##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140154_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23949874
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.493086393690422	32.0	28.0	33.0	18.0	34.0
2	31.125817071104425	33.0	30.0	33.0	27.0	34.0
3	32.36331886338943	33.0	33.0	33.0	30.0	34.0
4	32.46818718127703	33.0	33.0	34.0	31.0	34.0
5	32.944648852849916	33.0	33.0	34.0	32.0	34.0
6	36.77144455958307	38.0	37.0	38.0	34.0	38.0
7	36.98149710516223	38.0	38.0	38.0	35.0	38.0
8	37.168900178765035	38.0	38.0	38.0	36.0	38.0
9	37.20691303010613	38.0	38.0	38.0	36.0	38.0
10-11	37.287010528740154	38.0	38.0	38.0	36.5	38.0
12-13	37.33235026622687	38.0	38.0	38.0	37.0	38.0
14-15	37.32983455361811	38.0	38.0	38.0	37.0	38.0
16-17	37.37423760559241	38.0	38.0	38.0	37.0	38.0
18-19	37.375653771706695	38.0	38.0	38.0	37.0	38.0
20-21	37.39308305808079	38.0	38.0	38.0	37.0	38.0
22-23	37.329385960873516	38.0	38.0	38.0	37.0	38.0
24-25	37.39802101857239	38.0	38.0	38.0	37.0	38.0
26-27	37.369575653155735	38.0	38.0	38.0	37.0	38.0
28-29	37.344169671536804	38.0	38.0	38.0	37.0	38.0
30-31	37.355325781316914	38.0	38.0	38.0	37.0	38.0
32-33	37.336588502643465	38.0	38.0	38.0	37.0	38.0
34-35	37.24804441418621	38.0	38.0	38.0	37.0	38.0
36-37	37.27161372676996	38.0	38.0	38.0	37.0	38.0
38-39	37.26191444743033	38.0	38.0	38.0	37.0	38.0
40-41	37.28435968593467	38.0	38.0	38.0	37.0	38.0
42-43	37.26097315546249	38.0	38.0	38.0	37.0	38.0
44-45	37.22666069098547	38.0	38.0	38.0	37.0	38.0
46-47	37.19494649256086	38.0	38.0	38.0	37.0	38.0
48-49	37.138496596176594	38.0	38.0	38.0	36.5	38.0
50-51	37.10197620162112	38.0	38.0	38.0	36.0	38.0
52-53	37.01241763000557	38.0	38.0	38.0	36.0	38.0
54-55	37.05698864809466	38.0	38.0	38.0	36.0	38.0
56-57	37.02689353368754	38.0	38.0	38.0	36.0	38.0
58-59	37.0105519165471	38.0	38.0	38.0	36.0	38.0
60-61	37.00480713916306	38.0	38.0	38.0	36.0	38.0
62-63	37.01704036812675	38.0	38.0	38.0	36.0	38.0
64-65	37.02804654861428	38.0	38.0	38.0	36.0	38.0
66-67	37.02639830155568	38.0	38.0	38.0	36.0	38.0
68-69	37.011842114636664	38.0	38.0	38.0	36.0	38.0
70-71	37.015372173089105	38.0	38.0	38.0	36.0	38.0
72-73	37.029675551601144	38.0	38.0	38.0	36.0	38.0
74-75	36.9714529867024	38.0	38.0	38.0	36.0	38.0
76-77	36.97591137304212	38.0	38.0	38.0	36.0	38.0
78-79	36.98108670131526	38.0	38.0	38.0	36.0	38.0
80-81	36.96180216427196	38.0	38.0	38.0	36.0	38.0
82-83	36.89779662842011	38.0	38.0	38.0	35.0	38.0
84-85	36.91653707839647	38.0	38.0	38.0	35.0	38.0
86-87	36.915839028042214	38.0	38.0	38.0	35.0	38.0
88-89	36.89786409817022	38.0	38.0	38.0	35.0	38.0
90-91	36.91525559289853	38.0	38.0	38.0	35.0	38.0
92-93	36.89425457813229	38.0	38.0	38.0	35.0	38.0
94-95	36.79995240747326	38.0	38.0	38.0	35.0	38.0
96-97	36.784677611189196	38.0	38.0	38.0	35.0	38.0
98-99	36.83173330923454	38.0	38.0	38.0	35.0	38.0
100	35.36811273820191	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	13.0
19	38.0
20	123.0
21	424.0
22	1442.0
23	4676.0
24	11652.0
25	23724.0
26	42813.0
27	69957.0
28	107279.0
29	154869.0
30	207959.0
31	268146.0
32	344854.0
33	456337.0
34	662313.0
35	1149240.0
36	3185685.0
37	1.7258329E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.808978786276704	20.99714595575743	14.07490494522017	24.118970312745695
