FastQCFastQC Report
Fri 27 May 2016
SRR2140153_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140153_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6888219
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94700.049.5932661
GTATCAA180650.037.5731241
ATCAACG226350.030.8287373
TCAACGC228450.030.6755644
CAACGCA232300.030.1488745
TATCAAC236100.029.7568682
AACGCAG239300.029.3379946
ACGCAGA269550.026.0140347
CGCAGAG276650.025.3617658
TGGTATC41350.024.9770472
GTGGTAT43700.024.411961
CTTATAC77550.023.0185431
GCAGAGT317750.022.1080469
TATACAC102900.021.0628873
TACATGG226800.020.3702742
GAGTACT190050.019.86793112-13
ACATGGG228000.019.6830393
GTACATG229950.019.57371
AGAGTAC291850.019.5486910-11
CAGAGTA310000.019.19240610-11
Produced by FastQC (version 0.11.5)