##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140153_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6888219
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	21.487690069087524	18.0	18.0	25.0	18.0	33.0
2	28.090473459104597	29.0	27.0	31.0	25.0	33.0
3	30.054713126861966	31.0	29.0	33.0	25.0	33.0
4	31.234237906779676	33.0	31.0	33.0	29.0	33.0
5	32.24826533535011	33.0	33.0	33.0	31.0	33.0
6	35.87766750737745	38.0	36.0	38.0	31.0	38.0
7	36.017683380856504	38.0	36.0	38.0	33.0	38.0
8	35.82666012796631	38.0	36.0	38.0	31.0	38.0
9	36.74023299781845	38.0	37.0	38.0	34.0	38.0
10-11	36.88998389859556	38.0	38.0	38.0	35.0	38.0
12-13	37.06081347007115	38.0	38.0	38.0	36.0	38.0
14-15	36.96722324014379	38.0	38.0	38.0	35.5	38.0
16-17	37.0663021892887	38.0	38.0	38.0	35.5	38.0
18-19	37.20511767991117	38.0	38.0	38.0	36.0	38.0
20-21	37.268039578058406	38.0	38.0	38.0	36.0	38.0
22-23	37.2509422846765	38.0	38.0	38.0	36.5	38.0
24-25	37.32625178546388	38.0	38.0	38.0	37.0	38.0
26-27	37.311812374797164	38.0	38.0	38.0	37.0	38.0
28-29	37.293254872377034	38.0	38.0	38.0	37.0	38.0
30-31	37.29825513040983	38.0	38.0	38.0	37.0	38.0
32-33	37.28042055296977	38.0	38.0	38.0	37.0	38.0
34-35	37.19440236928635	38.0	38.0	38.0	36.0	38.0
36-37	37.21963223221295	38.0	38.0	38.0	36.0	38.0
38-39	37.200638660574384	38.0	38.0	38.0	36.0	38.0
40-41	37.20612658554894	38.0	38.0	38.0	36.0	38.0
42-43	37.181942784929916	38.0	38.0	38.0	36.0	38.0
44-45	37.140990891200225	38.0	38.0	38.0	36.0	38.0
46-47	37.08847528692243	38.0	38.0	38.0	36.0	38.0
48-49	37.02505718584085	38.0	38.0	38.0	36.0	38.0
50-51	36.97192303817696	38.0	38.0	38.0	36.0	38.0
52-53	36.86536383236996	38.0	38.0	38.0	35.5	38.0
54-55	36.891222873095366	38.0	38.0	38.0	35.5	38.0
56-57	36.84180961819813	38.0	38.0	38.0	35.0	38.0
58-59	36.81593252664884	38.0	38.0	38.0	35.0	38.0
60-61	36.80770140506483	38.0	38.0	38.0	35.0	38.0
62-63	36.812208137789284	38.0	38.0	38.0	35.0	38.0
64-65	36.8153868037545	38.0	38.0	38.0	35.0	38.0
66-67	36.80660414677749	38.0	38.0	38.0	35.0	38.0
68-69	36.78512679839561	38.0	38.0	38.0	35.0	38.0
70-71	36.78106155345803	38.0	38.0	38.0	35.0	38.0
72-73	36.788692708064445	38.0	38.0	38.0	35.0	38.0
74-75	36.72337385851097	38.0	38.0	38.0	34.5	38.0
76-77	36.71846138430904	38.0	38.0	38.0	34.5	38.0
78-79	36.71436538962971	38.0	38.0	38.0	34.5	38.0
80-81	36.690045160545296	38.0	38.0	38.0	34.0	38.0
82-83	36.62723088427851	38.0	38.0	38.0	34.0	38.0
84-85	36.61465775130138	38.0	38.0	38.0	34.0	38.0
86-87	36.603766282763615	38.0	38.0	38.0	34.0	38.0
88-89	36.57730829056898	38.0	38.0	38.0	34.0	38.0
90-91	36.5815138069903	38.0	38.0	38.0	34.0	38.0
92-93	36.548554426273824	38.0	38.0	38.0	34.0	38.0
94-95	36.474693807702586	38.0	38.0	38.0	34.0	38.0
96-97	36.48647294420051	38.0	38.0	38.0	34.0	38.0
98-99	36.51059711194157	38.0	37.5	38.0	34.0	38.0
100	34.84944194219152	38.0	34.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	5.0
19	15.0
20	42.0
21	156.0
22	565.0
23	1545.0
24	3827.0
25	7562.0
26	13685.0
27	22958.0
28	36166.0
29	52903.0
30	71169.0
31	93729.0
32	124456.0
33	175046.0
34	278407.0
35	533498.0
36	1660922.0
37	3811560.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.90175109124724	17.074340987125993	19.456451660436464	23.567456261190305