2	14.78048764801784	20.463833113758408	42.34010644751799	22.415572790705767
3	18.03518465274598	26.498615399813797	30.51717098803944	24.94902895940079
4	12.69717744652853	18.28137383937803	38.68503859352245	30.33641012057099
5	13.457878734560358	37.45152897255326	35.83039727056602	13.260195022320367
6	29.760365336368782	38.20044731759341	18.5837512130544	13.455436132983415
7	26.381579293486052	32.951041830115685	22.765911002287528	17.901467874110736
8	24.162039432858812	37.1930140425791	21.14546406381929	17.499482460742797
9	25.508706225343815	17.189522583709625	20.942723957545663	36.359047233400894
10-11	24.141392142605845	26.917438062513398	28.90753412731942	20.03363566756134
12-13	25.147925621654625	24.257912588600675	28.93553009923977	21.658631690504926
14-15	22.275037856149055	25.719406707525895	26.422696420031272	25.58285901629378
16-17	21.8864637868241	29.01683741634716	27.057008734158682	22.039690062670058
18-19	21.969226226409376	27.87388359537925	28.834400130873338	21.322490047338036
20-21	22.711635543551097	27.059498574006362	28.673023400458696	21.555842481983845
22-23	22.345421086342984	27.20063965236263	28.58155560316208	21.87238365813231
24-25	22.261340789844848	27.27358143689432	28.725916200686896	21.739161572573934
26-27	22.172723633053476	27.445619086691618	28.709149699323895	21.672507580931008
28-29	22.108700064728456	27.277777071036425	28.811275598023816	21.8022472662113
30-31	22.223496214101054	26.964914159117164	28.92540576136065	21.88618386542113
32-33	21.913813805548255	27.458154357246844	28.732542434671345	21.895489402533553
34-35	22.229796024947003	27.289143375753373	28.742871824022448	21.73818877527718
36-37	22.10635266142139	27.245093920940793	28.946131992500383	21.70242142513743
38-39	21.916600602183074	27.249234871501283	28.905426651579997	21.928737874735646
40-41	22.27790158172105	27.1013708047877	28.94354244732285	21.6771851661684
42-43	22.300127741255654	27.084441133022153	28.755075583681833	21.86035554204036
44-45	22.057524101221848	27.40610073020917	28.612685679941908	21.923689488627073
46-47	22.421630426770456	27.090528155865456	28.35130807180623	22.136533345557858
48-49	22.214072306601796	27.330386964888376	28.330017888436338	22.125522840073494
50-51	21.97141396399485	27.53037302151239	28.29270405928237	22.20550895521039
52-53	22.10681018503193	27.390045680670898	28.359640118040772	22.143504016256394
54-55	22.198825614111207	27.15343995375521	28.438501971352203	22.20923246078138
56-57	22.280905662866672	27.137648576217334	28.230157011064165	22.351288749851825
58-59	21.797237085535134	27.21670821008785	28.613486656253116	22.3725680481239
60-61	22.136492467404477	27.740735868451218	28.432474157959692	21.69029750618462
62-63	21.509457085968194	28.188085098369996	28.371747767567378	21.930710048094433
64-65	21.321282158419606	28.502819614415024	28.08654874292672	22.08934948423865
66-67	21.51366869998918	29.043787017989654	27.578870468410056	21.86367381361111
68-69	21.67769324523792	28.803243094561072	27.5180279556935	22.001035704507512
70-71	21.725512568470993	27.94471039601619	27.63345668795601	22.69632034755681
72-73	22.03513377268378	27.374803733637698	27.63515674500831	22.954905748670214
74-75	21.97420037461681	27.459374952333732	27.829616627657828	22.736808045391626
