##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140155_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5086639
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.74077381941199	33.0	28.0	33.0	18.0	34.0
2	31.164359806150976	33.0	31.0	33.0	27.0	34.0
3	32.364440448791434	33.0	33.0	33.0	30.0	34.0
4	32.43776784631266	33.0	33.0	34.0	31.0	34.0
5	32.90548297215509	33.0	33.0	34.0	32.0	34.0
6	36.7188019436803	38.0	37.0	38.0	34.0	38.0
7	36.924838188831565	38.0	38.0	38.0	35.0	38.0
8	37.13389548580113	38.0	38.0	38.0	36.0	38.0
9	37.1793530069659	38.0	38.0	38.0	36.0	38.0
10-11	37.2612968995834	38.0	38.0	38.0	36.0	38.0
12-13	37.3076726105391	38.0	38.0	38.0	36.5	38.0
14-15	37.30935092897295	38.0	38.0	38.0	37.0	38.0
16-17	37.35738323478431	38.0	38.0	38.0	37.0	38.0
18-19	37.360631450354546	38.0	38.0	38.0	37.0	38.0
20-21	37.37763001157903	38.0	38.0	38.0	37.0	38.0
22-23	37.30916856979633	38.0	38.0	38.0	37.0	38.0
24-25	37.38199174526834	38.0	38.0	38.0	37.0	38.0
26-27	37.34599484457762	38.0	38.0	38.0	37.0	38.0
28-29	37.30219740385386	38.0	38.0	38.0	37.0	38.0
30-31	37.3192044670206	38.0	38.0	38.0	37.0	38.0
32-33	37.30639835481785	38.0	38.0	38.0	37.0	38.0
34-35	37.215218750369075	38.0	38.0	38.0	37.0	38.0
36-37	37.23028342828766	38.0	38.0	38.0	37.0	38.0
38-39	37.200442534997634	38.0	38.0	38.0	37.0	38.0
40-41	37.25789903456099	38.0	38.0	38.0	37.0	38.0
42-43	37.23763496399035	38.0	38.0	38.0	37.0	38.0
44-45	37.19428312060826	38.0	38.0	38.0	37.0	38.0
46-47	37.152247824344386	38.0	38.0	38.0	37.0	38.0
48-49	37.08948155249986	38.0	38.0	38.0	36.0	38.0
50-51	37.049309642784664	38.0	38.0	38.0	36.0	38.0
52-53	36.956524198762494	38.0	38.0	38.0	36.0	38.0
54-55	36.998811491782305	38.0	38.0	38.0	36.0	38.0
56-57	36.96783349585401	38.0	38.0	38.0	36.0	38.0
58-59	36.9459250054207	38.0	38.0	38.0	36.0	38.0
60-61	36.941106005138124	38.0	38.0	38.0	36.0	38.0
62-63	36.953512876929295	38.0	38.0	38.0	36.0	38.0
64-65	36.96846653786571	38.0	38.0	38.0	36.0	38.0
66-67	36.96948162668885	38.0	38.0	38.0	36.0	38.0
68-69	36.96138153024546	38.0	38.0	38.0	36.0	38.0
70-71	36.974580317464415	38.0	38.0	38.0	36.0	38.0
72-73	36.99403128201914	38.0	38.0	38.0	36.0	38.0
74-75	36.945949988398226	38.0	38.0	38.0	36.0	38.0
76-77	36.96156224681485	38.0	38.0	38.0	36.0	38.0
78-79	36.97135398794536	38.0	38.0	38.0	36.0	38.0
80-81	36.955724755002365	38.0	38.0	38.0	36.0	38.0
82-83	36.917436117848496	38.0	38.0	38.0	36.0	38.0
84-85	36.92081165092507	38.0	38.0	38.0	36.0	38.0
86-87	36.92177325980686	38.0	38.0	38.0	36.0	38.0
88-89	36.907204438083426	38.0	38.0	38.0	35.0	38.0
90-91	36.92165945621587	38.0	38.0	38.0	35.5	38.0
92-93	36.906959748938014	38.0	38.0	38.0	35.0	38.0
94-95	36.8375259864128	38.0	38.0	38.0	35.0	38.0
96-97	36.87312980002343	38.0	38.0	38.0	35.0	38.0
98-99	36.901906227924854	38.0	38.0	38.0	35.0	38.0
100	35.43354949018448	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	12.0
20	32.0
21	116.0
22	347.0
23	1070.0
24	2612.0
25	5389.0
26	9813.0
27	16042.0
28	24726.0
29	35657.0
30	47080.0
31	60706.0
32	76516.0
33	100422.0
34	145053.0
35	251689.0
