FastQCFastQC Report
Fri 27 May 2016
SRR2140154_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140154_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23949874
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA296700.050.6371351
GTATCAA553250.038.114051
TCAACGC652550.031.6704884
ATCAACG654100.031.5490063
CAACGCA671350.030.7448335
TATCAAC682300.030.5235462
AACGCAG694100.029.762146
ACGCAGA803600.025.6850977
CGCAGAG811600.025.4105328
GCAGAGT956850.021.5123989
TGGTATC140300.020.5076312
GTGGTAT151750.020.5045431
TACATGG685400.020.1029242
GAGTACT539200.020.01307712-13
ATCTCGT478500.019.8995494
GTACATG710900.019.7725221
ACATGGG698550.019.1373673
CAGAGTA931350.019.00794610-11
AGAGTAC850050.018.87091410-11
GTACTTT607550.017.7081514-15
Produced by FastQC (version 0.11.5)