##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140151_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6006972
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.032819197425923	18.0	18.0	28.0	18.0	33.0
2	28.495463771097985	29.0	27.0	31.0	25.0	33.0
3	30.376007745666204	31.0	29.0	33.0	27.0	33.0
4	31.36595825650594	33.0	31.0	33.0	29.0	33.0
5	32.315807864594674	33.0	33.0	33.0	31.0	34.0
6	36.03412867581204	38.0	36.0	38.0	33.0	38.0
7	36.21009586860069	38.0	36.0	38.0	33.0	38.0
8	36.043736178560515	38.0	36.0	38.0	33.0	38.0
9	36.85006489126302	38.0	38.0	38.0	35.0	38.0
10-11	36.96136780727461	38.0	38.0	38.0	35.0	38.0
12-13	37.11622145067432	38.0	38.0	38.0	36.0	38.0
14-15	37.02499021137439	38.0	38.0	38.0	35.5	38.0
16-17	37.1194729224641	38.0	38.0	38.0	36.0	38.0
18-19	37.22962450965312	38.0	38.0	38.0	36.0	38.0
20-21	37.285029944451836	38.0	38.0	38.0	36.5	38.0
22-23	37.25985150431998	38.0	38.0	38.0	36.5	38.0
24-25	37.32946353295347	38.0	38.0	38.0	37.0	38.0
26-27	37.31342204365163	38.0	38.0	38.0	37.0	38.0
28-29	37.29029928959724	38.0	38.0	38.0	37.0	38.0
30-31	37.29699964518861	38.0	38.0	38.0	37.0	38.0
32-33	37.28257712869335	38.0	38.0	38.0	37.0	38.0
34-35	37.19580015924136	38.0	38.0	38.0	36.0	38.0
36-37	37.21920407248757	38.0	38.0	38.0	36.5	38.0
38-39	37.20085883282116	38.0	38.0	38.0	36.0	38.0
40-41	37.20577644823051	38.0	38.0	38.0	37.0	38.0
42-43	37.18347961306527	38.0	38.0	38.0	36.0	38.0
44-45	37.144801140916925	38.0	38.0	38.0	36.0	38.0
46-47	37.099052399907514	38.0	38.0	38.0	36.0	38.0
48-49	37.040357889885	38.0	38.0	38.0	36.0	38.0
50-51	36.99359022560512	38.0	38.0	38.0	36.0	38.0
52-53	36.89405248901792	38.0	38.0	38.0	36.0	38.0
54-55	36.921961993981604	38.0	38.0	38.0	36.0	38.0
56-57	36.87715176671135	38.0	38.0	38.0	35.5	38.0
58-59	36.85538992937147	38.0	38.0	38.0	35.0	38.0
60-61	36.85203542276501	38.0	38.0	38.0	35.0	38.0
62-63	36.85673352192447	38.0	38.0	38.0	35.0	38.0
64-65	36.86316322109816	38.0	38.0	38.0	35.0	38.0
66-67	36.86021562897684	38.0	38.0	38.0	35.0	38.0
68-69	36.840678674205165	38.0	38.0	38.0	35.0	38.0
70-71	36.838740493775845	38.0	38.0	38.0	35.0	38.0
72-73	36.84811318819042	38.0	38.0	38.0	35.0	38.0
74-75	36.78170687106026	38.0	38.0	38.0	35.0	38.0
76-77	36.77834504904634	38.0	38.0	38.0	35.0	38.0
78-79	36.77456472663685	38.0	38.0	38.0	35.0	38.0
80-81	36.74879288760498	38.0	38.0	38.0	35.0	38.0
82-83	36.692256357276534	38.0	38.0	38.0	34.0	38.0
84-85	36.683207204392616	38.0	38.0	38.0	34.0	38.0
86-87	36.668393998327815	38.0	38.0	38.0	34.0	38.0
88-89	36.64298761833897	38.0	38.0	38.0	34.0	38.0
90-91	36.65086122105995	38.0	38.0	38.0	34.0	38.0
92-93	36.61914121796788	38.0	38.0	38.0	34.0	38.0
94-95	36.54380100162548	38.0	38.0	38.0	34.0	38.0
96-97	36.55750395496378	38.0	38.0	38.0	34.0	38.0
98-99	36.58069153994039	38.0	38.0	38.0	34.0	38.0
100	34.91699791118323	38.0	34.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	8.0
19	9.0
20	35.0
21	126.0
22	483.0
23	1454.0
24	3395.0
25	6778.0
26	12200.0
27	19990.0
28	31429.0
29	45223.0
30	61599.0
31	80540.0
32	106806.0