36	691047.0
37	3618306.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.325035843904	21.528518143316244	14.431199068776063	23.71524694400369
2	14.4524331769779	20.74393856421076	42.97878239393901	21.82484586487233
3	17.65890994033585	27.45842588789965	30.964984934059604	23.9176792377049
4	12.196894648902742	18.85783520316657	38.875788118637864	30.06948202929282
5	12.9165053781092	37.97114361762256	35.85212160721451	13.260229397053733
6	28.585987721951568	38.7817378036853	18.974572404292893	13.657702070070238
7	25.636495925895268	33.70604833564953	22.81182132248819	17.84563441596701
8	23.989652106233606	37.30534052052839	21.349165922724218	17.355841450513786
9	25.29747049082901	17.359321154892257	21.071399012196462	36.271809342082264
10-11	23.687065270407434	27.351724783299936	28.83951465791065	20.121695288381975
12-13	24.90222915367102	24.83159312072274	28.883964440959936	21.382213284646305
14-15	22.160713587105356	26.241227262245264	26.548699445744035	25.04935970490534
16-17	21.428687980413	29.70188173369488	27.188955221709264	21.680475064182854
18-19	21.408841869847652	28.142993831486763	29.26708579083359	21.181078507831987
20-21	22.43685262780534	27.23734356733467	29.11426523179111	21.211538573068882
22-23	22.099010473288104	27.52400286218692	29.001898327091265	21.375088337433713
24-25	22.00375038120224	27.622499377197627	29.27993034837805	21.093819893222083
26-27	21.895743134037996	27.846197652945264	29.216007733713884	21.042051479302856
28-29	21.378754238205882	27.50429513168573	29.397566162536027	21.719384467572358
30-31	22.169999877911565	27.443403148227567	29.393785619793768	20.9928113540671
32-33	22.184570451814007	27.339430841772263	29.615785540917756	20.860213165495974
34-35	21.364288736269632	27.55392510208222	30.004501816306306	21.077284345341837
36-37	21.828331492520327	27.581032681200252	29.395522822340723	21.195113003938697
38-39	21.4286691626573	27.412365091113777	29.70870377108183	21.450261975147093
40-41	21.500112317709867	27.323556918444275	29.885154513488427	21.29117625035743
42-43	21.797493116793003	27.198013833172507	29.82064213594865	21.183850914085845
44-45	21.40726920722628	27.725178136568278	29.526346396919482	21.34120625928596
46-47	21.99226439650345	27.247150866065567	28.8525178753967	21.908066862034286
48-49	21.80354404277289	27.388116093410375	29.057426935949053	21.750912927867677
50-51	21.51509830634466	27.79855134317267	29.039826925029743	21.646523425452926
52-53	21.60348760312819	28.047244356588163	29.170765178997105	21.17850286128654
54-55	21.572011763481765	28.149901131302276	28.73409777368036	21.5439893315356
56-57	21.925179820124825	28.504422694796805	28.152430726400723	21.417966758677647
58-59	21.25079272443059	28.9409244646326	28.749799061706938	21.058483749229868
60-61	21.821424932304552	29.367857831967864	28.04766106530633	20.76305617042126
62-63	21.205477628623225	29.676209792226103	28.03919141060683	21.079121168543843
64-65	21.168256544847473	29.49907012129756	28.188409737224724	21.144263596630246
66-67	21.320144235824927	29.563058249177704	28.15541011181774	20.96138740317963
