##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140152_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5689410
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	21.394153348062453	18.0	18.0	25.0	18.0	33.0
2	28.072193426031873	29.0	27.0	31.0	25.0	33.0
3	30.09121051216207	31.0	29.0	33.0	27.0	33.0
4	31.253084766258716	33.0	31.0	33.0	29.0	33.0
5	32.26536811374115	33.0	33.0	33.0	31.0	33.0
6	35.915306332291046	38.0	36.0	38.0	31.0	38.0
7	36.053564077821775	38.0	36.0	38.0	33.0	38.0
8	35.883067488544505	38.0	36.0	38.0	31.0	38.0
9	36.7722516042964	38.0	37.0	38.0	34.0	38.0
10-11	36.91321384818461	38.0	38.0	38.0	35.0	38.0
12-13	37.085039573523446	38.0	38.0	38.0	36.0	38.0
14-15	36.98972775735973	38.0	38.0	38.0	35.5	38.0
16-17	37.088026702241535	38.0	38.0	38.0	36.0	38.0
18-19	37.218306203982486	38.0	38.0	38.0	36.0	38.0
20-21	37.28067450917986	38.0	38.0	38.0	36.5	38.0
22-23	37.263743905376685	38.0	38.0	38.0	36.5	38.0
24-25	37.33484889798048	38.0	38.0	38.0	37.0	38.0
26-27	37.3197272719225	38.0	38.0	38.0	37.0	38.0
28-29	37.301385528518246	38.0	38.0	38.0	37.0	38.0
30-31	37.30660772724407	38.0	38.0	38.0	37.0	38.0
32-33	37.28876578424856	38.0	38.0	38.0	37.0	38.0
34-35	37.20134361650198	38.0	38.0	38.0	36.0	38.0
36-37	37.2231067223879	38.0	38.0	38.0	36.0	38.0
38-39	37.194046277527654	38.0	38.0	38.0	36.0	38.0
40-41	37.21373074249526	38.0	38.0	38.0	36.5	38.0
42-43	37.193929822814354	38.0	38.0	38.0	36.0	38.0
44-45	37.15734209527155	38.0	38.0	38.0	36.0	38.0
46-47	37.10917901981192	38.0	38.0	38.0	36.0	38.0
48-49	37.04733290113731	38.0	38.0	38.0	36.0	38.0
50-51	36.998326363399	38.0	38.0	38.0	36.0	38.0
52-53	36.89745349932419	38.0	38.0	38.0	36.0	38.0
54-55	36.9217260530016	38.0	38.0	38.0	36.0	38.0
56-57	36.8757396906292	38.0	38.0	38.0	35.5	38.0
58-59	36.84773809703431	38.0	38.0	38.0	35.0	38.0
60-61	36.83646835381634	38.0	38.0	38.0	35.0	38.0
62-63	36.84134886177362	38.0	38.0	38.0	35.0	38.0
64-65	36.84462080710939	38.0	38.0	38.0	35.0	38.0
66-67	36.835761135734096	38.0	38.0	38.0	35.0	38.0
68-69	36.812673788234974	38.0	38.0	38.0	35.0	38.0
70-71	36.807450457136035	38.0	38.0	38.0	35.0	38.0
72-73	36.816836965292055	38.0	38.0	38.0	35.0	38.0
74-75	36.75241316536457	38.0	38.0	38.0	35.0	38.0
76-77	36.74982506665085	38.0	38.0	38.0	35.0	38.0
78-79	36.74964001663126	38.0	38.0	38.0	35.0	38.0
80-81	36.72818663884627	38.0	38.0	38.0	35.0	38.0
82-83	36.66751530767688	38.0	38.0	38.0	34.0	38.0
84-85	36.65691824769057	38.0	38.0	38.0	34.0	38.0
86-87	36.64640924800893	38.0	38.0	38.0	34.0	38.0
88-89	36.621868856405584	38.0	38.0	38.0	34.0	38.0
90-91	36.62915302789004	38.0	38.0	38.0	34.0	38.0
92-93	36.59490466578648	38.0	38.0	38.0	34.0	38.0
94-95	36.52268053531863	38.0	38.0	38.0	34.0	38.0
96-97	36.53544979330462	38.0	38.0	38.0	34.0	38.0
98-99	36.55967214329644	38.0	38.0	38.0	34.0	38.0
100	34.92834369850499	38.0	35.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	11.0
19	22.0
20	55.0
21	134.0
22	471.0
23	1332.0
24	3149.0
25	6211.0
26	11205.0
27	18689.0
28	29187.0
29	42251.0
30	56787.0
31	74897.0
32	99236.0
33	140902.0
34	222098.0
35	428411.0
36	1369822.0
