FastQCFastQC Report
Fri 27 May 2016
SRR2140151_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140151_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6006972
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77600.050.2981451
GTATCAA145400.037.5994571
TCAACGC183100.030.3677754
ATCAACG182650.030.3253253
CAACGCA187400.029.625255
TATCAAC189200.029.4386252
AACGCAG192950.028.8175146
GTGGTAT39600.025.3172191
ACGCAGA219300.025.2963457
TGGTATC36450.024.9191722
CGCAGAG222850.024.9125968
GCAGAGT260050.021.2500259
GAGTACT156600.020.01009612-13
AGAGTAC236800.019.1214110-11
CAGAGTA251950.018.8472510-11
TACATGG169450.018.5587962
GTACATG174200.018.4954551
GTACTTT172850.018.1417514-15
ACATGGG173900.017.5144563
CGAGACC74550.017.39896486-87
Produced by FastQC (version 0.11.5)