68-69	21.472255054999255	29.298431886946517	28.182665886151586	21.046647171902634
70-71	21.582530153343495	28.58629411747817	28.259325931731855	21.571849797446475
72-73	21.783153682440464	28.387034849947423	28.255548032001805	21.574263435610305
74-75	21.558480536634285	28.51077211918772	28.472032499882687	21.458714844295308
76-77	21.75103029540378	28.024248669356343	28.439809557841077	21.784911477398794
78-79	21.76830202632853	28.0731905999612	28.20991220226212	21.948595171448147
80-81	21.996324169440015	28.277134024862434	28.11376781597248	21.61277398972507
82-83	22.026491477011866	27.935359228982687	28.300189399736286	21.737959894269157
84-85	21.976775058625993	27.934278567780453	28.165062022667236	21.923884350926322
86-87	21.763913779246515	28.271404182245675	28.144708795295596	21.819973243212214
88-89	22.085213031317423	28.221008730658376	28.15717076385034	21.536607474173866
90-91	22.186109358368867	28.147485292899166	28.225001115381428	21.441404233350543
92-93	21.990616621983914	27.951059496803467	28.090379459682406	21.967944421530213
94-95	21.978707289180242	28.328526242595252	28.094862647503323	21.59790382072118
96-97	22.297970633657123	28.17639143115227	28.264535492057103	21.261102443133506
98-99	22.954084965654232	30.23385394220594	28.580793799164756	18.231267292975076
100	21.85075543820384	31.196413932998446	22.573052149683217	24.379778479114496
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1343.0
1	1158.0
2	1291.0
3	1793.0
4	2388.5
5	3377.0
6	4211.5
7	5189.5
8	6390.5
9	7189.0
10	7326.0
11	7326.0
12	8301.5
13	10649.5
14	14917.0
15	19786.5
16	23402.5
17	25364.0
18	25171.5
19	23133.5
20	20456.5
21	18215.0
22	17704.0
23	19811.0
24	24457.0
25	31307.0
26	41022.0
27	52122.0
28	62300.5
29	73711.5
30	85137.0
31	96439.0
32	110562.0
33	125225.0
34	139548.5
35	153042.0
36	168063.5
37	181109.5
38	187404.0
39	192354.0
40	199252.0
41	204147.5
42	206467.5
43	210391.0
44	213662.0
45	218396.5
46	237696.0
47	253180.0
48	249338.0
49	235731.5
50	216116.5
51	196233.0
52	178621.0
53	164752.5
54	151994.0
55	138190.0
56	124002.5
57	109488.5
58	95514.0
59	83030.0
60	68855.0
61	54216.5
62	41474.0
63	31147.5
64	23517.5
65	17092.5
66	12066.0
67	8801.0
68	6562.0
69	4722.5
70	3349.5
71	2352.0
72	1663.0
73	1180.0
74	790.5
75	523.0
76	326.5
77	204.5
78	144.5
79	91.0
80	57.0
81	39.0
82	28.0
83	18.5
84	11.0
85	6.5
86	4.5
87	3.0
88	1.0
89	0.0
90	0.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.019737984158105185
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	4.0307489121647704E-4
26-27	3.3429794678134E-4
28-29	0.003983417965392064
30-31	0.006320630834400064
32-33	0.004508406599873192
34-35	0.00208715318636082
36-37	0.0
38-39	0.0
40-41	4.522840343735866E-4
42-43	0.0
44-45	0.0
46-47	2.9817086568358704E-4
48-49	0.0013588061425505891
50-51	0.0026562499836598795
52-53	0.0
54-55	0.0031606437488883392
56-57	0.0408703274797764
58-59	0.06531202459534287
60-61	0.0448139160644887
62-63	0.01425042595311216
64-65	4.199577168888206E-4
66-67	0.0
68-69	1.1320561626646971E-5