2	14.272399735044797	21.093413551686158	41.85336802957137	22.780818683697674
3	18.018779600358233	25.659724233506516	31.60017124891064	24.721324917224614
4	12.390590368860224	18.083818182900398	38.796574266875076	30.7290171813643
5	13.318740301375435	37.03922595956952	35.77294798553879	13.869085753516257
6	29.52925857903182	37.99403009689442	18.779774568723788	13.696936755349967
7	26.136189920790848	32.76963174370617	23.028536113616596	18.06564222188638
8	24.24995488674213	36.65008327987249	21.269213420769578	17.8307484126158
9	25.438491430077935	16.928047729028357	21.12943853846691	36.5040223024268
10-11	24.086160733275175	26.721624268914795	28.931288334473688	20.260926663336342
12-13	25.09324979359687	24.20983566289051	29.014923886711504	21.681990656801126
14-15	22.190503815282295	25.533624874586597	26.785007851811915	25.490863458319197
16-17	21.63361095226502	28.731251140534297	27.4107719281283	22.224365979072385
18-19	21.685235327157862	27.597068269751585	29.40508279426075	21.3126136088298
20-21	22.566152727172422	26.748636150654768	29.29041362081812	21.394797501354684
22-23	22.190757735811363	26.820078178400298	29.175643845178662	21.813520240609677
24-25	22.06140575993256	26.901846888734323	29.368014437618424	21.6687329137147
26-27	21.96756921906732	27.0759984296702	29.31383456341775	21.64259778784473
28-29	21.901577082587156	27.0480308421724	29.367629272752854	21.68276280248759
30-31	22.04185125458219	27.038431563940946	29.370081786086317	21.549635395390553
32-33	21.966170266701283	27.179772547247428	29.194217084553785	21.659840101497508
34-35	22.176994988617935	27.024470344524843	29.2236390986585	21.574895568198723
36-37	21.927318629241004	27.081206324959396	29.243092868113717	21.748382177685883
38-39	21.70484334890347	27.012116285588494	29.424268917156184	21.858771448351856
40-41	22.000307561186077	26.85870503968928	29.364430229537025	21.77655716958762
42-43	22.08215070679547	26.853765696662514	29.18028677226705	21.883796824274963
44-45	21.93270677518731	27.161952563955367	29.01918427505396	21.88615638580336
46-47	22.190214131642115	26.95000036497638	28.60455235038706	22.25523315299445
48-49	22.065627875541498	27.100008532627477	28.62816221322852	22.206201378602504
50-51	21.92151487048178	27.26045677173833	28.653227925065885	22.164800432714017
52-53	22.089812042337716	27.26594478084911	28.611170187221724	22.03307298959145
54-55	22.07890054699022	27.20451289313891	28.46420986278828	22.25237669708259
56-57	22.217303544781128	27.350344180297125	28.243663450664506	22.188688824257238
58-59	21.896793760001536	27.5036013504665	28.6139192594793	21.98568563005267
60-61	22.087006449545132	27.7209164490378	28.297767824428067	21.894309276989
62-63	21.835575509105006	27.890972675444374	28.30542378108718	21.968028034363435
64-65	21.786835293957655	27.81199273894223	28.35753877078137	22.043633196318744
66-67	21.87309843843785	27.821137495612497	28.20939737347236	22.096366692477293
68-69	21.966414470454033	27.672613773101702	28.32012296259146	22.040848793852806
70-71	21.94036881416706	27.241099211647363	28.419557428415903	22.39897454576968