33	149022.0
34	229356.0
35	428668.0
36	1320299.0
37	3509550.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.48021199366336	17.569234549453537	18.39437573539547	23.556177721487632
2	14.296482035554266	21.109917865878124	41.90476904044768	22.688831058119924
3	18.08540143020477	25.570337268094473	31.508753495105353	24.835507806595402
4	12.523131454583108	18.027518689948945	38.85796371283236	30.591386142635592
5	13.425849163272277	36.99056696119109	35.7180456309768	13.865538244559822
6	29.6500299984751	38.034553848428125	18.776498375554272	13.538917777542494
7	26.253876329038988	32.79197905367297	23.060636873286576	17.893507744001468
8	24.215045450519828	36.88076122212656	21.21822775268471	17.685965574668902
9	25.40985707940706	17.142663558278613	21.216746140984178	36.23073322133015
10-11	24.089915851114338	26.81018822794579	28.944707916068197	20.155188004871672
12-13	25.04650429534215	24.21999802895702	29.118730701591417	21.61476697410942
14-15	22.168540156338334	25.61109823718173	26.82028482902867	25.400076777451268
16-17	21.689912987774875	28.863144359587494	27.409025712122514	22.03791694051512
18-19	21.7300913005754	27.752285177956548	29.350935879175065	21.166687642292988
20-21	22.513061462685716	26.93288713449567	29.24251881818682	21.31153258463179
22-23	22.207001011470126	26.872507899014806	29.166191842028528	21.754299247486536
24-25	22.04960593370469	26.90936841502367	29.312168836462156	21.728856814809486
26-27	21.94417487368103	27.13379320588427	29.22334313334994	21.69868878708476
28-29	21.760140151300305	27.14539423091899	29.269551965097435	21.82491365268327
30-31	22.222182391710867	26.92123420971481	29.240598232420094	21.61598516615423
32-33	22.080702972021182	27.0625352611444	29.202612675929885	21.654149090904536
34-35	21.956349386763215	27.13628556757025	29.255844364294376	21.65152068137216
36-37	22.082794902563112	27.074160541714647	29.17745874234393	21.665585813378303
38-39	21.772340744967632	27.196261990189107	29.282839830345154	21.7485574344981
40-41	22.065146028903648	27.03885279485923	29.14834568283157	21.747655493405553
42-43	22.035545643760273	27.1035533672075	29.015542667941368	21.84535832109086
44-45	21.95706894502075	27.286331081511804	28.932340257641464	21.82425971582598
46-47	22.175732144861822	27.10896229907094	28.5712448608837	22.144060695183544
48-49	22.059185660416382	27.274013886345095	28.568074726518923	22.098725726719604
50-51	21.971604651567482	27.484358902491262	28.519387169843586	22.02464927609767
52-53	22.167031358712055	27.412280643227987	28.44346196878905	21.97722602927091
54-55	22.10132962544538	27.327570219471966	28.426220167195403	22.144879987887247
56-57	22.15737566955498	27.489706100128945	28.28183975468011	22.071078475635964
58-59	21.971821616997396	27.51272636333252	28.394907019260252	22.120545000409837
60-61	22.25218801288698	27.560886063815854	28.40030117418419	21.786624749112978
62-63	21.880439747643205	27.718752152843173	28.364612021577724	22.036196077935895
64-65	21.804858936574398	27.642775284761946	28.440167325572407	22.112198453091246