70-71	0.002848013791736465
72-73	0.005288081026446811
74-75	0.004504130511121204
76-77	0.0034426084525534363
78-79	0.008344321221838815
80-81	0.016618553209591328
82-83	0.04624502963830366
84-85	0.02086022217619572
86-87	0.0019093798346776574
88-89	0.0015760655905542984
90-91	5.098883930790809E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	38.0
20-21	181.0
22-23	444.0
24-25	496.0
26-27	1241.0
28-29	3358.0
30-31	9730.0
32-33	12572.0
34-35	17395.0
36-37	27151.0
38-39	31554.0
40-41	31063.0
42-43	35408.0
44-45	41973.0
46-47	43222.0
48-49	40245.0
50-51	36025.0
52-53	32146.0
54-55	34112.0
56-57	36979.0
58-59	23825.0
60-61	47869.0
62-63	44176.0
64-65	49028.0
66-67	55522.0
68-69	57298.0
70-71	69725.0
72-73	42505.0
74-75	39360.0
76-77	40939.0
78-79	42301.0
80-81	42772.0
82-83	40199.0
84-85	39278.0
86-87	40467.0
88-89	42703.0
90-91	43353.0
92-93	43699.0
94-95	49377.0
96-97	167277.0
98-99	328635.0
100-101	3300998.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.95078239021085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.4385932541003	78.63706441714065
2	6.979934047027292	12.138214528421877
3	1.5673038023792298	4.0883487558007925
4	0.5151572780615805	1.791733135258633
5	0.21553425977046617	0.937043625946849
6	0.1085294299816144	0.5662031309558988
7	0.05284781412562482	0.32166111500848604
8	0.034305707097650115	0.23863264580720692
9	0.020425859193859093	0.15984399941084965
>10	0.06482255693336497	0.8141873388033645
>50	0.0013552044821432284	0.08523992486088497
>100	0.00115809504018252	0.1970401008731592
>500	3.269180678736456E-5	0.02478728171138352
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.96593467710211E-5
7	0.0	0.0	0.0	9.82967338551055E-5	1.96593467710211E-5
8	0.0	0.0	0.0	9.82967338551055E-5	1.96593467710211E-5
9	0.0	5.89780403130633E-5	0.0	1.179560806261266E-4	1.96593467710211E-5
10-11	0.0	5.89780403130633E-5	0.0	1.376154273971477E-4	1.96593467710211E-5
12-13	0.0	5.89780403130633E-5	0.0	1.376154273971477E-4	2.948902015653165E-5
14-15	0.0	1.0812640724061605E-4	0.0	1.376154273971477E-4	4.914836692755275E-5
16-17	1.96593467710211E-5	1.179560806261266E-4	0.0	1.376154273971477E-4	5.89780403130633E-5
18-19	1.96593467710211E-5	1.4744510078265827E-4	0.0	1.572747741681688E-4	5.89780403130633E-5
20-21	1.96593467710211E-5	1.96593467710211E-4	0.0	2.162528144812321E-4	5.89780403130633E-5
22-23	1.96593467710211E-5	2.2608248786674267E-4	0.0	2.7523085479429536E-4	6.880771369857385E-5
24-25	2.948902015653165E-5	2.555715080232743E-4	0.0	3.7352758864940094E-4	9.82967338551055E-5
26-27	3.93186935420422E-5	2.752308547942954E-4	0.0	4.718243225045064E-4	9.82967338551055E-5
28-29	3.93186935420422E-5	2.8506052817980595E-4	0.0	8.256925643828862E-4	9.82967338551055E-5
30-31	3.93186935420422E-5	3.538682418783798E-4	0.0	0.001857808269861494	9.82967338551055E-5
32-33	3.93186935420422E-5	3.538682418783798E-4	0.0	0.004148122168685452	9.82967338551055E-5
34-35	3.93186935420422E-5	4.128462821914431E-4	0.0	0.00697906810371249	9.82967338551055E-5
36-37	3.93186935420422E-5	4.718243225045064E-4	0.0	0.012287091731888189	9.82967338551055E-5