76-77	22.337585253343264	27.2620223218623	27.275344730595297	23.12504769419914
78-79	22.576746038931997	27.35677891849482	26.812189997191137	23.254285045382044
80-81	22.443548470438518	27.62277361860148	26.909155420650936	23.024522490309064
82-83	22.35786417312436	27.38447406969149	27.05510971291988	23.202552044264273
84-85	22.509936562915858	27.724406216833746	26.968427286268838	22.797229933981562
86-87	22.835872299047907	27.63351756909297	27.057658403883984	22.472951727975136
88-89	22.63275682936409	27.828596083332595	27.325187112366063	22.21345997493725
90-91	22.661837127002148	27.410085027112842	27.219255214870003	22.708822631015007
92-93	22.649522199334882	27.007998344061697	27.37502285213158	22.967456604471845
94-95	21.945918550145674	27.522163828882878	27.909168224144437	22.62274939682701
96-97	22.042530198915706	27.34253676637807	28.189503133076926	22.425429901629297
98-99	23.237849664452824	29.24418052502166	28.47277942914052	19.045190381384995
100	22.31849390660478	30.317311800294856	22.14738420461696	25.216810088483406
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5236.0
1	4488.0
2	4811.0
3	6449.5
4	8445.0
5	11850.5
6	14867.0
7	18759.0
8	23760.5
9	26830.0
10	27132.5
11	27491.5
12	30840.5
13	38554.5
14	53193.0
15	69584.5
16	82106.5
17	89485.0
18	89290.0
19	83008.5
20	75052.5
21	68948.5
22	69289.5
23	78857.0
24	98150.5
25	127757.5
26	169375.5
27	217641.0
28	262110.0
29	310901.5
30	359215.0
31	405007.0
32	459315.5
33	516167.5
34	573974.0
35	628694.5
36	691255.0
37	748227.0
38	781858.5
39	814328.5
40	863277.0
41	905935.5
42	932117.5
43	959912.0
44	1002471.5
45	1072449.5
46	1093214.5
47	1068866.0
48	1048845.0
49	1016232.0
50	985426.5
51	949717.0
52	902801.0
53	851445.0
54	795954.0
55	732380.5
56	664384.5
57	594164.0
58	523801.0
59	454041.5
60	377872.5
61	300764.5
62	231962.0
63	175452.5
64	129996.0
65	93924.0
66	68738.0
67	52450.5
68	39899.5
69	28798.5
70	20854.5
71	15112.5
72	10724.0
73	7304.0
74	4872.5
75	3212.5
76	2072.0
77	1180.0
78	706.5
79	472.0
80	323.5
81	212.0
82	143.0
83	101.0
84	70.0
85	45.5
86	30.0
87	22.5
88	14.5
89	9.0
90	7.5
91	6.5
92	5.5
93	4.0
94	2.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.018225565612579004
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	4.551725220783729E-4
26-27	3.716922133717944E-4
28-29	0.0038517660775671744
30-31	0.006104752493368033
32-33	0.004252052631279087
34-35	0.0020452418843951593
36-37	0.0
38-39	4.231963751368406E-6
40-41	4.123592746923444E-4
42-43	2.1381794172007124E-6
44-45	6.459302946475632E-6
46-47	3.733327869362002E-4
48-49	0.0014178216151106177
50-51	0.0028130644178965044
52-53	0.0
54-55	0.003000779251732143
56-57	0.03973919018350172
58-59	0.06302427553913013
60-61	0.04296716220923769
62-63	0.014071468862286132
64-65	3.6930425502620256E-4
66-67	0.0
68-69	1.8507113081676538E-5
70-71	0.0028444391670798515
72-73	0.005197881623955629
74-75	0.004288883510385648
76-77	0.00313628905738374
78-79	0.007750497126677345
80-81	0.01576235980460972
82-83	0.04290345943867535
84-85	0.019567076427169247
86-87	0.001945115723241641
88-89	0.0013532453624933748
90-91	1.318508608542705E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	137.0
20-21	741.0
22-23	1691.0