66-67	21.90220791940359	27.673241294920732	28.5160913280276	21.908459457648082
68-69	21.92285087708071	27.797316285957297	28.410985037563712	21.868847799398285
70-71	21.97266798714081	27.36172448581155	28.24554953378624	22.420057993261402
72-73	22.050357513488912	27.33662452593003	28.29061056908545	22.322407391495602
74-75	21.905370058917402	27.58902033348336	28.390673555679037	22.114936051920203
76-77	22.107826768595356	27.291648904929712	28.215377638209997	22.38514668826494
78-79	22.122573057312188	27.360373152170915	27.923137126865022	22.59391666365188
80-81	22.324297348494763	27.54117185986403	27.881003084138538	22.25352770750267
82-83	22.350372548608583	27.3525735882971	27.943363982361564	22.35368988073275
84-85	22.302403129452454	27.29448303330385	27.887121345283216	22.51599249196048
86-87	22.0647418419209	27.588131236278034	27.943680900063338	22.40344602173773
88-89	22.424536040901923	27.637659817681776	27.839662744397764	22.09814139701854
90-91	22.497435763790673	27.593808881823513	27.857086709851348	22.051668644534463
92-93	22.280705626098214	27.38795229976832	27.783261638431867	22.548080435701596
94-95	22.38940774924425	27.694918347498948	27.775126218209188	22.14054768504761
96-97	22.637149135125313	27.5397578020453	27.96467808818913	21.858414974640255
98-99	23.311297512308006	29.657637904404787	28.250931889625015	18.780132693662193
100	22.249445870659574	30.48489231090431	22.23700519822476	25.028656620211354
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1483.0
1	1367.5
2	1592.5
3	2061.0
4	2582.0
5	3500.0
6	4387.0
7	6113.0
8	7814.0
9	8288.0
10	8177.5
11	8027.0
12	8859.0
13	11107.5
14	15481.0
15	20670.0
16	24393.0
17	26198.5
18	25902.0
19	23837.0
20	21261.5
21	19341.5
22	19376.5
23	22011.0
24	27337.0
25	35756.0
26	47359.0
27	60169.5
28	71588.5
29	83631.0
30	95661.5
31	107780.5
32	122045.0
33	136489.5
34	151717.5
35	166164.5
36	181695.0
37	195412.0
38	203933.5
39	212045.5
40	222593.0
41	231589.0
42	237252.0
43	243784.0
44	247738.0
45	251635.0
46	259668.0
47	264560.5
48	262517.5
49	255870.5
50	246666.0
51	236858.0
52	225876.0
53	213050.5
54	199187.0
55	183559.5
56	167113.0
57	151005.0
58	134649.0
59	117191.0
60	98254.0
61	79584.0
62	62321.0
63	47704.0
64	35843.5
65	26385.5
66	19390.5
67	14576.0
68	10845.5
69	7627.5
70	5414.5
71	3962.0
72	2798.5
73	1837.0
74	1144.0
75	719.0
76	476.5
77	305.5
78	189.5
79	113.0
80	71.0
81	41.0
82	28.5
83	22.0
84	16.0
85	11.0
86	8.0
87	5.0
88	1.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.015681777774226347
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.161396021096457E-4
26-27	3.996365971210179E-4
28-29	0.003914408379232548
30-31	0.006293750947188677
32-33	0.004534936044874319
34-35	0.0020010603107855976
36-37	0.0
38-39	0.0
40-41	4.249393505311955E-4
42-43	0.0
44-45	2.5843392658564073E-5
46-47	4.6051552888774815E-4
48-49	0.0015247931363978288
50-51	0.0026587855904067485
52-53	0.0
54-55	0.0027503792522139883
56-57	0.03812706566737168
58-59	0.060938883839407605
