##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140150_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6382023
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	21.695899717064638	18.0	18.0	25.0	18.0	33.0
2	28.299327344950026	29.0	27.0	31.0	25.0	33.0
3	30.24409673860467	31.0	29.0	33.0	27.0	33.0
4	31.324984413249528	33.0	31.0	33.0	29.0	33.0
5	32.29663274482088	33.0	33.0	33.0	31.0	33.0
6	35.96350404879456	38.0	36.0	38.0	33.0	38.0
7	36.093315082067235	38.0	36.0	38.0	33.0	38.0
8	35.91602929039898	38.0	36.0	38.0	31.0	38.0
9	36.786508603933264	38.0	37.0	38.0	34.0	38.0
10-11	36.916333504282264	38.0	38.0	38.0	35.0	38.0
12-13	37.082797883993834	38.0	38.0	38.0	36.0	38.0
14-15	36.984041737235984	38.0	38.0	38.0	35.5	38.0
16-17	37.07560478863834	38.0	38.0	38.0	36.0	38.0
18-19	37.20679555683206	38.0	38.0	38.0	36.0	38.0
20-21	37.26950633624725	38.0	38.0	38.0	36.0	38.0
22-23	37.25347269338842	38.0	38.0	38.0	36.5	38.0
24-25	37.32565702939644	38.0	38.0	38.0	37.0	38.0
26-27	37.311384401878726	38.0	38.0	38.0	37.0	38.0
28-29	37.294090270834495	38.0	38.0	38.0	37.0	38.0
30-31	37.29994913544323	38.0	38.0	38.0	37.0	38.0
32-33	37.278979359679724	38.0	38.0	38.0	37.0	38.0
34-35	37.18915659705516	38.0	38.0	38.0	36.0	38.0
36-37	37.2168659041853	38.0	38.0	38.0	36.0	38.0
38-39	37.19277850836298	38.0	38.0	38.0	36.0	38.0
40-41	37.205988857096045	38.0	38.0	38.0	36.0	38.0
42-43	37.18733322744242	38.0	38.0	38.0	36.0	38.0
44-45	37.150934487649	38.0	38.0	38.0	36.0	38.0
46-47	37.10607043176837	38.0	38.0	38.0	36.0	38.0
48-49	37.049529629027944	38.0	38.0	38.0	36.0	38.0
50-51	36.99831986743533	38.0	38.0	38.0	36.0	38.0
52-53	36.89767596717806	38.0	38.0	38.0	36.0	38.0
54-55	36.9245220316621	38.0	38.0	38.0	36.0	38.0
56-57	36.878739660290634	38.0	38.0	38.0	35.5	38.0
58-59	36.85549779386427	38.0	38.0	38.0	35.0	38.0
60-61	36.842383212431315	38.0	38.0	38.0	35.0	38.0
62-63	36.84896228499971	38.0	38.0	38.0	35.0	38.0
64-65	36.85722341469962	38.0	38.0	38.0	35.0	38.0
66-67	36.844559150698416	38.0	38.0	38.0	35.0	38.0
68-69	36.82083812583896	38.0	38.0	38.0	35.0	38.0
70-71	36.817604202504114	38.0	38.0	38.0	35.0	38.0
72-73	36.82689499831447	38.0	38.0	38.0	35.0	38.0
74-75	36.7557771911919	38.0	38.0	38.0	35.0	38.0
76-77	36.74574983433703	38.0	38.0	38.0	35.0	38.0
78-79	36.739059069791864	38.0	38.0	38.0	35.0	38.0
80-81	36.71004916106832	38.0	38.0	38.0	34.0	38.0
82-83	36.62707179236122	38.0	38.0	38.0	34.0	38.0
84-85	36.64091634001265	38.0	38.0	38.0	34.0	38.0
86-87	36.631890703638575	38.0	38.0	38.0	34.0	38.0
88-89	36.60428704216458	38.0	38.0	38.0	34.0	38.0
90-91	36.619634169999216	38.0	38.0	38.0	34.0	38.0
92-93	36.587402509359634	38.0	38.0	38.0	34.0	38.0
94-95	36.481280449604185	38.0	38.0	38.0	34.0	38.0
96-97	36.43908233521563	38.0	37.0	38.0	34.0	38.0
98-99	36.49056064919819	38.0	37.0	38.0	34.0	38.0
100	34.80933492566503	38.0	34.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	9.0
19	12.0
20	42.0
21	133.0
22	521.0
23	1526.0
24	3595.0
25	7339.0
26	13065.0
27	21226.0
28	33035.0
29	47738.0
30	64447.0
31	84900.0
32	112929.0
33	159743.0
34	249562.0
35	477997.0
36	1486760.0
