FastQCFastQC Report
Fri 27 May 2016
SRR2140155_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140155_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5086639
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83800.052.1192131
GTATCAA155050.038.794031
CTTATAC93950.034.345271
ATCAACG187650.031.5960673
TCAACGC187850.031.5850834
TATCAAC192100.031.1789322
CAACGCA192300.030.8320455
AACGCAG197800.030.0392866
GTGGTAT36700.028.4180681
TATACAC123100.027.8335323
TGGTATC36150.026.9663182
ACGCAGA223150.026.5505087
TTATACA127100.026.2916982
CGCAGAG226550.026.1520448
GCAGAGT259550.022.7941939
TACACAT152650.022.7801065
ACACATC149500.021.9789316
ACATCTC153550.021.4546598
GAGTACT141800.021.3114312-13
CACATCT158650.020.5503467
Produced by FastQC (version 0.11.5)