60-61	0.04167212939095607
62-63	0.013969913877094634
64-65	4.463880095716749E-4
66-67	0.0
68-69	9.480876503240374E-6
70-71	0.002817732932589094
72-73	0.0051047915879234
74-75	0.0039786334674631356
76-77	0.0033154143083808938
78-79	0.007852784604396543
80-81	0.016028345202228986
82-83	0.04188666148864259
84-85	0.0188168958880255
86-87	0.0019293100787158512
88-89	0.0011527321953700826
90-91	1.271257814262877E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	43.0
20-21	221.0
22-23	479.0
24-25	538.0
26-27	1465.0
28-29	3835.0
30-31	10432.0
32-33	13923.0
34-35	19211.0
36-37	30270.0
38-39	34706.0
40-41	35127.0
42-43	40676.0
44-45	48890.0
46-47	49751.0
48-49	46242.0
50-51	41128.0
52-53	38843.0
54-55	41480.0
56-57	43232.0
58-59	25429.0
60-61	47257.0
62-63	45607.0
64-65	48869.0
66-67	52213.0
68-69	54458.0
70-71	58751.0
72-73	47556.0
74-75	47058.0
76-77	49260.0
78-79	50857.0
80-81	51225.0
82-83	48304.0
84-85	47321.0
86-87	48556.0
88-89	50885.0
90-91	52771.0
92-93	52456.0
94-95	60946.0
96-97	208840.0
98-99	411129.0
100-101	3946732.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.73403918431889
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.00329076501538	78.93082987114809
2	6.399228708546019	11.100619071128977
3	1.4443735379549165	3.7582905311332517
4	0.5276643355050088	1.8306583660743607
5	0.23838814251524384	1.033818324699708
6	0.12228863772297992	0.6363952497637151
7	0.0755148277314798	0.4584794219221482
8	0.05115471443766003	0.3549484005198919
9	0.030178835658721096	0.23557790831045727
>10	0.10572045753500017	1.3571322772257606
>50	0.0014214978269903686	0.0841098622457065
>100	6.590581670438063E-4	0.11151956932957369
>500	2.9120354898828247E-5	0.014371380956252016
>1k	8.73610286434189E-5	0.09324976554214559
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.664732247794729E-5	0.0	0.0	0.0
3	0.0	1.664732247794729E-5	0.0	0.0	0.0
4	0.0	1.664732247794729E-5	0.0	1.664732247794729E-5	1.664732247794729E-5
5	0.0	1.664732247794729E-5	0.0	3.329464495589458E-5	1.664732247794729E-5
6	0.0	1.664732247794729E-5	0.0	3.329464495589458E-5	3.329464495589458E-5
7	0.0	1.664732247794729E-5	0.0	3.329464495589458E-5	4.994196743384187E-5
8	0.0	1.664732247794729E-5	0.0	3.329464495589458E-5	4.994196743384187E-5
9	0.0	1.664732247794729E-5	0.0	4.994196743384187E-5	9.988393486768375E-5
10-11	0.0	1.664732247794729E-5	0.0	7.491295115076281E-5	1.0820759610665739E-4
12-13	0.0	1.664732247794729E-5	0.0	9.988393486768375E-5	1.2485491858460468E-4
14-15	0.0	9.15602736287101E-5	0.0	9.988393486768375E-5	1.4982590230152562E-4
16-17	0.0	9.988393486768375E-5	0.0	1.0820759610665739E-4	1.664732247794729E-4
18-19	0.0	1.3317857982357833E-4	0.0	1.4150224106255197E-4	1.9144420849639385E-4
20-21	0.0	1.4982590230152562E-4	0.0	1.5814956354049927E-4	1.997678697353675E-4
22-23	0.0	1.5814956354049927E-4	0.0	2.1641519221331478E-4	2.0809153097434114E-4
24-25	0.0	1.9144420849639385E-4	0.0	2.1641519221331478E-4	2.1641519221331478E-4
26-27	0.0	2.1641519221331478E-4	0.0	2.4970983716920937E-4	2.1641519221331478E-4