37	3184538.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.24333103080988	17.19828593826073	19.73919967096764	23.819183359961755
2	14.129107757149962	21.111339052493964	42.1688687034782	22.590684486877873
3	17.935603164475754	25.646508161654722	31.86637981794246	24.551508855927064
4	12.341771818167437	18.313656424831397	38.89368141863568	30.45089033836549
5	13.3078825396658	36.922176464694935	36.054195426239275	13.71574556939999
6	29.30207174381878	38.132583167674674	18.946235198377337	13.619109890129206
7	25.96184841661965	32.723920406509635	23.181278902381795	18.132952274488918
8	24.014757241963576	36.77966608136872	21.55494154929949	17.650635127368215
9	25.182365131006556	16.996999688895688	21.29649647327227	36.52413870682549
10-11	23.980069989682587	26.68318859073261	29.20643792590093	20.13030349368388
12-13	25.001388544682136	24.077145081827464	29.22718700181565	21.694279371674742
14-15	22.10839085247855	25.48371448006032	26.82153334001241	25.586361327448714
16-17	21.7205914145755	28.807143447211576	27.380062256016004	22.092202882196922
18-19	21.714422057823217	27.642778425179408	29.307239239218124	21.33556027777924
20-21	22.43422780824864	26.82907987788689	29.159281112112694	21.577411201751776
22-23	22.14824646609103	26.957637388192634	29.090401414934647	21.803714730781685
24-25	22.035997965394298	27.17510011080989	29.22040269688093	21.568499226914877
26-27	21.959747708942267	27.416838450212072	29.139494099290157	21.483919741555503
28-29	21.778537038110237	27.3441710847334	29.24733062646042	21.62996125069594
30-31	22.331645330040956	27.03845874515552	29.167217963700224	21.4626779611033
32-33	22.017227919285478	27.206448340500604	29.157472709229243	21.618851030984672
34-35	22.0078366588168	27.024549067485015	29.235353013642634	21.73226126005555
36-37	21.85185333203669	27.244622599491734	29.233351584012407	21.67017248445917
38-39	21.700123712504254	26.97757104536039	29.485123189479694	21.837182052655667
40-41	21.9762258957948	26.839388728567037	29.39326057251429	21.791124803123875
42-43	22.14645919357866	26.81435932225233	29.25580140127414	21.783380082894872
44-45	21.90289491838001	27.184845179183387	29.087891531192867	21.82436837124374
46-47	22.241752045836233	26.862285711181684	28.619122804126263	22.276839438855824
48-49	22.099681488035568	26.97386875106907	28.715654694416287	22.210795066479072
50-51	21.85453125304525	27.18370468484595	28.81599472581905	22.145769336289753
52-53	22.086551805522774	27.156167099362754	28.856388954448903	21.900892140665565
54-55	22.032697872694996	27.08848461466537	28.6587544887784	22.220063023861233
56-57	22.29100251980584	27.375556953049973	28.279101992721117	22.05433853442307
58-59	21.85025389182278	27.629099771238558	28.75126437982623	21.76938195711243
60-61	22.076273978057824	27.94114331420473	28.27900657504003	21.703576132697417
62-63	21.74261854130057	28.227056510558096	28.24941001998522	21.78091492815612
64-65	21.678703007453404	28.12876689589638	28.335108426465673	21.85742167018455
66-67	21.79359912072091	28.12279279635049	28.268877083306325	21.814730999622277
68-69	21.891267866272244	27.868948990642888	28.377296372981853	21.86248677010301
70-71	21.955042946150765	27.3711473791539	28.45426316090471	22.219546513790633