37	3617443.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.94424651869791	17.33525560782216	18.774532777459434	23.945965096020494
2	14.31605621618911	21.05392817516784	41.74802697975891	22.88198862888414
3	18.105497269439486	25.50916848779768	31.480503909183653	24.90483033357918
4	12.56805248116467	18.092037587454637	38.420137313826665	30.919772617554024
5	13.49935592522935	36.88476208876089	35.73591007114828	13.879971914861478
6	29.81947887057129	37.778005500763626	18.68907398171395	13.71344164695113
7	26.328877222786566	32.533430230508415	22.878309902675063	18.259382644029955
8	24.272585667585343	36.6793883381492	21.264244895388188	17.78378109887727
9	25.401929764276936	16.934489267744727	21.04332748409086	36.620253483887474
10-11	24.256673785099174	26.536844195014652	28.90935993179592	20.29712208809025
12-13	25.150160066800137	23.941538599907897	28.93935825677845	21.968943076513515
14-15	22.30297509112706	25.343421670526727	26.432096844527198	25.921506393819012
16-17	21.975970628748907	28.63951602806822	27.07315533021426	22.311358012968615
18-19	22.02494412821765	27.496163833944188	28.893448049309754	21.585443988528404
20-21	22.72048938097193	26.7037374215364	28.757579983515985	21.818193213975686
22-23	22.40237631873522	26.826265774659174	28.649803016797183	22.12155488980842
24-25	22.3249345459999	26.899020074440553	28.81525086429682	21.960794515262727
26-27	22.23017449140175	27.069668826343808	28.749579377812996	21.950577304441442
28-29	22.172922528393034	26.865191203667045	28.85945732793865	22.10242894000127
30-31	22.230839325477014	26.61124723465934	28.98540044534257	22.172512994521078
32-33	21.963573316818675	27.05912629034208	28.766762246250522	22.210538146588725
34-35	22.30062416213892	26.90581155749009	28.776775666037445	22.01678861433355
36-37	22.131739992833865	26.927277030064506	28.976080567385594	21.96490240971604
38-39	21.939195440680418	26.851282766111545	28.983427577910003	22.226094215298037
40-41	22.34616063076648	26.638428284633036	28.987633933205036	22.02777715139544
42-43	22.297651320135774	26.692278608187443	28.8073003531959	22.202769718480887
44-45	22.06998442594797	27.00087791640308	28.707160608080823	22.221977049568125
46-47	22.444520726552707	26.728612322048654	28.399542438306845	22.427324513091797
48-49	22.26508663696687	26.97917692672934	28.327028202309872	22.42870823399391
50-51	22.02991923768512	27.153264281938377	28.319647934938015	22.49716854543849
52-53	22.132361015476604	26.972333098915215	28.38913864190802	22.506167243700165
54-55	22.237695411644037	26.710465896647946	28.507059339973694	22.54477935173432
56-57	22.32394910693053	26.633744346874643	28.312306207665554	22.73000033852927
58-59	21.82809577407382	26.64343665226775	28.740797244992017	22.787670328666415
60-61	22.168762158593267	27.178047859287744	28.605464393644336	22.047725588474652
62-63	21.534247068448973	27.64798516117853	28.56629345199138	22.251474318381117
64-65	21.315338225229553	27.94050701072075	28.23453287780376	22.50962188624594
66-67	21.437263991479558	28.464543661208637	27.775129749539012	22.323062597772797
68-69	21.60583062966661	28.322859404110602	27.67422433626356	22.397085629959232