28-29	0.0	2.1641519221331478E-4	0.0	5.660089642502079E-4	2.1641519221331478E-4
30-31	0.0	2.3306251469126208E-4	0.0	0.0016314376028388345	2.2473885345228843E-4
32-33	0.0	2.3306251469126208E-4	0.0	0.0037706185412550615	2.4970983716920937E-4
34-35	0.0	2.8300448212510395E-4	0.0	0.006783783909763521	2.4970983716920937E-4
36-37	0.0	3.1629912708099853E-4	0.0	0.011594860105890288	2.4970983716920937E-4
38-39	0.0	3.1629912708099853E-4	0.0	0.026544155691086955	2.4970983716920937E-4
40-41	0.0	3.662410945148404E-4	0.0	0.04269205849469583	2.4970983716920937E-4
42-43	0.0	3.662410945148404E-4	0.0	0.051922998808717605	2.4970983716920937E-4
44-45	0.0	4.0785940070970863E-4	0.0	0.0633597093510674	2.4970983716920937E-4
46-47	0.0	4.4947770690457686E-4	0.0	0.07496289311819665	2.6635715964715666E-4
48-49	0.0	4.578013681435505E-4	0.0	0.08666596082019361	2.8300448212510395E-4
50-51	0.0	6.076272704450762E-4	0.0	0.09876024060042231	2.8300448212510395E-4
52-53	0.0	6.159509316840498E-4	0.0	0.13699914033226723	2.8300448212510395E-4
54-55	0.0	6.159509316840498E-4	0.0	0.18585070814380356	2.8300448212510395E-4
56-57	0.0	6.325982541619971E-4	0.0	0.24558130119467847	2.913281433640776E-4
58-59	0.0	6.492455766399444E-4	0.0	0.45465502419521847	2.9965180460305124E-4
60-61	0.0	6.575692378789179E-4	0.0	0.6382084018370653	3.079754658420249E-4
62-63	0.0	6.658928991178916E-4	0.0	0.6456747259684248	3.329464495589458E-4
64-65	0.0	7.158348665517335E-4	0.0	0.6521422107511072	3.329464495589458E-4
66-67	0.0	7.158348665517335E-4	0.0	0.6583266910516646	3.329464495589458E-4
68-69	0.0	7.491295115076281E-4	0.0	0.6639618097104498	3.329464495589458E-4
70-71	0.0	7.741004952245491E-4	0.0	0.6689143881476391	3.329464495589458E-4
72-73	0.0	7.824241564635227E-4	0.0	0.6722105579982727	3.495937720368931E-4
74-75	0.0	7.9907147894147E-4	0.0	0.6754567858814724	3.495937720368931E-4
76-77	0.0	8.323661238973646E-4	0.0	0.6785781588460875	3.495937720368931E-4
78-79	0.0	8.490134463753118E-4	0.0	0.6805258955760074	3.495937720368931E-4
80-81	0.0	8.490134463753118E-4	0.0	0.681283348748754	3.495937720368931E-4
82-83	0.0	8.490134463753118E-4	0.0	0.681874328696721	3.495937720368931E-4
84-85	0.0	8.573371076142854E-4	0.0	0.682173980501324	3.495937720368931E-4
86-87	0.0	8.656607688532591E-4	0.0	0.6823404537261036	3.495937720368931E-4
88	0.0	9.15602736287101E-4	0.0	0.6823404537261036	3.495937720368931E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7760	0.0	50.298145	1
GTATCAA	14540	0.0	37.599457	1
TCAACGC	18310	0.0	30.367775	4
ATCAACG	18265	0.0	30.325325	3
CAACGCA	18740	0.0	29.62525	5
TATCAAC	18920	0.0	29.438625	2
AACGCAG	19295	0.0	28.817514	6
GTGGTAT	3960	0.0	25.317219	1
ACGCAGA	21930	0.0	25.296345	7
TGGTATC	3645	0.0	24.919172	2
CGCAGAG	22285	0.0	24.912596	8
GCAGAGT	26005	0.0	21.250025	9
GAGTACT	15660	0.0	20.010096	12-13
AGAGTAC	23680	0.0	19.12141	10-11
CAGAGTA	25195	0.0	18.84725	10-11
TACATGG	16945	0.0	18.558796	2
GTACATG	17420	0.0	18.495455	1
GTACTTT	17285	0.0	18.14175	14-15
ACATGGG	17390	0.0	17.514456	3
CGAGACC	7455	0.0	17.398964	86-87
>>END_MODULE