72-73	22.12224435816359	27.218093564533525	28.542504053662398	22.11715802364049
74-75	21.957934393351877	27.42325608874152	28.449539576094562	22.16926994181204
76-77	22.15205401343941	27.332367031339093	28.21508390307849	22.300495052143006
78-79	22.187200749276144	27.447269659153257	28.109403883131794	22.256125708438805
80-81	22.198783804673205	27.401270053818184	28.11286883507373	22.28707730643488
82-83	22.233787615314853	27.416224591511977	28.108411164707555	22.241576628465616
84-85	22.398139652305726	27.37253938996384	28.06685265542683	22.162468302303605
86-87	22.176124758552646	27.450242805681548	28.1391024744705	22.234529961295305
88-89	22.26953242749811	27.527922967984846	28.01499235026134	22.1875522542557
90-91	22.346317340934416	27.498214323941777	28.064439944543334	22.09102839058047
92-93	22.354803929207915	27.48637872337898	27.99412135296936	22.16469599444375
94-95	22.254612465194114	27.61636373627073	27.963815321688095	22.165208476847056
96-97	22.267276693114844	27.678606767781755	28.102771529615012	21.951345009488385
98-99	23.306036243905947	29.624874768205917	28.334308693222948	18.734780294665185
100	29.646236942065922	40.70855303621838	29.645210021715695	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1278.0
1	1231.5
2	1556.5
3	2062.5
4	2620.0
5	3627.0
6	4555.5
7	6055.5
8	7506.0
9	7877.0
10	7632.5
11	7548.5
12	8416.5
13	10388.5
14	14606.5
15	19800.0
16	23438.0
17	24940.0
18	24413.5
19	22547.0
20	20320.0
21	18469.0
22	18504.5
23	21135.0
24	26371.5
25	34408.0
26	45656.5
27	58277.0
28	69469.5
29	81524.0
30	93819.0
31	105680.5
32	119370.0
33	134264.0
34	149318.5
35	162938.0
36	177838.0
37	191267.0
38	199430.5
39	206277.5
40	215694.5
41	224386.5
42	231720.0
43	245915.0
44	262712.5
45	267261.5
46	260153.5
47	252861.0
48	245669.5
49	238169.5
50	231087.0
51	222609.0
52	210827.0
53	197630.0
54	184014.5
55	168343.5
56	152121.0
57	136133.5
58	120399.5
59	103139.0
60	86058.5
61	69859.5
62	54435.5
63	41921.5
64	31824.5
65	23560.5
66	17297.0
67	13190.0
68	9988.5
69	7227.0
70	5224.0
71	3728.5
72	2636.5
73	1805.0
74	1181.0
75	785.5
76	513.5
77	324.0
78	202.0
79	117.5
80	71.0
81	50.0
82	43.5
83	37.0
84	26.5
85	17.5
86	9.5
87	4.5
88	1.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.014289706665541769
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	2.900544036586935E-4
26-27	2.6371818020390694E-4
28-29	0.0035175036698555477
30-31	0.005563278908562142
32-33	0.0036342267238804508
34-35	0.0015656442803122445
36-37	0.0
38-39	8.93295495718256E-6
40-41	3.865810003169964E-4
42-43	0.0
44-45	9.122506967086633E-6
46-47	4.143075686811467E-4
48-49	0.001282170517715369
50-51	0.0024455080374292033
52-53	0.0
54-55	0.002824950648968208
56-57	0.03516689173285626
58-59	0.05717458350447998
60-61	0.03836285819684615
62-63	0.012701482184202013
64-65	3.081346759258155E-4
66-67	0.0
68-69	0.0
70-71	0.0027511983168848767
72-73	0.00466834782888406
74-75	0.003787269794128656
76-77	0.0026537074797995846
78-79	0.007400428686259095
80-81	0.01453009466890391
82-83	0.039799561742291344
84-85	0.017584321551800796
86-87	0.0016535868323193207
88-89	9.896218245509705E-4