70-71	21.652836977828542	27.499230142253417	27.71510914798247	23.132823731935577
72-73	22.009980673831773	26.746667235733884	27.842961478393587	23.400390612040756
74-75	22.004837302262597	26.927018650407152	27.97432536650892	23.093818680821325
76-77	22.41003655696531	26.806887750789414	27.24628410624137	23.536791586003904
78-79	22.623059435732845	26.935769075379028	26.737832420093138	23.70333906879499
80-81	22.446345142603036	27.28386734665517	26.9095857898543	23.360201720887485
82-83	22.473527842658207	27.02907648601316	26.962568548607535	23.534827122721094
84-85	22.547729010127114	27.424030942907336	26.91478700247913	23.113453044486423
86-87	22.99357028090492	27.312569984080646	26.9584083761906	22.735451358823834
88-89	22.700178032692147	27.523974603154876	27.293345756266095	22.482501607886878
90-91	22.74775378272696	27.080849491738164	27.135892746529837	23.035503979005043
92-93	22.74353502260554	26.68083656170345	27.305917692738557	23.269710722952453
94-95	21.979752901378628	27.182193766348306	27.929050012108743	22.909003320164327
96-97	22.124475699923313	26.965699779394104	28.204412138315497	22.705412382367086
98-99	23.313597504653224	28.91833089729461	28.42759777117208	19.340473826880086
100	22.357706756993917	29.884541683847964	22.161495155706547	25.596256403451573
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1449.0
1	1366.0
2	1619.0
3	2111.0
4	2696.5
5	3695.0
6	4582.5
7	6067.0
8	7544.0
9	7992.5
10	7926.5
11	7810.5
12	8469.0
13	10575.0
14	15104.0
15	20346.0
16	23852.0
17	25489.5
18	25120.0
19	23015.5
20	20476.0
21	18603.5
22	18470.0
23	20775.5
24	25463.0
25	32932.5
26	43527.0
27	56013.5
28	67525.0
29	79745.5
30	91872.0
31	104000.0
32	118861.5
33	133654.0
34	148189.0
35	162106.5
36	178074.5
37	194320.5
38	205269.0
39	214023.0
40	225638.0
41	236304.0
42	244053.5
43	252343.0
44	264517.0
45	283688.0
46	288903.5
47	281792.5
48	277589.5
49	269985.0
50	262737.5
51	255788.0
52	246634.5
53	234858.0
54	220725.5
55	205299.0
56	188327.0
57	169779.5
58	150982.0
59	131716.0
60	110413.0
61	88811.0
62	69663.0
63	53687.0
64	40048.5
65	29033.5
66	21088.0
67	16070.0
68	12231.0
69	8862.5
70	6441.5
71	4605.5
72	3076.5
73	1951.5
74	1280.5
75	842.5
76	546.5
77	325.0
78	190.5
79	108.5
80	71.0
81	49.5
82	33.5
83	24.5
84	15.5
85	9.5
86	7.5
87	6.0
88	3.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.5
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.014775879059038176
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	2.9774879968056257E-4
26-27	3.2129397779889963E-4
28-29	0.003692310297367383
30-31	0.0061210647733428575
32-33	0.004176205350387561
34-35	0.0017746801415106284
36-37	0.0
38-39	0.0
40-41	3.678316802231299E-4
42-43	0.0
44-45	0.0
46-47	4.0923710346561624E-4
48-49	0.0014695840276361056
50-51	0.0026468103729330845
52-53	0.0
54-55	0.0030310833843523954
56-57	0.03878625589554014
58-59	0.06110936787630441
60-61	0.04187713940761689
62-63	0.013522431192216649
64-65	2.483392739227578E-4
66-67	0.0
68-69	0.0
70-71	0.0026037651329456793
72-73	0.004805756866359612
74-75	0.004380897693004792
76-77	0.0029149686289065835
78-79	0.00791905434307983
80-81	0.015171904579444038
82-83	0.041719711376479125
84-85	0.019352700686507084
