FastQCFastQC Report
Fri 27 May 2016
SRR2140150_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140150_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6382023
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73850.050.8756141
GTATCAA143900.038.804191
TCAACGC178650.031.976914
ATCAACG180450.031.5861513
CAACGCA184250.031.005025
TATCAAC189250.030.5095942
AACGCAG189650.030.1221986
ACGCAGA214000.026.6745647
CGCAGAG220150.025.8705588
GCAGAGT256450.022.1076039
GTGGTAT36400.021.711541
ATCTCGT126050.021.5158694
TGGTATC36950.020.8039822
GAGTACT150650.020.67982512-13
AGAGTAC230750.019.92909410-11
TACATGG179650.019.255122
CAGAGTA250100.019.1900210-11
GTACATG181250.019.0851421
ACATGGG179150.018.94463
GTACTTT167950.018.54980714-15
Produced by FastQC (version 0.11.5)