90-91	5.754672995886214E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	48.0
20-21	237.0
22-23	443.0
24-25	533.0
26-27	1521.0
28-29	3850.0
30-31	11179.0
32-33	14622.0
34-35	19874.0
36-37	31094.0
38-39	35606.0
40-41	35898.0
42-43	41566.0
44-45	49285.0
46-47	50309.0
48-49	46121.0
50-51	41351.0
52-53	38946.0
54-55	41849.0
56-57	42663.0
58-59	45692.0
60-61	46916.0
62-63	47221.0
64-65	52988.0
66-67	58224.0
68-69	59584.0
70-71	71569.0
72-73	49062.0
74-75	47479.0
76-77	49296.0
78-79	50977.0
80-81	51086.0
82-83	47993.0
84-85	47179.0
86-87	48984.0
88-89	50929.0
90-91	51819.0
92-93	51379.0
94-95	59280.0
96-97	176750.0
98-99	1291409.0
100-101	2726599.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.50499419055801
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.69939570037286	81.1585448373918
2	6.091052093352573	10.781770602731115
3	1.3414543755293387	3.5617623513936825
4	0.4475976792648285	1.5845852001216358
5	0.17936833710625505	0.7937496816779577
6	0.085300997035741	0.45297385482582375
7	0.049858832068757186	0.3088928949815354
8	0.029796033995397018	0.21096782525314256
9	0.019933712231895828	0.15878097767521757
>10	0.05404525939006413	0.7098214124102187
>50	0.001343345093101247	0.08029311606376173
>100	7.703571566969733E-4	0.13233382469661348
>500	5.55182907101678E-5	0.034297238241830265
>1k	2.7759111775288098E-5	0.031226182535631995
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.7576514963766015E-5	0.0	0.0	0.0
3	0.0	1.7576514963766015E-5	0.0	0.0	0.0
4	0.0	3.515302992753203E-5	0.0	3.515302992753203E-5	0.0
5	0.0	3.515302992753203E-5	0.0	3.515302992753203E-5	0.0
6	0.0	3.515302992753203E-5	0.0	3.515302992753203E-5	1.7576514963766015E-5
7	0.0	3.515302992753203E-5	0.0	3.515302992753203E-5	1.7576514963766015E-5
8	0.0	8.788257481883008E-5	0.0	3.515302992753203E-5	1.7576514963766015E-5
9	0.0	1.9334166460142617E-4	0.0	5.2729544891298046E-5	1.7576514963766015E-5
10-11	0.0	1.9334166460142617E-4	0.0	7.030605985506406E-5	1.7576514963766015E-5
12-13	0.0	1.9334166460142617E-4	0.0	7.030605985506406E-5	1.7576514963766015E-5
14-15	8.788257481883008E-6	2.460712094927242E-4	0.0	8.788257481883008E-5	1.7576514963766015E-5
16-17	1.7576514963766015E-5	2.460712094927242E-4	0.0	1.0545908978259609E-4	1.7576514963766015E-5
18-19	1.7576514963766015E-5	3.0758901186590527E-4	0.0	1.0545908978259609E-4	1.7576514963766015E-5
20-21	1.7576514963766015E-5	3.515302992753203E-4	0.0	1.0545908978259609E-4	3.515302992753203E-5
22-23	1.7576514963766015E-5	4.1304810164850136E-4	0.0	1.230356047463621E-4	5.2729544891298046E-5
24-25	1.7576514963766015E-5	4.6577764653979936E-4	0.0	1.5818863467389414E-4	7.030605985506406E-5
26-27	1.7576514963766015E-5	4.7456590402168236E-4	0.0	1.8455340711954316E-4	7.030605985506406E-5
28-29	1.7576514963766015E-5	4.7456590402168236E-4	0.0	5.536602213586294E-4	7.030605985506406E-5
30-31	1.7576514963766015E-5	5.272954489129805E-4	0.0	0.0018806871011229635	7.909431733694707E-5
32-33	1.7576514963766015E-5	5.272954489129805E-4	0.0	0.004728082525253058	8.788257481883008E-5
34-35	1.7576514963766015E-5	6.503310536593426E-4	0.0	0.008638857104690995	1.230356047463621E-4
