.0	24.964035	7
ACATCTC	18920	0.0	24.927532	8
ATACACA	20620	0.0	24.870352	4
CGCAGAG	21865	0.0	24.749605	8
CACATCT	20165	0.0	23.324642	7
GTGGTAT	3835	0.0	23.16686	1
CATCTCC	20905	0.0	22.29371	9
TGGTATC	3680	0.0	22.15991	2
>>END_MODULE