72-73	22.08079277364342	27.14530641399684	28.486483889935286	22.287416922424452
74-75	21.980905697819093	27.41105048398782	28.328101877540973	22.279941940652115
76-77	22.192352170959378	27.262903691938916	28.18283997705091	22.361904160050795
78-79	22.20149016807956	27.356643107496414	28.123086317149987	22.31878040727404
80-81	22.183062809807737	27.38539030337001	28.033200906237767	22.39834598058449
82-83	22.303822427252975	27.331423094379854	28.09511919499999	22.26963528336718
84-85	22.427162106324047	27.35335672294107	27.98406992823606	22.235411242498827
86-87	22.22347934364659	27.38514127818551	28.08859109306085	22.30278828510705
88-89	22.313325044183202	27.438836551138806	28.00632499810484	22.24151340657315
90-91	22.40526003419338	27.394671022844662	28.020823066973417	22.179245875988542
92-93	22.393432358516176	27.460727132550346	27.969976148495196	22.175864360438283
94-95	22.230302207256138	27.604734014180803	27.920244552646356	22.2447192259167
96-97	22.36244093995432	27.608469054100564	28.0936707969586	21.935419208986513
98-99	23.35660191570293	29.583699963861648	28.270930745058493	18.788767375376928
100	29.76665705298397	40.56363134851217	29.669711598503863	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1958.0
1	1833.0
2	2143.5
3	2736.5
4	3407.0
5	4651.0
6	5786.0
7	8014.0
8	10365.5
9	10909.5
10	10417.0
11	10084.0
12	11104.5
13	13800.0
14	19470.5
15	26284.0
16	30940.0
17	32728.5
18	31579.0
19	28616.5
20	25387.0
21	22956.0
22	22856.0
23	26020.0
24	32093.0
25	40998.5
26	54137.0
27	68721.0
28	81519.5
29	96052.0
30	110685.5
31	124114.5
32	140528.0
33	159276.0
34	176376.0
35	193030.5
36	211378.5
37	227027.0
38	237813.0
39	246337.0
40	257677.5
41	268183.5
42	275555.0
43	287351.0
44	302530.0
45	308359.5
46	303490.5
47	298648.5
48	293769.0
49	287720.5
50	280996.5
51	271563.5
52	259225.5
53	245131.0
54	228970.5
55	210744.5
56	191707.0
57	171947.5
58	152290.5
59	131135.0
60	109460.0
61	88315.5
62	68519.0
63	52531.5
64	39558.0
65	29269.5
66	21454.5
67	16200.5
68	12252.0
69	8888.5
70	6448.5
71	4661.0
72	3315.0
73	2267.0
74	1569.5
75	1078.0
76	710.0
77	450.0
78	272.0
79	159.5
80	99.5
81	67.0
82	45.5
83	30.5
84	19.0
85	16.5
86	14.5
87	9.0
88	4.5
89	1.5
90	1.5
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.01476433893870099
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	3.9202519124840064E-4
26-27	3.194668504859563E-4
28-29	0.003951212117844611
30-31	0.00634016835473649
32-33	0.004320685504078275
34-35	0.0017681416816313312
36-37	0.0
38-39	0.0
40-41	5.197771878554209E-4
42-43	0.0
44-45	1.5067286735740132E-5
46-47	4.4101118085001714E-4
48-49	0.0014885837138813192
50-51	0.0030255018077179853
52-53	0.0
54-55	0.00273307000570332
56-57	0.039011326818834846
58-59	0.06096218421305274
60-61	0.04188154469568989
62-63	0.013737899869709172
64-65	3.4407818504983564E-4
66-67	0.0
68-69	0.0
70-71	0.002809662938616312
72-73	0.005060241706597767
74-75	0.004140503267367077
76-77	0.003220729417531521
78-79	0.007980390637035343
80-81	0.01572190831881463
82-83	0.04141470394926762
84-85	0.019068626768658347
86-87	0.001902496065647327
88-89	0.0012262638971930076
90-91	1.221391947118614E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	52.0