36-37	1.7576514963766015E-5	7.733666584057047E-4	0.0	0.015546427485451039	1.230356047463621E-4
38-39	1.7576514963766015E-5	7.821549158875877E-4	0.0	0.033984191682441586	1.230356047463621E-4
40-41	1.7576514963766015E-5	8.964022631520668E-4	0.0	0.05288773352597194	1.230356047463621E-4
42-43	1.7576514963766015E-5	9.227670355977158E-4	0.0	0.06417185613270972	1.230356047463621E-4
44-45	1.7576514963766015E-5	0.0010106496104165458	0.0	0.0777848669721465	1.230356047463621E-4
46-47	3.515302992753203E-5	0.0010721674127897268	0.0	0.0918460789431593	1.3182386222824512E-4
48-49	3.515302992753203E-5	0.0010897439277534927	0.0	0.1052481716030309	1.5818863467389414E-4
50-51	3.515302992753203E-5	0.001283085592354919	0.0	0.11981031425051104	1.5818863467389414E-4
52-53	3.515302992753203E-5	0.001283085592354919	0.0	0.16050873464911125	1.8455340711954316E-4
54-55	3.515302992753203E-5	0.001283085592354919	0.0	0.21134880418180446	2.197064370470752E-4
56-57	3.515302992753203E-5	0.001318238622282451	0.0	0.28525804960444057	2.2849469452895817E-4
58-59	3.515302992753203E-5	0.001318238622282451	0.0	0.33319799416811235	2.548594669746072E-4
60-61	3.515302992753203E-5	0.001318238622282451	0.0	0.35059874398224067	2.8122423942025625E-4
62-63	3.515302992753203E-5	0.0013358151372462172	0.0	0.3638338597499565	3.163772693477883E-4
64-65	3.515302992753203E-5	0.0014940037719201112	0.0	0.37022292293928544	3.163772693477883E-4
66-67	3.515302992753203E-5	0.0014940037719201112	0.0	0.3763659149191217	3.163772693477883E-4
68-69	3.515302992753203E-5	0.0015027920294019943	0.0	0.3826143659887405	3.163772693477883E-4
70-71	3.515302992753203E-5	0.0015115802868838773	0.0	0.3875094254061493	3.339537843115543E-4
72-73	3.515302992753203E-5	0.0015115802868838773	0.0	0.39138504695565973	3.691068142390863E-4
74-75	3.515302992753203E-5	0.0015467333168114094	0.0	0.3947948908586303	3.691068142390863E-4
76-77	3.515302992753203E-5	0.0016434041491121224	0.0	0.3979147222646988	3.7789507172096933E-4
78-79	3.515302992753203E-5	0.0017049219514853034	0.0	0.3999711745154594	3.8668332920285234E-4
80-81	3.515302992753203E-5	0.0017049219514853034	0.0	0.4005424112517818	3.8668332920285234E-4
82-83	3.515302992753203E-5	0.0017137102089671865	0.0	0.4012894131377419	3.8668332920285234E-4
84-85	3.515302992753203E-5	0.0017400749814128354	0.0	0.4016848847244266	3.9547158668473535E-4
86-87	3.515302992753203E-5	0.0017576514963766015	0.0	0.4018254968441367	4.0425984416661836E-4
88	3.515302992753203E-5	0.0019334166460142615	0.0	0.4018342851016186	4.0425984416661836E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7320	0.0	48.89047	1
GTATCAA	14025	0.0	37.082928	1
CTTATAC	7205	0.0	33.211845	1
ATCAACG	17155	0.0	30.78093	3
TCAACGC	17180	0.0	30.711493	4
CAACGCA	17480	0.0	30.20863	5
TATCAAC	17810	0.0	29.79653	2
AACGCAG	18135	0.0	29.094206	6
TATACAC	9680	0.0	27.034552	3
ACGCAGA	20555	0.0	25.64826	7
CGCAGAG	20790	0.0	25.358343	8
TTATACA	10455	0.0	23.900475	2
GCAGAGT	23995	0.0	21.900652	9
TACACAT	12095	0.0	21.67159	5
ACACATC	11420	0.0	21.28393	6
GTGGTAT	3590	0.0	21.235214	1
ACATCTC	11780	0.0	20.705381	8
TGGTATC	3575	0.0	20.613499	2
GAGTACT	14685	0.0	19.983213	12-13
ATACACA	14670	0.0	19.455206	4
>>END_MODULE