86-87	0.0018352992815812248
88-89	0.0012136679988857557
90-91	1.2759654052407236E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	42.0
20-21	247.0
22-23	415.0
24-25	590.0
26-27	1706.0
28-29	4431.0
30-31	13407.0
32-33	16858.0
34-35	23184.0
36-37	35752.0
38-39	22322.0
40-41	40923.0
42-43	45489.0
44-45	54014.0
46-47	53826.0
48-49	49995.0
50-51	25563.0
52-53	4334.0
54-55	3891.0
56-57	3838.0
58-59	26860.0
60-61	51449.0
62-63	50810.0
64-65	57351.0
66-67	63196.0
68-69	64621.0
70-71	76607.0
72-73	53521.0
74-75	51742.0
76-77	53827.0
78-79	55246.0
80-81	55909.0
82-83	52292.0
84-85	51105.0
86-87	53298.0
88-89	54979.0
90-91	56377.0
92-93	56633.0
94-95	67178.0
96-97	222542.0
98-99	500499.0
100-101	4205154.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.613054164668
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.56852152312936	78.44416260296703
2	6.773199895750505	11.732950588775243
3	1.5306147240908572	3.977136480087593
4	0.5347380772894493	1.8526119220872599
5	0.24636942935317258	1.066940436454234
6	0.12517786601859932	0.6505222373811897
7	0.06610178458178337	0.4007694213854254
8	0.04112325289294727	0.28494484241953455
9	0.027206041295769187	0.21207584955209863
>10	0.08499549591691466	1.1247511963121382
>50	0.0013207263336952602	0.07664983150388195
>100	5.512657898665581E-4	0.10825771756676646
>500	5.327839248011862E-5	0.035647805954010084
>1k	2.6639164546962925E-5	0.032579067553523355
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5669012787951408E-5
2	0.0	0.0	0.0	0.0	1.5669012787951408E-5
3	0.0	1.5669012787951408E-5	0.0	0.0	1.5669012787951408E-5
4	0.0	4.700703836385422E-5	0.0	0.0	1.5669012787951408E-5
5	0.0	6.267605115180563E-5	0.0	0.0	1.5669012787951408E-5
6	1.8802815345541688E-4	6.267605115180563E-5	0.0	0.0	6.267605115180563E-5
7	1.8802815345541688E-4	7.834506393975704E-5	0.0	0.0	6.267605115180563E-5
8	2.036971662433683E-4	1.2535210230361126E-4	0.0	0.0	6.267605115180563E-5
9	2.036971662433683E-4	1.8802815345541688E-4	0.0	0.0	7.834506393975704E-5
10-11	2.036971662433683E-4	1.8802815345541688E-4	0.0	0.0	7.834506393975704E-5
12-13	2.350351918192711E-4	1.8802815345541688E-4	0.0	0.0	7.834506393975704E-5
14-15	2.507042046072225E-4	2.272006854252954E-4	0.0	0.0	9.401407672770844E-5
16-17	3.6038729412288234E-4	2.350351918192711E-4	0.0	0.0	1.4102111509156265E-4
18-19	3.6038729412288234E-4	2.4286969821324682E-4	0.0	0.0	1.4102111509156265E-4
20-21	3.6038729412288234E-4	2.5853871100119824E-4	0.0	0.0	1.4102111509156265E-4
22-23	3.6038729412288234E-4	2.89876736577101E-4	0.0	0.0	1.723591406674655E-4
24-25	3.6822180051685805E-4	3.2904926854697956E-4	0.0	3.1338025575902816E-5	1.8802815345541688E-4
26-27	3.7605630691083376E-4	3.447182813349309E-4	0.0	1.6452463427348978E-4	1.8802815345541688E-4
28-29	3.7605630691083376E-4	3.5255278772890663E-4	0.0	6.267605115180563E-4	1.8802815345541688E-4
30-31	3.7605630691083376E-4	4.2306334527468796E-4	0.0	0.0021466547519493426	1.8802815345541688E-4
32-33	3.7605630691083376E-4	4.2306334527468796E-4	0.0	0.0057348586803902145	2.036971662433683E-4
34-35	3.8389081330480947E-4	4.622358772445665E-4	0.0	0.010388555478411783	2.193661790313197E-4