20-21	220.0
22-23	517.0
24-25	671.0
26-27	1825.0
28-29	4942.0
30-31	13758.0
32-33	17605.0
34-35	23862.0
36-37	37680.0
38-39	42765.0
40-41	43098.0
42-43	49628.0
44-45	60372.0
46-47	60692.0
48-49	55820.0
50-51	49736.0
52-53	46365.0
54-55	49308.0
56-57	50472.0
58-59	53687.0
60-61	53504.0
62-63	54247.0
64-65	59343.0
66-67	64250.0
68-69	65403.0
70-71	74306.0
72-73	56085.0
74-75	55271.0
76-77	57604.0
78-79	59931.0
80-81	59927.0
82-83	55630.0
84-85	55351.0
86-87	57256.0
88-89	59941.0
90-91	61241.0
92-93	60855.0
94-95	70602.0
96-97	213809.0
98-99	1591597.0
100-101	3338991.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.1843240100427
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.89539717153092	79.2465375802427
2	6.551344232320409	11.42349036503894
3	1.4854048532438027	3.885120540338928
4	0.5131258199213605	1.7894611096777087
5	0.23180032279282162	1.0104677224000924
6	0.1099195177168178	0.5749955308590402
7	0.06728914399780224	0.41065909728639677
8	0.040942418948953174	0.28556296955203564
9	0.025893561108752912	0.20317613593314188
>10	0.07756331661833703	0.9966622078329246
>50	9.414722720419372E-4	0.05359688140618009
>100	3.055104791506846E-4	0.04851852189206078
>500	2.421973663497307E-5	0.01239498261971958
>1k	4.8439312270969406E-5	0.059356354920100496
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.4517540746018673E-5	0.0	0.0	0.0
3	0.0	1.4517540746018673E-5	0.0	1.4517540746018673E-5	0.0
4	0.0	2.9035081492037347E-5	0.0	1.4517540746018673E-5	0.0
5	0.0	2.9035081492037347E-5	0.0	1.4517540746018673E-5	0.0
6	5.8070162984074694E-5	2.9035081492037347E-5	0.0	1.4517540746018673E-5	0.0
7	5.8070162984074694E-5	2.9035081492037347E-5	0.0	1.4517540746018673E-5	0.0
8	5.8070162984074694E-5	2.9035081492037347E-5	0.0	1.4517540746018673E-5	0.0
9	5.8070162984074694E-5	8.710524447611204E-5	0.0	7.258770373009337E-5	1.4517540746018673E-5
10-11	5.8070162984074694E-5	8.710524447611204E-5	0.0	8.710524447611204E-5	1.4517540746018673E-5
12-13	5.8070162984074694E-5	8.710524447611204E-5	0.0	1.0162278522213071E-4	1.4517540746018673E-5
14-15	5.8070162984074694E-5	1.596929482062054E-4	0.0	1.0888155559514005E-4	1.4517540746018673E-5
16-17	5.8070162984074694E-5	1.742104889522241E-4	0.0	1.1614032596814939E-4	1.4517540746018673E-5
18-19	7.258770373009337E-5	2.1050434081727076E-4	0.0	1.233990963411587E-4	1.4517540746018673E-5
20-21	7.258770373009337E-5	2.467981926823174E-4	0.0	1.3065786671416805E-4	1.4517540746018673E-5
22-23	7.98464741031027E-5	2.613157334283361E-4	0.0	1.3065786671416805E-4	1.4517540746018673E-5
24-25	8.710524447611204E-5	3.0486835566639214E-4	0.0	1.6695171857921475E-4	1.4517540746018673E-5
26-27	8.710524447611204E-5	3.193858964124108E-4	0.0	2.758332741743548E-4	1.4517540746018673E-5
28-29	8.710524447611204E-5	3.193858964124108E-4	0.0	6.387717928248216E-4	1.4517540746018673E-5
30-31	8.710524447611204E-5	3.484209779044482E-4	0.0	0.0022066661933948383	1.4517540746018673E-5
32-33	8.710524447611204E-5	3.484209779044482E-4	0.0	0.005872345231764553	1.4517540746018673E-5
34-35	8.710524447611204E-5	3.992323705155135E-4	0.0	0.010191313603705109	1.4517540746018673E-5