24-25	1931.0
26-27	5509.0
28-29	14079.0
30-31	42704.0
32-33	54814.0
34-35	76463.0
36-37	118916.0
38-39	75516.0
40-41	136893.0
42-43	153744.0
44-45	183231.0
46-47	186628.0
48-49	176214.0
50-51	90242.0
52-53	15899.0
54-55	14840.0
56-57	14840.0
58-59	98615.0
60-61	186612.0
62-63	182783.0
64-65	209326.0
66-67	234908.0
68-69	238126.0
70-71	279895.0
72-73	194135.0
74-75	187212.0
76-77	195092.0
78-79	202531.0
80-81	205018.0
82-83	192403.0
84-85	188624.0
86-87	193967.0
88-89	202585.0
90-91	210527.0
92-93	208644.0
94-95	242986.0
96-97	816140.0
98-99	1818022.0
100-101	1.6096691E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.55467449358605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.6224218939041	68.91218577615622
2	8.778122825406557	13.966814080798729
3	2.3682037472788813	5.652050347477864
4	0.9498985130461833	3.022754680293221
5	0.4644760793828331	1.8475621652679157
6	0.2578846283021801	1.2309556596887619
7	0.15433891217002854	0.8594867343566549
8	0.09657968361277615	0.6146692234006347
9	0.06900354167958472	0.49405988675015644
>10	0.23383077349824147	2.8352691149465197
>50	0.0039742375025313146	0.21690952794172097
>100	0.0011242362188602772	0.16252193090309372
>500	6.523314346944579E-5	0.03455842315666795
>1k	7.569485383094381E-5	0.15020244886182488
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.350774622029326E-6
2	0.0	2.505232386608798E-5	0.0	4.175387311014663E-6	8.350774622029326E-6
3	0.0	2.505232386608798E-5	0.0	8.350774622029326E-6	8.350774622029326E-6
4	0.0	6.263080966521995E-5	0.0	1.252616193304399E-5	8.350774622029326E-6
5	8.350774622029326E-6	6.680619697623461E-5	0.0	1.252616193304399E-5	8.350774622029326E-6
6	9.603390815333726E-5	6.680619697623461E-5	0.0	1.252616193304399E-5	1.252616193304399E-5
7	9.603390815333726E-5	6.680619697623461E-5	0.0	1.6701549244058652E-5	1.6701549244058652E-5
8	1.0020929546435192E-4	9.18585208423226E-5	0.0	1.6701549244058652E-5	2.0876936555073316E-5
9	1.0020929546435192E-4	2.1712014017276248E-4	0.0	2.9227711177102644E-5	3.3403098488117305E-5
10-11	1.1899853836391791E-4	2.1712014017276248E-4	0.0	2.9227711177102644E-5	3.3403098488117305E-5
12-13	1.2734931298594723E-4	2.1712014017276248E-4	0.0	4.59292604211613E-5	3.757848579913197E-5
14-15	1.503139431965279E-4	3.4029406584769507E-4	0.0	5.636772869869795E-5	7.724466525377128E-5
16-17	2.1294475286174784E-4	3.465571468142171E-4	0.0	6.054311600971262E-5	9.81216018088446E-5
18-19	2.1294475286174784E-4	3.6534638971378306E-4	0.0	8.350774622029327E-5	1.252616193304399E-4
20-21	2.1294475286174784E-4	3.883110199243637E-4	0.0	9.394621449782992E-5	1.586647178185572E-4
22-23	2.1294475286174784E-4	4.3215258669001763E-4	0.0	1.0647237643087392E-4	1.7327857340710854E-4
24-25	2.1503244651725517E-4	4.864326217332083E-4	0.0	1.3361239395246925E-4	1.8162934802913786E-4
26-27	2.2547091479479183E-4	5.177480265658183E-4	0.0	2.0668167189522583E-4	1.8789242899565985E-4
28-29	2.2547091479479183E-4	5.21923413876833E-4	0.0	6.200450156856775E-4	1.9206781630667452E-4
30-31	2.359093830723285E-4	5.991680791306042E-4	0.0	0.0019540812615548623	1.983308972731965E-4
32-33	2.379970767278358E-4	6.096065474081409E-4	0.0	0.004807958488633385	2.0668167189522583E-4