36-37	3.917253196987852E-4	5.01408409214445E-4	0.0	0.021873941851980164	2.272006854252954E-4
38-39	3.917253196987852E-4	5.01408409214445E-4	0.0	0.18852956186463132	2.350351918192711E-4
40-41	3.917253196987852E-4	5.484154475782992E-4	0.0	0.36184451231216186	2.507042046072225E-4
42-43	3.917253196987852E-4	5.484154475782992E-4	0.0	0.387573031309978	2.507042046072225E-4
44-45	4.152288388807123E-4	6.267605115180563E-4	0.0	0.42102637361225426	2.507042046072225E-4
46-47	4.387323580626394E-4	6.894365626698619E-4	0.0	0.4591960887637039	2.820422301831253E-4
48-49	4.387323580626394E-4	6.894365626698619E-4	0.0	0.4964726701862403	2.977112429710767E-4
50-51	4.622358772445665E-4	7.521126138216675E-4	0.0	0.5350497796701768	2.977112429710767E-4
52-53	4.700703836385422E-4	7.521126138216675E-4	0.0	1.0407514983885204	3.368837749409552E-4
54-55	4.700703836385422E-4	7.599471202156432E-4	0.0	1.714824594019796	3.7605630691083376E-4
56-57	4.700703836385422E-4	7.677816266096189E-4	0.0	2.4514875612325433	3.7605630691083376E-4
58-59	4.700703836385422E-4	7.677816266096189E-4	0.0	3.2011088020209266	3.7605630691083376E-4
60-61	4.700703836385422E-4	7.677816266096189E-4	0.0	3.398710722289782	3.917253196987852E-4
62-63	5.170774220023965E-4	7.677816266096189E-4	0.0	3.4064747181262116	4.073943324867366E-4
64-65	5.170774220023965E-4	9.24471754489133E-4	0.0	3.4137843125917913	4.152288388807123E-4
66-67	5.170774220023965E-4	9.24471754489133E-4	0.0	3.4202712838860028	4.2306334527468796E-4
68-69	5.170774220023965E-4	9.79313299246963E-4	0.0	3.42641353689888	4.3089785166866367E-4
70-71	5.327464347903478E-4	0.00100281681842889	0.0	3.4315138005613584	4.700703836385422E-4
72-73	5.79753473154202E-4	0.00100281681842889	0.0	3.435368377707194	5.170774220023965E-4
74-75	5.79753473154202E-4	0.0010106513248228657	0.0	3.438306317604935	5.170774220023965E-4
76-77	5.79753473154202E-4	0.0010419893503987685	0.0	3.4413696096049797	5.170774220023965E-4
78-79	5.79753473154202E-4	0.0010654928695806956	0.0	3.4434535883057773	5.170774220023965E-4
80-81	5.79753473154202E-4	0.0010654928695806956	0.0	3.4441821974004165	5.249119283963721E-4
82-83	5.79753473154202E-4	0.0010654928695806956	0.0	3.444699274822419	5.484154475782992E-4
84-85	5.79753473154202E-4	0.0010654928695806956	0.0	3.444902971988663	5.484154475782992E-4
86-87	5.79753473154202E-4	0.001081161882368647	0.0	3.4450204895845724	5.484154475782992E-4
88	5.79753473154202E-4	0.001159506946308404	0.0	3.44503615859736	5.640844603662506E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7385	0.0	50.875614	1
GTATCAA	14390	0.0	38.80419	1
TCAACGC	17865	0.0	31.97691	4
ATCAACG	18045	0.0	31.586151	3
CAACGCA	18425	0.0	31.00502	5
TATCAAC	18925	0.0	30.509594	2
AACGCAG	18965	0.0	30.122198	6
ACGCAGA	21400	0.0	26.674564	7
CGCAGAG	22015	0.0	25.870558	8
GCAGAGT	25645	0.0	22.107603	9
GTGGTAT	3640	0.0	21.71154	1
ATCTCGT	12605	0.0	21.51586	94
TGGTATC	3695	0.0	20.803982	2
GAGTACT	15065	0.0	20.679825	12-13
AGAGTAC	23075	0.0	19.929094	10-11
TACATGG	17965	0.0	19.25512	2
CAGAGTA	25010	0.0	19.19002	10-11
GTACATG	18125	0.0	19.085142	1
ACATGGG	17915	0.0	18.9446	3
GTACTTT	16795	0.0	18.549807	14-15
>>END_MODULE