36-37	8.710524447611204E-5	4.5004376312657887E-4	0.0	0.017638812006412687	1.4517540746018673E-5
38-39	8.710524447611204E-5	4.5004376312657887E-4	0.0	0.03643902727250687	1.4517540746018673E-5
40-41	8.710524447611204E-5	4.790788446186162E-4	0.0	0.0571410403763295	1.4517540746018673E-5
42-43	8.710524447611204E-5	5.081139261106536E-4	0.0	0.06931399829186616	1.4517540746018673E-5
44-45	8.710524447611204E-5	5.879604002137562E-4	0.0	0.08375895133415473	1.4517540746018673E-5
46-47	8.710524447611204E-5	6.242542520788029E-4	0.0	0.09994600926596556	1.4517540746018673E-5
48-49	8.710524447611204E-5	6.242542520788029E-4	0.0	0.11518942704928517	1.4517540746018673E-5
50-51	1.0162278522213071E-4	7.549121187929711E-4	0.0	0.13074497195864418	1.4517540746018673E-5
52-53	1.0162278522213071E-4	7.694296595389897E-4	0.0	0.17163362546980576	2.177631111902801E-5
54-55	1.0162278522213071E-4	7.839472002850083E-4	0.0	0.22380240814062385	2.9035081492037347E-5
56-57	1.0162278522213071E-4	7.98464741031027E-4	0.0	0.291541253261547	2.9035081492037347E-5
58-59	1.0162278522213071E-4	8.129822817770457E-4	0.0	0.33775058545612446	2.9035081492037347E-5
60-61	1.1614032596814937E-4	8.492761336420923E-4	0.0	0.3594034974788113	2.9035081492037347E-5
62-63	1.742104889522241E-4	8.710524447611204E-4	0.0	0.37672292358881154	4.355262223805602E-5
64-65	1.742104889522241E-4	0.0010452629337133444	0.0	0.383284852006012	4.355262223805602E-5
66-67	1.742104889522241E-4	0.0010452629337133444	0.0	0.3898758155047045	4.355262223805602E-5
68-69	1.742104889522241E-4	0.0010670392448323726	0.0	0.39607480540325446	4.355262223805602E-5
70-71	1.9598680007125208E-4	0.0010742980152053819	0.0	0.40148258933114644	4.355262223805602E-5
72-73	2.613157334283361E-4	0.0010742980152053819	0.0	0.4057434875401029	4.355262223805602E-5
74-75	2.613157334283361E-4	0.0010888155559514005	0.0	0.4092857674821314	4.355262223805602E-5
76-77	2.613157334283361E-4	0.0011323681781894566	0.0	0.41273368340931094	4.355262223805602E-5
78-79	2.613157334283361E-4	0.0011614032596814938	0.0	0.4150637486990469	4.355262223805602E-5
80-81	2.613157334283361E-4	0.0011614032596814938	0.0	0.4157896257363478	4.355262223805602E-5
82-83	2.613157334283361E-4	0.0011686620300545032	0.0	0.41652276154402174	4.355262223805602E-5
84-85	2.685745038013454E-4	0.0011759208004275125	0.0	0.4168494062108072	4.355262223805602E-5
86-87	2.8309204454736415E-4	0.00120495588191955	0.0	0.4170236166997594	4.355262223805602E-5
88	2.9035081492037346E-4	0.0012775435856496433	0.0	0.41704539301087845	4.355262223805602E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9470	0.0	49.593266	1
GTATCAA	18065	0.0	37.573124	1
ATCAACG	22635	0.0	30.828737	3
TCAACGC	22845	0.0	30.675564	4
CAACGCA	23230	0.0	30.148874	5
TATCAAC	23610	0.0	29.756868	2
AACGCAG	23930	0.0	29.337994	6
ACGCAGA	26955	0.0	26.014034	7
CGCAGAG	27665	0.0	25.361765	8
TGGTATC	4135	0.0	24.977047	2
GTGGTAT	4370	0.0	24.41196	1
CTTATAC	7755	0.0	23.018543	1
GCAGAGT	31775	0.0	22.108046	9
TATACAC	10290	0.0	21.062887	3
TACATGG	22680	0.0	20.370274	2
GAGTACT	19005	0.0	19.867931	12-13
ACATGGG	22800	0.0	19.683039	3
GTACATG	22995	0.0	19.5737	1
AGAGTAC	29185	0.0	19.54869	10-11
CAGAGTA	31000	0.0	19.192406	10-11
>>END_MODULE