34-35	2.4217246403885047E-4	6.889389063174195E-4	0.0	0.00881841800086297	2.2547091479479183E-4
36-37	2.463478513498651E-4	7.724466525377128E-4	0.0	0.018467738076617855	2.2964630210580648E-4
38-39	2.505232386608798E-4	8.079374446813374E-4	0.0	0.1679194637934212	2.379970767278358E-4
40-41	2.505232386608798E-4	9.144098211122112E-4	0.0	0.32363635817040204	2.442601576943578E-4
42-43	2.505232386608798E-4	9.457252259448211E-4	0.0	0.3451917951635153	2.5261093231638713E-4
44-45	2.5261093231638713E-4	0.001075162232586276	0.0	0.37329841484761045	2.609617069384165E-4
46-47	2.5469862597189447E-4	0.0011607576724620764	0.0	0.4047891859472831	2.714001752159531E-4
48-49	2.609617069384165E-4	0.001164933059773091	0.0	0.43553673810559507	2.7975094983798246E-4
50-51	2.755755625269678E-4	0.0013027208410365749	0.0	0.4679356559454133	2.8810172446001175E-4
52-53	2.8601403080450447E-4	0.001315247002969619	0.0	0.9349485512950924	3.110663546705924E-4
54-55	2.901894181155191E-4	0.0013256854712471555	0.0	1.5578140411093604	3.4029406584769507E-4
56-57	2.9227711177102643E-4	0.001340299326835707	0.0	2.2379449678941943	3.569956150917537E-4
58-59	2.9227711177102643E-4	0.0013423870204912142	0.0	2.942481033511909	3.674340833692904E-4
60-61	3.089786610150851E-4	0.0013820531999458536	0.0	3.13013129004353	3.71609470680305E-4
62-63	3.465571468142171E-4	0.0014008424428454194	0.0	3.13675762970611	3.71609470680305E-4
64-65	3.465571468142171E-4	0.0015762087099080355	0.0	3.1427806259022493	3.757848579913197E-4
66-67	3.549079214362464E-4	0.0015991733401186162	0.0	3.1482441201987115	3.883110199243637E-4
68-69	3.549079214362464E-4	0.0016430149068842699	0.0	3.1534048989151255	3.945741008908857E-4
70-71	3.6743408336929035E-4	0.0016638918434393433	0.0	3.157993649569931	4.21714118412481E-4
72-73	3.96661794546393E-4	0.00167433031171688	0.0	3.1616492011607242	4.6346799152262763E-4
74-75	4.008371818574077E-4	0.0017119087975160118	0.0	3.16447176298297	4.6346799152262763E-4
76-77	4.1127565013494437E-4	0.0018037673183583347	0.0	3.1673590433085366	4.697310724891496E-4
78-79	4.1962642475697366E-4	0.0018413458041574666	0.0	3.169206652193661	4.759941534556716E-4
80-81	4.21714118412481E-4	0.0018434334978129738	0.0	3.1698141710474133	4.885203153887156E-4
82-83	4.258895057234957E-4	0.0018476088851239886	0.0	3.170302691362802	5.093972519437889E-4
84-85	4.27977199379003E-4	0.001858047353401525	0.0	3.1705177238093194	5.093972519437889E-4
86-87	4.363279740010323E-4	0.0018998012265116718	0.0	3.170653423896927	5.135726392548036E-4
88	4.425910549675543E-4	0.0019958351346650092	0.0	3.170668037752516	5.344495758098769E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	29670	0.0	50.637135	1
GTATCAA	55325	0.0	38.11405	1
TCAACGC	65255	0.0	31.670488	4
ATCAACG	65410	0.0	31.549006	3
CAACGCA	67135	0.0	30.744833	5
TATCAAC	68230	0.0	30.523546	2
AACGCAG	69410	0.0	29.76214	6
ACGCAGA	80360	0.0	25.685097	7
CGCAGAG	81160	0.0	25.410532	8
GCAGAGT	95685	0.0	21.512398	9
TGGTATC	14030	0.0	20.507631	2
GTGGTAT	15175	0.0	20.504543	1
TACATGG	68540	0.0	20.102924	2
GAGTACT	53920	0.0	20.013077	12-13
ATCTCGT	47850	0.0	19.89954	94
GTACATG	71090	0.0	19.772522	1
ACATGGG	69855	0.0	19.137367	3
CAGAGTA	93135	0.0	19.007946	10-11
AGAGTAC	85005	0.0	18.870914	10-11
GTACTTT	60755	0.0	17.70815	14-15
>>END_MODULE