FastQCFastQC Report
Fri 27 May 2016
SRR2140152_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140152_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5689410
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73200.048.890471
GTATCAA140250.037.0829281
CTTATAC72050.033.2118451
ATCAACG171550.030.780933
TCAACGC171800.030.7114934
CAACGCA174800.030.208635
TATCAAC178100.029.796532
AACGCAG181350.029.0942066
TATACAC96800.027.0345523
ACGCAGA205550.025.648267
CGCAGAG207900.025.3583438
TTATACA104550.023.9004752
GCAGAGT239950.021.9006529
TACACAT120950.021.671595
ACACATC114200.021.283936
GTGGTAT35900.021.2352141
ACATCTC117800.020.7053818
TGGTATC35750.020.6134992
GAGTACT146850.019.98321312-13
ATACACA146700.019.4552064
Produced by FastQC (version 0.11.5)