38-39	3.93186935420422E-5	4.718243225045064E-4	0.0	0.02840775608412549	1.0812640724061605E-4
40-41	3.93186935420422E-5	4.914836692755275E-4	0.0	0.04614048687158652	1.179560806261266E-4
42-43	3.93186935420422E-5	4.914836692755275E-4	0.0	0.05480042912422132	1.2778575401163714E-4
44-45	3.93186935420422E-5	5.111430160465486E-4	0.0	0.06524937193301902	1.4744510078265827E-4
46-47	3.93186935420422E-5	5.308023628175697E-4	0.0	0.07585558951598492	1.572747741681688E-4
48-49	3.93186935420422E-5	5.308023628175697E-4	0.0	0.08689431272791326	1.572747741681688E-4
50-51	3.93186935420422E-5	6.192694232871646E-4	0.0	0.09783473920598651	1.572747741681688E-4
52-53	3.93186935420422E-5	6.290990966726752E-4	0.0	0.1314522221844326	1.572747741681688E-4
54-55	3.93186935420422E-5	6.389287700581858E-4	0.0	0.17274668007696242	1.572747741681688E-4
56-57	5.89780403130633E-5	6.684177902147174E-4	0.0	0.2242738279638087	1.572747741681688E-4
58-59	7.86373870840844E-5	6.684177902147174E-4	0.0	0.4256248575926068	1.572747741681688E-4
60-61	7.86373870840844E-5	6.979068103712491E-4	0.0	0.6049574188378612	1.572747741681688E-4
62-63	7.86373870840844E-5	7.175661571422701E-4	0.0	0.611150113070733	1.572747741681688E-4
64-65	7.86373870840844E-5	8.256925643828862E-4	0.0	0.616831664287558	1.572747741681688E-4
66-67	7.86373870840844E-5	8.748409313104389E-4	0.0	0.6222576439963599	1.572747741681688E-4
68-69	7.86373870840844E-5	9.239892982379917E-4	0.0	0.627418222523753	1.572747741681688E-4
70-71	7.86373870840844E-5	9.436486450090128E-4	0.0	0.632116806402027	1.572747741681688E-4
72-73	7.86373870840844E-5	9.436486450090128E-4	0.0	0.6359012306554486	1.769341209391899E-4
74-75	7.86373870840844E-5	9.534783183945234E-4	0.0	0.6391941712395945	1.769341209391899E-4
76-77	7.86373870840844E-5	0.0010321157054786077	0.0	0.642398644763271	1.769341209391899E-4
78-79	7.86373870840844E-5	0.0010812640724061605	0.0	0.6440991782589643	1.769341209391899E-4
80-81	7.86373870840844E-5	0.001091093745791671	0.0	0.6447970850693356	1.769341209391899E-4
82-83	7.86373870840844E-5	0.0011205827659482026	0.0	0.6452492500450691	1.769341209391899E-4
84-85	7.86373870840844E-5	0.001179560806261266	0.0	0.6454556731861648	1.769341209391899E-4
86-87	7.86373870840844E-5	0.001179560806261266	0.0	0.645612947960333	1.769341209391899E-4
88	7.86373870840844E-5	0.0012385388465743293	0.0	0.6456326073071039	1.769341209391899E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8380	0.0	52.119213	1
GTATCAA	15505	0.0	38.79403	1
CTTATAC	9395	0.0	34.34527	1
ATCAACG	18765	0.0	31.596067	3
TCAACGC	18785	0.0	31.585083	4
TATCAAC	19210	0.0	31.178932	2
CAACGCA	19230	0.0	30.832045	5
AACGCAG	19780	0.0	30.039286	6
GTGGTAT	3670	0.0	28.418068	1
TATACAC	12310	0.0	27.833532	3
TGGTATC	3615	0.0	26.966318	2
ACGCAGA	22315	0.0	26.550508	7
TTATACA	12710	0.0	26.291698	2
CGCAGAG	22655	0.0	26.152044	8
GCAGAGT	25955	0.0	22.794193	9
TACACAT	15265	0.0	22.780106	5
ACACATC	14950	0.0	21.978931	6
ACATCTC	15355	0.0	21.454659	8
GAGTACT	14180	0.0	21.31143	12-13
CACATCT	15865	0.0	20.550346	7
>>END_MODULE